Diplomová práce se zabývá hybridním vyšetřením fMRI-EEG. V EEG signálu jsou přítomny dva základní artefakty, gradientní a balistokardiografický. Po odstranění gradientního artefaktu a detekce R vln v kanálu EKG byl navržen postup pro odstranění balistokardiografického artefaktu, pomocí metody odečtu artefaktového vzoru, pomocí metody ICA (metody nezávislých proměnných) a metody spojující obě uvedené. Byla vyhodnocena úspěšnost všech metod a jejich vliv na evokované potenciály v signálu EEG.

The new approach for motion artifact suppression in optical action potential records is presented in this thesis. Presented approach is based on independent component analysis utilization. Efficiency of proposed approach is evaluated here as well as its comparison with state of the art motion artifact suppression approaches.

In the semiconductor industry the reliability of devices is of paramount importance. Therefore, after removing the defective ones, one wants to detect irregularities in measurement data because corresponding devices have a higher risk of failure early in the product lifetime. The paper presents a method to improve the detection of such suspicious devices where the screening is made on transformed measurement data. Thereby, e.g., dependencies between tests can be taken into account. Additionally, a new dimensionality reduction is performed within the transformation, so that the reduced and transformed data comprises only the informative content from the raw data. This simplifies the complexity of the subsequent screening steps. The new approach will be applied to semiconductor measurement data and it will be shown, by means of examples, how the screening can be improved.

