The aim of this thesis is to incorporate the smallest organisms, in particular the heterotrophic bacteria and their predators, more fully into current descriptions of aquatic community structure and dynamics. A strong, positive empirical relationship was found between bacterial abundance and chlorophyll concentration in freshwater and marine systems. Common members of the photosynthetic phytoplankton (all chrysophyceans) were shown to be major, even dominant, grazers of these bacteria. This phagotrophic capability is quantitatively important to the bacterioplankton, to the mixotrophs themselves, and in some cases, to the structure of the limnological community as a whole. Grazing by mixo- and heterotrophic protozoans is concentrated on the largest bacterial cells that also have the greatest growth rates. Relatively lower removal rates of the tiniest cells, with low growth rates, is proposed to explain their numerical dominance in lakes.

Thermal structure in the seasonal thermocline at Lake Tahoe was investigated through the analysis of vertical temperature profiles taken in the upper 70 m during late summer stratification. Different ranges of horizontal and temporal scales were sampled in each of three subsets of finestructure profiles: (1) a three-week sequence at a midlake station, (2) a 2 1/2 hour, 12 km transect across the lake, and (3) a 2 1/2 hour sequence of intensive sampling over a 200 m transect line at midlake. The ensemble mean profile was much the same for each set, but the ensemble temperature variance, similar for (1) and (2) , was smaller at all depths and distributed differently in the vertical for (3). As a working hypothesis, it is assumed that a substantial amount of the observed temperature variability is due to internal wave displacements. Within this framework, the observations of set (3) are found to be consistent with generation by a field of small-scale internal waves obeying WKB displacement scaling. In contrast, over the longer time scales of (1) , or the larger area of (2), the dominant contribution to the variance appears to have come from a few, low order, vertical modes. Vertical wavenumber spectra of temperature fluctuations for all three sets were similar for wavenumbers greater than about 0.1 cpm, falling as wavenumber to the -5/2 power. When interpreted as spectra of vertical displacements, the level in the 0.1 to 1.0 cpm decade was fairly constant even though the local buoyancy frequency for individual records varied from 3 to 13 cph; this spectral level was somewhat lower (by a factor of 0.3 to 0.7) than that reported for various oceanic results. WKB internal wave scaling did not improve the spectral collapse effected by the "displacement scaling" of temperature spectra. Horizontal-temporal coherence fell to 0.5, for vertical wavelengths of 13 m, at a separation of 70 in and 12 minutes. For 6.5 m wavelengths the separation was 44 in and 7 minutes. A set of microstructure profiles was also obtained during the same project. These revealed the characteristic patchiness of microstructure activity, and the occurrence of the most intense signals in a shore-bound mixing layer over the steeply sloped bottom. With two horizontally separated thermistors on one instrument, it was determined that the shape of microscale features varied with scale, larger features being more flattened. The shape also depended on the local stratification. / Graduation date: 1980

The aim of this study is to compare observational data to data simulated by a one dimensional model. Observational data collected from January to July 2006 at Lake Whitney, Texas, included water current velocities from an Acoustic Doppler Current Profiler, and an Acoustic Doppler Velocimeter from which shear stress, turbulent kinetic energy dissipation rates, and turbulence kinetic energy were computed using several methods. Numerical model experiments, forced by the surface heat and momentum fluxes, velocity profiles, and temperature profiles were conducted to simulate the development of the turbulence parameters. Two equation models, k-epsilon and k-kl, were used to find which model best describes the observed physical processes (turbulence kinetic energy, turbulent kinetic energy dissipation rate and velocity variances). The combined observational and simulated results show a change in stratification levels that consequently leads to variations in turbulent kinetic energy dissipation rate, turbulent kinetic energy, and the velocity variances. In order to investigate the accuracy of the model, we quantitatively compared these parameters to estimates from the observed data in the bottom boundary layer. In general, the model and observational data agree well for the three parameters, with the exception of some time periods, during which the model prediction differed from the observed. This was at times when the Acoustic Doppler Velocimeter measurements were at the noise level of the instrument. Overall, the k-kl model simulation results appear to be closer to the observational results during the weakly and strongly stratified periods than the k-epsilon model.

