Despite over a century of research, malaria parasites (Plasmodium) still remain a major cause of mortality and morbidity worldwide. In recent years, the application of theoretical principles from ecology and evolutionary biology to the study of these parasites has started to provide insight into variety of fundamental subjects from the evolution of virulence to the facultative strategies (i.e. phenotypic plasticity) that parasites use to maximize their transmission. It is now becoming increasingly clear that to understand and predict population level patterns of virulence and transmission, the processes that occur at the between-host level must be studied in light of the interactions that happen within hosts (between parasites and between parasites and hosts). In this thesis I combine concepts from evolutionary biology and ecology with tools from molecular and cellular biology and evolutionary genetics, which allow me to study rodent malaria parasites at both evolutionary and ecological timescales. The work I present in this thesis has the following four components: 1. Phylogenetics (chapter 2): I applied recently developed phylogenetic methods to a large DNA sequence dataset that I generated, to provide a better understanding of the phylogeny of rodent malaria parasites and investigate how selection has shaped their genomes. I show that all rodent malaria subspecies can be considered species, provide the first time line for the evolution of this group of parasites and demonstrate that most loci are under purifying selection. 2. Hybridization and reproductive isolation (chapter 3): I show that hybridization between two rodent malaria parasites (P. berghei and P. yoelii) can occur, but only occurs at high levels when one of two proteins (P230 or P48/45) is absent from the surface of female gametes, which indicates that these proteins are involved in gamete recognition. I find that P230, P48/45 and P47 (a possible interaction partner) are evolving under positive selection, a feature often observed in gamete recognition proteins of other taxa. Finally, I show that the fertilization success of P. berghei is reduced in the presence of P. yoelii, but not vice-versa, which indicates asymmetric reproductive interference. 3. Sex allocation (chapter 4): I carry the first test of sex allocation’s assumption that immunity impacts on the fertility of Plasmodium male gametocytes/gametes more than on the fertility of females. I show that while the fertility of both males and females is equally affected, males are affected during gametogenesis and females are mostly affected through gamete dysfunction (i.e. gametes can mate but zygotes fail to develop), which is in agreement with the assumptions of theory. In collaboration, I incorporate these effects into sex allocation theory and predict that malaria parasites can minimize the effects of factors that kill gametocytes/gametes by adjusting their sex ratios. On the other hand sex ratio adjustment cannot compensate for gamete dysfunction or zygote death. These results have applied implications for transmission-blocking vaccines. 4. Infection dynamics of mixed-species infections (chapter 5): I develop a series of experiments to test how a focal parasite species (P. yoelii) is affected by competition with heterospecifics (P. chabaudi) and how the interaction between the two species is mediated by immunity and resource availability. I show that P. chabaudi can boost P. yoelii above its single species level (i.e. facilitation) and that this is mediated by resource availability. On the other hand, P. yoelii’s performance can also be hindered in mice that were exposed to a P. chabaudi infection. My results also reveal that host mortality is exacerbated in mixed-species infections of naïve mice, which may be due to an inability of the host to achieve the right balance between the production and the destruction of red blood cells, when dealing with a mixed-species infection. The work I present here tackles fundamental questions concerning the transmission biology and the within-host interactions of malaria parasites The results presented demonstrate the importance of interactions between hosts and parasites and between different parasite species (at the molecular and the whole organism levels) for determining the outcome of transmission, virulence and within-host parasite performance.