Le rétinoblastome est un cancer pédiatrique rare de la rétine en cours de développement. Si dans les pays développés, le taux de survie avoisine 100%, une énucléation de l’oeil atteint est cependant nécessaire dans plus de 70% des cas.En 1971, Knudson émit l’hypothèse des deux “hits”, qui permit de comprendre que le rétinoblastome s’initie généralement après une perte bi-allélique du gène RB1. Cependant, les autres mécanismes moléculaires qui régissent ce cancer restent depuis peu connus. Par exemple, peu d’études génomiques ont été conduites. Ainsi, la nature de la cellule d’origine, ainsi que la présence ou non d’une hétérogénéité intertumorale, font encore débat. Dans cette étude, nous avons dressé un portrait génomique et clinique complet du rétinoblastome; plusieurs observations ont montré qu’il s’agit bien d’une maladie hétérogène, avec deux sous-types distincts. Nous avons d’abord identifié les deux sous-types avec à une approche couplant une analyse en composantes indépendantes (ACI) de transcriptomes tumoraux avec des marquages immunohistochimiques. Les rétinoblastomes du premier sous-type, dits “cone-like” expriment uniformément des marqueurs de cônes, tandis que ceux du second sous-type, dits “bivalent-type”, ont une forte hétérogénéité intratumorale, avec un enchevêtrement de zones de différenciation ganglionnaire ou cône. Grâce à une étude plus approfondie des transcriptomes et de données d’altérations génomiques, nous avons ensuite montré que les sous-types dépendent de voies de signalisation et d’oncogènes différents. Les bivalent-type ont notamment une présence quasi-systématique de gains de MDM4 ou d’amplifications de MYCN. Nous nous sommes ensuite tournés vers les méthylomes des rétinoblastomes, et constaté une forte hétérogénéité entre les sous-types. Nous avons décomposé cette hétérogénéité grâce à une ACI, et constaté qu’elle n’était pas liée uniquement à la différenciation cône ou ganglion. Nous avons ensuite étudié les données cliniques de la cohorte, et constaté que les sous-types avaient des âges au diagnostic et des formes de croissance différents, les tumeurs cone-like se developpant généralement chez des patients jeunes avec des tumeurs exophytiques, et les bivalent-type chez des patients plus âgés avec des tumeurs endophytiques. De plus, les patients avec des inactivations constitutionnelles du gène RB1 développent majoritairement des tumeurs cone-like; les cone-like s’initieraient donc plus tôt durant le développement de la rétine. Nous avons finalement séquencé les exomes de 74 paires tumeur-normal. Les rétinoblastomes avaient un taux de mutations extrêmement faible (0.1 mutations par mégabase), comme beaucoup de cancers pédiatriques. Nous avons identifié des mutations somatiques récurrentes dans RB1, BCOR et ARID1A. Ces gènes se trouvaient de plus dans des régions minimales de pertes chromosomiques. Surtout, les inactivations des deux gènes avaient souvent de fortes fréquences alléliques. Ceci indique que ces inactivations ont lieu précocément dans la tumorigénèse. En conclusion, notre étude a permis de dresser un premier portrait génomique complet du rétinoblastome, a révélé l’existence de deux sous-types distincts, ainsi que fourni des indices quant à la cellule d’origine de chaque sous-type, et les mécanismes moléculaires les régissant. / Retinoblastoma is a rare pediatric cancer of the developing retina. In high-income countries, survival rates near 100%; however, enucleation of the affected eye has to be performed in over 70% of patients. Knudson’s 1971 two-hit hypothesis led to the discovery that this cancer usually initiates after a bi-allelic loss of the RB1 gene. Despite this early finding, little is known about the other molecular underpinnings of retinoblastoma. For instance, few genome-wide studies have described the genetic and epigenetic characteristics of these tumors. Furthermore, there is still no clear consensus regarding this cancer’s cell of origin, or whether or not it is homogenous disease. In this study, we built a comprehensive molecular and clinical portrait of retinoblastoma. Several lines of evidence led us to conclude that retinoblastoma is in fact a heterogeneous disease, with two distinct subtypes. We first uncovered the subtypes through a strategy that coupled an independent component analysis (ICA) of tumor transcriptomes to tumor immunohistochemical stainings. Retinoblastomas of the first subtype, called “cone-like”, homogeneously display cone-like differentiation, while those of the second subtype, called “bivalent-type”, exhibit strong intratumoral heterogeneity, with areas of cone-like differentiation intertwined with areas of ganglion-like differentiation. Further analysis of the transcriptomic data, as well as of copy number alteration data revealed that both subtypes may rely on different pathways and oncogenes. We notably observed a quasi-systematic presence of MDM4 gains or MYCN amplifications in bivalent-type tumors. We next turned to retinoblastomas’ methylomes; these considerably varied between the subtypes. ICA allowed us to decompose this inter-subtype methylomic heterogeneity, which was found to go beyond methylation due to cone-like or ganglion-like differentiation. We next studied the tumors’ clinical data, and found that cone-like tumors are most often diagnosed in very young patients with exophytic tumor growth, while bivalent-type tumors are found in older patients with endophytic tumor growth. Furthermore, patients with germline inactivations of RB1 mostly developed cone-like retinoblastomas, indicating that these tumors may initiate earlier during retinal development. In the final part of our study, we performed whole exome sequencing of 74 tumor-normal pairs. Like many pediatric cancers, the tumors had very low background mutation rates (0.1 mutations per megabase). Recurrent somatic mutations were found in RB1, BCOR and ARID1A, and these genes were also found to be in minimal regions of chromosomal losses. Importantly, both inactivations often had very high allelic frequencies, indicating that these events occur very early on in retinoblastoma tumorigenesis.Taken together, our study outlines a first comprehensive genomic portrait of retinoblastomas, points to the existence of two distinct subtypes, and provides insights into the cells-or-origin and the molecular mechanisms underlying these subtypes.

No description available.

