The metal mining history goes thousands of years back. The downside of the mining industry is the large amount of tailings created during the mining operations. Drainage and leaching of heavy metals from these tailings may cause major environmental problems including acid mine drainage and leaching of heavy metals. This study examines a pond located near an old mining site, where part of the old mining facility has been recently (1990) restored. The objective was to assess eventual metal contamination from historical mining and the recently performed restoration using a sediment core as a natural archive of historical metal inputs to the pond. I found that the concentration of lead, copper, zinc and nickel were elevated in comparison to background levels in sediment. Elevated concentrations were found in the deep layer of the sediment core, 14-20 cm with an estimated age of ca 100 years. The highest concentrations were found near the surface (3-4 cm depth). Conclusion of the study was that the early mining operations has affected the ponds metal contamination history. The restoration of the mining site had weak impact on the pond, but may contribute to the high metal concentration in recent sediments.

The overall objective of this thesis was to determine selenium (Se) levels in the major compartments of aquatic ecosystems and correlate these data with potential Se effects on early life stages of two native fish species. This work was conducted at two uranium (U) mines located in northern Saskatchewan, Key Lake mine and McClean Lake mine. In addition, a site fidelity study was conducted at Key Lake mine to evaluate movement patterns of northern pike inhabiting lakes receiving effluent discharges.<p> At Key Lake mine, Se was accumulated and biomagnified several orders of magnitude higher than its concentration in water (0.7-2.7 µg/L) in lakes receiving discharges, with Se in prey organisms reaching levels above the proposed 3-11 µg/g dry weight (DW) dietary toxicity threshold for fish. Increased concentrations of Se in aquatic biota led to an increase in the Se concentrations in eggs and tissues of northern pike that rely on these organisms as food sources. Furthermore, increases in the incidence of Se-induced deformities above 30% were recorded in fry originating from adults exposed to high levels of dietary Se (> 3µg/g, DW). The increased frequency of deformities found in northern pike fry was associated with a significant increase in the level of Se in northern pike eggs from exposure sites (31.28 - 48.23 µg/g DW) compared to reference (3.19 ± 0.29 µg/g DW).<p> At McClean Lake mine, Se was accumulated and biomagnified through the aquatic food chain with concentrations in some biota groups (e.g., forage fish) exceeding the lower limit (> 3µg/g DW) of the 3-11µg/g (DW) threshold for dietary Se toxicity in fish. Although both northern pike and white sucker females collected from the exposure site showed greater levels of Se in egg and tissues compared to fish collected from a reference site (likely caused by exposure to elevated levels of Se in prey organisms), no increases in Se-induced deformities were found in the developing fish larvae. The lack of a toxic response in fish larvae is in agreement with Se thresholds for early life stage deformities, with egg Se concentrations in northern pike and white sucker collected at the exposure site below the proposed 10 µg/g (DW) threshold associated with the presence of developmental abnormalities. The applicability of the proposed 7.91 µg/g (whole body, DW) Se toxicity threshold to cold water fish is controversial given that most of the research has focused on warm water fish. Therefore, there is an urgent need to conduct studies that allow us to better understand the environmental fate and effects of Se in north temperate (cold water) aquatic systems. The results of my research will contribute valuable information for the establishment of a realistic and environmentally relevant Se threshold for the protection of fish populations in Canadian waters. During the site fidelity study, fish locations were seasonally and daily recorded using a Lotek SRX_400 receiver with handheld Yagi antenna. The results suggest that tagged pike did not migrate out of the study area throughout the study period, with the mean distance traveled ranging from 50 to 400 m. Differences in movement (distance traveled) and home range were found between reference and exposure sites. Overall data suggest that radio-telemetry is a useful tool in environmental studies. This information on northern pike behavior will be valuable towards developing non-lethal sampling methods that could be applied for assessing the effects of industrial discharges in north temperate aquatic ecosystems.

