As limited prey availability and persecution by humans in response to livestock predation are key conservation concerns for the Cape leopard (Martins & Martins 2006), the present study aimed to provide more information regarding their feeding habits. The first objective was to determine whether the Cape leopard was subject to a change in their prey base and how they responded to the change. This was established by comparison of their current diet in the Cederberg and Gamka Mountains, determined by using scat analysis techniques, with a previous dietary assessment (Norton et al. 1986). The second aim was to provide a preliminary assessment of the prey preference of the Cape leopard and examine the utility of camera trap surveys to determine leopard prey preference. Leopard diet in the Cederberg and Gamka Mountains consisted largely of small- (1-10 kg) and medium-sized (10-40 kg) mammals; rock hyraxes (Procavia capensis) and klipspringers (Oreotragus oreotragus) were key prey items. In terms of regional variation in leopard diet, there was a significant difference in the average weight of prey utilized in the Cederberg and Gamka Mountains. Despite the importance of prey availability of suitable size, their flexibility in terms of prey size utilization reflected their ability to switch to smaller prey to fulfil their dietary requirements, when prey is limited. The study suggested a dietary shift, with significant variation in prey species utilization in both regions. The shift did not appear to be in response to prey scarcity, but rather a reduction in key prey species, particularly the rock hyrax. The shift involved an increase in the number of species utilized, and only a very small increase in livestock predation in both areas. There was however no significant variation in prey size category utilization. This demonstrated their dietary flexibility, as well as the importance of suitable prey sizes rather than the presence of specific prey species to fulfil their dietary requirements. The camera trap survey revealed a strong correlation between the number of camera trap days and the number of photographs taken of identifiable species. Variation of this correlation between different habitats supported the notion that individual images are a better unit to determine sampling efficiency than trap days. The camera trap survey also showed that small rodent availability was underestimated by camera trap surveillance, which resulted in poor prey preference estimation. It was therefore suggested that camera trap surveys be restricted to the surveillance of larger prey species (> 1 kg). By excluding small rodents from the analysis, prey preference could be estimated for other species and prey size categories. Small- and medium-sized mammals were significantly preferred, whereas large mammals were significantly avoided by the Cape leopard.

M.Sc. (Zoology) / The reproductive biology of the South African leopard, Panthera pardus has not been studied in detail. In South Africa little is known about the population numbers of leopards due to their solitary and nocturnal nature and currently the conservation and management of leopard populations relies mainly on the contributions of non-governmental organisations, academic institutions and private individuals. The aim of this study was to provide baseline information for the development of in-situ and ex-situ reproductive conservation methods for the leopard. In order to meet this aim, the following objectives were established: 1) determine the degree of relatedness of the leopards sampled, 2) establish baseline parameter values of a whole blood count and describe the ultrastructure of the blood cells, 3) obtain semen by means of electroejaculation and determine the efficiency of a previously described cryopreservation protocol for leopard spermatozoa, 4) describe the morphology and ultrastructure of the leopard spermatozoa using florescence and electron microscopy, 5) describe the histology and ultrastructure of the leopard testes and the events of spermatogenesis using light and electron microscopy. Between January 2011 and February 2013, blood and semen samples were obtained from eleven leopards after being sedated with a combination of Medetomidine and Ketamine. The DNA was extracted from the blood (ARC Genetics Department) and analysed (Onderstepoort Veterinary Genetics Laboratory). The blood was also used for the analysis of the baseline blood parameter values (Lancet Laboratories). Whole blood was fixed in 2.5% phosphate buffered gluteraldehyde and prepared for transmission and scanning electron microscopy to describe the ultrastructure of the cells. Techniques to examine sperm morphology included florescence and electron xviii microscopy. The semen was fixed in 2.5% gluteraldehyde and phosphate buffer for the ultrastructural assessment. Testes samples obtained from a leopard that died during transportation were fixed in Bouin’s fixative and a phosphate buffered 2.5% gluteraldehyde solution for light and electron microscopy respectively. The testes samples were prepared using standard techniques and stained with Hemotoxylin and Eosin for light microscopy and uranyl acetate and lead citrate for electron microscopy. The DNA analysis revealed that two pairs of leopards were related on a half-sibling level. The mean parameter values of the whole blood count of P. pardus were similar to the values recorded for Asian leopards, P. pardus African lions, Panthera leo and bobcats, Lynx rufus and fell within the normal ranges for the domestic cat, Felis catus. The ultrastructural assessment of the blood cells was comparable with those that have been described for the Asian leopard as well as most other mammalian species. A small volume of semen (≤0.5 ml) could be obtained from five out of nine male leopards that were sampled. The morphology and ultrastructure of the leopard spermatozoa conforms to the generalised structure of spermatozoa of most mammalian species. A large number of morphologically abnormal spermatozoa were noted. This has also been reported for many feline species, including the Indian leopards. Spermatozoa abnormalities identified included coiled tails, cytoplasmic droplets and knobbed acrosomes. The cryopreservation of the spermatozoa yielded a maximum post-thaw progressive motility of 24.4%. The histology and ultrastructural events of spermatogenesis in the leopard testes were compared to that of the domestic cat and some differences were observed between the domestic cat testes and leopard testes. The results of this study provide baseline information on the genetic diversity and reproductive biology of the leopards in South Africa. This can be used in the development of assisted reproductive techniques that may one day aid in conservation strategies for the leopards.