In wireless communications, frequency-selective fading is a major source of impairment for wireless communications. In this research, a novel Frequency-Domain Independent Component Analysis (ICA-F) approach is proposed to blindly separate and deconvolve signals traveling through frequency-selective, slow fading channels. Compared with existing time-domain approaches, the ICA-F is computationally efficient and possesses fast convergence properties. Simulation results confirm the effectiveness of the proposed ICA-F. Orthogonal Frequency Division Multiplexing (OFDM) systems are widely used in wireless communications nowadays. However, OFDM systems are very sensitive to Carrier Frequency Offset (CFO). Thus, an accurate CFO compensation technique is required in order to achieve acceptable performance. In this dissertation, two novel blind approaches are proposed to estimate and compensate for CFO within the range of half subcarrier spacing: a Maximum Likelihood CFO Correction approach (ML-CFOC), and a high-performance, low-computation Blind CFO Estimator (BCFOE). The Bit Error Rate (BER) improvement of the ML-CFOC is achieved at the expense of a modest increase in the computational requirements without sacrificing the system bandwidth or increasing the hardware complexity. The BCFOE outperforms the existing blind CFO estimator [25, 128], referred to as the YG-CFO estimator, in terms of BER and Mean Square Error (MSE), without increasing the computational complexity, sacrificing the system bandwidth, or increasing the hardware complexity. While both proposed techniques outperform the YG-CFO estimator, the BCFOE is better than the ML-CFOC technique. Extensive simulation results illustrate the performance of the ML-CFOC and BCFOE approaches.

No description available.

No description available.

In recent years, researchers have increasingly used magnetic resonance imaging (MRI) to study temporal skeletal muscle changes using gradient echo (GRE) echo planar imaging (EPI). These studies, typically involving exercise or ischemic challenges, have differentiated healthy subjects from athletic or unhealthy populations, such as those with peripheral vascular disease. However, the analysis methodologies have been lacking. In this thesis, two sessions of post-exercise GRE EPI data were collected from six subjects' lower legs using a 3 Tesla MRI scanner and a custom built ergometer. Past studies used common medical imaging software for motion correction. This work shows that such tools degrade leg image data by introducing motion, increasing root mean squared error in rest data by 22%. A new approach decreased it by 12%. EPI distortion correction in muscle images was also achieved, with the correlation ratio of functional and structural images increasing by up to 8%. In addition, a brief but intense artifact in GRE EPI muscle images results from muscle tissue moving in and out of the imaged volume. This through-plane artifact was successfully modelled as a mono-exponential decay for regression analysis, increasing the utility of the residual signal. The regression parameters were also leveraged to produce muscle displacement maps, identifying 44% of voxels as displaced. The maps were validated in a motion phantom and in-vivo using ultrasound. Finally, independent component analysis (ICA) was applied to post-exercise GRE EPI images to detect features in a data-driven, multivariate way and improve on conventional ROI selection methods. ICA produced parametric maps that were spatially correlated to working muscles from every trial (most with |R| > 0.4). The components were also separated from the susceptibility, motion, and blood vessel signals, and temporally reliable within individuals. These methodological advances represent increased rigour in the analysis of muscle GRE EPI images. / Thesis / Doctor of Philosophy (PhD) / Adequate blood circulation to muscles is important for good health. Researchers have used magnetic resonance imaging (MRI) techniques to assess blood and oxygen supply to muscles. The work in this thesis improves upon the analysis methods in prior work, especially in the areas of motion correction of the images and selection of individual muscle regions for analysis. Previous techniques could sometimes make motion in muscle images worse. This work provides valuable motion and distortion correction for muscle imaging, ensuring that measurements truly reflect muscle physiology. It also describes a method to remove an unwanted signal from post-exercise muscle data, and create a map of the internal muscle motion that occurred. Finally, an advanced mathematical technique was used to extract signals of interest and important spatial features from muscle image data automatically. The technique produced reliable results within and among subjects.