Previous studies at the Key Lake uranium mill (Saskatchewan, Canada) suggested the complex effluent discharged alters energetic stores of resident fish species. A second study at the same site demonstrated certain fish from lakes downstream of the mill produce larvae with elevated incidence of developmental deformities. The mechanisms by which energy homeostasis is affected in fish downstream of the Key Lake uranium mill are unknown, and the effects of deformities and altered metabolism on swimming ability have not been explored. Therefore, the overall objective of this thesis was to investigate whether effluent exposed fish exhibited differences in swimming performance and energy homeostasis. To achieve this objective two experiments were conducted. In the first experiment juvenile spottail shiner (Notropis hudsonius) were collected from a lake downstream of the Key Lake uranium mill, and compared to fish collected from a nearby reference lake. In the second experiment larvae were collected from laboratory raised fathead minnow (Pimephales promelas) exposed to 5% diluted uranium mill effluent or control (dechlorinated municipal) water, and reared in the same treatments to 60 days post hatch (dph). No gross deformities were observed in any fish, and only shiner collected from the exposure lake in the field experiment had enlarged heart ventricles relative to body size compared to fish from the reference lake. Swimming performance was similar between shiner from the exposure and reference lakes in the field study, but effluent exposure impaired swimming ability in 60 dph fathead minnow in the laboratory experiment compared to fish from the control water treatment. After swimming performance tests fish were considered fatigued and metabolic endpoints were compared to non-fatigued fish. In both non-fatigued and fatigued shiner, liver glycogen was significantly greater in fish collected from the exposure lake compared to the reference lake. There was no difference in liver triglycerides in non-fatigued shiner between lakes, but liver triglycerides decreased after swimming in the field study reference fish. Muscle energy stores were unaffected by site or swimming in the field experiment. Conversely, whole body triglycerides and glycogen were similar between treatments in non-fatigued fathead minnow in the laboratory experiment. Swimming significantly decreased whole body triglycerides in fathead minnow from both treatments, but whole body glycogen was unaffected. In the field experiment blood endpoints (hematocrit, plasma glucose, lactate) in fatigued and non-fatigued shiner from both lakes further supported the possibility of altered intermediary metabolism or blunted stress response in fish downstream of the Key Lake uranium mill. In the field study, shiner muscle citrate synthase activity (an indicator of tissue aerobic capacity) was similar between lakes, but muscle âhydroxyacyl coenzyme A dehydrogenase activity (an indicator of tissue lipolytic capacity) was elevated. In contrast, laboratory fathead minnow whole body âhydroxyacyl coenzyme A dehydrogenase activity was similar between treatments, but citrate synthase activity was significantly lower in fathead minnow from the 5% effluent treatment. In summary, shiner from the exposure lake in the field experiment had similar swimming endurance and greater energy stores compared to fish from the reference lake, despite metabolic alterations. Fathead minnow from the 5% effluent treatment in the laboratory experiment had reduced swimming endurance that was matched by reduced whole body citrate synthase activity, but no other metabolic alterations were observed. Therefore, effluent exposure caused metabolic alterations in both fathead minnow and shiner, but specific effects between experiments were inconsistent. Overall, the physiological significance of the metabolic and swimming effects of effluent exposure is unclear, but suggests discharged effluent has the potential to negatively affect wild fish survivability.

