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The content of this thesis is to analyze the product portfolio of the company ECS Invention spol. s r. o. and a proposal for its innovation. Recommendations related to the product portfolio are included as well. The thesis is divided into two parts. The theoreticalpart is focused on theoretical and methodological background of strategic marketing and innovations. The practical part deals with application of the theoretical knowledge into practice.

Anacampserotaceae pertence à ordem Caryophyllales, subordem Portulacineae, atualmente constituída por três gêneros (Anacampseros L., Grahamia Gill ex Hook e Talinopsis A. Gray) antes pertencentes à família Portulacaceae. Os representantes de Anacampserotaceae apresentam estruturas axilares lignificadas muito características, como tricomas, escamas (scales) e cerdas (bristles) e também apresentam células do tipo WBT que ocorre somente em mais outras duas famílias de Caryophyllales, Aizoaceae e Cactaceae. Os caracteres axilares são muito informativos para o clado ACPT (Anacampserotaceae, Cactaceae, Portulacaceae e Talinaceae) e vêm revelando importantes dados aos estudos filogenéticos da subordem Portulacineae, no entanto, há uma controvérsia quanto a origem ontogenética das estruturas axilares de Anacampserotaceae, o que pode levar a interpretações equivocadas das relações filogenéticas do grupo. Diante disso, este trabalho realizou um estudo ontogenético das estruturas presentes na região da axila foliar de oito espécies de Anacampserotaceae e analisou as WBTs do caule e de folhas quanto seu tipo de espessamento e medidas quantitativas de seis espécies. Os resultados apontam a 1) origem estipular das escamas e não protodérmica, como mencionado na literatura; 2) primeiro relato de profilos na família e 3) WBTs ocorrem em Anacampseros e estão ausente em Talinopsis, grupo considerado basal na família. / Anacampserotaceae belongs to the order Caryophyllales, suborder Portulacineae, currently consists of three genus (Anacampseros L., Grahamia Gill ex Hook and Talinopsis A. Gray) formerly circumscribed to the family Portulacaceae. Anacampserotaceae\'s representative presents lignified axillary structures very distinctive, like trichomes, scales and bristles and also WBT cells that is presented only by two other families of Caryophyllales, Aizoaceae e Cactaceae. The axillary characters are very informative for the ACPT clade (Anacampserotaceae, Cactaceae, Portulacaceae and Talinaceae) and has been revealing important data for the phylogenetic studies on the Portulacineae suborder, nevertheless, there is a controversy regarding the Anacampserotaceae axillary structures ontogenetic origin, what may lead to misguided interpretations for the ACPT phylogenetic relationships. Before it, this research have accomplished the ontogenetic study of the structures present on the leaf axil of eight Anacampserotaceae species and examined stem and leaf WBTs, as for its secondary thickening type and quantitative measurements of six species. The results points 1) that at least one structure (scale) has leaf origin instead of protodermic origin, as stated by some authors; 2) first report of prophylls for the family and 3) WBTs occur in Anacampseros but they are absent in Talinopsis, a basal group of Anacampserotaceae.

No description available.

Unlike many other international instruments, accession to the WTO does not simply require the commitment of the government to sign and ratify the multilateral agreements. A country needs to make considerable legislative and administrative changes to comply with the standards of the WTO and its members to finalize the negotiation for accession. For governments with impure free market economy policy the challenges amplify. The government of Ethiopia publicly pronounces its adherence to the ideology of the developmental state. On the other hand the nucleus of WTO principles is progressive trade liberalization. Therefore, this dissertation tries to provide some reflection on the paradox created as a result of the divergence in priority between WTO principles and developmental state in the context of Ethiopian desire to join the organization. The research is an interdisciplinary work. The issues that will be discussed are not purely legal in their nature. They have legal, political and economic dimensions. And the main focus of the paper is on trade in services and foreign investment negotiation aspect of the accession. Furthermore the objective of the dissertation is to give some insight for policy makers about the challenges and opportunities that „Developmental State‟ ideology will pose in the accession process of Ethiopia to the WTO. The research is divided into five chapters. Chapter one gives introductory remarks about the concept of the developmental state and accession to the WTO. The limitations of the WTO accession process and an overview of the features of developmental state in the world and particularly in Ethiopia are also briefly discussed in the chapter. The origin and concept of developmental state in the world, in Africa and Ethiopia is discussed in some detail under chapter two. The chapter also tries to show the impact of developmental state policies in the laws of the country that are going to be deliberated in the process of accession. Chapter three is about accession to the WTO. In this chapter the requirements, benefits, challenges and procedures of accession are dealt in depth. The writer debates and tries to show the fact that the system is slowly shifting from rule based negotiation to power and precedent based negotiation. By analyzing the laws of Ethiopia that are inspired by the principles of developmental state against the legal and precedent requirements to join the WTO, I tried to correlate the findings of chapter two and three in chapter four. Specific strategies and advises on how to move the negotiation forward on certain areas are also outlined in this chapter. Finally, conclusion and my summarized recommendations are placed under chapter five. / Economics / LL.M (with specialization in International Economic Law)

