It is the purpose of this investigation to determine the variation of glyoxalase activity in liver of normal mice and in the liver of mice bearing a lymphosarcoma and to compare the glyoxalase activity of the lymphosarcoma with values previously reported in the literature for other tumor types. Further, if there is indeed a variation in liver glyoxalase activity between normal and tumor-bearing mice, it will be compared to the variation in the activity of two other enzymes present in liver tissue in relatively high concentration.

The purpose of this investigation was (1) to isolate and purify glyoxalase I from the livers of normal DBA/lJ mice and the livers from mice bearing a lymphosarcoma tumor; and (2) to determine, at least with respect to glyoxalase I, if the tumor has an effect on the chemical properties or structure of macromolecules in an organ removed from tumor locale and not histologically affected by its presence.