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High-Glucose-Induced Regulation of Intracellular ANG II Synthesis and Nuclear Redistribution in Cardiac MyocytesSingh, Vivek P., Le, Bao, Bhat, Vadiraja B., Baker, Kenneth M., Kumar, Rajesh 01 August 2007 (has links)
The prevailing paradigm is that cardiac ANG II is synthesized in the extracellular space from components of the circulating and/or local renin-angiotensin system. The recent discovery of intracrine effects of ANG II led us to determine whether ANG II is synthesized intracellularly in neonatal rat ventricular myocytes (NRVM). NRVM, incubated in serum-free medium, were exposed to isoproterenol or high glucose in the absence or presence of candesartan, which was used to prevent angiotensin type 1 (AT1) receptor-mediated internalization of ANG II. ANG II was measured in cell lysates and the culture medium, which represented intra- and extracellularly synthesized ANG II, respectively. Isoproterenol increased ANG II concentration in cell lysates and medium of NRVM in the absence or presence of candesartan. High glucose markedly increased ANG II synthesis only in cell lysates in the absence and presence of candesartan. Western analysis showed increased intracellular levels of angiotensinogen, renin, and chymase in high-glucose-exposed cells. Confocal immunofluorocytometry confirmed the presence of ANG II in the cytoplasm and nucleus of high-glucose-exposed NRVM and along the actin filaments in isoproterenol-exposed cells. ANG II synthesis was dependent on renin and chymase in high-glucose-exposed cells and on renin and angiotensin-converting enzyme in isoproterenol-exposed cells. In summary, the site of ANG II synthesis, intracellular localization, and the synthetic pathway in NRVM are stimulus dependent. Significantly, NRVM synthesized and retained ANG II intracellularly, which redistributed to the nucleus under high-glucose conditions, suggesting a role for an intracrine mechanism in diabetic conditions.
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Activation of the Intracellular Renin-Angiotensin System in Cardiac Fibroblasts by High Glucose: Role in Extracellular Matrix ProductionSingh, Vivek, Baker, Kenneth M., Kumar, Rajesh 01 April 2008 (has links)
The occurrence of a functional intracellular renin-angiotensin system (RAS) has emerged as a new paradigm. Recently, we and others demonstrated intracellular synthesis of ANG II in cardiac myocytes and vascular smooth muscle cells that was dramatically stimulated in high glucose conditions. Cardiac fibroblasts significantly contribute to diabetes-induced diastolic dysfunction. The objective of the present study was to determine the existence of the intracellular RAS in cardiac fibroblasts and its role in extracellular matrix deposition. Neonatal rat ventricular fibroblasts were serum starved and exposed to isoproterenol or high glucose in the absence or presence of candesartan, which was used to prevent receptor-mediated uptake of ANG II. Under these conditions, an increase in ANG II levels in the cell lysate represented intracellular synthesis. Both isoproterenol and high glucose significantly increased intracellular ANG II levels. Confocal microscopy revealed perinuclear and nuclear distribution of intracellular ANG II. Consistent with intracellular synthesis, Western analysis showed increased intracellular levels of renin following stimulation with isoproterenol and high glucose. ANG II synthesis was catalyzed by renin and angiotensin-converting enzyme (ACE), but not chymase, as determined using specific inhibitors. High glucose resulted in increased transforming growth factor-β and collagen-1 synthesis by cardiac fibroblasts that was partially inhibited by candesartan but completely prevented by renin and ACE inhibitors. In conclusion, cardiac fibroblasts contain a functional intracellular RAS that participates in extracellular matrix formation in high glucose conditions, an observation that may be helpful in developing an appropriate therapeutic strategy in diabetic conditions.