The overall objective of the research conducted and described in this thesis was to develop an environmentally relevant bioassay to assess the effects of complex effluents on a sentinel fish species. A short-term fathead minnow (FHM) reproductive bioassay was utilized to assess the effects of industrial effluents on multiple levels of biological organization (sub-organismal to population endpoints). The FHM bioassay was tested in both lab and on-site investigations using an artificial stream system. The incorporation of trophic-transfer into the bioassay was also developed to quantify the importance of contaminated food as a source of exposure. This work was conducted in two key phases. Phase I focused on testing and developing the FHM bioassay, in the lab and on-site with pulp mill effluent (PME), to firstly document response patterns and, secondly, to conduct an investigation of cause study. Phase II focused on developing the trophic-transfer system to document responses to metal mine effluent (MME) in the lab and on-site in an artificial stream system. Development of the trophic-transfer system was also conducted during this phase to compare responses to standard water-only exposures. <p>In Phase I, exposure to PME in both the lab and field studies resulted in disruptions in egg production and spawning events. By focusing on identifying response patterns I was able to determine that the effects observed were indicative of an estrogenic response. I was also able to identify a process stream that was the potential cause of responses observed after exposure to final effluent. Isolation of this process stream will assist the mill in developing approaches for future mitigation. The results from this research will also provide additional data for the environmental effects monitoring (EEM) program for pulp and paper and investigation of cause studies on a national basis. <p> In Phase II, in both the field and laboratory investigations, significant decreases in reproductive output (egg production and spawning events) were observed in the water-only system exposures. Significant decreases in hatching success and increases in deformities were observed in the trophic-transfer system only, suggesting that the combination of both food and water was important in assessing the effects on the F1 generation. Overall, the responses in the trophic-transfer system were not comparable between the lab and field studies. In the lab study, significant decreases in reproductive output occurred, compared to the field study where significant increases in egg production and spawning events occurred. In addition, the effects on the F1 generation in the field study were not as severe as those observed in the lab investigation. It was concluded that the presence of reference water and the environment within the trophic-transfer system were responsible for this reduction in toxicity. <p> Phases I and II of this research have made significant contributions to artificial stream development within Canada for the assessment of industrial effluents and their effects on aquatic biota. The results from these studies have also demonstrated that environmentally relevant testing is essential if we are to accurately assess effects on aquatic biota. Future development and application of this bioassay should be towards developing a standardized approach for not only assessing the effects of industrial effluents in a comparative manner, but also in investigation of cause studies.

The overall objective of the research conducted and described in this thesis was to develop an environmentally relevant bioassay to assess the effects of complex effluents on a sentinel fish species. A short-term fathead minnow (FHM) reproductive bioassay was utilized to assess the effects of industrial effluents on multiple levels of biological organization (sub-organismal to population endpoints). The FHM bioassay was tested in both lab and on-site investigations using an artificial stream system. The incorporation of trophic-transfer into the bioassay was also developed to quantify the importance of contaminated food as a source of exposure. This work was conducted in two key phases. Phase I focused on testing and developing the FHM bioassay, in the lab and on-site with pulp mill effluent (PME), to firstly document response patterns and, secondly, to conduct an investigation of cause study. Phase II focused on developing the trophic-transfer system to document responses to metal mine effluent (MME) in the lab and on-site in an artificial stream system. Development of the trophic-transfer system was also conducted during this phase to compare responses to standard water-only exposures. <p>In Phase I, exposure to PME in both the lab and field studies resulted in disruptions in egg production and spawning events. By focusing on identifying response patterns I was able to determine that the effects observed were indicative of an estrogenic response. I was also able to identify a process stream that was the potential cause of responses observed after exposure to final effluent. Isolation of this process stream will assist the mill in developing approaches for future mitigation. The results from this research will also provide additional data for the environmental effects monitoring (EEM) program for pulp and paper and investigation of cause studies on a national basis. <p> In Phase II, in both the field and laboratory investigations, significant decreases in reproductive output (egg production and spawning events) were observed in the water-only system exposures. Significant decreases in hatching success and increases in deformities were observed in the trophic-transfer system only, suggesting that the combination of both food and water was important in assessing the effects on the F1 generation. Overall, the responses in the trophic-transfer system were not comparable between the lab and field studies. In the lab study, significant decreases in reproductive output occurred, compared to the field study where significant increases in egg production and spawning events occurred. In addition, the effects on the F1 generation in the field study were not as severe as those observed in the lab investigation. It was concluded that the presence of reference water and the environment within the trophic-transfer system were responsible for this reduction in toxicity. <p> Phases I and II of this research have made significant contributions to artificial stream development within Canada for the assessment of industrial effluents and their effects on aquatic biota. The results from these studies have also demonstrated that environmentally relevant testing is essential if we are to accurately assess effects on aquatic biota. Future development and application of this bioassay should be towards developing a standardized approach for not only assessing the effects of industrial effluents in a comparative manner, but also in investigation of cause studies.

The overall objective of this thesis was to determine selenium (Se) levels in the major compartments of aquatic ecosystems and correlate these data with potential Se effects on early life stages of two native fish species. This work was conducted at two uranium (U) mines located in northern Saskatchewan, Key Lake mine and McClean Lake mine. In addition, a site fidelity study was conducted at Key Lake mine to evaluate movement patterns of northern pike inhabiting lakes receiving effluent discharges.<p> At Key Lake mine, Se was accumulated and biomagnified several orders of magnitude higher than its concentration in water (0.7-2.7 µg/L) in lakes receiving discharges, with Se in prey organisms reaching levels above the proposed 3-11 µg/g dry weight (DW) dietary toxicity threshold for fish. Increased concentrations of Se in aquatic biota led to an increase in the Se concentrations in eggs and tissues of northern pike that rely on these organisms as food sources. Furthermore, increases in the incidence of Se-induced deformities above 30% were recorded in fry originating from adults exposed to high levels of dietary Se (> 3µg/g, DW). The increased frequency of deformities found in northern pike fry was associated with a significant increase in the level of Se in northern pike eggs from exposure sites (31.28 - 48.23 µg/g DW) compared to reference (3.19 ± 0.29 µg/g DW).<p> At McClean Lake mine, Se was accumulated and biomagnified through the aquatic food chain with concentrations in some biota groups (e.g., forage fish) exceeding the lower limit (> 3µg/g DW) of the 3-11µg/g (DW) threshold for dietary Se toxicity in fish. Although both northern pike and white sucker females collected from the exposure site showed greater levels of Se in egg and tissues compared to fish collected from a reference site (likely caused by exposure to elevated levels of Se in prey organisms), no increases in Se-induced deformities were found in the developing fish larvae. The lack of a toxic response in fish larvae is in agreement with Se thresholds for early life stage deformities, with egg Se concentrations in northern pike and white sucker collected at the exposure site below the proposed 10 µg/g (DW) threshold associated with the presence of developmental abnormalities. The applicability of the proposed 7.91 µg/g (whole body, DW) Se toxicity threshold to cold water fish is controversial given that most of the research has focused on warm water fish. Therefore, there is an urgent need to conduct studies that allow us to better understand the environmental fate and effects of Se in north temperate (cold water) aquatic systems. The results of my research will contribute valuable information for the establishment of a realistic and environmentally relevant Se threshold for the protection of fish populations in Canadian waters. During the site fidelity study, fish locations were seasonally and daily recorded using a Lotek SRX_400 receiver with handheld Yagi antenna. The results suggest that tagged pike did not migrate out of the study area throughout the study period, with the mean distance traveled ranging from 50 to 400 m. Differences in movement (distance traveled) and home range were found between reference and exposure sites. Overall data suggest that radio-telemetry is a useful tool in environmental studies. This information on northern pike behavior will be valuable towards developing non-lethal sampling methods that could be applied for assessing the effects of industrial discharges in north temperate aquatic ecosystems.

