Recently the most often used crane runway beams designed from rolled HEA sections as multispan beams is the topic of this doctoral thesis. It deals with the study of real stress in the cross section focused on local stress analysis in the web of cross section due to eccentricities of wheal load and on the problems of stress analysis above the middle support of multi-span beams. First, the outline of crane supporting structures, currently used methods of implementation and available stress analysis methods are summarized. Than two parametrical studies of crane runway beam design depending on statical determination of the beam, crane span, crane wheelbase and chosen quality of structural steel are presented. Following part of thesis contains the stress analysis of crane runway two-span beam in its continuous part above the middle support on implemented crane supporting structure. Experiment of crane runway two-span beams actions above the middle support are described in the last part of the thesis. Closing part of thesis summarizes results.