Made available in DSpace on 2016-04-28T20:17:26Z (GMT). No. of bitstreams: 1 2006-Felipe Lopes Campos.pdf: 553352 bytes, checksum: e4d71e5d911a1305af8111fa2c150966 (MD5) Previous issue date: 2006-03-16 / The aim of this survey was the isolation of the Cryptococcus neoformans yeast from cats and dogs central nervous system in Rio de Janeiro City. Periodic collections were made from brain samples from April 2004 to July 2005 at the rabies diagnosis laboratory in the Jorge Vaitsman Veterinary Medicine Municipal Institute in Mangueira, RJ. According to the results, of the 201 clinical samples, we found four positives samples; one of these was positive for C. laurentii, three were positive for Cryptococcus neoformans; of which two were neoformans variety and one was gattii variety. Secondarily we compared with strains from the ICB-USP, previously isolated and identified as for urease and fenol-oxidase production in qualitative character, in the same way that the potential of virulence demonstrated to fosfolipase and protease production. Killer biotyping used in all isolates showed a predominance of biotype II to neoformans variety and biotype VIII to gattii variety. All isolates showed protease and fosfolipase production in varied degrees. lipase / Objetivando o isolamento da levedura Cryptococcus neoformans do sistema nervoso central de c?es e gatos no Munic?pio do Rio de Janeiro, procedeu-se ?s coletas peri?dicas, no per?odo de abril de 2004 a julho de 2005, de amostras de c?rebro, no Laborat?rio de Diagn?stico de Raiva do Instituto Municipal de Medicina Veterin?ria Jorge Vaitsman no bairro da Mangueira, Rio de Janeiro. Das 201 amostras obteve-se um total de quatro positivas para o g?nero; sendo uma de Cryptococcus laurentii e tr?s de C. neoformans, das quais duas revelaram-se pertencentes ? variedade neoformans e uma ? variedade gattii. Secundariamente, confrontamos estas com amostras obtidas junto ao ICB-USP, previamente isoladas e identificadas, quanto ao potencial de virul?ncia espelhado pela produ??o de fosfolipase e de protease. A biotipagem Killer empregada em todos os isolados demonstrou uma predomin?ncia do biotipo II para a variedade neoformans e biotipo VIII para a variedade gattii. Todos os isolados apresentaram-se produtores de protease e de fosfolipase, em diferentes graus.