31 |
Investigation into the expression, role and regulation of the Myc oncogene during vertebrate embryonic body axis elongationMastromina, Ioanna January 2017 (has links)
No description available.
|
32 |
Function of two closely related fibroblast growth factors in early mesoderm development of Drosophila melanogasterKlingseisen, Anna January 2009 (has links)
Thisbe (Ths) and Pyramus (Pyr) are the ligands for the Fibroblast-Growth-Factor (FGF)receptor Heartless (Htl), which is expressed in all mesodermal cells during gastrulation. To understand how these two FGFs orchestrate mesoderm spreading in gastrulation and mesoderm differentiation during organogenesis, loss and gain of function studies were performed. In an approach of functional analysis, a single mutant allele of ths was generated, ths759, for comparison of the single mutant conditions of ths and the null mesodermal cells to migrate and differentiate in a precise pattern.
|
33 |
Genetic analysis of Drosophila NSF function /Golby, Jessica A. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 120-140).
|
34 |
Signal transport, levels and integration : new lessons from wingless regulation of drosophila mesoderm development /Cox, Virginia Taylor. January 2004 (has links)
Thesis (Ph. D.)--Cornell University, January, 2004. / Vita. Includes bibliographical references (leaves [170]-191).
|
35 |
Zebrafish as a vertebrate model to study retinoic acid signalling in head mesoderm and pectoral fin development and to investigate non-ion channel epilepsiesGibert, Yann. January 2004 (has links)
Konstanz, Univ., Diss., 2004.
|
36 |
Requirement of hand2 in noradrenergic differentiation of sympathetic neurons and zebrafish hatchback required for neural crest and lateral mesoderm developmentLucas, Marsha Elaine, January 2008 (has links)
Thesis (Ph. D.)--Ohio State University, 2008.
|
37 |
Dynamic Delta‐like1 expression in presomitic mesoderm cells during somite segmentation / 体節形成における未分節中胚葉細胞のDelta-like 1の発現動態Takagi, Akari 23 March 2020 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第22350号 / 医博第4591号 / 新制||医||1042(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 斎藤 通紀, 教授 浅野 雅秀, 教授 安達 泰治 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
|
38 |
The Role of Cdx in Mesoderm Cell Fate SpecificationFoley, Tanya 24 January 2022 (has links)
Roles for the Cdx transcription factors during anteroposterior patterning and development of the posterior embryo have been well described, yet little is known about Cdx functions during mesoderm specification. In the studies presented here, novel roles for Cdx during gastrulation are presented. In the first of two studies, the role of Cdx factors during cardiac mesoderm specification is investigated. We demonstrate that Cdx factors epigenetically restrict cardiac progenitor specification, preventing the ectopic expression of cardiogenic genes within progenitor mesodermal populations. We provide evidence to suggest that this occurs through interaction with Brg1, a subunit of the SWI/SNF chromatin remodeling complex, and propose a molecular mechanism by which Cdx-mediated recruitment of the SWI/SNF complex is required to maintain repression of cardiac targets at developmental stages where Cdx transcription factors are no longer expressed.
Following this, Cdx-dependent gene expression programs were identified in the extra-embryonic yolk sac. RNA-sequencing of Cdx-mutant yolk sacs revealed novel Cdx-dependent gene targets involved in ion and nutrient transport, functions analogous to those previously described for Cdx in the adult intestinal epithelium. Subsequent experimentation revealed that, for a subset of these targets, regulation correlates with the maintenance of H3K27me3 marks. Finally, we provide evidence to suggest Cdx-dependent H3K27me3 marks are established at early developmental stages, when yolk sac progenitors are specified from the streak.
Together, these studies describe novel roles for Cdx factors in mesoderm specification during gastrulation, and evoke molecular mechanisms by which Cdx might program early mesodermal populations for gene expression at later developmental stages through interactions with epigenetic regulatory complexes.
|
39 |
Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. / ラミニンによって方向づけられるヒト多能性幹細胞からの効率的な血管内皮細胞分化誘導Ohta, Ryo 23 May 2017 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第20562号 / 医博第4247号 / 新制||医||1022(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 山下 潤, 教授 江藤 浩之, 教授 開 祐司 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
|
40 |
TRANSDUCING MURINE EMBRYONIC STEM CELLS TO MESODERM LINEAGEWest, Alexis Ronna 01 May 2023 (has links) (PDF)
With so many deaths around the world being due to cardiovascular disease, there is great demand for an unlimited supply of cells for application in regenerative medicine. However, the control of directing pluripotent cells into mesoderm lineage which gives rise to cardiac muscle cells remains poor. Here in this work, the synergistic effect of chemical and mechanical signaling in driving cells towards mesoderm germ-layer was investigated. Transgenic reporter mouse embryonic stem cells were used in this study. The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. In the presence of fibronectin and a small molecule called leukemia inhibitory factor (LIF), which is known to maintain pluripotency and self-renewal, mouse embryonic stem cells responded by exhibiting expressions of both GFP and DsRed. To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. The differentiation was found to have a pronounced effect in the presence of fibronectin and DLL1 protein together with the withdrawal of LIF. This is a proof-of-concept that mechanical signaling together with synergistic chemical signaling can drive pluripotent cells towards mesoderm lineage. Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. Collectively, this study shows the importance of chemical, but also the importance of mechanical signaling to transduce cells to mesoderm.
|
Page generated in 0.0549 seconds