This research focused on features of a genetically isolated population of cutthroat trout (Oncorhynchus clarki) in the Triangle Lake basin of coastal Oregon. A falls at the outlet of Triangle Lake has blocked upstream migration of trout and anadromous salmonids into the basin. Cutthroat trout were found throughout the six study streams of the Triangle Lake basin in association with other native fishes and introduced warmwater fishes. Warmwater species (e.g. Centrarchidae) were restricted largely to the lakes of Triangle Lake basin, and did not comprise a significant part of the stream fish fauna. Salmonids and cottids dominated the upper forested reaches of the basin, whereas nonsalmonids dominated the lower reaches. The middle reaches contained a transitional fish community between the upper and lower reaches. Reach type was a major factor influencing cutthroat trout density and size distribution. Areal densities of cutthroat trout were highest in the upper reaches and lowest in the lower reaches with the exception of 0+ cutthroat trout, which occurred at similar densities in all reaches. The highest frequency of 1+ cutthroat trout occurred in the middle reaches, whereas the highest frequency of 0+ cutthroat occurred in the lower reaches. Within reaches, channel unit type influenced the density and age structure of cutthroat trout. Pools and rapids had the highest densities of cutthroat trout, whereas riffles, glides, and cascades had lower densities. Larger trout were found primarily in pools and rapids. Planted steelhead fry (Oncorhynchus mykiss) were found in two streams, Congdon Creek and Lake Creek. Although most steelhead fry leave the basin as smolts, some steelhead appear to become resident and may hybridize with native cutthroat trout. Cutthroat trout spawning was observed from late December 1987 through late May 1988. Differences in the time and place of spawning may serve to genetically isolate two populations of cutthroat trout in Triangle Lake basin. One population may be lake dwelling as adults and spawn lower in the basin in late spring. The other population may be stream dwelling and spawn in the winter during higher flows, which allows them to spawn in the upper reaches of the basin. / Graduation date: 1994

Since the 'discovery' of the high abundance of viruses in aquatic environments, it has been generally assumed that viruses in lakes are similar to those in oceans. I directly compared these two systems using a large, robust data set. Viral abundance was significantly different among the surveyed environments. The relationship between viral and bacterial abundance indicated a fundamental difference between lakes and oceans, and suggested that viruses infecting phytoplankton may be more important in lakes. Molecular techniques (PCR & DGGE) were used to document spatial and temporal variations in the richness of viruses that infect eukaryotic phytoplankton (Phycodnaviridae) in lakes at the Experimental Lakes Area (ELA). Phycodnavirus richness was highest in the eutrophic lake, and during the spring/early summer in all the lakes. Viral richness was closely associated with phytoplankton abundance and composition. As a result, richness was influenced by trophic status, while patterns of richness were affected by regional climatic conditions. Phylogenetic analysis of environmental Phycodnavirus DNA polymerase (pol) sequences indicated that freshwater Phycodnaviruses are genetically different from cultured isolates and marine environmental sequences. A genetic distance analysis indicated that pol sequences > 7 % different infected different host species. Therefore, the 20 different freshwater sequences likely infected nine different hosts. Multivariate statistics identified seven possible phytoplankton hosts, including chlorophytes, chrysophytes, diatoms and dinoflagellates. Finally, the modified dilution experiment was evaluated as an approach for estimating viral-mediated phytoplankton mortality in two lakes at the ELA. Experiments resulted in non-significant apparent growth rate regressions. While a model analysis, indicated that the method was sensitive to poorly constrained parameters such as burst size and length of the lytic cycle, making it unsuitable for estimating mortality rates in these lakes. These studies indicate that Phycodnaviridae are a genetically rich and dynamic component of lakes. Their richness is influenced by both the chemical and physical components of their environment. Although the presence of these viruses indicates that they are a source of phytoplankton mortality, the magnitude of their impact on structuring phytoplankton communities awaits methodological advances. Nonetheless, these findings support the view that viruses infecting phytoplankton are ecologically important components of lake ecosystems.

Advancements in technology in mining have allowed previously unfeasible ore bodies to be developed. Paste backfill is one technological advancement that has allowed for the development of high-grade, low tonne production when employing the cut and fill mining method. Goldcorp Inc.'s Red Lake Mine currently utilizes this method and is the site for the study of this thesis. Paste backfill (paste) is defined as a mine backfill material that consists of eighty-five percent solids by weight and does not bleed water when placed often consisting of between two and fifteen percent Portland cement by weight. A paste barricade or paste fill fence is a constructed barricade whose purpose is to retain backfill within a mined out stope. The construction of the barricade varies with different operations, for Red Lake Mine the barricade consists of an anchored rebar skeleton covered with an adequate thickness of shotcrete. The majority of the applicable barricade research focuses on hydraulic fill barricades in open stope mining. The barricade pressures in these instances are much larger than those experienced in paste backfill barricades. As such, the current paste loading theory is based on material with a different loading mechanism. Although some research is currently underway, the majority of the barricade research is based on brick barricades and not the shotcrete, rebar skeleton as used at Red Lake. Catastrophic failures of barricades can occur without an understanding of the loading mechanisms. Based on the catastrophic risk, this thesis provides an investigation into the behaviour of the paste backfill and paste barricades at Red Lake Mine in order to provide a safe, cost effective design of paste barricades. This thesis develops an understanding of paste loading mechanisms and barricade capacity derived from a field study of nine instrumented fill fences at Red Lake Mine. Eight of thefences were instrumented to monitor the reaction strain in the fence and the applied pressures during standard production paste pours, the ninth fence was a controlled destructive test that determined the ultimate capacity of the fence. The data for these tests were gathered in real time and was subsequently reduced to assist in analysis. Yield Line Theory, Rankine Theory, strain induced stress, stress vs. strain analysis and numerical modeling were used to develop an understanding of the paste loading mechanisms and the capacity of the paste fill barricades. The analysis determined that the paste backfill behaves as a Rankine-like soil in the initial stages of loading with an average coefficient of lateral earth pressure, Ka, of 0.56. The destructive test determined that the yielding stress of a paste barricade is approximately 100 kPa. Further findings from the research determined that the rate of placement of paste does effect the loads applied to the fence and that the largest pressures exerted on the fill fence occur when paste lines were flushed with water at the end of the pour. This thesis provides an understanding of the paste loading and fill fence interaction with respect to failure. Based on this research the Red Lake Mine should be able to increase production without increasing risk to mine personnel by quantifying the overall loading and strengths of the fill barricade.