Previous studies at the Key Lake uranium mill (Saskatchewan, Canada) suggested the complex effluent discharged alters energetic stores of resident fish species. A second study at the same site demonstrated certain fish from lakes downstream of the mill produce larvae with elevated incidence of developmental deformities. The mechanisms by which energy homeostasis is affected in fish downstream of the Key Lake uranium mill are unknown, and the effects of deformities and altered metabolism on swimming ability have not been explored. Therefore, the overall objective of this thesis was to investigate whether effluent exposed fish exhibited differences in swimming performance and energy homeostasis. To achieve this objective two experiments were conducted. In the first experiment juvenile spottail shiner (Notropis hudsonius) were collected from a lake downstream of the Key Lake uranium mill, and compared to fish collected from a nearby reference lake. In the second experiment larvae were collected from laboratory raised fathead minnow (Pimephales promelas) exposed to 5% diluted uranium mill effluent or control (dechlorinated municipal) water, and reared in the same treatments to 60 days post hatch (dph). No gross deformities were observed in any fish, and only shiner collected from the exposure lake in the field experiment had enlarged heart ventricles relative to body size compared to fish from the reference lake. Swimming performance was similar between shiner from the exposure and reference lakes in the field study, but effluent exposure impaired swimming ability in 60 dph fathead minnow in the laboratory experiment compared to fish from the control water treatment. After swimming performance tests fish were considered fatigued and metabolic endpoints were compared to non-fatigued fish. In both non-fatigued and fatigued shiner, liver glycogen was significantly greater in fish collected from the exposure lake compared to the reference lake. There was no difference in liver triglycerides in non-fatigued shiner between lakes, but liver triglycerides decreased after swimming in the field study reference fish. Muscle energy stores were unaffected by site or swimming in the field experiment. Conversely, whole body triglycerides and glycogen were similar between treatments in non-fatigued fathead minnow in the laboratory experiment. Swimming significantly decreased whole body triglycerides in fathead minnow from both treatments, but whole body glycogen was unaffected. In the field experiment blood endpoints (hematocrit, plasma glucose, lactate) in fatigued and non-fatigued shiner from both lakes further supported the possibility of altered intermediary metabolism or blunted stress response in fish downstream of the Key Lake uranium mill. In the field study, shiner muscle citrate synthase activity (an indicator of tissue aerobic capacity) was similar between lakes, but muscle âhydroxyacyl coenzyme A dehydrogenase activity (an indicator of tissue lipolytic capacity) was elevated. In contrast, laboratory fathead minnow whole body âhydroxyacyl coenzyme A dehydrogenase activity was similar between treatments, but citrate synthase activity was significantly lower in fathead minnow from the 5% effluent treatment. In summary, shiner from the exposure lake in the field experiment had similar swimming endurance and greater energy stores compared to fish from the reference lake, despite metabolic alterations. Fathead minnow from the 5% effluent treatment in the laboratory experiment had reduced swimming endurance that was matched by reduced whole body citrate synthase activity, but no other metabolic alterations were observed. Therefore, effluent exposure caused metabolic alterations in both fathead minnow and shiner, but specific effects between experiments were inconsistent. Overall, the physiological significance of the metabolic and swimming effects of effluent exposure is unclear, but suggests discharged effluent has the potential to negatively affect wild fish survivability.