The question of group-living in animals has attracted a lot of research attention. Today, we have a nuanced understanding of ultimate explanations and proximate mechanisms for group-living, as well as the genetic basis for seemingly puzzling aspects of sociality such as altruism. However, our current view of sociality has been obtained almost entirely in an intraspecific context, i.e. based on groups consisting of individuals of a single species. On the other hand, heterospecific sociality, i.e. groups formed by individuals of multiple species, has been largely ignored in group-living theory and empirical research. This is despite the fact that heterospecific sociality is widely prevalent in nature and is known to occur in many different contexts, in a variety of taxa and in both marine and terrestrial habitats. My dissertation focuses on one of the best-known examples of heterospecific sociality, namely heterospecific foraging associations of terrestrial insectivorous birds (commonly called mixed-species flocks; flocks hereon). Flocks are found all over the world, in a variety of habitats, and include a unique suite of species in each area. Although flocks are the best-studied among different types of heterospecific associations known, most of the research has been descriptive. In other words, while we have descriptions of the richness, size and composition of flocks from many areas, we know little about why and how flocks form. Therefore, one broad objective of this dissertation was to better understand the causes of flock formation. Birds are thought to join flocks either to obtain direct foraging benefits or better protection from predators. I used different approaches to understand which of these two reasons is likely to be more important. Additionally, to situate heterospecific sociality within sociality in general, I also examined whether the mechanisms underlying flocks are similar to single-species groups or unique to a mixed-species condition. In other words, are flocks a different route to obtain the same benefits as single-species groups, or a route to obtain benefits that conspecifics cannot provide? In the previous section, I described why understanding flocks is important from the point of view of sociality. Flocks are also important for the consequences they might have on community organization. While each flock is a collection of interacting individuals of different species, populations of species are linked in a network of interactions across multiple flocks in an area. The theoretical framework guiding our understanding of community organization is currently dominated by the idea of interspecific competition. Mutualisms or positive interactions, if any, are only expected to occur between members of different trophic levels. Only recently has the need to incorporate positive interactions between members of the same trophic level into ecological theory been emphasized. Flocks and other heterospecific associations present an important example of within-trophic level positive interactions. Therefore, the second broad objective of this dissertation was to examine the consequences of flock formation on community organization. The objectives described above were addressed using multiple approaches in this dissertation. “Why” questions in ecology and evolution are generally tackled using manipulative experiments. Given that experiments are neither feasible nor ethical in the case of multispecies flocks in the wild, I addressed the “why” question by focusing on “who” instead. To understand “why” flocks form, I examined what kinds of species participate in flocks, who associates with whom, and who provides and who receives the benefits. This dissertation uses a combination of methods and data, including questions addressed at a global scale using descriptive information on flocks available from across the world. For other questions that were based on entirely new approaches developed in this dissertation, data were collected in a field site in the Western Ghats. In general, given the two broad objectives of this dissertation, the approaches I used were drawn from both behavioural and community ecology. The dissertation is organized in the following way: Chapter 1: General Introduction The first chapter provides the background to the two broad objectives of this thesis, namely understanding the causes and consequences of heterospecific foraging associations in terrestrial bird communities. Chapter 2 (published in The American Naturalist) Based on a global dataset (55 presence-absence matrices from 24 locations in multiple continents) on the composition of flocks, I asked if flocks largely consist of ecologically similar or dissimilar species. Using null models and randomization tests followed by meta-analysis, I found that the association strength of species in flocks was strongly related to similarity in body size and foraging behaviour, and higher for congeneric compared with non-congeneric species pairs. In other words, flocks seem to consist largely of similar species. Extending group-living and social information use theory to a heterospecific context, I discuss potential behavioral mechanisms leading to positive interactions among similar species in flocks as well as ways in which competition costs are reduced. These findings highlight the need to consider positive interactions along with competition when seeking to explain community organisation. Chapter 3 (published in Animal Behaviour) Two kinds of participants are recognized in flocks: those that join other species (‘followers’) and are therefore likely to be the recipients of the benefits of flock participation and those that are joined (‘leaders’). Through comparative analyses, using a large sample of flocks from around the world, I found (1) ‘followers’ tend to be smaller, more insectivorous, and feed in higher strata than matched species that participate in flocks to a lesser extent and (2) ‘leaders’ tend to be cooperative breeders (which are known to have well-developed anti-predatory systems) more often than matched species that are not known to lead flocks. Furthermore, meta-analyses of published results from across the world showed that bird species in terrestrial mixed-species flocks increased foraging rates and reduced vigilance compared to when they were solitary or in conspecific groups. Moreover, the increase in foraging rates was seen only in the case of flock followers and not flock leaders. These findings suggest a role for predation in the evolution of mixed-species flocking. Species that are