Degradation of permafrost in peatlands can convert forested peat plateaus to inundated collapse bogs. Due to increased unfrozen soil carbon stocks and more saturated conditions, collapse bogs can potentially be large emitters of methane. Using a network of bubble traps permanently installed in peat, I tested several hypotheses about controls on ebullition in collapse bogs with varying time since thaw in interior Alaska. Ebullition increased during the growing season, likely due to increased substrate availability and warmer soils. Bubbles were found primarily in shallow peat layers, and were dominated by modern carbon. Ebullition hot spots were associated with high sedge density throughout the collapse sites. Episodic ebullition occurred during atmospheric pressure changes. Overall, my study demonstrated that permafrost thaw in peatlands will result in methane emissions through ebullition that include both young and old carbon, contradictory to the generally accepted paradigm; that ebullition in peatlands is solely a surface process.

No description available.

No description available.

Field and laboratory studies were performed to estimate fluxes of the a-ace gases nitrogen monoxide (NO), nitrous oxide (N2O), and methane (CH4) in an organic soil, to determine the microbial processes involved, and to assess how soil water and nitrogen controlled flux rates. Metabolic inhibitors showed microbial nitrification to be the major NO source, regardless of the soil moisture content. Nitrification also produced N 2O, but denitrification losses of this gas from flooded, anaerobic soil were much higher. Up to 26% of nitrified N was converted to NO, but most of this (95%) was consumed microbially before it could escape across the soil surface. The NO-consuming process appeared to be co-oxidation by soil heterotrophs, not coupled to energy production. Organic matter content and CO2 production were therefore good predictors of NO oxidation rates across soil types, and NO oxidation was stimulated by manure addition. / Soil water and nitrogen had nonlinear effects on trace gas fluxes, acting on both production and consumption. Kinetic analysis showed that nitrate was a weak noncompetitive inhibitor, but ammonium a strong competitive inhibitor of soil CH4 oxidation at field fertilization rates. However, spatial and temporal factors complicated fertilization effects on CH4 oxidation in situ. Ammonium was immobilized in surface soil and rapidly nitrified, limiting its inhibitory effect on CH 4 oxidation. Fertilizer N stimulated nitrification and denitrification and therefore gaseous N-oxide production, but other, unexpected fertilizer effects were also observed. Ammonium fertilizer decreased NO oxidation rates. Nitrate and other salts stimulated NO and N2O losses during nitrification, an effect apparently related to soil nitrite accumulation. / The controls exerted on trace gas fluxes by soil water were mediated primarily through diffusion rates. Oxygen diffusion controlled the balance of anaerobic (methanogenesis and denitrification) versus aerobic (CH 4 oxidation and nitrification) processes. Soil moisture content also controlled the diffusion rate of atmospheric CH4 to soil methanotrophs, and the escape of gaseous N-oxides from production sites across the soil surface.

A study was conducted to determine if sainfoin, a condensed tannin (CT) containing legume, fed to beef cattle as hay or silage during a western Canadian winter would result in a reduction in methane (CH4) emissions without compromising animal performance. Forty yearling beef steers were fed four diets in a factorial design consisting of two legume forage species (sainfoin or alfalfa) and two preservation methods (silage or hay) over 15 weeks (wks). For each sample wk, animal weight, 24-h CH4 expiration and rumen fluid samples were obtained. Rumen methanogens were characterized using terminal restriction fragment length polymorphisms analysis. Specific bacteria were quantified with real-time polymerase chain reaction analysis. Sainfoin silage (SS) and sainfoin hay (SH) contained 11.9 and 10.5 mg g-1 of CT respectively and supported an acceptable growth rate for backgrounding steers. A decline (P < 0.05) in enteric CH4 formation could only be detected from SH-fed animals compared to alfalfa hay (AH) fed animals when CH4 was expressed as L d-1 or L kg BW-1. The rumen archaeal community structure of experimental animals remained stable regardless of diet type or sample wk. Structural carbohydrate-fermenting bacteria were suppressed in silage diets. Methanogens were less abundant in the rumen fluid samples of steers fed SS but not SH.

Carbon nanotubes present enormous potential for future nanoelectronic applications. This study details one method for producing such nanotubes via chemical vapor deposition (CVD) of methane gas at high temperatures. This method represents the best known way to selectively place nanotubes, as will be needed for complex electronic structures. Various growth conditions are manipulated and the effects on the resulting nanotubes are recorded.

No description available.

No description available.

The purpose of the study was to examine how forest harvesting affects the accumulation of carbon dioxide (CO2) and methane (CH4) under the ice-cover. Dissolved organic carbon (DOC) was hypothesized to increases post-harvest, this supplementation would in turn lead to an increased accumulation of CO2 and CH4 under the ice-cover. Four small boreal lakes, (two treatment lakes, two reference lakes) were sampled once a year during winter, under a three-year period (2012-2014). The sampling was performed using a headspace equilibration technique. During the three study years (2012-2014), the CO2 concentration increased in both the reference lakes and the harvested lakes (percentage increase; 18 % and 89 % respectively). The mean CH4-concentration in the reference lakes increased during the years 2012-2014, (2, 6, 7.5 μM). While the concentrations in the harvested lakes showed a slightly different pattern, with a peak during year 2013 which latter decreased until year 2014, (6, 12.5, 8.5 μM). The result of this study suggests that harvest in the catchment area increases the accumulation of CO2 and possible CH4 under the ice. Inflow of supersaturated surface water was found to be a more important source to the increased accumulation under ice than DOC, contradicting the previous hypothesis that DOC would be a more important factor controlling the CO2 and CH4 production. Moreover this study indicates that the dimension of the edge-zone left in connection to the inlets can be of crucial importance, possible reducing the amount of supersaturated surface water reaching the lakes.

A study was conducted to determine if sainfoin, a condensed tannin (CT) containing legume, fed to beef cattle as hay or silage during a western Canadian winter would result in a reduction in methane (CH4) emissions without compromising animal performance. Forty yearling beef steers were fed four diets in a factorial design consisting of two legume forage species (sainfoin or alfalfa) and two preservation methods (silage or hay) over 15 weeks (wks). For each sample wk, animal weight, 24-h CH4 expiration and rumen fluid samples were obtained. Rumen methanogens were characterized using terminal restriction fragment length polymorphisms analysis. Specific bacteria were quantified with real-time polymerase chain reaction analysis. Sainfoin silage (SS) and sainfoin hay (SH) contained 11.9 and 10.5 mg g-1 of CT respectively and supported an acceptable growth rate for backgrounding steers. A decline (P < 0.05) in enteric CH4 formation could only be detected from SH-fed animals compared to alfalfa hay (AH) fed animals when CH4 was expressed as L d-1 or L kg BW-1. The rumen archaeal community structure of experimental animals remained stable regardless of diet type or sample wk. Structural carbohydrate-fermenting bacteria were suppressed in silage diets. Methanogens were less abundant in the rumen fluid samples of steers fed SS but not SH.