Submitted by Automação e Estatística (sst@bczm.ufrn.br) on 2018-08-03T12:06:26Z No. of bitstreams: 1 LanaSaritaDeSouzaOliveira_DISSERT.pdf: 2098247 bytes, checksum: 61e3f18e97a54dfa4dae474caf5cc5f6 (MD5) / Approved for entry into archive by clediane guedes (clediane@bczm.ufrn.br) on 2018-08-03T12:19:32Z (GMT) No. of bitstreams: 1 LanaSaritaDeSouzaOliveira_DISSERT.pdf: 2098247 bytes, checksum: 61e3f18e97a54dfa4dae474caf5cc5f6 (MD5) / Made available in DSpace on 2018-08-03T12:19:32Z (GMT). No. of bitstreams: 1 LanaSaritaDeSouzaOliveira_DISSERT.pdf: 2098247 bytes, checksum: 61e3f18e97a54dfa4dae474caf5cc5f6 (MD5) Previous issue date: 2015-08-31 / O presente estudo tem por objetivo avaliar o efeito do laser terapia combinados com arcabouço de esponja de colágeno na cicatrização de um defeito crítico na calvária de ratos. Metodologia: Foram utilizados 45 ratos Wistar, divididos em 5 grupos. O grupo G0: foi o grupo controle positivo (com defeito e sem tratamento); O grupo G1: foi o grupo tratado com esponja de colágeno; O grupo G2: foi o grupo tratado apenas com laser; O grupo G3: foi o grupo tratado com esponja de colágeno e laser; O grupo C é o grupo controle negativo: foi sem nenhum defeito ósseo e sem nenhum tratamento. O laser foi utilizado na dosagem de 120J/cm2 (potência de 50mW, tempo de exposição de 10 s), comprimento de onda de 780nm, aplicações realizadas em 4 pontos, após o procedimento e a cada 48hrs, duas vezes. Foram feitas avaliações através de raio x, lâminas histológicas e imunohistoquímica. Resultados: Os resultados histológicos e imunohistoquímicos apresentaram-se positivos para os grupos de 30 dias, com diferença significativa entre o grupo controle positivo (G0) e o grupo Colágeno mais Laser (G3). Conclusão: Observamos que a terapia de laser de baixa intensidade combinada ao arcabouço de colágeno apresentou resultados positivos para regeneração óssea no período de 30 dias. / Aim: The aim was to evaluate the effect of laser therapy combined with collagen sponge scaffold in the healing of a cranial critical defect in rats. Methods: Experimental study, prospective, in vivo. Forty five Wistar rats were divided into 5 groups. The G0 group was the positive control group ( with defective and no treatment); The group G1  with defective was treated with collagen sponge; the group G2  with defective was treated only with laser ; The G3 group with defective was the group treated with collagen and laser sponge; Group C  was the negative control group ( no bone defect and without  treatment). The laser was used at a dosage of 120J / cm2 (50 mW power, exposure time 10 sec), wavelength 780nm, applications performed on 4 points after the procedure, and every 48 h twice. The defect of 5mm was held in the skull, and after 21 and 30 days the evaluations were made by x-ray, histological slides and immunohistochemistry (RANK, OPG, TGF-β and FGF-2).  Results:  Through the radiographic findings the group had more radiopaque area was the G3 30 days. For histological analyzes the G3 30 days had more new bone formation, with less inflammatory infiltrate and large amount of osteoid. For immunohistochemical analysis were used only G3 blades 30 days and G0 30 days because there was a significant difference between these groups, and there was a higher markup OPG, indicating the reconstruction of bone tissue.  Conclusion: It was observed that the combined low-level laser therapy to the collagen scaffold showed positive results for bone regeneration within 30 days.