In Vietnam, soil contamination with lead and cadmium at very high level was investigated anddiscovered in the surrounding areas of zinc-lead mining and processing factory in Tan Long (Dong Hy district, Thai Nguyen province) and around the lead-recycling smelter in Chi Dao (Van Lam district, Hung Yen province). The survey on soil contaminated by arsenic due to the tin mining and sifting activities in Ha Thuong (Dai Tu district, Thai Nguyen province) was also carried out. In Tan Long, the concentrations of lead and cadmium in the old solid waste dump from zinc-lead factory varied from 1,100 to 13,000 mg·kg-1, and from 11.34 to 61.04 mg·kg-1, respectively. Soil Pollution Indexes (SPI) of lead and cadmium were highest in the old solid waste dump area, followed by the ones in the rice paddy soils. In Chi Dao, the soils of many sites were polluted with lead and cadmium such as in the gardens of lead-recycling households where the concentrations of lead and cadmium were 7,000 - 15,000 mg·kg-1 and 1.8 - 3.6 mg·kg-1. In rice paddies, the soils were also polluted by lead. SPI of lead in paddy soil areas within 300 m radius from the lead smelter were from 3.6 to 100 fold higher than the safe limit. The sediment from the ditch near the lead smelters contained extremely high levels of lead (7,000 - 110,000 mg·kg-1) and cadmium (3.8 - 17.7 mg·kg-1). The tin mining and sifting activities in Ha Thuong was the cause for the arsenic contamination of the soil in this area. The arsenic contents in soils at all locations investigated were higher than 320 mg·kg-1 (dry weight) and up to 3,809 mg·kg-1. / Tại Việt Nam, đất bị ô nhiễm bởi chì và ca-đi-mi với hàm lượng cao đã được điều tra phát hiện ở các khu vực phụ cận của nhà máy khai thác và chế biến kẽm/chì thuộc địa phận xã Tân Long, huyện Đồng Hỷ, tỉnh Thái Nguyên và các khu vực phụ cận của lò tái chế chì thuộc địa phận xã Chỉ Đạo, huyện Văn Lâm, tỉnh Hưng Yên. Sự khảo sát đất bị ô nhiễm bởi a-sen do các hoạt động khai thác và tuyển thiếc ở xã Hà Thượng, huyện Đại Từ, tỉnh Thái Nguyên cũng đã được tiến hành. Tại xã Tân Long, nồng độ chì và ca-đi-mi trong bãi chất thải rắn cũ từ nhà máy sản xuất kẽm chì là 1.100 - 1.300 mg.kg-1 và từ 11,34 đến 61,04 mg.kg-1, tương ứng. Chỉ số ô nhiễm đất (SPI) của chì và ca-đi-mi cao nhất trong khu vực đổ chất thải rắn cũ, tiếp theo sau là ở các ruộng lúa. Tại xã Chỉ Đạo, đất ở nhiều địa điểm đã bị ô nhiễm chì và ca-đi-mi chẳng hạn như trong khu vườn của các hộ gia đình tái chế chì, nồng độ chì và ca-đi-mi là 7.000 - 15.000 mg.kg-1 và 1,8 - 3,6 mg.kg-1. Trong cánh đồng lúa, đất cũng bị ô nhiễm bởi chì. Chỉ số ô nhiễm đất của chì (SPI-Pb) trong cánh đồng lúa trong vòng bán kính 300 m từ lò tái chế chì cao hơn giới hạn của đất an toàn từ 3,6 đến 100 lần. Trầm tích thu từ kênh gần lò tái chế chì chứa hàm lượng chì rất cao (.7000 - 110.000 mg.kg-1) và ca-đi-mi (3,8 - 17,7 mg.kg-1). Việc khai thác và tuyển thiếc tại xã Hà Thượng đã gây ra ô nhiễm a-sen trong đất tại khu vực này. Hàm lượng a-sen trong đất tại tất cả các địa điểm nghiên cứu cao hơn 320 mg.kg-1 (trọng lượng khô), đặc biệt là lên đến 3809 mg.kg-1.

This is a study of cobblestone tools from metalliferous mine sites in England and Wales dated to the Bronze Age which were most probably used to extract copper ore. The site assemblages studied are from the Great Orme, Copa Hill in Cwmystwyth, Nantyreira, Parys Mountain and Alderley Edge. The majority of the tools are hammerstones used to mine and beneficiate metal ore. Some of these have been modified to facilitate hafting. The functional uses of these tools have been identified by the form and position of use- wear on a macroscopic level. The recording procedure encompasses cobble morphology, the degree, type and direction of use, breakage patterns, the reuse of tools and tool fragments and the classification of hafting modification. The possibility of tool specialization within tool types has been examined by the analysis of use-wear and cobble shape and size. The analysis of stone hammer size suggests that the Great Orme material is related to specific working techniques employed to extract ore from the different types of ore deposits. Ore comminution has been demonstrated to have been generally achieved by ‘block-on-block’ crushing with flat-sided hammers. Conclusions are draw on the overall efficiency of ore extraction in the Bronze Age and theories on the organization of mining are presented. The sedimentary form of the cobblestone tools has also been examined, including the identification of natural abrasion marks and features. At Cwmystwyth and the Great Orme possible sources of cobblestones have been studied in order to assess the nature of cobble selection. / SERC studentship / The additional content files which accompanied the thesis are not available on Bradford Scholars, but are available from the British Library Ethos Service.