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La protéine apparentée à l'hormone parathyroïdienne (PTHrP) dans la biologie de la cellule mésangiale : rôles dans l'inflammation, la croissance et la survie / The parathyroid hormone-related protein (PTHrP) in the biology of the mesangial cell : roles in inflammation, growth and survivalHochane, Mazène 28 September 2012 (has links)
La glomérulonéphrite mésangioproliférative (GNMP) se caractérise par une inflammation locale et la prolifération et l’apoptose des cellules mésangiales (CM). La protéine apparentée à l’hormone parathyroïdienne (PTHrP) a été impliquée dans ces processus dans divers types cellulaires. Nous avons analysé les effets de la PTHrP sur ces processus dans les CM. Nous montrons que la PTHrP majore la prolifération des CM par voie intracrine et diminue leur apoptose par voie paracrine. La PTHrP stimule les voies de l’AMPc/PKA et PI3-K/Akt conduisant à l’activation du NFkB et à la majoration de la cyclooxygénase-2 (Cox-2). La Cox-2 était responsable de la survie des CM par la PTHrP. Par ailleurs, l’IL-1beta et le TNF-alpha majorent l’expression de la PTHrP dans les CM, et la PTHrP elle-même induisait l’expression de cytokines et chimiokines. L’expression des cytokines (IL-17, IL-16), était brève (pic à 2h). L’expression des chimiokine (RANTES, MIP-2, TARC et I-TAC) était plus prolongée (4h). Dans un modèle murin de GNMP, la PTHrP était surexprimée à J1 dans les glomérules malades. Elle pourrait contribuer à l’inflammation locale, à la prolifération et à la survie des CM. / Mesangial proliferative glomerulonephritis (MPGN) is characterized by mesangial cells (MC) inflammation, proliferation and apoptosis. The parathyroid hormone-related protein (PTHrP) is known to influence these processes in many cell types. In this work we analyzed the effects of PTHrP on MC proliferation, apoptosis and inflammation. Our results show that PTHrP induced MC proliferation through the intracrine pathway while it promoted their survival through the paracrine one. PTHrP activating its receptor PTH1R, led to the activation of cAMP/PKA and PI3-K/Akt pathways, which induced NF-kappaB, and upregulated the cyclooxygenase-2 (Cox-2). We have shown that the Cox-2 was responsible of the anti-apoptotic effect of PTHrP on MC. Otherwise, IL-1beta and TNF-alpha importantly upregulated the PTHrP in MC and PTHrP itself led to an overexpression of many cytokines and chemokines. The overexpression of cytokines (IL-17 and IL-16) was brief (2h) while that of chemokines was extended (4h). In a mouse model of MPGN, PTHrP was upregulated in the injured glomeruli at day 1. PTHrP may then contribute to the inflammation, the proliferation and the survival of MC.
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Etude de la voie d’action intracrine et nucléaire du Fibroblast Growth Factor 1 dans des cellules de type neuronal / Characterization of Fibroblast Growth Factor 1 intracrine and nuclear pathway in neuronal cellsPirou, Caroline 25 November 2016 (has links)
Le FGF1 est un facteur de croissancenon sécrété, localisé dans le cytosol et le noyau.Il induit la prolifération, la différenciation et lasurvie cellulaires. Il est également impliqué dansla progression tumorale et peut conférer unechimiorésistance aux tumeurs qui lesurexpriment.Notre équipe s’intéresse depuis plusieursannées aux activités intracrines du FGF1 en tantque régulateur de la différenciation et de lasurvie cellulaires. L’équipe a ainsi montré que leFGF1 intracellulaire a une activité antiapoptotiquedans plusieurs modèles cellulaires :cellules REtsAF (fibroblastes de rat), PC12(phéochromocytome de rat), SH-SY5Y(neuroblastome humain) suite à l’induction del’apoptose dépendante de p53 via un stressgénotoxique. Dans les cellules PC12, un modèled’étude de la différenciation neuronale, le FGF1possède également une activité neurotrophique.À l’aide de différentes formes mutantes duFGF1, nous avons cherché à mieux caractériserles mécanismes régulant son activité antiapoptotiquedans les cellules PC12 et SH-SY5Y,ainsi que son activité neurotrophique dans lescellules PC12. Nous avons pu montrer que ledomaine C-terminal du FGF1 ainsi que sesmodifications post-traductionnelles sontimportants pour la régulation de ses activitésintracellulaires ; et que la phosphorylation duFGF1 inhibe son activité anti-apoptotique dansles cellules PC12 et SH-SY5Y mais ne régule passon activité de différenciation dans les cellulesPC12.Nos résultats montrent donc pour lapremière fois le rôle de la phosphorylation dansla régulation des activités du FGF1 et ce dansdeux modèles cellulaires de rat et humain. / .FGF1 is a non-secreted cytosolic andnuclear growth factor, inducing cell proliferation,differentiation and survival. FGF1 is also involvedin tumor progression and can inducechemoresistance in FGF1 overexpressing tumors.Our team has focused for several years onthe FGF1 intracrine neurotrophic and survivalactivities. The team has shown that intracellularFGF1 is an anti-apoptotic factor in several celllines : REtsAF (rat fibroblasts), PC12 (derivedfrom a rat pheochromocytoma) and SH-SY5Ycells (derived from a human neuroblastoma)following the induction of p53-dependentapoptosis via a genotoxic stress. In PC12 cells, amodel of neuronal differentiation, FGF1 is also aneurotrophic factor.We aimed to better characterize themechanisms regulating the anti-apoptoticactivity of FGF1 by expressing various mutantforms of FGF1 in PC12 and SH-SY5Y cell lines. Wehave shown that FGF1 C-terminal domain and itspost-translational modifications are importantfor the regulation of its intracellular activities ;and that FGF1 phosphorylation inhibits its antiapoptoticactivities in both PC12 and SH-SY5Ycell lines, but does not affect its differentiationactivity in PC12 cells.Thus, our results showed for the first time thecrucial role of FGF1 phosphorylation in theregulation of its intracrine anti-apoptotic activityin both rat and human cellular models.
