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The thesis was written at Mendel University in Brno, Faculty of Horticulture in Lednice at the Institute of viticulture and vinemaking. In this task, I dealt with various options of regulation the quantity grapes, suitable term for realization of regulation quantity grapevine and its effect for grapes quality. The task is separated into two parts. The literary part of the task deals with qualitative parameters of berry vine, agrotechnical interventions that affects the maturation and grapes quality, and possibilities of regulation the quantity grapes. The second part was experiment executed on the blue grape variety Lemberger. Regulation was done in five versions and two ways of quantity regulation. These regulated options were compared with the control option without regulation. The evaluation of the experimental results states, that the regulation of grapes in the variety Lemberger has a positive effect on the quality grapes. From the perspective of qualitative parameters appears to be the most suited option halving of grapes on the plant within 30 days after flowering. The correct timing of regulation and method of execution is very important. In conclusion, the regulation of quantity grapes should be an integral part of modern viticulture.

All over the world, the edible insects are considered a highly nutritious food with high protein and fat content. However, the nutritional value of insect is not constant. It can be affected by species, developmental stage, rearing technology or nutrition. Therefore, this thesis was aimed at obtaining the selected nutritional value of edible insects. Analyses were focused on the determination of the crude protein content, fat content, fatty acid profile and sterols in selected species of edible insects, which can be commonly reared in the Czech Republic. At the same time we also analysed samples of insects reared on the island of Sumatra to evaluate the influence of the climate on the nutritional value. The main aim of the thesis was the determination of optimal breeding conditions, developmental stages and feed rations for obtaining good production of insects with nutritional properties suitable for human nutrition. The analyses showed a high nutritional value of selected insect species, but also confirmed the significant differences in the content of individual nutrients between different species depending on the climate conditions and developmental stage. Comparing the fat and crude protein content in edible insects and other conventional sources of meat it has been found, that the examined insect is similar to beef concerning the fat and crude protein content. The results obtained are the basis for determining the appropriate rearing conditions and developmental stages for obtaining insect with the desired nutritional properties for human nutrition.

Thesis is focused on substance composition of wine, with the main focus on organic acid and possibilities of regulation, mainly with malic-lactic fermentation. In literary part is described substance composition of wine, with the main focus on organic acids, mainly tartaric acid, malic acid, lactic acid and citric acid. Further are described possibilities of acid regulation with increasing or decreasing of acids in wine. Deeply is describe decreasing of malic acid in red wines with help of apple-lactic fermentation. In experimental part are established testing variants of red wine variety Zweigeltrebe and Svätovavrinecké. By these varieties of wine were notice changes in quality parameters. Wines were observed during the malic-lactic fermentation and compared with wines without this fermentation. After this test was performed a sensory analysis. Result of analysis confirm, that malic-lactic fermentation has positive influence on the quality of wine. Wine is gently harmonious and fuller.

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The diploma thesis is dealing with searching in secondary structures of nucleic acids. The NA2Dsearch application was developed that allows to search databases of secondary structures for a structural motif. The application offers user-friendly graphical user interface where a query motif can be constructed in comfortable visual way using drag&drop, database and result structures can be browsed and visualized. The application contains original interactive nucleic acid structure visualization algorithm and novel search algorithm. The search algorithm can perform a motif search on structural data (unlike existing programs that can perform a motif search on sequence data). The motif search means that a query is created that describes the structure of motif of our interest with variabilities (e.g. the length of hairpin loop) that can be tolerated. Search results are ordered by the score computed according to the resemblance of hit and the query. The NA2Dsearch also supports searching in sequence data which are folded by external folding program. Searching capabilities are demonstrated on several search experiments involving HCV IRES and tRNA. Three searches for HCV IRES were performed: a search for HCV IRES structure in database of more than thousand of HCV genomic sequences, a folding analysis of HCV IRES...

Blood ethanol is a widely accepted marker for recent ethanol intake (within the last 4-6 h). However, the rapid elimination of ethanol from the body nearly always makes it impossible to assess ethanol ingestion beyond the most recent 6-8h. But serum fatty acid ethyl ester (FAEE) is detectable in the blood both when ethanol is presented and long after ethanol has been removed from the circulation. For this FAEE should be better marker for prior ethanol intake.

Creation of electronic materials for chemistry education Bc. Petra Váňová Faculty of Science, Charles University in Prague, 2014 The theme of my thesis is formation of educational instruments/tools for distance education and e-learning which at the same time can be inspirational for teachers of present learning. The theoretical section elaborates on the problems of distance education and e-learning. Furthermore, it describes Moodle, the system for teaching management. For the purpose of the applied section two teaching units were created during the class of Didactics of Organic Chemistry (MC280P06) at Department of Teaching and Didactics of Chemistry, Faculty of Science, Charles University in Prague. The two units focus on the problematic of carboxylic acids and their derivatives. Beside the information about these topics, the contents of the teaching units should provide the students with an insight into modern study tools which increase the effectiveness of distance education as well as present learning. The teaching units are supplemented by study texts, Power Point presentations, handouts with key answers, projects/designs of chemical experiments applying theoretical knowledge to practical level, and a test designed for verifying the improvement of the course participants. The study tools were...

Usage of Flavinium Salts as Catalysts of Oxidation Reactions Summary This diploma thesis deals with use of flavinium salts as a catalysts of oxidation reactions. 3,7,8,10-tetramethylisoalloxazin 25, 5-ethyl-3,7,8,10-tetramethylisoalloxazinium perchlorate 16 and 5-ethyl-1,3-dimethylalloxazinium perchlorate 26 had been prepared. Salts 16, 26 and other previously prepared phlavinium salt 28 were tested as potential catalysts for the oxidation of phenylboronic acids to the corresponding phenols, and especially for the use of oxygen as the oxidizing agent. The best solvent systems were found through testing and followed possible influence of the substituent on the phenyl ring of boronic acid was investigated. This initial screening can be used as a basis for further and more detailed testing of catalytic efficiency of flavinium salts and to study the oxidation mechanism.

It is the sad fact, that in the Czech Republic, consumption of fish is very low compared with the European Union average, but, at the same time, it belongs to the countries with the highest incidence of cardiovascular diseases. Sufficient intake of n-3 poly-unsaturated and highly-unsaturated fatty acids (n-3 PUFA and HUFA) is important in preventing and treating cardiovascular disease. Fish is the main source of these compounds in the human diet. This thesis examined factors influencing n-3 PUFA and HUFA levels in fish lipids and ways of increasing the n-3 fatty acid (FA) content in freshwater farmed fish.First, production of common carp (Cyprinus carpio L.) with defined flesh quality using a finishing feeding strategy and prediction of fillet FA by a dilution model were investigated, the effects of purging on lipid content and fillet quality of common carp in Central Europe were examined in fish kept for up to 70 days in clear water without feeding; and changes caused by frying on fat uptake, fatty acid composition and oxidation in the fillet of carp with increased n-3 fatty acid content were examined using four different fats to fry either plain or battered fillet.