vulnerable to predation follow species whose vigilance they can exploit. By doing so, they are able to reduce their own vigilance and forage at higher rates. Chapter 4 (to be submitted to Behavioural Ecology and Sociobiology) In this study, conducted in a tropical evergreen forest in the Western Ghats of India, I used intraflock association patterns to generate a community-wide assessment of benefits of flock participation for different species. I assumed that individuals needed to be physically proximate to particular heterospecific individuals within flocks to obtain any direct foraging benefit (flushed prey, kleptoparasitism, copying foraging locations). Alternatively, for all anti-predation benefits, physical proximity to particular heterospecifics is not required, i.e. just being in the flock vicinity will suffice. Therefore, I used the choice of locations within flocks to infer whether individual species are obtaining direct foraging or antipredation benefits. A small subset of the bird community (5/29 species), composed of all members of the sallying guild, showed non-random physical proximity to heterospecifics within flocks. All preferred associates were from non-sallying guilds, suggesting that the sallying species were likely obtaining direct foraging benefits, either in the form of flushed or snatched prey. The majority of species (24/29) chose locations randomly with respect to heterospecifics within flocks, and thus were likely obtaining anti-predation benefits. In summary, my study indicates that direct foraging benefits are important for only a small proportion of species in flocks and therefore that predation is likely to be the main driver of flocking. Chapter 5 (to be submitted to Oecologia) Two types of species – intraspecifically gregarious and sallying species – are thought to play important roles in flocks because studies have shown they attract other flock participants. However, it is not clear why these types of species are attractive, i.e. are they cues for flocks or do they directly provide benefits to other species? It is also not known whether these types are essential for flock formation. In this study, in a tropical evergreen forest in the Western Ghats of India, I used a novel approach to address these questions. Flocks contain anywhere between two and tens of species. My approach focused on the simplest, i.e. two-species, flocks. In two-species flocks, attraction between species must be based on direct benefit to at least one of the species. Therefore, only species combinations that result in benefit to at least one species will occur as two species flocks. Further, by examining leading and following behaviour in these combinations, I could also determine the direction of benefit-flow. Finally, given that all flocks pass through a two-species step, examining which two-species combinations are joined by other species helped understand species roles in flock formation. I found that intraspecifically gregarious species, but not sallying species, were disproportionately represented in two-species flocks, always provided the benefits when present, and that flocks containing them were joined significantly more often. Therefore, intraspecifically gregarious species, but not sallying species, play a role in providing flock benefits and flock formation. Moreover, given that most (7/8) intraspecifically gregarious species in my study site played these roles, the intraspecifically gregarious species guild can be considered an example of a within-trophic level “keystone”. Chapter 6 (“in press” in Oikos) There is a growing recognition of the need to integrate non-trophic interactions into ecological networks for a better understanding of whole-community organization. To achieve this, the first step is to build networks of individual non-trophic interactions. In this study, I analyzed the network of interdependencies among bird species that participated in flocks in an evergreen forest site in the Western Ghats, India. I found that flock networks contain a small core of highly important species that other species are strongly dependent on, a pattern seen in many other biological networks. Further, I found that structural importance of species in the network was strongly correlated to functional importance of species at the individual flock level. Finally, comparisons with flock networks from other Asian forests showed that the same taxonomic groups were important in general, suggesting that species importance was an intrinsic trait and not dependent on local ecological conditions. Hence, given a list of species in an area, it may be possible to predict which ones are likely to be important. Chapter 7: Conclusions In this section, I provide a summary of the main findings of this dissertation, discuss the main conclusions with regard to each of the two broad objectives and finally suggest future lines of investigation to further understand the causes and consequences of flock formation and heterospecific sociality in general. In summary, the work presented in this dissertation provides a picture of how and why flocks form and their likely consequences for community organization. The main driver of flock formation seems to be protection from predators, although a few species do obtain direct foraging benefits. Additionally, given that I found flocks to largely be groupings of similar species, the mechanisms through which benefits are obtained are likely to be similar to those in single-species groups. In other words, flocks are possibly a way for species that are unable to group with conspecifics, to obtain group-living benefits. My finding, that flocks largely consisted of similar species, also questions the stereotyping of interactions between similar species in communities as competitive. Other results from this study demonstrate the importance of intraspecifically gregarious species in flocks. Such species are the main providers of benefits to other flock participants and seem essential for flock formation. Finally, network analysis showed that a small core of species, mainly including species known to provide benefits at the individual flock level, are disproportionately important in the emergent community-level network of interdependencies. Such species are possibly one of the first examples of within-trophic level “keystones” discovered. Apart from the findings, this study also provides a set of new approaches and analytical frameworks that can be used to examine other multispecies foraging groups and heterospecific foraging associations in other contexts.