Στο πλαίσιο της εργασίας αυτής πραγματοποιείται ανάλυση και εφαρμογή του διαχωρισμού ακουστικών σημάτων, τα οποία έχουν ληφθεί από το ανθρώπινο σώμα, όταν αυτό βρίσκεται σε κατάσταση ύπνου. Τα σήματα αυτά έχουν ληφθεί με τη βοήθεια μιας συσκευής πιεζοκρυστάλλων και ο διαχωρισμός τους επιτυγχάνεται με τη μέθοδο Ανάλυσης Ανεξάρτητων Συνιστωσών (ICA). Κύριος σκοπός όλων των παραπάνω είναι να χρησιμοποιηθεί η εν λόγω μεθοδολογία στη διάγνωση της αποφρακτικής άπνοιας (OSA). Στο πρώτο κεφάλαιο, παρουσιάζεται αναλυτικά η μέθοδος ICA και το μαθηματικό μοντέλο που την περιγράφει, όπως επίσης και όλα τα βήματα προεπεξεργασίας της. Στη συνέχεια αναλύεται διεξοδικά η λειτουργία του αλγορίθμου FastICA και οι ιδιότητες του, με τον οποίο υλοποιείται το πειραματικό μέρος της εργασίας αυτής. Στο δεύτερο κεφάλαιο, μελετάται η ασθένεια της αποφρακτικής άπνοιας (OSA), οι παράγοντες και η παθολογία της καθώς και το κύριο διαγνωστικό σύμπτωμα της: το ροχαλητό. Ύστερα, πραγματεύεται την διάγνωση και τους γνωστότερους τρόπους θεραπείας αυτής της νόσου και τελικά τη μέθοδο του Snoring Detection. Στο τρίτο κεφάλαιο γίνεται μια εισαγωγή στον πιεζοηλεκτρισμό, και μία μελέτη του πιεζοηλεκτρικού φαινομένου και του μαθηματικού του μοντέλου. Ακολουθεί αναφορά των ειδών πιεζοηλεκτρικών αισθητήρων με τους οποίους λαμβάνονται τα σήματα που εξετάζονται σε αυτή την εργασία. Στο επόμενο κεφάλαιο γίνεται μία σύνδεση των δεδομένων θεωρίας που αναφέρονται στα προηγούμενα κεφάλαια και μία εισαγωγή στην πειραματική μέθοδο. Στο κεφάλαιο πέντε παρατίθενται κάποια παραδείγματα εφαρμογής του αλγορίθμου FastICA με τυχαία σήματα, τα οποία έχουν σκοπό να δοκιμάσουν την απόδοση του. Στο κεφάλαιο έξι, 5 γίνεται η πειραματική διαδικασία όπου τώρα τα σήματα που διαχωρίζονται με τον αλγόριθμο FastICA προέρχονται από το ανθρώπινο σώμα. Η υλοποίηση της γίνεται σε Matlab. Έτσι, γίνεται εξαγωγή του ζητούμενου σήματος ροχαλητού και αναγράφονται κάποια συμπεράσματα για την απόδοση του αλγορίθμου. Στο τέλος της εργασίας παρατίθενται σε ένα παράρτημα όλοι οι κώδικες της MATLAB που χρησιμοποιήθηκαν για την ολοκλήρωση του πειραματικού της μέρους στα κεφάλαια πέντε και έξι. / In this particular thesis, analysis and application of separation of acoustic signals is carried out. These signals have been taken from the human body in a sleeping state. They are obtained by means of a piezocrystallic device and their separation is achieved by the method of Independent Component Analysis (ICA). The main purpose of all this is to use this methodology in order to diagnose the Obstructive Sleep Apnea (OSA). The first chapter presents the method of ICA and the mathematical model that describes it as well as all the pre-processing steps. Then it analyses, in detail, the algorithm FastICA, which is used in the experimental part of this thesis and its properties. The second chapter studies the disease of obstructive sleep apnea (OSA), its factors and its pathology and the major diagnostic symptom: snoring. Then, it discusses the diagnosis and the best known ways of treating this disease and eventually the method of Snoring Detection. The third chapter is an introduction to piezoelectricity and a study of the piezoelectric effect and its mathematical description. This is followed by a reference to the types of piezoelectric sensors which are used to obtain the signals used in this paper. In chapter five we have listed some examplesapplications of the FastICA algorithm with random signals, which are designed to test the performance. Section six is where the experimental procedure takes place. The signals derived from the human body are separated by the algorithm FastICA and the implementation is done in Matlab. In addition, some conclusions regarding the performance of the algorithm. At the end of this paper, all the MATLAB codes used for the completion of the experimental part of the chapters five and six are listed in an Annex.