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The Simplified Human Intestinal Microbiota (SIHUMIx) Shows High Structural and Functional Resistance against Changing Transit Times in In Vitro BioreactorsSchäpe, Stephanie Serena, Krause, Jannike Lea, Engelmann, Beatrice, Fritz-Wallace, Katarina, Schattenberg, Florian, Liu, Zishu, Müller, Susann, Jehmlich, Nico, Rolle-Kampczyk, Ulrike, Herberth, Gunda, von Bergen, Martin 11 April 2023 (has links)
Many functions in host–microbiota interactions are potentially influenced by intestinal
transit times, but little is known about the effects of altered transition times on the composition
and functionality of gut microbiota. To analyze these effects, we cultivated the model community
SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the
community structure and function. After five days of continuous cultivation, we investigated the
influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community,
we applied flow cytometric fingerprinting and revealed changes in the community structure of
SIHUMIx during the change of TT, which were not associated with changes in species abundances.
For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found,
along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid
metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and
isovalerate concentrations. Specifically, B. thetaiotaomicron was identified to be affected in terms of
butyrate metabolism. However, communities could recover to the original state afterward. This study
shows that SIHUMIx showed high structural stability when TT changed—even four-fold. Resistance
values remained high, which suggests that TTs did not interfere with the structure of the community
to a certain degree.
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Environmentally Relevant Concentration of Bisphenol S Shows Slight Effects on SIHUMIxSchäpe, Stephanie Serena, Krause, Jannike Lea, Masanetz, Rebecca Katharina, Riesbeck, Sarah, Starke, Robert, Rolle-Kampczyk, Ulrike, Eberlein, Christian, Heipieper, Hermann-Josef, Herberth, Gunda, von Bergen, Martin, Jehmlich, Nico 20 April 2023 (has links)
Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of polycarbonate plastics and epoxy resins and thus can be found in various plastic products and thermal papers. The microbiota disrupting effect of BPS on the community structure of the microbiome has already been reported, but little is known on how BPS affects bacterial activity and function. To analyze these effects, we cultivated the simplified human intestinal microbiota (SIHUMIx) in bioreactors at a concentration of 45 µM BPS. By determining biomass, growth of SIHUMIx was followed but no differences during BPS exposure were observed. To validate if the membrane composition was affected, fatty acid methyl esters (FAMEs) profiles were compared. Changes in the individual membrane fatty acid composition could not been described; however, the saturation level of the membranes slightly increased during BPS exposure. By applying targeted metabolomics to quantify short-chain fatty acids (SCFA), it was shown that the activity of SIHUMIx was unaffected. Metaproteomics revealed temporal effect on the community structure and function, showing that BPS has minor effects on the structure or functionality of SIHUMIx.
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Microbe-Environment Interactions in Arctic and Subarctic SystemsZayed, Ahmed Abdelfattah 30 September 2019 (has links)
No description available.
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