This study assesses the role of crossing locations and light conditions in pedestrian injury severity through a multivariate regression analysis to control for many other factors that also may influence pedestrian injury severity. Crossing locations include midblock and intersections, and light conditions include daylight, dark with street lighting, and dark without street lighting. The study formulates a theoretical framework on the determinants of pedestrian injury severity, and specifies an empirical model accordingly. An ordered probit model is then applied to the KABCO severity scale of pedestrian injuries which occurred while attempting street crossing in the years 1986 to 2003 in Florida. In terms of crossing locations, the probability of a pedestrian dying when struck by a vehicle, is higher at midblock locations than at intersections for any light condition. In fact, the odds of sustaining a fatal injury is 49 percent lower at intersections than at midblock locations under daylight conditions, 24 percent lower under dark with street lighting conditions, and 5 percent lower under dark without street lighting conditions. Relative to dark conditions without street lighting, daylight reduces the odds of a fatal injury by 75 percent at midblock locations and by 83 percent at intersections, while street lighting reduces the odds by 42 percent at midblock locations and by 54 percent at intersections.