Orientador: Raquel Gonçalves / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agricola / Made available in DSpace on 2018-08-04T02:41:40Z (GMT). No. of bitstreams: 1 Neri_AntonioCarlos_D.pdf: 2011024 bytes, checksum: f186a3983118cb93726e980979e74b80 (MD5) Previous issue date: 2003 / Resumo: As madeiras de reflorestamento têm grande importância na economia nacional, movimentando importantes setores, tais como os de papel e celulose, de recursos energéticos, moveleiros e de construção civil. Embora o Brasil apresente um grande potencial florestal, sendo detentor das maiores reservas florestais tropicais do mundo e possuindo condições de clima e solo favoráveis à implantação de florestas de rápido crescimento, sua participação no comércio internacional de madeiras (2,1%) é, ainda, muito pequena. Em geral, grande parte das indústrias que processam madeiras apresenta baixo rendimento de madeira serrada e qualidade insuficiente do produto final, principalmente devido ao emprego de técnicas de processamento (conhecimento de parâmetros de corte) além de fatores de planejamento da serraria (implantação e Layout) inadequadas. Esse fato revela, portanto, que o setor madeireiro necessita incorporação de inovações tecnológicas. A caracterização de espécies de reflorestamento, no que diz respeito aos parâmetros de usinagem, tais como forças de corte, geometria adequada da ferramenta, espessura de corte, densidade, etc., é imprescindível no processamento da madeira. O objetivo principal deste trabalho foi a determinação dos principais parâmetros de corte (forças de corte, pressão específica de corte e ângulo ideal de saída da ferramenta) para a espécie Pinus taeda, mediante o estudo do comportamento das forças de corte em diferentes posições e regiões de madeira juvenil e adulta do tronco. Além desse objetivo, o trabalho pretendeu, também, avaliar a metodologia de determinação de forças de corte para as espécies de eucalipto (Citriodora, Saligna e Grandis), proposta pelo autor em trabalho anterior. Para alcançar esses objetivos, foram ensaiados 96 corpos-de-prova da espécie Pinus taeda, obtidos de seis árvores provenientes do horto florestal de Manduri, S.P, com um total de 5760 ensaios. Como resultado, para o Pinus taeda foram determinadas as forças em função dos parâmetros espessura de corte e ângulo de saída, bem como foi calculado o ângulo de saída x ideal, que resultou da ordem de 40° para pequenas espessuras de corte e da ordem de 30° para maiores espessuras. Para as espécies de eucalipto (Citriodora, Saligna e Grandis), cujos resultados experimentais foram obtidos pelo autor em trabalho anterior, bem como para o Pinus taeda, foi calculada a pressão específica de corte. Os resultados permitiram, ainda, comprovar que a metodologia avaliada é adequada para utilização na espécie estudada / Abstract: The reforestation wood has great importance in the national economy, running important sectors, such as paper and the cellulose, energy resources, furniture and civil construction. Although Brazil presents a great forest potential, holding the biggest forest reserves of the world and possessing soil and climate conditions favorable to the implantation of forests of fast growth, its participation in the wood international trade (2.1%) is, still, very small. The techniques currently used in processing the wood, usually do not provide good income, neither propitiate good quality in the final product as well. Mainly due to the usage of inadequate parameters of the cutting process, along with inappropriate sawmill planning (implantation and Layout). This fact discloses, therefore, that the lumber sector needs the incorporation of technological innovations. The characterization of kinds of reforestation that respect the cutting parameters, such as, cutting force, adequate geometry of the tool, cutting thickness, density, etc., is essential in the processing of the wood. The main objective of this work was the determination of the main parameters of cutting (cutting force, specific cutting force and ideal exit angle of the tool), for the species of loblolly pine (Pinus taeda L.) by the study of the behavior of the cutting force in different positions of the trunk and in different regions of juvenile and adult of wood. Beyond this objective, the work is intended, also, to evaluate the methodology of determination of cutting force for the wood of eucalyptus, proposed by the author in previously work. To reach these objectives, 96 specimens of the specie Pinus taeda were assayed, taken from 6 trees proceeding from a research forest of Manduri, S.P, resulting in 5760 tests. As a result, the force as function of the evaluated parameters had been determined for Pinus taeda, as well, the ideal cutting angle for specimens thickness environ 0.2 mm was near 40° and for thickness between 0.4 mm and 1.0 mm was near 30°. The specific cutting force was calculated for Pinus taeda, as well as for the Eucaliptus. The former with experimental results obtained in this work and the latter using experimental results obtained in previous work from the author. Finally, the results had proved also that the methodology adopted in this research is appropriated to use in the studied species / Doutorado / Construções Rurais / Doutor em Engenharia Agrícola

