Made available in DSpace on 2014-12-17T15:32:18Z (GMT). No. of bitstreams: 1 JoabeSP.pdf: 1318134 bytes, checksum: 051d3ab3425024319d41e93c3e0af738 (MD5) Previous issue date: 2010-03-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / T regulatory cells have the function of controlling immune responses and maintaining self-tolerance. The FoxP3 has been considered the most specific marker for Treg cells. The aiming of this paper was to evaluate the immunoexpression of FoxP3 in the inflammatory infiltrate from oral lichen planus (OLP) and to compare it with the infiltrate in fibrous inflammatory hyperplasia (FIH) and then, between reticular and erosive forms of OLP. The samples were composed by 32 cases of OLP (17 reticular and 15 erosive) beyond 10 cases of FIH that were submitted to immunohistochemistry staining for FoxP3. Localization of the staining was classified in underepithelial and intraepithelial and the amount of FoxP3+ cells was evaluated through cells counting in 10 consecutive fields, at 400x power magnification. The values were expressed in mean ? standart deviation, and submitted to statistical tests with 5% of significance level. It was observed a statistical significant difference in the amount of FoxP3+ Treg cells between the two combined forms of OLP (1,6 ? 2,2) and the FIH (0,5 ?0,4) (P<0,05). This maybe could be explained by immunological mechanism of OLP, which involves a permanent antigenic induction likely with consequent perpetuation of lesion, eliciting the proliferation and constant recruitment of Treg cells. Otherwise, FIH presents a different etiopathogenesis, in which there is also generation of a variable inflammatory infiltrate, however qualitatively distinct from that seen in OLP. The erosive form of OLP exhibited a greater number (1,7 ? 2,4) of FoxP3+ Treg cells than reticular form (1,5 ? 2,1). These alterations could have relation with the great disease activity verified in erosive OLP, or also, with abnormalities in the regulatory function of Treg cells that could cause the increase observed. Considering the capacity already well established in the literature, both about Treg cells in modulating immune responses, as in the oral mucosa in showing great potential for regeneration, it is suggested that the possibility of development and implantation of immunotherapeutic strategies that regulate the frequency and function of these cells, may help in future treatment of immune-mediated inflammatory diseases such as OLP / As c?lulas T regulat?rias (Treg) possuem a fun??o de controlar respostas imunes e manter a autotoler?ncia. O FoxP3 tem sido considerado o marcador mais espec?fico para c?lulas Treg. O objetivo deste estudo foi avaliar a imunoexpress?o do FoxP3 no infiltrado inflamat?rio do l?quen plano oral (LPO) comparado ao da hiperplasia fibrosa inflamat?ria (HFI) e posteriormente entre as formas reticular e erosiva do LPO. A amostra foi composta por 32 casos de LPO (17 reticulares e 15 erosivos) al?m de 10 casos de HFI que foram submetidos ? marca??o imunoistoqu?mica para o FoxP3. A localiza??o da marca??o foi classificada em justaepitelial ou intraepitelial e a quantidade das c?lulas FoxP3+ foi avaliada atrav?s da contagem destas em 10 campos consecutivos, com aumento de 400x. Os valores foram expressos em m?dia ? desvio-padr?o, e submetidos aos testes estat?sticos com n?vel de signific?ncia de 5%. Observou-se uma diferen?a estatisticamente significativa na quantidade de c?lulas Treg FoxP3+ entre os dois tipos de LPO reunidos (1,6 ? 2,2) e a HFI (0,5 ? 0,4) (P<0,05). Isto talvez possa ser explicado pelo mecanismo imunol?gico do LPO, que envolve uma prov?vel indu??o antig?nica permanente com conseq?ente perpetua??o da les?o, suscitando a prolifera??o e recrutamento constante das c?lulas Treg. Em contrapartida, a HFI apresenta uma etiopatogenia diferente, na qual tamb?m h? gera??o de um infiltrado inflamat?rio vari?vel, por?m qualitativamente distinto do verificado no LPO. A forma erosiva do LPO exibiu um maior n?mero (1,7 ? 2,4) de c?lulas Treg FoxP3+ que a forma reticular (1,5 ? 2,1). Estas altera??es podem ter rela??o com a maior atividade da doen?a verificada no LPO erosivo, ou ainda, com anormalidades na fun??o reguladora das c?lulas Treg que ocasionariam o aumento observado. Considerando-se a capacidade j? bem estabelecida na literatura, tanto das c?lulas Treg modularem as respostas imunol?gicas, quanto da mucosa oral em exibir um grande potencial de regenera??o, sugere-se que a possibilidade de desenvolvimento e implanta??o de estrat?gias imunoterap?uticas que regulem a freq??ncia e a fun??o destas c?lulas, possa futuramente auxiliar no tratamento de doen?as inflamat?rias mediadas imunologicamente, como o LPO

