The evolutionary biology of 14 species of Maniolini (Nymphalidae: Satyrinae) was studied. Electrophoretic analysis of 35 enzyme loci identified a larger number of alleles than an1 levels of polymorphism similar to those found in other Lepidoptera. In Maniola jurtina, some populations exhibited a massive heterozygote deficit and sex associated allele frequency differentiation at the GOT-l locus. Allele frequencies in pre- and post-aestivation jurtina from southern Europe were significantly different. At some loci, significant annual differences in allele frequencies were noted. A significant correlation between geographic and genetic distance in allele frequencies was observed, but no correlation was detected between heterozygosity and land area in insular populations. Cluster analysis and nonmetric multidimensional scaling per~ormed on electrophoretic data from populations of Maniola jurtina revealed a dichotomy between 'Eastern' and 'Western' subspecies groups. The analysis of Manioline species fitted existing taxonomies. Genetic differences between Maniola species were much smaller than those between Pyronia and Hyponephele species. Ultrastructural studies of the Maniola Jullien organs revealed a species-specific tooth pattern on the inner surfaces. It is suggested that these structures may be sound production mechanisms of great evolutionary significance to the species. Maniolini ova were studied and it is suggested that their form and chorionic sculpturing owe much to selection induced by oviposition strategy. Chaetotaxy of first instar larvae was undertaken and morphometric analysis of setal lengths was found to be useful, but not unambiguous. Multivariate analysis of chaetotaxy data showed a significant correlation with electrophoretic data. viii The evolution and zoogeography of Maniola is discussed. It is suggested that disjunction, founder effect, rapid post-glacial colonisation and bottlenecking have played a major roles in effecting rapid speciation. It is further suggested that all Maniola species are very recent, perhaps having evolved within the last 50,000 years, and some species almost certainly have evolved in postglacial times •

La mise en place Trame Verte et Bleue sur le territoire français a pour vocation de garantir la connectivité fonctionnelle entre habitats. C’est une des réponses politiques qui vise à enrayer le déclin actuel de la biodiversité, lie notamment à la destruction et à la dégradation des habitats. L’objectif de cette thèse est de quantifier l’effet de la connectivité, au regard d’autres facteurs environnementaux, sur la diversité des communautés et la diversité génétique des papillons de jour dans trois régions agricoles. Deux approches sont utilisées pour comprendre les processus qui sous-tendent ces patrons de diversité : i) l’étude de la communauté dans son ensemble et des groupes écologiques qui la compose, ii) l’étude de la résistance des milieux a la dispersion par génétique du paysage sur une espèce : le Myrtil (Maniola jurtina L.). Les résultats montrent que même si les caractéristiques locales jouent un rôle plus fort, la structure du paysage influence la composition des communautés. Ainsi, la quantité de terres arables réduit la richesse spécifique, la diversité génétique, et les flux de gènes chez le Myrtil. Les prairies hébergent des communautés diversifiées, incluant des espèces peu fréquentes. Les éléments linéaires enherbes supportent des communautés appauvries, mais favorisent la dispersion et la diversité génétique du Myrtil. La diversité des papillons est plus forte a proximité de boisements : les papillons pourraient bénéficier de ressources sur les lisières ; par ailleurs, les milieux boises semblent à la fois limiter les flux de gènes du Myrtil tout en accroissant sa diversité génétique. La complémentarité des milieux soulevée ici nous invite à repenser le modèle classique en ≪ sous-trames ≫ indépendantes de la Trame Verte et Bleue. Selon les enjeux de conservation, les espèces et les contextes paysagers, il est nécessaire de distinguer les situations ou, parmi un panel d’actions envisageables, améliorer la connectivité est une solution pertinente. / The implementation of the “Green and Blue Infrastructure” in France intends to ensure the functional connectivity of habitats (green veining). It is one of the nature conservation policies which aim to reduce the loss of biodiversity caused by the destruction and degradation of natural habitats. The objective of this work was to quantify the impact of connectivity, in comparison to other environmental factors, on the diversity of butterfly communities and their genetic diversity in three French agricultural regions. Two different approaches were applied to understand the underlying processes driving these diversity patterns: i) the study of the whole community and of the different ecological groups, ii) the study of landscape features resistances to butterfly dispersal using a landscape genetics approach on one species: the Meadow brown (Maniola jurtina L.). Our findings show that although local characteristics play a dominant role, landscape context does have an influence on community composition. Arable land cover decreases species diversity, and the Meadow brown genetic diversity and gene flow. Grasslands support more species rich communities, including non-frequent species. Grassy linear landscape elements host impoverished communities, but improve Meadow brown dispersal and enhance its genetic diversity. Species diversity is higher within grasslands in proximity to woody habitats: butterflies may benefit from resources on forest edges; moreover, woodlands seem to limit Meadow brown dispersal while increasing its genetic diversity. The habitat complementarity we evidenced here led us to question the classic model of ecological continuities as a collection of independent sub-networks, one for each type of habitat. According to conservation issues, species under interest and landscape contexts, we also need to identify situations where, among a broad panel of possible conservation actions, increasing connectivity is the most effective solution.