Made available in DSpace on 2016-08-10T10:55:17Z (GMT). No. of bitstreams: 1 JAIRO FIGUEIREDO JUNIOR.pdf: 1092705 bytes, checksum: e53e9da5866dc4681e74d326402f8e37 (MD5) Previous issue date: 2007-02-26 / Toxoplasma gondii is an obligatory intracellular parasite, capable of infecting a large variety of intermediate hosts including humans. In the domiciliary environment, the infected final hosts (cats) spread the oocists by their excrements, which quickly reach maturity in the ground and become infectants. Therefore, toxoplasmosis is acquired by humans through the ingestion of oocists found in the domiciliary environment or by eating insufficiently cooked meat, infected with cysts. The antitoxoplasma antibodies presence can be determined by several serologic reactions, using different serologic markers to distinguish recent infection from toxoplasma-illness. In this work, we carried out serologic assays, defined a curve by the digital recognition of color using the Toxo IgG Digital Microscopical Immunofluorescence. The curve was related with the results in UI/ml read by the quantitative determination of IgG antibodies for the Toxoplasma gondii of Toxo in the G VIDAS® system. The existing correlation between sera in UI/ml and their values found through the digitalized green intensity curve was evidenced. In the analysis between the predetermined sera in UI/ml and its read values, did not show differences that could affect the laboratorial diagnosis. The analysis of the behavior of the curves defined by this works, shows that the approach adopted by this work is promising. for the development of a novel method for Immunofluorescence analysis with significant gains in precision and price reduction. The rational use of technologies, even so the rationality is not neutral, implies the election of technologies to be financed and the identification of the conditions or sub-groups where they will have to be used, in the direction to become the system of more efficient health for the objective to protect and to recoup the health of the population. Correlation to the unemployment level social impact in companies affected by the technological modernization progress. The expectation is that it has, through public politics in set with private initiative, action for creation of courses that prepare the workers for the use of these new technologies. / O Toxoplasma gondii é um parasito intracelular obrigatório, capaz de infectar uma grande variedade de hospedeiros intermediários, incluindo o homem. No ambiente domiciliar, os hospedeiros definitivos infectados (gatos) eliminam os oocistos pelas fezes, os quais, rapidamente, atingem maturidade no solo e se tornam infectantes. Portanto, a toxoplasmose é adquirida pelo homem por meio da ingestão de oocistos encontrados no meio ambiente domiciliar ou de carnes infectadas com cistos e insuficientemente cozidas. A ocorrência de anticorpos antitoxoplasma tem sido determinada por diversas reações sorológicas, utilizando-se diferentes marcadores sorológicos que possibilitam distinguir infecção recente de toxoplasma-doença. Neste projeto de pesquisa, realizaram-se ensaios sorológicos em que a curva obtida pelo reconhecimento digital de cor, pela técnica de Imunofluorescência Microscópica Digital para Toxo IgG, foi relacionada com os resultados em UI/mL obtidos pela determinação quantitativa de anticorpos IgG para o Toxoplasma gondii do sistema VIDAS® Toxo G. Ficou evidenciada a correlação existente entre os soros em UI/mL predeterminados e seus valores encontrados através da equação da curva digitalizada da intensidade de verde. Na comparação entre os soros em UI/mL predeterminados e seus valores encontrados, não foram observadas diferenças que pudessem alterar o diagnóstico clínico-laboratorial. A utilização do conhecimento sobre o comportamento das curvas resultantes das interações das leituras visual e digital demonstra que a proposta deste trabalho é promissora para o desenvolvimento de uma nova forma de análise de imunoflourescência, com significativos ganhos de precisão e redução de preços. A racionalidade não é neutra, portanto o uso racional de tecnologias implica a seleção daquelas que devem ser financiadas e a identificação das condições ou subgrupos em que deverão ser utilizadas com o fim de tornar o sistema de saúde mais eficiente para proteger e recuperar a saúde da população. Com relação ao possível desemprego em empresas envolvidas no processo de modernização tecnológica, o que se espera é que, por meio de políticas públicas em conjunto com a iniciativa privada, sejam desenvolvidas ações no sentido de preparar os trabalhadores para o uso dessas novas tecnologias.

Aim of study. The purpose was to investigate and analyse mycotoxicological contamination of concentrated fodder during 2 years and to evaluate the use of chemical conserving CALPRONA NC. Main tasks: 1) To investigate the amounts of aflatoxin B1, zearelenone, T2 and deoxivalenol (DON) mycotoxins in fodder using imunoferment analysis and chromatography (TLC) method’s. 2) To determine minimum and maximum levels of mycotoxins in fodder and compare with the formative’s. 3) Compare levels of mycotoxins in fodder during period of two years. 4) Investigate the influence of chemical conserving CALPRONA NC mycostatical effect on the grain after the harvest. Material and methods: Conservated (CALPRONA NC) and non conservated samples were taken from experimental farm. Quantitative contamination of fungi (cfu – colony forming units) was determined using dilution method and Czapec Dox agar (Курасова, 1971). Level of mycotoxins was determined using IFA (imunoferment analyses) method with commercial set VERATOX®DON, T- 2 toxin, Zearalenone. Also the TLC method was applied using silicon gel plates (methology of Romer Lab.) Diffusion of mycotoxins in fodder during year 2003- 2004 was determined using processing of facts with “R” statistical pack. Results and discussion: 1) Probe fodder is contaminated with fungi and their toxins: DON, zearalenone, T- 2 and aflatoxin B1. 2) Highest levels of mycotoxins in concentrated fodder (mg/kg or ppm) are DON (0.095 ppm), comparing to zearalenone (ZON) and... [to full text]