Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 4 uppsatser.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 5 uppsatser.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 5 uppsatser.

Diss. Umeå : Umeå universitet, 2007.

Diss. (sammanfattning) Uppsala : Univ., 2009. / Härtill 5 uppsatser.

Bakgrund: Att vara närvarande vid ett hjärtstopp kan vara psykiskt påfrestande för vilken sjuksköterska som helst. Genom att kontinuerligt få träning och uppdatera sin kunskap gällande HLR ger det sjuksköterskan möjlighet att vid senare tillfälle reflektera över händelsen och därmed kunna släppa situationen. Syfte: Var att belysa allmänsjuksköterskans chans till reflektion efter att aktivt deltagit vid ett hjärtstopp. Metod: Var en kvalitativ intervjustudie med semistrukturerade intervjufrågor. Fem stycken sjuksköterskor intervjuades under november 2017. Intervjuerna transkriberades och efter en manifest analysmetod delades innehållet upp i fyra kategorier med två underliggande kategorier tillhörande varje kategori. Resultat: Det visade att chansen till reflektion var bristande. Vissa informanter upplevde att de fick chans till reflektion, medan andra ansåg att det var en brist. Resultatet visade även skillnaden på hur snabbt reflektionen skedde efter ett hjärtstopp mellan avdelningar. Informanterna uttryckte även detta som en brist, då de ibland inte fick utlopp för sina egna känslor och tankar. Slutsats: Efter avslutad studie syns bristen vara för stor då alla borde få samma chans till reflektion efter avslutad HLR.

digitalisering@umu.se

Genetic polymorphism is by definition the occurrence in the same population of two or more alleles at one locus,each with a frequency high enough not to be maintained by recurrent mutation only (1). Among the human plasmaproteins two major categories of polymorphism have been described, allotypic and electrophoretic heterogeneity. Allotypy is defined by Oudin as individual antigenic differences among proteins within a species (2). The first discovered polymorphism of this category was the Gm system of immunoglobulin G by Grubb (3). The first described electrophoretic heterogeneity in plasma proteins was theHp (haptoglobin) system discovered by Smithies (4). Sincethen genetic variants of several other human plasma proteins have been found. This dissertation is concerned with thegenetic and immunological aspects of the polymorphism of the third component of human complement, C3. / digitalisering@umu.se

Obesity leading to Type-2-diabetes is a major health issue all over the world. Obesity characterized by expansion of adipose tissue, in particular white adipose tissue (WAT) which controls the metabolic physiology in the body by secreting proteins like adiponectin and resistin. Adiponectin has a protective role against diabetes development whereas resistin causes insulin resistance. Protein folding, maturation and translocation is performed by the Endoplasmic reticulum using calcium ions. The calcium homeostasis is maintained by calcium pumps and channels, chief of this is sarco-/endoplasmic reticulum (SR/ER) Ca2+ ATPase pump (SERCA) which restores calcium back to the ER. To study the effect of SERCA2 on adiponectin and resistin secretion in different adipose tissue depots using an adipocyte specific tamoxifen-inducible SERCA2 Knock-out mice, short term secretion experiments were performed. Chemical inhibition of SERCA2 and ER stress was performed in in-vitro experiments using adipocyte like 3T3-L1 cell line. The experiments revealed that SERCA2 dysfunction led to decrease in adiponectin and resistin secretion in normal and stimulant conditions in both male and female mice. In-vitro experiments revealed that ER stress led to misfolded protein accumulation affecting exocytotic events of adiponectin containing vesicles. Therapeutic agents can be formulated to tackle the SERCA2 dysfunction and to maintain calcium homeostasis by identifying these key mechanisms for diabetes and related metabolic disorders.

Tuberculosis (TB) disease is a world-wide spread disease caused by Mycobacterium tuberculosis which affects a significant percentage of the world population. The disease is often localized in the lungs, but can further develop and spread to other organs and is then called extrapulmonary TB (EPTB). The diagnosis of TB by culture is time consuming and requires specialised facilities. The use of DNA amplification is therefore an advantageous and rapid diagnostic tool. One such tool is the GeneXpert system which also can detect resistance to rifampicin (Rif) in samples. Although the system is designed to mainly analyse unprocessed sputum samples, there is a possibility to also analyse formalin-fixed paraffin-embedded (FFPE) tissue. The project's aim was to compare different pretreatment methods for processing FFPE tissue samples along with the application of Xpert MTB/Rif Ultra assay to improve EPTB case diagnosis. Tissue sections from eleven different TB negative FFPE tissue samples were used to assess three different pretreatment methods. Tissue sections from seven TB positive FFPE tissue samples were later used to validate the application of Xpert MTB/Rif Ultra assay usage. The current lab developed pretreatment method were after statistical testing and handling evaluation chosen and applied for the TB positive FFPE tissue samples. Results from the seven historical patient samples gave a 100 % positive Mtb detection result. The project's results show that the pretreatment of FFPE tissue can be applied before the Xpert MTB/Rif Ultra assay cartridges for processing on the GeneXpert instrument.