No description available.

The use of nitrogen (N) fertilizer is generally restricted on mixed species forage systems due to its stimulatory effect on grasses which increases competition with legume species. Reduced legume growth from this competition can compromise forage nutritive value and prospective yields. The controlled-release nature of several enhanced efficiency fertilizer N products holds the potential to improve legume persistence in mixed species pastures while providing supplemental N required by the grass component. The studies contained in this dissertation evaluated the effect of different enhanced efficiency N formulations (ATU, ESN, methylene urea, SuperU, and a 75% ESN: 25% urea blend) and untreated urea on yield, nutritive value, and legume persistence in a ‘Wrangler’ bermudagrass and ‘Durana’ white clover mixture (2014-2016 growing seasons), ‘KY-31’tall fescue and ‘Kenland’ red clover mixture (2015-2016 growing seasons), and ‘KY-31’ stockpiled tall fescue (2015-2017). The three studies were conducted at the University of Kentucky Spindletop Research Farm in Lexington, KY in a randomized complete block design. In the bermudagrass-white clover study, all enhanced efficiency N sources maintained white clover populations similar to the unfertilized grass/clover control, but only ESN caused greater clover composition than standard urea. Total forage yields increased linearly with N rate in all years, but dry weather conditions in the second and third years resulted in lower total yield. Forage nutritive value followed general trends throughout each growing season, but ESN’s ability to maintain clover resulted in higher nutritive value. In the tall fescue-red clover, total forage yields curvilinearly increased with N rate in 2015 but did not vary in 2016. ESN and ESN+urea blend treatments retained clover composition similar to that of the unfertilized control. Stockpiled forage yield increased with higher N rates. Enhanced efficiency N fertilizers with the ability to control N release can enhance forage yield while maintaining clover in mixed species swards.