Loblolly pine (Pinus taeda) and coppiced sweetgum (Liquidambar styraciflua) seedlings were grown in competition with a native weed community using soil and seed bank collected near Appomattox, Virginia. Seedlings and weeds were exposed to CO₂ (ambient and elevated) and water (water stressed and well watered) treatments for approximately one growing season in closed top chambers. Weed growth had an effect on tree growth, but the amount of variation in tree biomass explained by weed biomass was very low. It appears that the tree seedlings benefited more from available resources than the herbaceous weeds. The influence of competition with loblolly pine and elevated CO₂ did not have an influence on total weed biomass; however, it did favor C3 weed community development regardless of water availability. This suggests that weed community composition may shift toward C₃ plants in a future elevated CO₂ atmosphere. Loblolly pine height, diameter, needle, shoot and total biomass were significantly greater in the well watered treatment than the water stressed treatment. Pine root, needle, shoot and total biomass were significantly greater in the elevated treatment than the ambient treatment. While not significant, root biomass of water stressed pine seedlings was 63% greater in the elevated CO₂ treatment than the ambient treatment. There was a significant water and CO₂ interaction for pine root:shoot ratio. Under elevated CO₂, root:shoot ratio was significantly greater in the water stressed treatment than the well watered treatment. In contrast, root:shoot ratio in the ambient treatment was nearly identical under both water treatments. These results indicate that loblolly pine will respond favorably in an elevated CO₂ atmosphere, even under dry conditions. The coppiced sweetgum seedlings responded favorably to well watered conditions with significant increases in leaf area, specific leaf area, leaf, shoot and total biomass compared to water stressed conditions. Leaf, root, shoot+stump and total biomass of sweetgum significantly increased and specific leaf area decreased under elevated CO₂ compared to ambient CO₂, but differences were smaller than previous findings. This indicates that coppicing may dampen the growth response to elevated CO₂, at least in the initial growth stage after coppicing. / Master of Science

Managed loblolly pine (Pinus taeda) stands are common in the southeastern United States and provide young forest conditions for early successional breeding birds. Common pine management practices (e.g. hardwood control) and novel practices (e.g. switchgrass [Panicum virgatum] intercropping), may influence breeding bird use. I evaluated breeding bird abundance, diversity, and resource use within loblolly stands treated to control hardwood species, intercropped with switchgrass, and with no additional management. Hardwood control and switchgrass intercropping both decreased hardwoods and shrubs, and increased forbs. Switchgrass intercropping increased some early successional bird abundances. However, diversity was not different among treatments. Switchgrass intercropping increased 13C:12C in birds and vegetation; both hardwood control and switchgrass intercropping increased 15N:14N in birds and vegetation. Birds moved freely among stands of different ages and management practices. My results suggest these practices improve conditions for some early successional birds and may help maintain ecological value of loblolly stands for birds.

Woody competition during mid-rotation limits available nutrients to crop species. Mid-rotation competition control was evaluated to determine if applications would result in significant increase in growth. Two studies were initiated using herbicides and prescribed burning on mid-rotation loblolly pine plantations. The first study contained afforested stands in Mississippi. Combination of imazapyr and burn was applied. The second study contained reforested sites in Mississippi. Treatments consisted of imazapyr and burn, imazapyr only, burn only, and a control. Five-year post-treatment measurements for the first study showed no significant treatment differences in height, diameter, basal area, or volume growth. Nine-year post-treatment measurements for the second study showed no significant differences in growth using the same measurements. Although previous research has documented significant growth responses to mid-rotation competition control, results from this study demonstrate that increased growth does not always result from mid-rotation competition control, suggesting initial site conditions may dictate degree of response.

Increased growth rates, wood quality, and disease resistance have been accomplished within loblolly pine (Pinus taeda L.) through genetic selection and improved management practices. Genetic engineering of trees has the potential to further improve these selections but also needs to be tested. Two studies were conducted. Study one compares three levels of genetic improvement: Mass-Control Pollinated (MCP), Second Generation Op (2nd gen), and Varietal Material. After three years the MCP material had larger mean heights, mean diameters, and mean volume than the other two genetic entities. However the top five performing varietals were about 0.5 feet taller than the MCP material. Study two tested two contrasting loblolly pine ideotypes across different spacings and management intensities. After two years the crop tree ideotype and the intensive management plots had larger mean heights, mean ground-line diameters, mean volumes, and mean crown widths. Mean branch angle differed significantly between the two crown ideotypes.

This study examined the effect of manipulating forest floor and harvest residue inputs on nutrient availability and carbon content in the context of intensive forest management. Treatments were removal and addition of forest floor and slash, and a reference. Tree volume, litterfall, and root biomass were measured to assess aboveground productivity and soil moisture and temperature, available nitrogen and phosphorus, O-horizon, and mineral soil for below ground processes. Organic matter additions increased stand bole volume, carbon at 20-60cm, and total nitrogen in the mineral soil. Severely removing the floor and harvest residues did not result in any large differences in below or above ground productivity, soil carbon or nitrogen pools. Removing a potential nutrient pool did not severely affect stand productivity through mid-rotation but could be a concern in subsequent rotations.

Bareroot and containerized loblolly pine (Pinus taeda L.) were planted in December 2016 and February 2017 to compare performance across four sites in South Carolina and Georgia. Main treatments analyzed for growth differences were stocktype, genetics, and planting date. Analysis of variance results showed significant growth differences after two growing seasons for containerized over bareroot seedlings, for control-pollinated seedlings over open-pollinated and varietal seedlings, and for December-planted seedlings over February-planted seedlings. Control–pollinated seedlings demonstrated the most incremental growth between ages one and two. Site conditions dictated seedling survival to a certain degree, and bareroot seedling growth was slightly better than containerized on the least stressful site. Results uncover important trends for main effects of stocktype, genetics, and planting date, but also underscore the importance for land managers to avoid blanket reforestation prescriptions, with more emphasis placed on site-specific conditions.

