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RELAÇÃO DE RESULTADOS SOROLÓGICOS DE ENSAIO IMUNOENZIMÁTICO (ELISA) COM A TÉCNICA DE IMUNOFLUORESCÊNCIA MICROSCÓPICA DIGITAL PARA PESQUISA DE ANTIGLOBULINA IGG DE TOXOPLASMA GONDIIFigueiredo Junior, Jairo 26 February 2007 (has links)
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Previous issue date: 2007-02-26 / Toxoplasma gondii is an obligatory intracellular parasite, capable of infecting a large
variety of intermediate hosts including humans. In the domiciliary environment, the
infected final hosts (cats) spread the oocists by their excrements, which quickly reach
maturity in the ground and become infectants. Therefore, toxoplasmosis is acquired by
humans through the ingestion of oocists found in the domiciliary environment or by
eating insufficiently cooked meat, infected with cysts. The antitoxoplasma antibodies
presence can be determined by several serologic reactions, using different serologic
markers to distinguish recent infection from toxoplasma-illness. In this work, we carried
out serologic assays, defined a curve by the digital recognition of color using the Toxo
IgG Digital Microscopical Immunofluorescence. The curve was related with the results
in UI/ml read by the quantitative determination of IgG antibodies for the Toxoplasma
gondii of Toxo in the G VIDAS® system. The existing correlation between sera in
UI/ml and their values found through the digitalized green intensity curve was
evidenced. In the analysis between the predetermined sera in UI/ml and its read values,
did not show differences that could affect the laboratorial diagnosis. The analysis of the
behavior of the curves defined by this works, shows that the approach adopted by this
work is promising. for the development of a novel method for Immunofluorescence
analysis with significant gains in precision and price reduction. The rational use of
technologies, even so the rationality is not neutral, implies the election of technologies
to be financed and the identification of the conditions or sub-groups where they will
have to be used, in the direction to become the system of more efficient health for the
objective to protect and to recoup the health of the population. Correlation to the
unemployment level social impact in companies affected by the technological
modernization progress. The expectation is that it has, through public politics in set with
private initiative, action for creation of courses that prepare the workers for the use of
these new technologies. / O Toxoplasma gondii é um parasito intracelular obrigatório, capaz de infectar
uma grande variedade de hospedeiros intermediários, incluindo o homem. No ambiente
domiciliar, os hospedeiros definitivos infectados (gatos) eliminam os oocistos pelas
fezes, os quais, rapidamente, atingem maturidade no solo e se tornam infectantes.
Portanto, a toxoplasmose é adquirida pelo homem por meio da ingestão de oocistos
encontrados no meio ambiente domiciliar ou de carnes infectadas com cistos e
insuficientemente cozidas. A ocorrência de anticorpos antitoxoplasma tem sido
determinada por diversas reações sorológicas, utilizando-se diferentes marcadores
sorológicos que possibilitam distinguir infecção recente de toxoplasma-doença. Neste
projeto de pesquisa, realizaram-se ensaios sorológicos em que a curva obtida pelo
reconhecimento digital de cor, pela técnica de Imunofluorescência Microscópica Digital
para Toxo IgG, foi relacionada com os resultados em UI/mL obtidos pela determinação
quantitativa de anticorpos IgG para o Toxoplasma gondii do sistema VIDAS® Toxo G.
Ficou evidenciada a correlação existente entre os soros em UI/mL predeterminados e
seus valores encontrados através da equação da curva digitalizada da intensidade de
verde. Na comparação entre os soros em UI/mL predeterminados e seus valores
encontrados, não foram observadas diferenças que pudessem alterar o diagnóstico
clínico-laboratorial. A utilização do conhecimento sobre o comportamento das curvas
resultantes das interações das leituras visual e digital demonstra que a proposta deste
trabalho é promissora para o desenvolvimento de uma nova forma de análise de
imunoflourescência, com significativos ganhos de precisão e redução de preços. A
racionalidade não é neutra, portanto o uso racional de tecnologias implica a seleção
daquelas que devem ser financiadas e a identificação das condições ou subgrupos em
que deverão ser utilizadas com o fim de tornar o sistema de saúde mais eficiente para
proteger e recuperar a saúde da população. Com relação ao possível desemprego em
empresas envolvidas no processo de modernização tecnológica, o que se espera é que,
por meio de políticas públicas em conjunto com a iniciativa privada, sejam
desenvolvidas ações no sentido de preparar os trabalhadores para o uso dessas novas
tecnologias.
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Mikotoksinų kaupimosi koncentruotuose pašaruose tyrimų analizė / Analysis of mycotoxins accumuliation in concentrated fodderLebedevaitė, Erika 08 April 2005 (has links)
Aim of study. The purpose was to investigate and analyse mycotoxicological contamination of concentrated fodder during 2 years and to evaluate the use of chemical conserving CALPRONA NC.
Main tasks:
1) To investigate the amounts of aflatoxin B1, zearelenone, T2 and deoxivalenol (DON) mycotoxins in fodder using imunoferment analysis and chromatography (TLC) method’s.
2) To determine minimum and maximum levels of mycotoxins in fodder and compare with the formative’s.
3) Compare levels of mycotoxins in fodder during period of two years.
4) Investigate the influence of chemical conserving CALPRONA NC mycostatical effect on the grain after the harvest.
Material and methods:
Conservated (CALPRONA NC) and non conservated samples were taken from experimental farm.
Quantitative contamination of fungi (cfu – colony forming units) was determined using dilution method and Czapec Dox agar (Курасова, 1971).
Level of mycotoxins was determined using IFA (imunoferment analyses) method with commercial set VERATOX®DON, T- 2 toxin, Zearalenone. Also the TLC method was applied using silicon gel plates (methology of Romer Lab.)
Diffusion of mycotoxins in fodder during year 2003- 2004 was determined using processing of facts with “R” statistical pack.
Results and discussion:
1) Probe fodder is contaminated with fungi and their toxins: DON, zearalenone, T- 2 and aflatoxin B1.
2) Highest levels of mycotoxins in concentrated fodder (mg/kg or ppm) are DON (0.095 ppm), comparing to zearalenone (ZON) and... [to full text]
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