Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Alice Mlčochová Supervisor: PharmDr. Martina Čečková, Ph.D. Title of diploma thesis: In vitro transport of abacavir across the monolayer of Caco-2 cells; interaction with etravirine and rilpivirine. Abacavir belongs among nucleoside reverse transciptase inhibitors (NRTIs) representing a basic component of combined antiretroviral therapy used in treatment of HIV-positive patients [1]. Etravirine and rilpivirine are newer non-nucleoside reverse transcriptase inhibitors (NNRTIs) combined in cART together with NRTI. ATP-dependent transporters, so called ABC transporters, are able to affect pharmacokinetic properties of drugs, thus they are important site of drug-drug interactions affecting absorption, distribution and excretion level. P-glycoprotein (Pgp, ABCB1) and BCRP (ABCG2) belong among the most clinically important ABC transporters able to cause drug-drug interactions. The aim of this thesis was to introduce and optimize the method for evaluation of drug absorption using monolayers of Caco-2human intestine cell lines, whose integrity was verified by evaluating TEER (transepithelial electrical resistance). This model was also used for abacavir transport studies. Significant...

(english) ANALYSIS OF DRUG-RELATED PROBLEMS IN HEALTHCARE FACILITY IV. Author: Vanesa Gajdošová Supervisor: PharmDr. Martin Doseděl, Ph.D. Introduction Drug-Related Problems (DRPs) occure in lots of clinical areas.1 They are defined as pharmacotherapy related events, which actually or prospectively interfere with a therapeutic purpose.2 Aims The goal of the teoretical part of the work is to become familiar with the problem of DRPs and analyze in detail the problems of benzodiazepines and atipsychotics. The practical part of the work is focused on obtaining and evaluating of DRPs in the specific healthcare facility. Methods The data was obtained in Hamzova odborná léčebna pro děti a dospělé (Luže-Košumberk) which is the important center of rehabilitation in the Czech Republic. The audit was taking place from February 13, 2017 to February 16, 2017. The data of 94 patients from the three departments of the rehabilitation center was analyzed. The all necessary information was obtained from the medical documentation. The data was collected using the special online application and finally was statistically evaluated by the descriptive statistic. The most occurred DRPs were discussed with physicians. Results Patients used together 672 drugs (i.e. 7.15 drugs per patient). Overall, 272 DRPs were registered...

Drug interactions of drugs used in thyroid diseases Author: Veronika Teťáková1 Tutor: PharmDr. Josef Malý, Ph.D.1 1 Department of Social and Clinical Pharmacy, Charles University, Faculty of Pharmacy in Hradec Králové Introduction and objectives: Drug interactions are considered to be a significant aspect of pharmacotherapy that can lead to the potentiation of toxicity and side effects of drugs. However, their identification and adequate management of the use of drug combinations hampers a number of obstacles. The aim of this thesis was to summary information about the drug interactions of drugs used in thyroid diseases (levothyroxine, propylthiouracil, thiamazole) and to formulate articles describing the management of interactions in a clinical practice including information for the dispensation of these drugs. To compare the information presented in each database and to determine the degree of conformity between these sources. Methodology: Based on the use of numerous information sources (Micromedex, UpToDate, SPC, Stockley's Drug Interactions) lists of drug interactions of drugs used in thyroid disaeses were established. Information about these interactions were completed by findings from other sources (PubMed®, scientific reports, Google Scholar). The information presented in each of these...

Drug Information Centre service analysis I. Author: Barbora Chmelíková Supervisor: PharmDr. Kateřina Malá, Ph.D. Consultant: PharmDr. Petra Thomson Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic Introduction and aim of the thesis This study deals with the analysis of enquiries of the Drug Information Centre (DIC) of Faculty of Pharmacy in Hradec Králové, Charles University and University Hospital Hradec Králové. DIC have a unique role within provide clinical- pharmaceutical care in the provision of drug information. The aim of this study was the analysis of drug enquiries in 1994-2016 focusing on their urgency, type of enquiries and ATC groups. Methods The data analysed were collected from all recorded enquiries in the Excel database within 1994-2016. Therefore, it was necessary to transform some of the paper- recorded enquiries from 1994 to 2002 into the Excel database in which the enquiries data from 2003 to 2016 were already included. Data analysis firstly focused on the characteristics of the enquiries (quantity, healthcare professionals' characteristics such as region, position, enquiry type and urgency of the enquiries). The character of enquiries included properties and active pharmaceutical ingredient of the medicines,...

Drug Information Centre service analysis II. Author: Natália Čikovská Supervisor: PharmDr. Kateřina Malá, Ph.D. Consultant: PharmDr. Petra Thomson Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic Introduction: Drug Information Centre (DIC) of the Faculty of Pharmacy in Hradec Kralové was established in 1994 and is the only university-based DIC in the Czech Republic. DIC is providing expert information about drugs, such as answering medicines-related enquiries received from healthcare professionals. Objective: The objective of diploma thesis was to gather and analyze information about the informational service of LIC FAF UK a FN HK in 1994-2016 aimed for general characteristics of drug enquiries and professional drug information resources used for solving the enquiries. Methods: Detailed analysis of DIC activities was performed to compile suitable data. All enquiries from years between 1994 and 2016 were summarized into a database using Microsoft Excel software. General characteristics of the enquiries were analyzed, such an average number of enquiries per year or time needed for resolving one enquiry. Information about enquirers was considered as well, e.g. their profession and region, from which the enquiries were sent....

Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Consultants: Jana Kubačková, Ondřej Holas Student: Jáchym Vencl Title of Thesis: Optimization of polymeric nanoparticles separation and purification protocol Poly(lactic-co-glycolic) acid (PLGA) is one of the most successful polymeric molecule invented for biomedical use. PLGA's biggest advantage lies in its biodegradability and nontoxicityChyba! Záložka není definována.. It has been approved by EMA for human use. Because of increasing number of protein or nucleic acid based drugs the need for sophisticated drug delivery systems grows. PLGA nanoparticles (NPs) present exactly such drug delivery system capable of encapsulating large variety of compoundsChyba! Záložka není definována.. Within this study we have researched optimization of separation and purification of drug loaded NPs. They were prepared by nanoprecipitation of PLGAChyba! Záložka není definována.. Separation and purification of NPs was done using multiple cycles of centrifugation. We evaluated purification of particles prepared from five different PLGA polymers. Different centrifugation times were applied to find the most effective way. Water and two types of stabilizers each one in two concentrations have been used as purification...

Drug Information Centre service analysis III. Author: Tereza Harazimová Supervisor: PharmDr. Kateřina Malá, Ph.D. Consultant: PharmDr. Petra Rozsívalová Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic Introduction: Drug Information Center (DIC) of the Faculty of Pharmacy in Hradec Králové, Charles University, and University Hospital Hradec Králové was established in 1994. Major activity of the DIC is providing expert information about drugs - primarily in the form of answers to various medicines-related enquiries sent by healthcare professionals. DIC deals with enquiries related to general pharmacotherapy including complementary and alternative medicine (CAM). Aim of study: Aim of the study was to evaluate DIC's activity in the period 2015-2017 through feedback from questioners and to analyse enquiries related to CAM in the period 1994-2017. Methods: The feedback of questioners whosent the enquiry to DIC from 2015 to 2017 was obtained using an online questionnaire containing 18 items - sociodemographic data and awareness of DIC, quality of answers to enquiries, satisfaction with provided services and lastly resolving of CAM related issues in practice. Furthermore, analysis of DIC's database in the period 1994-2017 was performed....

Title of thesis: Analysis of drug-related problems in a healthcare facility VI. Candidate: Kristína Záleská Supervisor: PharmDr. Martin Doseděl, PhD. Department: Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University Background and aim: A drug-related problem (DRP) has been defined as an event or circumstance involving drug therapy that actually or potentially interferes with desired health outcomes [1]. The theoretical background chapter is aimed to give a detailed description of selected studies dealing with drug-related problems in various settings. The retrospective cross- sectional study, described in the experimental part of the thesis, was carried out in order to identify, classify and assess DRPs in a rehabilitation facility and to discuss the results with a physician and subsequently determine intervention acceptance rate. Methods: The data were collected during a nine-day-long audit in three wards of a rehabilitation facility in the Czech Republic through a review of medical documentation. The detected DRPs were classified using the modified version of PCNE classification v5.01 and their severity was assessed on a scale of 0 to 5. The DRPs were subsequently discussed with physicians and the pharmaceutical intervention acceptance rate was...

Analysis of drug-related problems in a health facility IV. Author: Linda Šubrtová Tutor: PharmDr. Josef Malý, Ph.D. Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University Introduction: Increasing use of drugs leads to a growing risk of occurence of drug-related problems. These problems can often endanger patient's health and increase the economic cost of the treatment. However, many of these problems can be prevented by controls and collaboration of all health care subjects. Objective: The aim of this work was to carry out a revision of pharmacotherapy in a rehabilitation health facility and to analyse acquired drug-related problems. Methodology: The collection of data took place from 13 to 15 February 2017 in health facility in the Czech Republic. The review of pharmacotherapy was performed by a three-member team (clinical pharmacist, pharmacist, work researcher). The data obtained from the provided medical records were recorded into the database, which was prepared for the purpose of the pharmacotherapy review. Recorded were: the patient's age and sex, allergic, family, work, social and personal history, physical and laboratory examinations and complete pharmacotherapy, which included over-the-counter drugs and dietary supplements, including dosage...

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and toxikology Student: Zuzana Matiašková Supervisor: doc. PharmDr. Martina Čečková, Ph.D. Title of diploma thesis: Role of selected ABC and SLC transporters in transmembrane permeability of maraviroc: effect on transport in placenta Antiretroviral drug maraviroc is an inhibitor of CCR5-trophic HIV virus and belongs to the group of entry inhibitors. Nowadays, maraviroc is administered as part of combination antiretroviral therapy (cART) primarily in adults, children over the age of two and pregnant women to reduce the risk of transmission of HIV to the fetus. The knowledge of interactions of maraviroc with drug transporters in placenta is crucial for optimizing the therapy during pregnancy, both in terms of efficacy and potential adverse effects. Maraviroc is known substrate of ABCB1 transporter, which plays a protective role to the fetus by its efflux activity in the apical membrane of trophoblast. However, the results of recent study employing dually perfused human placenta suggest involvement of other transport mechanisms in the maraviroc transplacental pharmaocokinetics, especially those operating in the opposite direction to ABCB1. The aim of this study was to evaluate in vitro studies whether, besides ABCB1,...