Made available in DSpace on 2014-12-17T15:32:20Z (GMT). No. of bitstreams: 1 NataliaGB_DISSERT.pdf: 3604258 bytes, checksum: d18a2353cacb2acb3240d4c04d6c8955 (MD5) Previous issue date: 2012-02-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Oral Lichen Planus (OLP) is a relatively common, cronic inflammatory disease. Its etiopathogenesis is no completely understood and several factors have proposed in attempt to explain the appearance, variety of clinical manifestations and periods of exacerbation and remission of the symptons of the lesions. The objective of the present study was to assess the presence of local factors, systemic diseases and levels of anxiety in patients with OLP, investigating their association with the clinical characteristics of the lesions. The sample consisted of 37 patients with histopathologically confirmed OLP that were submitted to a clinical exam to evaluate the presence of smoking habits, consumption of alcoholic beverages, diabetes mellitus, hypertension and hepatitis C virus (HCV) infection. The existence of skin lesions, as well as the time course, clinical form, symptomatology, number and location of the oral lesions were also registered. The trait anxiety was measured by Spielberger s Stai-Trait Anxiety Inventory (STAI), and to associate the variables we used the chi-square or Fisher s exact test. It was observed that females were the most affected (75%) and the mean age of the patients was 53,3 years. Most cases were non-smoker (97,3%) and none was drinker. Diabetes mellitus and hypertension were present in 10,8% e 16,2% of the sample, respectively and only one patient was HCV-seropositive (2,7%). Moderate levels of anxiety were seen in most cases (78,4%) and 21,6% had elevated levels. The oral lesions persisted in 95% of the cases for a period of 6 months to 13 years. The erosive form was the most prevalent (57,1%) and symptons were reported by 45,7% of the patients. Multiple lesions were frequent (60%), affecting mainly the buccal mucosa, followed by gums e tongue. There was no significant association of the presence of diabetes mellitus, hypertension and levels of anxiety with the clinical form or symptomatology of the oral lesions (p>0,005), despite a trend in patients with hypertension to have erosive lesions. It was concluded that, in the sample studied, moderate levels of anxiety were commonly observed, and the HCV infection apparently is not related to the onset of the OLP. In this study, the presence of diabetes mellitus, hypertension and levels of anxiety seem not to be associated with the clinical characteristics of the lesions of OLP / O L?quen Plano Oral (LPO) ? uma doen?a inflamat?ria cr?nica relativamente comum. Sua etiopatogenia n?o est? completamente esclarecida e diversos fatores foram propostos na tentativa de explicar o surgimento, variedade de aspectos cl?nicos e os per?odos de remiss?o e exacerba??o dos sintomas das les?es. O objetivo deste trabalho foi estudar a presen?a de fatores locais, altera??es sist?micas e n?veis de ansiedade em pacientes com LPO, investigando sua associa??o com as caracter?sticas cl?nicas das les?es. A amostra foi constitu?da por 37 pacientes com LPO confirmado histopatologicamente, os quais foram submetidos a exame cl?nico para avaliar a presen?a do tabagismo, consumo de bebidas alco?licas, diabetes mellitus, hipertens?o arterial e infec??o pelo v?rus da hepatite C (HCV). A exist?ncia de les?es cut?neas, bem como o tempo de evolu??o, padr?o cl?nico, sintomatologia, quantidade e localiza??o das les?es orais tamb?m foram registradas. O tra?o ansioso foi mensurado atrav?s do Invent?rio de Ansiedade Tra?o-Estado (IDATE) de Spielberger, e para associar as vari?veis, foram utilizados o teste qui-quadrado de Pearson ou o teste exato de Fisher. Observou-se que o g?nero feminino foi o mais afetado (75%) e a m?dia de idade dos pacientes foi 53,3 anos. A maioria dos casos eram n?o-fumantes (97,3%) e nenhum era etilista. A diabetes mellitus e a hipertens?o arterial estiveram presentes em 10,8% e 16,2% da amostra, respectivamente e apenas um paciente era HCV-soropositivo (2,7%). N?veis moderados de ansiedade foram vistos na maioria dos casos (78,4%) e 21,6% tinham n?veis elevados. As les?es orais persistiram em 95% da amostra por per?odo de 6 meses a 13 anos. A forma erosiva foi a mais prevalente (57,1%) e os sintomas foram relatados por 45,7% dos casos. Les?es m?ltiplas foram freq?entes (60%), surgindo principalmente na mucosa jugal, seguida da gengiva e l?ngua. N?o houve associa??o estatisticamente significativa da presen?a de diabetes mellitus, hipertens?o arterial e os n?veis de ansiedade com a forma cl?nica e a sintomatologia das les?es (p>0.005), apesar da tend?ncia dos pacientes hipertensos a apresentar les?es erosivas. Concluiu-se que, na amostra estudada, n?veis moderados de ansiedade foram frequentemente observados, e a infec??o pelo HCV aparentemente n?o est? relacionada ao surgimento do LPO. Neste estudo, a presen?a da diabetes mellitus, hipertens?o arterial e os n?veis de ansiedade parecem n?o estar associados ?s caracter?sticas cl?nicas das les?es de LPO