O presente estudo avaliou a composição de bandos mistos de aves em uma paisagem de Floresta Atlântica, verificando se há estratificação entre os bandos de acordo com o estrato vertical vegetacional utilizado pelas espécies, e como os aspectos da paisagem influenciam a composição dos bandos mistos. Onze remanescentes florestais localizados na Bacia do rio Itajaí, Santa Catarina, Brasil, foram caracterizados pelo grau de conectividade com outras áreas florestais, sendo divididos em três categorias: 1) maciço; 2) conectado; e 3) isolado. Foram obtidas informações referentes à intensidade da perda de hábitat no entorno imediato das áreas. Foram registradas 117 espécies de aves, em uma média de 8,68 espécies por bando. A Ordem Passeriformes correspondeu a 88,89% das espécies amostradas, em sua maioria representantes das famílias Tyrannidae, Thraupidae, Thamnophilidae e Furnariidae. Outras ordens registradas foram Cuculiformes, Apodiformes, Trogoniformes e Piciformes. Mais de 70% das espécies ocorreram ocasionalmente nos bandos mistos. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus e Dysithamnus mentalis obtiveram os maiores valores de FO. Basileuterus culicivorus foi a única espécie-núcleo dos bandos mistos. Os bandos não apresentaram divisão clara quanto ao estrato utilizado para forrageio. As áreas de estudo formaram dois grupos distintos quanto à composição de espécies participantes dos bandos mistos, porém, não foi verificada relação com possíveis efeitos da fragmentação florestal. Os grupos diferiram quanto à altitude, reforçando a sugestão de que os bandos mistos são reflexo do pool de espécies local. / This paper examined the composition of mixed-species bird flocks in an Atlantic Forest landscape, evaluating the vertical vegetative stratification of the mixed-species flocks, and how landscape aspects influence the mixed-species flocks composition. Eleven forest remnants were selected and characterized according to their degrees of connectivity with other fragments, and divided in three categories: 1) massive; 2) connected; and 3) isolated. It was obtained information about the intensity of loss of habitat around the remnants. It was registered 117 bird species, in an average of 8.68 species per flock. The Passeriformes accounted 88.89% of the records, the majority Tyrannidae, Thraupidae, Thamnophilidae, and Furnariidae. Other orders recorded were Cuculiformes, Apodiformes, Trogoniformes, and Piciformes. More than 70% of the species were registered as occasional mixed-flocks. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus, and Dysithamnus mentalis obtained the highest values in occurrence frequency (OF). Only Basileuterus culicivorus was considered nuclear-species to the mixed flocks. The mixed-species bird flocks did not have a clear division among foraging stratus. The remnants formed two distinct groups to the mixed flocks species composition, but it was not checked relationship with forest fragmentation effects. The groups presented altitudinal difference, suggesting that the mixed flocks are a reflection to the local species pool.

O presente estudo avaliou a composição de bandos mistos de aves em uma paisagem de Floresta Atlântica, verificando se há estratificação entre os bandos de acordo com o estrato vertical vegetacional utilizado pelas espécies, e como os aspectos da paisagem influenciam a composição dos bandos mistos. Onze remanescentes florestais localizados na Bacia do rio Itajaí, Santa Catarina, Brasil, foram caracterizados pelo grau de conectividade com outras áreas florestais, sendo divididos em três categorias: 1) maciço; 2) conectado; e 3) isolado. Foram obtidas informações referentes à intensidade da perda de hábitat no entorno imediato das áreas. Foram registradas 117 espécies de aves, em uma média de 8,68 espécies por bando. A Ordem Passeriformes correspondeu a 88,89% das espécies amostradas, em sua maioria representantes das famílias Tyrannidae, Thraupidae, Thamnophilidae e Furnariidae. Outras ordens registradas foram Cuculiformes, Apodiformes, Trogoniformes e Piciformes. Mais de 70% das espécies ocorreram ocasionalmente nos bandos mistos. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus e Dysithamnus mentalis obtiveram os maiores valores de FO. Basileuterus culicivorus foi a única espécie-núcleo dos bandos mistos. Os bandos não apresentaram divisão clara quanto ao estrato utilizado para forrageio. As áreas de estudo formaram dois grupos distintos quanto à composição de espécies participantes dos bandos mistos, porém, não foi verificada relação com possíveis efeitos da fragmentação florestal. Os grupos diferiram quanto à altitude, reforçando a sugestão de que os bandos mistos são reflexo do pool de espécies local. / This paper examined the composition of mixed-species bird flocks in an Atlantic Forest landscape, evaluating the vertical vegetative stratification of the mixed-species flocks, and how landscape aspects influence the mixed-species flocks composition. Eleven forest remnants were selected and characterized according to their degrees of connectivity with other fragments, and divided in three categories: 1) massive; 2) connected; and 3) isolated. It was obtained information about the intensity of loss of habitat around the remnants. It was registered 117 bird species, in an average of 8.68 species per flock. The Passeriformes accounted 88.89% of the records, the majority Tyrannidae, Thraupidae, Thamnophilidae, and Furnariidae. Other orders recorded were Cuculiformes, Apodiformes, Trogoniformes, and Piciformes. More than 70% of the species were registered as occasional mixed-flocks. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus, and Dysithamnus mentalis obtained the highest values in occurrence frequency (OF). Only Basileuterus culicivorus was considered nuclear-species to the mixed flocks. The mixed-species bird flocks did not have a clear division among foraging stratus. The remnants formed two distinct groups to the mixed flocks species composition, but it was not checked relationship with forest fragmentation effects. The groups presented altitudinal difference, suggesting that the mixed flocks are a reflection to the local species pool.