In Vietnam, soil contamination with lead and cadmium at very high level was investigated anddiscovered in the surrounding areas of zinc-lead mining and processing factory in Tan Long (Dong Hy district, Thai Nguyen province) and around the lead-recycling smelter in Chi Dao (Van Lam district, Hung Yen province). The survey on soil contaminated by arsenic due to the tin mining and sifting activities in Ha Thuong (Dai Tu district, Thai Nguyen province) was also carried out. In Tan Long, the concentrations of lead and cadmium in the old solid waste dump from zinc-lead factory varied from 1,100 to 13,000 mg·kg-1, and from 11.34 to 61.04 mg·kg-1, respectively. Soil Pollution Indexes (SPI) of lead and cadmium were highest in the old solid waste dump area, followed by the ones in the rice paddy soils. In Chi Dao, the soils of many sites were polluted with lead and cadmium such as in the gardens of lead-recycling households where the concentrations of lead and cadmium were 7,000 - 15,000 mg·kg-1 and 1.8 - 3.6 mg·kg-1. In rice paddies, the soils were also polluted by lead. SPI of lead in paddy soil areas within 300 m radius from the lead smelter were from 3.6 to 100 fold higher than the safe limit. The sediment from the ditch near the lead smelters contained extremely high levels of lead (7,000 - 110,000 mg·kg-1) and cadmium (3.8 - 17.7 mg·kg-1). The tin mining and sifting activities in Ha Thuong was the cause for the arsenic contamination of the soil in this area. The arsenic contents in soils at all locations investigated were higher than 320 mg·kg-1 (dry weight) and up to 3,809 mg·kg-1. / Tại Việt Nam, đất bị ô nhiễm bởi chì và ca-đi-mi với hàm lượng cao đã được điều tra phát hiện ở các khu vực phụ cận của nhà máy khai thác và chế biến kẽm/chì thuộc địa phận xã Tân Long, huyện Đồng Hỷ, tỉnh Thái Nguyên và các khu vực phụ cận của lò tái chế chì thuộc địa phận xã Chỉ Đạo, huyện Văn Lâm, tỉnh Hưng Yên. Sự khảo sát đất bị ô nhiễm bởi a-sen do các hoạt động khai thác và tuyển thiếc ở xã Hà Thượng, huyện Đại Từ, tỉnh Thái Nguyên cũng đã được tiến hành. Tại xã Tân Long, nồng độ chì và ca-đi-mi trong bãi chất thải rắn cũ từ nhà máy sản xuất kẽm chì là 1.100 - 1.300 mg.kg-1 và từ 11,34 đến 61,04 mg.kg-1, tương ứng. Chỉ số ô nhiễm đất (SPI) của chì và ca-đi-mi cao nhất trong khu vực đổ chất thải rắn cũ, tiếp theo sau là ở các ruộng lúa. Tại xã Chỉ Đạo, đất ở nhiều địa điểm đã bị ô nhiễm chì và ca-đi-mi chẳng hạn như trong khu vườn của các hộ gia đình tái chế chì, nồng độ chì và ca-đi-mi là 7.000 - 15.000 mg.kg-1 và 1,8 - 3,6 mg.kg-1. Trong cánh đồng lúa, đất cũng bị ô nhiễm bởi chì. Chỉ số ô nhiễm đất của chì (SPI-Pb) trong cánh đồng lúa trong vòng bán kính 300 m từ lò tái chế chì cao hơn giới hạn của đất an toàn từ 3,6 đến 100 lần. Trầm tích thu từ kênh gần lò tái chế chì chứa hàm lượng chì rất cao (.7000 - 110.000 mg.kg-1) và ca-đi-mi (3,8 - 17,7 mg.kg-1). Việc khai thác và tuyển thiếc tại xã Hà Thượng đã gây ra ô nhiễm a-sen trong đất tại khu vực này. Hàm lượng a-sen trong đất tại tất cả các địa điểm nghiên cứu cao hơn 320 mg.kg-1 (trọng lượng khô), đặc biệt là lên đến 3809 mg.kg-1.