The requirements of ecological studies and water resource management plans are driving demand for hydrological models of the rivers of the arid zone. Knowledge of the hydrology of Australia’s arid zone is poor, yet is critical in understanding the ecology of the region. The research presented in this thesis seeks to address some shortcomings in our understanding of the hydrology of the Australian arid zone. In particular, the research examines the requirements for modelling the flow regime of arid zone rivers, concentrating on the rivers of the Lake Eyre Basin (LEB). The LEB has exceptionally low annual runoff of 3.5 mm, its major rivers develop over extremely low gradients and are characterised by very wide floodplains and complex anastomosing flow paths in their mid to lower reaches. This research was driven by both a practical and theoretical impetus. Practically, hydrological data were required at the water body scale for a large number of sites across three river systems of the LEB, for use in a study, known as ARIDFLO, of the ecological responses to hydrological conditions. Because of the remoteness of these sites and the paucity of gauging stations on these rivers, modelling of the rivers was the only method for delivering the required discharge data. Theoretically, the challenge was set for creating hydrological models for some extraordinarily complex river systems, in terms of their size, catchment characteristics and flow regime variability. (For complete abstract open document)

Mountain precipitation, as a major component of global ecology and culture, requires diverse observation-based distribution studies to improve process characterisation and so enhance environmental management and understanding. Analysis of data from an array of precipitation gauges within the nationally important, and internationally extreme, mountainous Lake Pukaki catchment in New Zealand has been undertaken in an effort to provide such a study, while also improving local hydrological understanding. An objective observation based undercatch-corrected 1971-2000 average annual precipitation distribution has been prepared for the mountainous Lake Pukaki catchment, New Zealand. Precipitation records from 58 gauges at 51 sites, augmented with 10 new gauges, were used in preparation of the distribution. The assessed undercatch correction of 17 % across the catchment indicates that mountain hydrological investigations in New Zealand that use precipitation data and yet do not consider undercatch will be in considerable error. The average annual distribution confirms the existence of high precipitation magnitudes and horizontal gradients in the catchment in comparison with other mountain regions around the world. The high magnitude is unusual when its position in the lee of the principal orographic divide is considered indicating rare precipitation distribution processes occur in the region. Consideration of river flows, glacial change and evaporation led to a confirmation of the gauge derived average catchment precipitation. Precipitation to wind direction relationships identified the predominant westerly wind to be the primary precipitation generating direction with large magnitude events biased towards the northerly direction. All directions from the eastern side of the mountain divide had the lowest frequency and daily precipitation magnitude. Derivation of wind-classed precipitation distributions identified a distinctive south east to north west precipitation gradient for all wind directions, most severe for the north west direction and least severe for the easterly direction. Precipitation extent was greatest for the northerly direction and least for the south south westerly. The wind-classed distributions enable the estimation of daily precipitation likelihood and magnitude at any location in the catchment based on knowledge of the synoptic wind flow direction and precipitation at just one reference site. Improved river flow and lake inflow estimates resulted from the use of wind classed daily precipitation estimates validating the quality of the wind classed distributions. From 1939 to 2000 there has been no statistically significant trend in precipitation magnitudes, frequencies, or extremes in the catchment. At Aoraki/Mt Cook village, in the upper catchment, there have been significant increases in magnitude, frequency and extremes associated with the phase change of the Interdecadal Pacific Oscillation (IPO) in 1978. This change can be explained by the increase in strength of westerly winds for the different IPO phases but not by a change in frequency of different wind directions. In the lower catchment the IPO relationship is of an opposite sense to that observed in the upper catchment, indicating that the areas operate under two different climate regimes with different precipitation controls. The significant relationship to the IPO phase indicates that it is more important than climate warming in terms of future precipitation distribution in the Lake Pukaki catchment, and by extension the Southern Alps. The distributions prepared provide a valuable tool for operational and academic hydrological applications in the region. In addition, they provide a valuable characterisation of the precipitation in a Southern Hemisphere mid-latitude lee to predominant westerlies glacierized mountain catchment. From this standpoint they highlight the contrast to Northern Hemisphere mountain precipitation distributions commonly used in model validation studies, thereby providing an extension of locations with which to refine orographic precipitation process understanding.

Thesis (M.S. - Watershed Management)--University of Arizona. / Includes bibliographical references.

Thesis (M.S. - Hydrology and Water Resources)--University of Arizona, 1982. / Includes bibliographical references (leaves 76-79).