O presente estudo avaliou a composição de bandos mistos de aves em uma paisagem de Floresta Atlântica, verificando se há estratificação entre os bandos de acordo com o estrato vertical vegetacional utilizado pelas espécies, e como os aspectos da paisagem influenciam a composição dos bandos mistos. Onze remanescentes florestais localizados na Bacia do rio Itajaí, Santa Catarina, Brasil, foram caracterizados pelo grau de conectividade com outras áreas florestais, sendo divididos em três categorias: 1) maciço; 2) conectado; e 3) isolado. Foram obtidas informações referentes à intensidade da perda de hábitat no entorno imediato das áreas. Foram registradas 117 espécies de aves, em uma média de 8,68 espécies por bando. A Ordem Passeriformes correspondeu a 88,89% das espécies amostradas, em sua maioria representantes das famílias Tyrannidae, Thraupidae, Thamnophilidae e Furnariidae. Outras ordens registradas foram Cuculiformes, Apodiformes, Trogoniformes e Piciformes. Mais de 70% das espécies ocorreram ocasionalmente nos bandos mistos. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus e Dysithamnus mentalis obtiveram os maiores valores de FO. Basileuterus culicivorus foi a única espécie-núcleo dos bandos mistos. Os bandos não apresentaram divisão clara quanto ao estrato utilizado para forrageio. As áreas de estudo formaram dois grupos distintos quanto à composição de espécies participantes dos bandos mistos, porém, não foi verificada relação com possíveis efeitos da fragmentação florestal. Os grupos diferiram quanto à altitude, reforçando a sugestão de que os bandos mistos são reflexo do pool de espécies local. / This paper examined the composition of mixed-species bird flocks in an Atlantic Forest landscape, evaluating the vertical vegetative stratification of the mixed-species flocks, and how landscape aspects influence the mixed-species flocks composition. Eleven forest remnants were selected and characterized according to their degrees of connectivity with other fragments, and divided in three categories: 1) massive; 2) connected; and 3) isolated. It was obtained information about the intensity of loss of habitat around the remnants. It was registered 117 bird species, in an average of 8.68 species per flock. The Passeriformes accounted 88.89% of the records, the majority Tyrannidae, Thraupidae, Thamnophilidae, and Furnariidae. Other orders recorded were Cuculiformes, Apodiformes, Trogoniformes, and Piciformes. More than 70% of the species were registered as occasional mixed-flocks. Basileuterus culicivorus, Xiphorhynchus fuscus, Sittasomus griseicapillus, Habia rubica, Philydor atricapillus, and Dysithamnus mentalis obtained the highest values in occurrence frequency (OF). Only Basileuterus culicivorus was considered nuclear-species to the mixed flocks. The mixed-species bird flocks did not have a clear division among foraging stratus. The remnants formed two distinct groups to the mixed flocks species composition, but it was not checked relationship with forest fragmentation effects. The groups presented altitudinal difference, suggesting that the mixed flocks are a reflection to the local species pool.