Leopards (Panthera pardus) are the most common large predators, free roaming outside of protected areas across most of South Africa. Leopard persistence is attributed to their tolerance of rugged terrain that is subject to less development pressure, as well as their cryptic behaviour. Nevertheless, existing leopard populations are threatened indirectly by ongoing transformation of natural habitat and directly through hunting and conflict with livestock farmers. Together these threats may further isolate leopards to fragmented areas of core natural habitat. I studied leopard habitat preferences, population density, diet and the attitudes of landowners towards leopards in the Little Karoo, Western Cape, South Africa, an area of mixed land-use that contains elements of three overlapping global biodiversity hotspots. Data were gathered between 2010 and 2012 using camera traps set up at 141 sites over an area of ~3100km², GPS tracking collars fitted to three male leopards, scat samples (n=76), interviews with landowners (n=53) analysed in combination with geographical information system (GIS) layers. My results reveal that leopards preferred rugged, mountainous terrain of intermediate elevation, avoiding low-lying, open areas where human disturbance was generally greater. Despite relatively un-fragmented habitat within my study area, the leopard population density (0.75 leopards/100km²) was one of the lowest yet recorded in South Africa. This may reflect low prey densities in mountain refuges in addition to historical human persecution in the area. Currently local landowners are more tolerant of leopards than other wildlife species with incidents of conflict involving leopards being rare relative to black-backed jackals (Canis mesomelas), baboons (Papio hamadryas), caracals (Caracal caracal) and porcupine (Hystrix africaeaustralis). Although current levels of conflict between leopards and stock farmers are low, leopards do depredate livestock, which constitute 10-15% of their diet. Improved livestock husbandry measures and co-operation between conservation authorities and farmers are necessary to mitigate such conflict and balance economic security with biodiversity conservation in the region. Leopards are the only remaining top predators throughout much of the Little Karoo and the Western Cape and as such are predicted to play a critical role in ecosystem structure and the survival of other species. Current high levels of connectivity between areas of suitable leopard habitat bode well for the conservation status of leopards within this region and future conservation efforts need to ensure that narrow corridors linking such habitat are preserved. The potential for leopards to serve as both an umbrella and a flagship species for biodiversity conservation suggests that long term monitoring of this population would be a conservation priority for the Little Karoo.

The conservation of leopards in the Eastern Cape Province requires a holistic approach that considers both predator-human interactions as well as the biology of the carnivore. Numerous studies have been conducted on leopards within protected areas; however more information regarding the species is needed outside these areas to facilitate effective management of predators. The spatial ecology of the leopard (Panthera pardus) were studied in the Baviaanskloof and GAENP in the Eastern Cape. The Baviaanskloof is an extensive area of mountainous terrain (approximately 2665km²) which has a mosaic of land uses, and leopards move from conservation areas to farmland where they come into contact and conflict with farmers. This study examined the spatial ecology of leopards living on farmlands adjacent to protected land. The space utilization and activity patterns of six leopards were analysed. These animals were caught and released on farmlands in the Baviaanskloof (n=4) or translocated (n=2) when not possible to release on site. The animals were caught by means of fall-door, walk-in traps and fitted with Vectronic GPS collars that facilitated the collection of high quality GPS fixes from each animal. Data was collected using VHF and UHF telemetry to download data. An understanding of spatial requirements in areas with different land use, and the extent of overlap of space use with other leopards allow, for the first time in the region, the calculation of possible maximum population size. Analysis of range size was carried out using two methods: minimum convex polygon, and Kernel Utilization Distribution. Finally, a key predictor of space use is prey availability. I assessed the prey base using a grid of camera traps. The studied leopards revealed large range utilization with minimal overlap. The activity patterns suggest there is no preference between diurnal and nocturnal activity patterns and the population density was estimated between 0.3 - 1.3 leopards per 100km². Large home range sizes and low population densities suggest that leopards require large areas of suitable habitat, and that conservation efforts need to be extended beyond protected areas to ensure the long-term viability of leopard populations in such areas.