This thesi s is eoneerned with the deteetion of posttranslational modifieation of proteins. These proteins are identified using liquid ehromatography and eapillary eleetrophoresis, their eoupling and eoupling to mass speetrometry. The first part of the theoretieal ehapter is devoted to the strueture, classifieation and metabolits ehanges of proteins in the human body. Major attention is foeused on eollagen, its strueture and individual eollagen types. The seeond part eonsiders the problems of nonenzymatie glyeation and its influenee on the body and engage in produets of posttranslational modifieation. The third part summarizes the individual separation proeedures and analysis of posttranslationaly modified proteins. The seeond ehapter deseribes experimental parameters, including ehemieals, instrumentation, materials and methods. The preparation of individual samples is also deseribed. All results and findings are summarized and diseussed in the last ehapter. This ehapter is divided in two parts. The first part foeuses on problems of in vivo experiments. The seeond part foeuses on problems of in vitro experiments. Powered by TCPDF (www.tcpdf.org)

The topic of this diploma thesis is body modification culture phenomenon called 'suspension'. It is a voluntary act of suspending a human body from hooks or piercings that have been temporarily pierced through the skin. Suspension is an age-old technique practiced for example by North American Indian Mandan tribe. It has been brought to the modern era and body modification culture by modern primitives. The part of the thesis is a placement of suspension to the broader sociocultural and historical context, there will be also space for development of concept of body, embodiment and pain in the western word and ritual aspect of suspension. The source for this work is mainly accessible literature, internet pages dedicated to body modifications and participant observation completed with interviews with people who had been suspended.