Maximum size-density relationships (MSDR) are used to quantify differences across sites in the number of trees of a given size and species that can be supported per hectare. These relationships are important to managers who are trying to maximize basal area and wood volume. In my study, I examined MSDR across Alabama, Georgia, Louisiana, and Mississippi using US Forest Service, Forest Inventory and Analysis (FIA) data. I determined the impact of species-specific, specific gravity, functional traits, and environmental factors on MSDR using a quantile regression approach. Overall, I found that climatic factors had the greatest influence on MSDR, and that species shade and drought tolerance were more influential than specific gravity across the southeastern US.

Many landowners in the southeastern United States have invested in southern yellow pine plantations and managed them using traditional management schemes. However, non-industrial private forest landowners often have goals and challenges that make traditional management less desirable or possible. This thesis contains two projects that offer potential solutions for these landowners. The first project is a management option for landowners with small parcels that wish to manage pine plantations and proposes low-density management paired with crown lifting for the production of saw logs without commercial thinning entries. The second proposes a method for incorporating mixed-species management into already existing plantations. This increases biodiversity and could be more ecologically stable than single-species plantations. These options present opportunities for non-industrial private forest landowners who do not fit into the traditional management framework due to differences in management goals or economic constraints on management.

2001 skedde ett kraftigt utbrott av Gremeniella abietina (Lagerb.) M. Morelet i området Bergvik, Dalarna. Skogsägarna var tvungna att avverka kraftigt i deras tallbestånd som respons på detta. I de objekt som återplanterades planterades det nya artsammansättningar än vad som planterats tidigare, gran och blandbestånd av gran och tall. I denna studie jämfördes och inventerades medelhöjderna på de återplanterade objektens träd för att skatta arternas framgång i de olika ståndorterna. Gran verkade vara mest lämpat eftersom den växte signifikant bättre än vad tillväxtekvationerna hade förutspått. Det påträffades även en positiv blandskogseffekt på trädens medelhöjd i provytorna. Provytor med högre antal arter, hade gran och tall med högre medelhöjd än artfattigare provytor. Provytor med tre arter, björk gran och tall hade högre tillväxt än provytor med endast tall och gran. Graden av inblandning i hela objektet hade inte någon signifikant effekt på medelhöjden. Detta tyder på att den spatiella artsammansättningen har större betydelse för de positiva effekterna av blandskog än artsammansättningen inom ståndorten i sin helhet. Detta antas vara på grund av nisch-komplementering och facilitering, möjligtvis via mykorrhiza interaktion. En annan möjlig förklaring är den minskade intra-specifika konkurrensen när individer av samma art är planterade med större avstånd sinsemellan. Troligtvis beror det på en nischuppdelning som tillåter de olika trädarterna att utnyttja markens tillgångar effektivare. Detta indikerar att småskalig artsammansättning är av vikt för tillväxten hos gran och tall. / In 2001 there was a serious outbreak of Gremmeniella abietina (Lagerb.) M. Morelet in the municipality of Bergvik in Dalarna, Sweden. The forest owners were forced to effectively cut their Pine stands in an attempt to halt the onslaught of the fungus. In the areas where new trees were planted after the cuttings, different compositions of species were implemented. Instead of only Pine (Pinus sylvestris), most of the new plantations consisted of spruce (Picea abies) or mixtures of the two species. In this study the average height of these replanted trees was measured to assess the success that the different species had in the different replanted areas. Spruce seemed to be the species that was best adapted to the local area because it grew significantly higher then what had been predicted. A positive effect of mixed-stands was also observed. The sample areas with three species, pine, spruce and birch (Betula pendula) had a higher average height then the sample areas that contained less species. The degree of mixture in the plantation as a whole had no significant effect on tree height. This implies that the spatial composition of species is of bigger importance then the quota of different species in the whole plantation. This could be explained by niche-complementation and by facilitation, probably via interactions between mykorrhiza. Intra-specific competition is another possible explanation for the greater height in sample areas with more species.  Most likely because a decrease in intra-specific competition allows a division of niches and therefore permits the resources in the soil to be utilized more efficiently. The implication of this being that small-scale specie composition is of importance.