Thesis (M. Med. Sc.)--University of Hong Kong, 2005.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xvi, 145 p.; also includes graphics (some col.). Includes bibliographical references (p. 121-145). Available online via OhioLINK's ETD Center

Tierärztl. Hochsch., Diss., 2004--Hannover.

Tierärztl. Hochsch., Diss., 2004--Hannover.

No description available.

Thesis (M.A.)--Boston University / We have tried to determine insulin in urine and plasma by means of aminoguanidine sulfate solution. The urine and plasma are cleared with Somogyi reagent. The filtrate is then diluted with water to the range of the aminoguanidine sulfate reagent. Our only difficulty lies in the compliance of the curves to Beer's law. The curves tend to give less color in the higher concentrations and more color in the lower concentrations thus the curves do not pass through or near the origin. It is our fond hope that if the Beer's law plots were straightened out the determination would be successful. By running determinations simultaneously at two different temperatures, it would then be possible to determine insulin and only insulin.

Thesis (Ph.D.)--Boston University / It is now generally recognized that insulin facilitates the transfer of certain sugars through cytostructural barriers. If the transfer of a utilizable sugar into the cell is affected by a rate-limiting reaction, then all subsequent enzymatic reactions of this sugar in the cell would be dependent upon this reaction. This study has involved an investigation of factors which regulate the transfer of sugar into muscle cells. [TRUNCATED]

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The rate of glucose stimulated in vitro (pro)insulin synthesis in islets of Langerhans isolated from fed male rats was determined. Increases in the glucose concentration of the incubation medium, over the physiological range (2 to 20 mM), stimulated the rates of both (pro)insulin and total protein synthesis during a two hour incubation. This stimulation was preferential for (pro)insulin synthesis and had a sigmoidal dose response curve with a concentration threshold of between 2.5 mM and 5.0 mM and a maximal rate at 20 mM glucose. Inhibition of islet RNA synthesis by.;ctinomycin D depressedthe rate of total protein synthesis within 30 minutes of the application of a 20 mM glucose stimulus. A specific inhibition of (pro)insulin synthesis by actinomycin D, that occured 60 minutes after the application of a 20 mM glucose stimulus, was thought to reflect the inhibition of glucose stimulated preproinsulin mRNA synthesis. The effect of glucose on islet preproinsulin mRNA content during in vitro incubations was also determined. To achieve this a dot blot hybridization assay for preproinsulin mRNA was 3 2 developed which used a P-labeled cloned human insulin gene as the hybridization probe. The assay proved to be sensitive enough to detect preproinsulin mRNA in as few as fifty islets. Incubation of islets, for 2 hours, at 2.5 mM glucose had no effect on islet preproinsulin mRNA content but incubation at 20 mM glucose increased islet preproinsulin mRNA content. Actinomycin D had no effect on this pattern of glucose stimulation and it is suggested that the degradation of preproinsulin mRNA is in some way dependent on RNA synthesis and that its inhibition by glucose plays an important role in the regulation of islet preproinsulin mRNA content.