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Studies on signals mediating or preventing the intracrine induction of chromatin compaction and cell death by high molecular weight fibroblast growth factor 2Ma, Xin 05 April 2011 (has links)
Fibroblast growth factor 2 (FGF2) is a multifunctional protein translated as CUG-initiated, high molecular weight (hi FGF2) or AUG-initiated, low molecular weight (lo FGF2) isoforms with potentially distinct functions. Previous work showed that overexpression of hi- but not lo FGF2 elicited chromatin compaction resulting in cell death, by an intracrine route. A series of studies were undertaken aimed at extending our understanding of the intracrine action of Hi FGF2. Major findings are as follows:
a. Hi FGF2 overexpression induces apoptotic cell death, as indicated by increased TUNEL staining, and mitochondrial participation (cytochrome c release to cytosol, rescue of the hi FGF2 phenotype by the anti-apoptotic protein Bcl-2.
b. Increased expression of pro-survival signals/proteins that are known to upregulate Bcl-2, such as nuclear Akt; the PIM-1 kinase; and the heat shock protein hsp70, also rescued the hi FGF2-induced phenotype.
c. The hi-FGF2 effect was associated with sustained, intracrine, activation of ERK, and was blocked by ERK inhibitors.
d. FGF2 isoform specific affinity chromatography followed by mass spectroscopy identified several proteins as potentially interacting with hi FGF2; of these, the p68 RNA helicase and the hsp70 were further confirmed as interacting partners, by co-immunoprecipitation.
e. Increased nuclear co-localization, and possibly interaction, between hi FGF2 and overexpressed hsp70 correlated with rescue from hi FGF2 induced cell death.
f. Factors associated with cardiac pathology (isoproterenol, angiotensin II, endothelin I) also upregulated endogenous hi FGF2 in cardiac cells in culture. Adriamycin-induced cardiotoxicity in the rat, known to be linked to increased incidence of apoptosis, was also associated with increased endogenous hi FGF2.
g. Hi FGF2 is expressed in the human heart (atria) and localizes in both cytosol and nuclei, suggesting a participation in human heart physiology and pathophysiology.
Work presented here is consistent with the notion that endogenous hi FGF2 up-regulation may play a role in promoting cell death during prolonged tissue stress and dysfunction. It follows that processes related to hi FGF2 upregulation, hi FGF2-nuclear protein interactions and mechanisms of hi FGF2 induced cell death, represent potential therapeutic targets for modulating cell death.
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Studies on signals mediating or preventing the intracrine induction of chromatin compaction and cell death by high molecular weight fibroblast growth factor 2Ma, Xin 05 April 2011 (has links)
Fibroblast growth factor 2 (FGF2) is a multifunctional protein translated as CUG-initiated, high molecular weight (hi FGF2) or AUG-initiated, low molecular weight (lo FGF2) isoforms with potentially distinct functions. Previous work showed that overexpression of hi- but not lo FGF2 elicited chromatin compaction resulting in cell death, by an intracrine route. A series of studies were undertaken aimed at extending our understanding of the intracrine action of Hi FGF2. Major findings are as follows:
a. Hi FGF2 overexpression induces apoptotic cell death, as indicated by increased TUNEL staining, and mitochondrial participation (cytochrome c release to cytosol, rescue of the hi FGF2 phenotype by the anti-apoptotic protein Bcl-2.
