This master thesis offers two cost-saving measures to reduce the energy consumption of a residential building. These measure include insulation of the building envelope, domestic hot water by solar colectors and heat recovery.

This thesis deals with the quality of the external and internal environment and the possibility of interaction. Resolves components, pollutants and sources of pollutants that are contained in the external and internal air and thus influence around us all. The theoretical part describes the resources, limit concentration or additional filtering of the individual substances. Are summarized in detail the possibility of air exchange and ventilation in buildings intended for public gatherings. Another part of the treatment methods solve indoor environmental quality (grade of primary school) according to the changes of air flow, ventilation options and the impact of external environment on indoor air quality. It also includes a proposal for forced ventilation of the building of the school. In the experimental part, I focused on the evaluation of the concentration of carbon dioxide in the external and internal environment and the possibilities of noise pollution. In conclusion, there are variants commented and evaluated.

This disertation thesis is focused on the study of physico-chemical interactions of hyaluronan (with molecular weights from 10 to 1750 kDa) with cationic surfactants measured using uncommon technique named high resolution ultrasonic spectroscopy. Densitometer was also used for the study of these interactions, in measuring of density and ultrasonic velocity of hyaluronan with different molecular weight in dependence on elevated temperature (25 50 °C). The aim is the determination of critical micelle concentration (CMC) and critical aggregation concentration (CAC) of the suractants in the absence and in the presence of hyaluronan with various molecular weights. Interactions in this system are important for the design of the systems for the targeted delivery, especially for the drugs. The experiments were made in water and sodium chloride solution. The significant breakpoint in the ultrasonic velocity showed changes in the system hyaluronan-surfactant.

The first part of the final thesis is focused on the use of thermovision for diagnosing defects and failures in building industry. Documented history, physical principles and conditions for the measuring of thermographic camera are described in this part. Thermal images of the object described below were used for demonstrating the defects. The second part is dedicated to the elaborated energy assessment for a nine-storey slab block building. In total, there are five energy economic measures developed for the building. Three of those are of structural character and two of those are for building equipment. There are two variants designed based on the measures, one of which is recommended and evalu-ated. The final section is focused on the measurement of slab block building indoor environment (CO2, air temperature, relative humidity, dew point) and its assessment in concern with the current legislation.

The effect of elevated CO2 concentration (EC CO2) on photosynthesis has been observed on many hierarchical levels. There was a significant increase in the rate of photosynthesis of examined trees observed in previous studies thus I hypothesised these changes are accompanied by changes of chloroplast ultrastructure and photosystem content and function and the main aim of this study is to evaluate these adjustments. In this study 13 - 14 years old seedlings of Norway spruce (Picea abies L. Karst.) grown in glass domes with adjustable windows - one with ambient CO2 concentration as a control, the other one with simulated EC CO2 (700 ppm) - during the vegetative season were examined. Pigment content, fluorescence and reflectance indexes, activity of photosystem 1 (PS1) and 2 (PS 2) of isolated chloroplasts, size of cross-sectional area of chloroplast and proportion of stromal to granal thylakoids under EC CO2 treatment were investigated. Although there was a significant increase in the maximum rate of photosynthetic assimilation of trees from EC CO2 (observed by other researchers of our team), decreased chlorophyll and carotenoid content as well as the activity of both photosystems were observed, which is usually atributed to photosynthetic acclimation. As the rate of decrease of photosystem 1 and photosystem 2...

(EN) The formation of brain oedema, which accompanies ischemic or traumatic brain injuries, originates from a disruption of ionic/neurotransmitter homeostasis that leads to extracellular K+ elevation and neurotransmitter accumulation in the extracellular space. An increased uptake of these osmotically active substances, predominantly provided by astrocytes, is accompanied by intracellular water accumulation via aquaporin-4 (AQP4). Since it has been shown that the removal of perivascular AQP4 via the deletion of α- syntrophin, which is the protein responsible for anchoring AQP4 on the astrocytic membrane (Neely et al. 2001), delays oedema formation and K+ clearance (Amiry-Moghaddam et al. 2003), we aimed to elucidate how the alpha-syntrophin deletion affects astrocyte volume changes in the cortex during pathological states, such as hypoosmotic stress or oxygen- glucose deprivation (OGD), using three-dimensional (3D) confocal morphometry in situ. In order to visualize individual astrocytes that lack alpha-syntrophin, double transgenic mice (GFAP/EGFP/α-Syn-/- ) were generated by crossbreeding GFAP-EGFP mice with α- syntrophin knockout mice. 3D-confocal morphometry revealed that alpha-syntrophin deletion did not alter astrocyte swelling during hypoosmotic stress or their recovery in isotonic solution;...

This dissertation deals with the Official Development Assistance. The purpose of this dissertation was, first of all, to find out whether the target aid is focused on the poorest countries of the world. The theoretical part concentrates primarily on the institutional history of the Official Development Assistance and also on the development of mainstream theories which influenced it to the highest degree. In the analytical part the dissertation draws particularly from the database of the Development Assistance Committee under the Organisation for Economic Co-operation and Development. From the global point of view, the dissertation is focused especially on the quantitative analysis of the changes in the allocation of the Official Development Assistance in the period from 1989 to 2008. From the geographical point of view, the spatio-temporal change in territorial priorities of important bilateral and multilateral donors and also the dependence of their expenditure on the socio-economic maturity of aid beneficiaries was analysed. The ability to clearly define the territorial priorities and on their basis to effectively target the development assistance is in national context analysed on the example of the Czech Republic. From the local point of view, the dissertation gives a brief description of the...

Long-term evolution of nature-society interactions can be studied by using data of land use change. In the thesis is used a detailed statistical database of Czechia (developed at Charles University in Prague, Faculty of Science). The database allows monitoring changes of eight categories of land use in the period 1845-2000. Attention is devoted to the growing differentiation of land use at different scale levels, the thesis is concentrated on functional specialization of czech regions. Deepening division of labour between regions is reflected by increasing territorial concentration of land use. This process is monitored by using three methods. Ternary plots present an overview of the differentiation trends of land use. Spatial autocorrelation methods present the general development and specific areas of functional specialization in Czechia. A review by territorial heterogeneity rate provides a detailed look at the change of territorial concentration of land use. The thesis operates with three territorial classifications (hydrological, geomorphological and administrative) at two regional levels and the level of Czechia. Results are interpreted using the driving forces (such as technological development, economics, historical events, society). The final part of the thesis compares the development of...

A mathematical model of two-phase systems, such as clay suspensions, consists of a set of partial differential equations which reflect both the general laws of mechanics and the relations connecting the involved characteristics of the particular system under consideration. The latter equations are known as constitutive relations. The aim of this study was to find the constitutive relations for kaolin suspensions that are necessary when solving forward problems of fine sludge thickening processes. The task was to design and carry out experimental research of the given suspension and to find a convenient method of utilizing the results for the sake of getting the sought relationships. It follows from the applied mathematical theory of two-phase systems that the sought relationships are hydraulic conductivity of the suspension as a function of the solid-phase concentration and the dependence of the solid-phase concentration on the solid-phase stress. The first part of this study describes the experimental research. Since both the characteristics are difficult to measure, it was necessary to analyze the suspension's characteristics and their measurability. Subsequently, the process of the suspension preparation and the method of laboratory measurements were determined. The following sections present...

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