Made available in DSpace on 2014-10-09T12:47:57Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:07:45Z (GMT). No. of bitstreams: 1 08712.pdf: 2365459 bytes, checksum: 3be7f0aa24629302df9709de6db64f86 (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

<p>The impact of window area on the energy balance of a building was investigated by simulations in DEROB-LTH. The glazed area was varied in three types of buildings with different types of glazing and for several climates.</p><p>One low energy house was compared to a less insulated house but identical in size and layout. Three different types of glazing were used; uncoated double glazing, double glazing with one low-e coated pane and triple glazing with two low-e coated panes. Climates with variations in solar radiation, mean temperature, altitude and latitude were chosen.</p><p>The results show that if energy efficient window alternatives are chosen the flexibility of choosing the glazed area and orientation is higher. Choosing a larger area facing south resulted in a higher heating demand for uncoated double glazing in the standard house. An increased area also resulted in an increased peak load for heating for all the simulated cases. Choosing the energy efficient glazing type gave a decrease in heating demand for increased south facing glazed area in the standard house. In the low energy house the difference in heating demand between different areas was smaller than for the standard house. </p><p>An office module with two types of switchable glazing and one solar control glazing unit was used in three different climates; Stockholm, Brussels and Rome. Larger window areas increase the cooling demand but if glazing types with lower solar transmittance are used, the difference in cooling demand between different window areas decreases. An extremely large window area, however, increases the peak load both for cooling and for heating and should therefore be avoided. Energy can be saved by using switchable windows instead of solar control or in particular standard glazing.</p>

The impact of window area on the energy balance of a building was investigated by simulations in DEROB-LTH. The glazed area was varied in three types of buildings with different types of glazing and for several climates. One low energy house was compared to a less insulated house but identical in size and layout. Three different types of glazing were used; uncoated double glazing, double glazing with one low-e coated pane and triple glazing with two low-e coated panes. Climates with variations in solar radiation, mean temperature, altitude and latitude were chosen. The results show that if energy efficient window alternatives are chosen the flexibility of choosing the glazed area and orientation is higher. Choosing a larger area facing south resulted in a higher heating demand for uncoated double glazing in the standard house. An increased area also resulted in an increased peak load for heating for all the simulated cases. Choosing the energy efficient glazing type gave a decrease in heating demand for increased south facing glazed area in the standard house. In the low energy house the difference in heating demand between different areas was smaller than for the standard house. An office module with two types of switchable glazing and one solar control glazing unit was used in three different climates; Stockholm, Brussels and Rome. Larger window areas increase the cooling demand but if glazing types with lower solar transmittance are used, the difference in cooling demand between different window areas decreases. An extremely large window area, however, increases the peak load both for cooling and for heating and should therefore be avoided. Energy can be saved by using switchable windows instead of solar control or in particular standard glazing.

Made available in DSpace on 2014-10-09T12:36:04Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:22Z (GMT). No. of bitstreams: 0 / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Made available in DSpace on 2014-10-09T12:37:39Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:00:20Z (GMT). No. of bitstreams: 1 05328.pdf: 3991490 bytes, checksum: 1e2ba0f78984c357870199648df69c55 (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Made available in DSpace on 2014-10-09T12:55:25Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:06:02Z (GMT). No. of bitstreams: 0 / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Made available in DSpace on 2014-10-09T12:44:31Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:07:40Z (GMT). No. of bitstreams: 1 06917.pdf: 5485746 bytes, checksum: ddc4368b0a8bc0f9c5bc15933aa944ec (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Submitted by Claudinei Pracidelli (cpracide@ipen.br) on 2015-11-12T10:39:15Z No. of bitstreams: 0 / Made available in DSpace on 2015-11-12T10:39:15Z (GMT). No. of bitstreams: 0 / Tese (Doutorado em Tecnologia Nuclear) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Made available in DSpace on 2014-10-09T12:36:04Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:22Z (GMT). No. of bitstreams: 0 / A prolactina humana (hPRL) é um hormônio polipeptídico secretado pela hipófise anterior sob regulação do hipotálamo, envolvido em uma variedade de processos biológicos como o desenvolvimento da glândula mamária e lactação. O produto recombinante é importante no diagnóstico médico e no tratamento de insuficiência da lactação. Este hormônio pode ocorrer sob a forma de proteína não glicosilada (NG-hPRL) e glicosilada (G-hPRL), com pesos moleculares de aproximadamente 23 e 25 kilodalton (kDa), respectivamente; possui um único sítio de N-glicosilação localizado na asparagina (Asn) posição 31, que é parcialmente ocupado, representando assim um modelo particularmente interessante de glicosilação. A atividade biológica da G-hPRL é muito menor comparada à NG-hPRL (~4 vezes) e sua função fisiológica ainda não é bem definida: a porção de carboidrato parece ter um importante papel na biossíntese, secreção, atividade biológica, e sobrevivência plasmática do hormônio. O objetivo principal desse trabalho foi comparar as estruturas dos N-glicanos presentes na prolactina glicosilada hipofisária (G-hPRL-NHPP) com a recombinante. Para obter a G-hPRL recombinante foi realizada uma produção em escala laboratorial a partir de células de ovário de hamster chinês (CHO) geneticamente modificadas e adaptadas ao crescimento em suspensão. Foi adicionada, ao meio de cultura cicloheximida (CHX), cujo efeito principal foi aumentar a relação G-hPRL/NGhPRL que passou de 5% para 38%, facilitando assim a purificação da G-hPRL. A G-hPRL foi purificada em duas etapas, uma troca catiônica seguida de purificação por cromatografia liquida de alta eficiência de fase reversa (RP-HPLC) que se demonstrou eficiente na separação das duas isoformas de hPRL. A G-hPRL recombinante IPEN foi assim analisada por diversas técnicas confirmando a sua pureza e atividade biológica, incluindo comparações com outras amostras de referências de origem hipofisária adquirida junto ao National Hormone & Peptide Program (NHPP-E.U.A.) . Foi realizada também a determinação inédita de Nglicanos presentes na G-hPRL produzida por células CHO e na G-hPRL nativa, produzida pela hipófise humana, possibilitando comparar as duas estruturas de carboidratos e alcançando assim uma das principais metas desse projeto. Entre as principais diferenças encontradas nas estruturas dos dois N-glicanos, destacam-se a baixa quantidade de ácido siálico (NeuAc), a alta porcentagem de glicanos sulfatos (74,0%) e com fucose (Fuc) (93,3%) presentes na amostra hipofisária e a tendência da preparação recombinante de apresentar glicanos com maior peso molecular e com uma menor variação nas isoformas. / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Made available in DSpace on 2014-10-09T12:37:39Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:00:20Z (GMT). No. of bitstreams: 1 05328.pdf: 3991490 bytes, checksum: 1e2ba0f78984c357870199648df69c55 (MD5) / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP