Copies of author's previously published articles inserted. / Bibliography: leaves 175-194. / xiii, 195 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eikenella corrodens, a Gram-negative rod, is a normal inhabitant of the human oral cavity. It is one of the most commonly detected cultivable bacteria from sub- and supra-gingival plaque and is often isolated in elevated proportions from sites exhibiting destructive periodontal disease compared with healthy sites. The primary aim of this study was to investigate the metabolism of E. corrodons, with particular reference to energy generation, and to determine the effect of physical environmental parameters on its growth. / Thesis (Ph.D.)--University of Adelaide, Dept. of Dentistry, 2000

Addenda inserted in back. / Includes bibliographical references (leaves 139-160) / vii, 163 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Assesses the importance of amino acids 221 to 236 of bIGFBP-2 for IGF binding activity, by creating amino acid substitutions. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 2001?

Contains copies of author's previously published articles. / Includes bibliographic references. / 1 v. various foliations : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Paediatrics, 1992

The largest of the 80 families in the division Rhodophyta is the Rhodomelaceae. A great many genera and species are restricted to Australasia, which is the major centre of diversity and distribution. Perhaps as a consequence, the Rhodomelaceae has remained relatively unstudied compared with most other red algal families even at the morphological/anatomical level. Within the confines of a PhD program it would not be possible to study all of the 125 genera credited to the Rhodomelaceae. The focus of this thesis has, therefore, been narrowed down to selected tribes and genera, all of which have a strong Australasian component. Anatomical and molecular analyses have been undertaken on members of the tribes Amansieae, Pleurostichidieae, Streblocladieae and Sonderelleae and the genus Halydictyon. The results of these analyses have led to the description of two new genera, three new species, and one new tribe as well as the resurrection of two genera and the recombination of ten species. The taxonomic position and validity of the studies tribes and genera has also been established.

Copies of author's previously published articles inserted. / Bibliography: leaves 175-194. / xiii, 195 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eikenella corrodens, a Gram-negative rod, is a normal inhabitant of the human oral cavity. It is one of the most commonly detected cultivable bacteria from sub- and supra-gingival plaque and is often isolated in elevated proportions from sites exhibiting destructive periodontal disease compared with healthy sites. The primary aim of this study was to investigate the metabolism of E. corrodons, with particular reference to energy generation, and to determine the effect of physical environmental parameters on its growth. / Thesis (Ph.D.)--University of Adelaide, Dept. of Dentistry, 2000

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Made available in DSpace on 2015-04-09T12:28:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-06-18Bitstream added on 2015-04-09T12:48:06Z : No. of bitstreams: 1 000816635.pdf: 1408307 bytes, checksum: 5daacbf627e5bb9b1039a599a8e5bed4 (MD5) / O presente estudo teve como objetivo avaliar a ocorrência da infecção e realizar a caracterização molecular de Cryptosporidium spp. em caprinos jovens do município de Quixadá, Ceará, Brasil. Participaram do estudo um total de 400 animais, com idade entre três e 360 dias, com e sem padrão racial definido, nos quais 154 eram machos e 246 fêmeas, procedentes de 25 estabelecimentos rurais distribuídos em três circuitos. Amostras de fezes foram obtidas diretamente da ampola retal dos cabritos e cadastradas de acordo com o aspecto e a coloração. Uma quantidade de 200 mg de cada amostra foi distribuída em microtubos de 2 mL e congeladas “in natura” a -20 °C, até o momento das extrações de DNA genômico do parasito com auxílio de kit comercial. Todas as amostras foram submetidas a “Nested”-PCR para amplificação de fragmentos do gene da subunidade 18S RNA ribossômico. As positivas nesta amplificação foram testadas na “Nested”-PCR com os genes actina e HSP 70. As amostras que tiveram amplificação nesses três genes foram submetidas a “Nested”-PCR em triplicata e posterior sequenciamento nas direções “sense” e “anti-sense”. A ocorrência da infecção por Cryptosporidium spp. em cabritos de Quixadá foi de 7,50% (30/400) e 64,00% (16/25) das propriedades tinham cabritos positivos. O distrito Tapuiará foi o que apresentou maior número de animais positivos, e a frequência no período seco e chuvoso foi de 9,55% (19/199) e 5,47% (11/201), respectivamente (P = 0,1218). Das 30 amostras de fezes positivas, 50,00% (15/30) tinham aspecto normal e 70,00% (21/30) coloração normal, sugerindo que cabritos assintomáticos eliminam oocistos no ambiente. Não foi observada positividade para Cryptosporidium spp. em animais com 301 a 360 dias. Por meio do sequenciamento foi possível identificar que as espécies do coccídio detectadas nas fezes dos cabritos foram Cryptosporidium xiaoi ... / The present study aimed to evaluate the occurrence of infection and perform molecular characterization of Cryptosporidium spp. in goats kids from the town of Quixadá – state of Ceará – Brazil. A total of 400 animals, 154 males and 246 females, with aged between three and 360 days, with and without defined breed, from 25 farms distributed in three circuits, participated in this study. Stool samples were obtained directly from the goats rectum and registered in accordance to their appearance and coloring. An amount of 200 mg of each sample, were distributed in micro tubes of 2 mL and frozen “in nature” at -20 °C until the moment of extraction of the parasite genomic DNA using a commercial kit. All samples were submitted to Nested-PCR to amplify fragments of 18S subunit of ribosomal RNA. The positives samples in this amplification were tested in Nested-PCR with actin and HSP 70 genes. Samples that were amplified in these three genes were subjected to Nested-PCR triplicate and subjected to sequencing in sense and anti-sense directions. The occurrence of the infection by Cryptosporidium in goats kids of the town of Quixadá was 7.50% (30/400) and 64.00% (16/25) of the farms with positive goats. The Tapuiará district showed the highest number of positive animals and the frequency in dry and rainy period was 9.55% (19/199) and 5.47% (11/201), respectively (P = 0.1218). Considering 30 samples of positive stools, 50.00% (15/30) had normal appearance and 70.00% (21/30) normal coloring, suggesting that asymptomatic goats can eliminate oocysts in the environment. It was not observed positivity for Cryptosporidium spp. in animals aged between 301 and 360 days. Through sequencing, it was possible to identify that the species of coccidian found in the feces of the goats are Cryptosporidium xiaoi, Cryptosporidium ubiquitum and Cryptosporidium meleagridis. Different species of protozoa are present in rural farms ...

Cellular redox states mediate various physiological and developmental processes. Mechanisms involved in sensing cellular redox state and linking it to an appropriate physiological response remains poorly understood in plants. Arabidopsis bZIP68 was previously found to undergo reversible oxidation in its Cys320 in cells under oxidative stress. In this study, it was found that bZIP68 was localized in the nucleus in Arabidopsis seedlings under normal conditions. Upon treatment of oxidative stress, bZIP68 underwent nucleocytoplasmic shuttling and accumulated in the cytoplasm. This stress-dependent nucleocytoplasmic shuttling depends on the redox-sensitive Cys320 and its nuclear export signal. bZIP68 suppresses expression of stress response genes under normal conditions and its loss-of-function mutation of bZIP68 leads to elevated expression of genes involved in oxidative stress defense including genes encoding for antioxidant proteins and for enzymes involved in biosynthesis of small molecule antioxidants. The bzip68 mutant also showed enhanced responses to stress treatment such as the oxidative stress and cold stress. Our study suggests that bZIP68 directly or indirectly senses perturbation of cellular redox states and links the redox change to activation of oxidative stress defense genes through redox regulation of transcription.

Orientador: Katia Denise Saraiva Bresciani / Coorientador: Luiz da Silva Vieira / Coorientador: Marcelo Vasconcelos Meireles / Banca: Márcia Benedita de Oliveira Silva / Banca: Jancarlo Ferreira Gomes / Banca: Luis Antonio Mathias / Banca: Gilson Helio Toniollo / Resumo: O presente estudo teve como objetivo avaliar a ocorrência da infecção e realizar a caracterização molecular de Cryptosporidium spp. em caprinos jovens do município de Quixadá, Ceará, Brasil. Participaram do estudo um total de 400 animais, com idade entre três e 360 dias, com e sem padrão racial definido, nos quais 154 eram machos e 246 fêmeas, procedentes de 25 estabelecimentos rurais distribuídos em três circuitos. Amostras de fezes foram obtidas diretamente da ampola retal dos cabritos e cadastradas de acordo com o aspecto e a coloração. Uma quantidade de 200 mg de cada amostra foi distribuída em microtubos de 2 mL e congeladas "in natura" a -20 °C, até o momento das extrações de DNA genômico do parasito com auxílio de kit comercial. Todas as amostras foram submetidas a "Nested"-PCR para amplificação de fragmentos do gene da subunidade 18S RNA ribossômico. As positivas nesta amplificação foram testadas na "Nested"-PCR com os genes actina e HSP 70. As amostras que tiveram amplificação nesses três genes foram submetidas a "Nested"-PCR em triplicata e posterior sequenciamento nas direções "sense" e "anti-sense". A ocorrência da infecção por Cryptosporidium spp. em cabritos de Quixadá foi de 7,50% (30/400) e 64,00% (16/25) das propriedades tinham cabritos positivos. O distrito Tapuiará foi o que apresentou maior número de animais positivos, e a frequência no período seco e chuvoso foi de 9,55% (19/199) e 5,47% (11/201), respectivamente (P = 0,1218). Das 30 amostras de fezes positivas, 50,00% (15/30) tinham aspecto normal e 70,00% (21/30) coloração normal, sugerindo que cabritos assintomáticos eliminam oocistos no ambiente. Não foi observada positividade para Cryptosporidium spp. em animais com 301 a 360 dias. Por meio do sequenciamento foi possível identificar que as espécies do coccídio detectadas nas fezes dos cabritos foram Cryptosporidium xiaoi ... / Abstract: The present study aimed to evaluate the occurrence of infection and perform molecular characterization of Cryptosporidium spp. in goats kids from the town of Quixadá - state of Ceará - Brazil. A total of 400 animals, 154 males and 246 females, with aged between three and 360 days, with and without defined breed, from 25 farms distributed in three circuits, participated in this study. Stool samples were obtained directly from the goats rectum and registered in accordance to their appearance and coloring. An amount of 200 mg of each sample, were distributed in micro tubes of 2 mL and frozen "in nature" at -20 °C until the moment of extraction of the parasite genomic DNA using a commercial kit. All samples were submitted to Nested-PCR to amplify fragments of 18S subunit of ribosomal RNA. The positives samples in this amplification were tested in Nested-PCR with actin and HSP 70 genes. Samples that were amplified in these three genes were subjected to Nested-PCR triplicate and subjected to sequencing in sense and anti-sense directions. The occurrence of the infection by Cryptosporidium in goats kids of the town of Quixadá was 7.50% (30/400) and 64.00% (16/25) of the farms with positive goats. The Tapuiará district showed the highest number of positive animals and the frequency in dry and rainy period was 9.55% (19/199) and 5.47% (11/201), respectively (P = 0.1218). Considering 30 samples of positive stools, 50.00% (15/30) had normal appearance and 70.00% (21/30) normal coloring, suggesting that asymptomatic goats can eliminate oocysts in the environment. It was not observed positivity for Cryptosporidium spp. in animals aged between 301 and 360 days. Through sequencing, it was possible to identify that the species of coccidian found in the feces of the goats are Cryptosporidium xiaoi, Cryptosporidium ubiquitum and Cryptosporidium meleagridis. Different species of protozoa are present in rural farms ... / Doutor