b. Increased expression of pro-survival signals/proteins that are known to upregulate Bcl-2, such as nuclear Akt; the PIM-1 kinase; and the heat shock protein hsp70, also rescued the hi FGF2-induced phenotype.
c. The hi-FGF2 effect was associated with sustained, intracrine, activation of ERK, and was blocked by ERK inhibitors.
d. FGF2 isoform specific affinity chromatography followed by mass spectroscopy identified several proteins as potentially interacting with hi FGF2; of these, the p68 RNA helicase and the hsp70 were further confirmed as interacting partners, by co-immunoprecipitation.
e. Increased nuclear co-localization, and possibly interaction, between hi FGF2 and overexpressed hsp70 correlated with rescue from hi FGF2 induced cell death.
f. Factors associated with cardiac pathology (isoproterenol, angiotensin II, endothelin I) also upregulated endogenous hi FGF2 in cardiac cells in culture. Adriamycin-induced cardiotoxicity in the rat, known to be linked to increased incidence of apoptosis, was also associated with increased endogenous hi FGF2.
g. Hi FGF2 is expressed in the human heart (atria) and localizes in both cytosol and nuclei, suggesting a participation in human heart physiology and pathophysiology.
Work presented here is consistent with the notion that endogenous hi FGF2 up-regulation may play a role in promoting cell death during prolonged tissue stress and dysfunction. It follows that processes related to hi FGF2 upregulation, hi FGF2-nuclear protein interactions and mechanisms of hi FGF2 induced cell death, represent potential therapeutic targets for modulating cell death.
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La protéine apparentée à l'hormone parathyroïdienne (PTHrP) dans la biologie de la cellule mésangiale : rôles dans l'inflammation, la croissance et la survieHochane, Mazène 28 September 2012 (has links) (PDF)
La glomérulonéphrite mésangioproliférative (GNMP) se caractérise par une inflammation locale et la prolifération et l'apoptose des cellules mésangiales (CM). La protéine apparentée à l'hormone parathyroïdienne (PTHrP) a été impliquée dans ces processus dans divers types cellulaires. Nous avons analysé les effets de la PTHrP sur ces processus dans les CM. Nous montrons que la PTHrP majore la prolifération des CM par voie intracrine et diminue leur apoptose par voie paracrine. La PTHrP stimule les voies de l'AMPc/PKA et PI3-K/Akt conduisant à l'activation du NFkB et à la majoration de la cyclooxygénase-2 (Cox-2). La Cox-2 était responsable de la survie des CM par la PTHrP. Par ailleurs, l'IL-1beta et le TNF-alpha majorent l'expression de la PTHrP dans les CM, et la PTHrP elle-même induisait l'expression de cytokines et chimiokines. L'expression des cytokines (IL-17, IL-16), était brève (pic à 2h). L'expression des chimiokine (RANTES, MIP-2, TARC et I-TAC) était plus prolongée (4h). Dans un modèle murin de GNMP, la PTHrP était surexprimée à J1 dans les glomérules malades. Elle pourrait contribuer à l'inflammation locale, à la prolifération et à la survie des CM.
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Interaktom N-terminální domény IL-1α / Interactome of IL-1α N-terminal domainDolečková, Denisa January 2011 (has links)
Interactome of IL-1α N-terminal domain Cytokines are highly effective mediators produced by various cell types within and outside of the immune system with the aim to influence the orientation, intensity, and duration of the immune response and inflammatory process. Their biological effects mediated through binding the high-affinity membrane receptors and triggering the signal transduction pathway are usually well defined. However, as it is more and more frequently observed, in addition to the exocrine function, some cytokines may show intracrine effects. For this type of cytokines, the term "dual function cytokines" has been adopted. One of these cytokines is Interleukin-1α, in which the recent research has concentrated on determining its intracellular functions. The intracellular function of interleukin-1α has not been clearly defined so far. However, apart from the absence of the conventional hydrophobic sequence, its existence is supported by the fact that the N-terminal peptide included in its precursor is highly conserved and contains nuclear localization signal. The aim of this work is to define the conditions of localization of the interleukin-1α N- terminal domain in different cellular compartments and to study proteins potentially interacting with it using fluorescent microscopy. Key words:...
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