There have been several studies that aim to determine the most superior Weibull parameter recovery approach of specifying a given forest stand’s Weibull diameter distribution, but no consensus has been made. The lack of agreement could be attributed to studies using different moments/percentile prediction models as well as using different plot size data. This study investigates how plot size and prediction model form affects the performance for moments, hybrid, and percentile Weibull parameter recovery approaches. Five plot sizes and three moments/percentile prediction models were used to determine their effects. Weibull parameters were calculated using each recovery method for each plot size and moments/percentile prediction model combination. Each combination’s diameter distribution was recovered and assessed using absolute error index. Results showed that plot size affected rank of precision for parameter recovery methods. Findings suggest that order statistics may be important in recovering Weibull distribution parameters from stand diameter summary statistics.

My research is focused on advancing understanding of the genomes of two important distantly related conifer species, loblolly pine (Pinus taeda L.) and bald cypress (Taxodium distichum (L.) Rich. var. distichum). Loblolly pine is the most commercially important tree crop in the United States, the major source of pulpwood for paper manufacturing, a source of quality lumber, a prime bioenergy feedstock, and an important part of the ecosystem of the southeastern U.S. Bald cypress is the dominant tree species in the aptly named ¡°cypress swamps¡± of the South.Its ecological importance to the wetlands of the southern U.S. is immeasurable. Moreover, bald cypress is a popular ornamental due to its attractive appearance and extreme resistance to pests, pathogens, and weather. Maintaining the security and productiveness of these important crop/forest species in the face of new pest, pathogen and environmental threats will require a better understanding of their genes and the structures of their genomes. We have conducted a study of loblolly pine and bald cypress in which Cot analysis and DNA sequencing of Cotiltered DNA were utilized to study genome structure. Cot analysis revealed that loblolly pine and bald cypress genomes are each composed of three major kinetic components which we have deemed highly repetitive (HR), moderately repetitive (MR), and single/low copy (SL). In loblolly pine, the HR, MR, and SL components account for 57, 24, and 10%, of genomic DNA, respectively. Of note 2.71% of random genomic sequences (i.e., 580 Mb, an amount roughly three times that of the Arabidopsis genome) show significant (bit score ≥ 60) homology to mRNA sequences. This result suggests that the loblolly pine genome contains many genes or pseudogenes, and/or gene duplications. In bald cypress, the HR, MR, and SL components account for 52, 38, and 4%, of genomic DNA, respectively. Sample sequencing was performed only on the HR component of bald cypress; sequence analysis shows only 0.81% of HR sequence reads with homology to mRNA sequences. My research provides insight into the evolution of these distant conifers and key sequence data that should greatly facilitate ongoing molecular breeding programs.

Heat shock proteins (HSPs) are expressed in response to environmental stresses. Compared to other kingdoms, plant HSP families are larger, presumably the result of adaptation to a wide range of stresses. Following on an analysis of drought stress characteristics in loblolly pine (Watkinson et al., 2003), expression patterns of HSP gene expression during photosynthetic acclimation were examined. One cycle of mild (-1Mpa) followed by two cycles of severe stress (-1.7Mpa) were probed for conditioning effects. Photosynthetic acclimation occurred after the first cycle. No acclimation occurred without the first mild cycle. Microarray/RT-PCR analyses showed that a pine homolog to GRP94 (ER-resident HSP90) was up-regulated after rehydration coincident with acclimation. This GRP94 is closely related to GRP94 from the desiccation tolerant plant X. viscosa, supporting the importance of this gene during acclimation to water deficit. HSP genes whose products localized to the mitochondrion showed gradual up-regulation after consecutive cycles of severe drought. The Arabidopsis pine GRP94 homolog, (AtHSP90-7) was then analyzed, using bioinformatics (Pati et al., 2006) and laboratory tools. Genes encoding putative candidate co-chaperones for GRP94 and other HSP90s were discovered, which contained water stress-related cis-elements. Arabidopsis (Col-0) wild type and two T-DNA insertion mutants in HSP90-7 were used to study the importance of this gene for photosynthetic acclimation. Only the mutants were able to acclimate to drought stress, with the level of AtHSP90-7 expression in the mutants being reduced compared to the wild type. AtHSP90-7 may have a different role in Arabidopsis, and its reduced expression activated other protective genes (Klein et al., 2006). Responses to extreme drought in resistant (Sullu) and susceptible (Negra Ojosa) lines of Andean potatoes were also compared in order to identify relationships between HSPs gene expression, and tolerance, defined as the ability to maintain photosynthesis at 50% after 25 days of drought and to recover from the stress. Tolerance was correlated with up-regulation of HSPs (mostly chaperonins) and antioxidant genes all of whose gene products are located in the chloroplast. / Ph. D.