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11	The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro Bioreactors Schäpe, Stephanie Serena, Krause, Jannike Lea, Engelmann, Beatrice, Fritz-Wallace, Katarina, Schattenberg, Florian, Liu, Zishu, Müller, Susann, Jehmlich, Nico, Rolle-Kampczyk, Ulrike, Herberth, Gunda, von Bergen, Martin 11 April 2023 (has links) Many functions in host–microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota. To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function. After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances. For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations. Specifically, B. thetaiotaomicron was identified to be affected in terms of butyrate metabolism. However, communities could recover to the original state afterward. This study shows that SIHUMIx showed high structural stability when TT changed—even four-fold. Resistance values remained high, which suggests that TTs did not interfere with the structure of the community to a certain degree. info:eu-repo/classification/ddc/570 ddc:570
12	Environmentally Relevant Concentration of Bisphenol S Shows Slight Effects on SIHUMIx Schäpe, Stephanie Serena, Krause, Jannike Lea, Masanetz, Rebecca Katharina, Riesbeck, Sarah, Starke, Robert, Rolle-Kampczyk, Ulrike, Eberlein, Christian, Heipieper, Hermann-Josef, Herberth, Gunda, von Bergen, Martin, Jehmlich, Nico 20 April 2023 (has links) Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of polycarbonate plastics and epoxy resins and thus can be found in various plastic products and thermal papers. The microbiota disrupting effect of BPS on the community structure of the microbiome has already been reported, but little is known on how BPS affects bacterial activity and function. To analyze these effects, we cultivated the simplified human intestinal microbiota (SIHUMIx) in bioreactors at a concentration of 45 µM BPS. By determining biomass, growth of SIHUMIx was followed but no differences during BPS exposure were observed. To validate if the membrane composition was affected, fatty acid methyl esters (FAMEs) profiles were compared. Changes in the individual membrane fatty acid composition could not been described; however, the saturation level of the membranes slightly increased during BPS exposure. By applying targeted metabolomics to quantify short-chain fatty acids (SCFA), it was shown that the activity of SIHUMIx was unaffected. Metaproteomics revealed temporal effect on the community structure and function, showing that BPS has minor effects on the structure or functionality of SIHUMIx. info:eu-repo/classification/ddc/570 ddc:570
13	Microbe-Environment Interactions in Arctic and Subarctic Systems Zayed, Ahmed Abdelfattah 30 September 2019 (has links) No description available. Biogeochemistry Bioinformatics Biology Biological Oceanography Climate Change Ecology Environmental Science Geobiology Microbiology Oceanography Soil Sciences Statistics Virology viruses microbial expression metagenomics metaproteomics community ecology population ecology community expression marine biology peat carbon degradation permafrost climate change diversity gradients Profile Hidden Markov Models

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