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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Zdeňka Čermáková Supervisor: Prof. RNDr. Jiří Lamka, CSc. Title of diploma thesis: The progression and results of the model parasite culturing liver fluke (Fasciola hepatica) in sheep (Ovis aries) Fasciolosis is a parasitic disease caused by liver fluke (Fasciola hepatica). This parasitosis is a significant disease in livestock, wild animals and in some parts of the world in humans. It causes a decrease in livestock performance and fertility, weight loss and high economic losses in livestock. The aim of this work was to describe in detail the course of artificial infection of sheep infected with F. hepatica metacercaria. Specifically, changes in blood count, egg excretion, and response of the immune system of infected individuals were monitored. The observed values were compared with the studies already performed. The demonstration of fasciolosis in artificially infected sheep was performed using the coprological sedimentation method, the differential determination of leukocytes from the blood layers, the determination of hematocrit and the immunological method ELISA (enzyme-linked immunosorbent assays). Three lambs of Texel breeds infected with 200 metacercaria were used for the...

The aim of this study was to isolate and extensively test promising candidate diatom species for biodiesel cultivation. Diatom strains were isolated from natural habitats in the state of Ohio (USA). These strains were tested to find optimal growing conditions and media (concentration of Nitrogen, Phosphorus, Silica, source of Nitrogen, temperature etc.).

Final thesis was worked out on theme Study of light sources for in vitro cultivation of the rootstock Gisela 5. Experiment was conducted alternately on Mendeleum Institute of Genetics and Plant breeding, Faculty of Horticulture in Lednice and on Fosfa a.s. company in Břeclav. The aim of this thesis was to expand knowledge about light sources and their influence on in vitro plant cultivation. Based on this information and knowledge, an experiment was then made to investigate the impact of three different LED light sources on in vitro cultivation of the rootstock Gisela 5 in the phase of multiplication and rooting.

Práce se zabývá virálním marketingem jako jednou ze složek komunikačního mixu na internetu. Představuje virální marketing i jako metodou kultivace návštěvnosti. V praktické části analyzuje některé uskutečněné virální kampaně. Shrnuje obecný postup při přípravě a realizaci virálních kampaní.

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