Unlike many other international instruments, accession to the WTO does not simply require the commitment of the government to sign and ratify the multilateral agreements. A country needs to make considerable legislative and administrative changes to comply with the standards of the WTO and its members to finalize the negotiation for accession. For governments with impure free market economy policy the challenges amplify. The government of Ethiopia publicly pronounces its adherence to the ideology of the developmental state. On the other hand the nucleus of WTO principles is progressive trade liberalization. Therefore, this dissertation tries to provide some reflection on the paradox created as a result of the divergence in priority between WTO principles and developmental state in the context of Ethiopian desire to join the organization. The research is an interdisciplinary work. The issues that will be discussed are not purely legal in their nature. They have legal, political and economic dimensions. And the main focus of the paper is on trade in services and foreign investment negotiation aspect of the accession. Furthermore the objective of the dissertation is to give some insight for policy makers about the challenges and opportunities that „Developmental State‟ ideology will pose in the accession process of Ethiopia to the WTO. The research is divided into five chapters. Chapter one gives introductory remarks about the concept of the developmental state and accession to the WTO. The limitations of the WTO accession process and an overview of the features of developmental state in the world and particularly in Ethiopia are also briefly discussed in the chapter. The origin and concept of developmental state in the world, in Africa and Ethiopia is discussed in some detail under chapter two. The chapter also tries to show the impact of developmental state policies in the laws of the country that are going to be deliberated in the process of accession. Chapter three is about accession to the WTO. In this chapter the requirements, benefits, challenges and procedures of accession are dealt in depth. The writer debates and tries to show the fact that the system is slowly shifting from rule based negotiation to power and precedent based negotiation. By analyzing the laws of Ethiopia that are inspired by the principles of developmental state against the legal and precedent requirements to join the WTO, I tried to correlate the findings of chapter two and three in chapter four. Specific strategies and advises on how to move the negotiation forward on certain areas are also outlined in this chapter. Finally, conclusion and my summarized recommendations are placed under chapter five. / Economics / LL. M. (with specialization in International Economic Law)

The 3' terminal stem loop of the WNV minus-strand [WNV3'(-) SL] RNA was previously shown to bind the cell protein, T-cell intracellular antigen-1 (TIA-1), and the related protein, TIAR. These two proteins are known to bind AU-rich sequences in the 3' UTRs of some cellular mRNAs. AU stretches are located in three single-stranded loops (L1, L2, and L3) of the WNV3'(-) SL RNA. The RNA binding activity of both proteins was reduced when L1 or L2, but not L3, AU sequences were deleted or substituted with Cs. Deletion or substitution with Cs of the entire AU-rich sequence in either L1 or L2 in a WNV infectious clone was lethal for the virus while mutation of some of these nt decreased the efficiency of virus replication. Mutant viral RNAs with small plaque or lethal phenotypes had similar translational efficiencies to wildtype RNA, but showed decreased levels of plus-strand RNA synthesis. These results correlated well with the efficiency of TIA-1 and/or TIAR binding in in vitro assays. In normal cells, TIA-1 and TIAR are evenly distributed in the cytoplasm and nucleus. Between 6 and 24 hr after WNV infection, TIAR concentrated in the perinuclear region and TIA-1 localization to this region began by 24 hr. Similar observations were made in DV2 infected cells but at later times after infection. In infected cells, both proteins colocalized with dsRNA, a marker for viral replication complexes, and with viral non-structural proteins. Anti-TIAR or anti-TIA-1 antibody coimmunoprecipitated viral NS3 and possibly other viral nonstructural proteins. In response to different types stress, TIA-1 and TIAR recruit cell mRNA poly(A)+ into cytoplasmic stress granules (SG) leading to general translational arrest in these cells. SG were not induced by flavivirus infection and cells became increasingly resistant to arsenite induction of SG with time after infection. Processing Body (PB) assembly was also decreased beginning at 24 hr. These data suggest that the sequestration of first TIAR and then TIA-1 via their interaction with viral components in flavivirus infected cells inhibits SG formation and prevents the shutoff of host translation.

No description available.