No description available.

In the present work we evaluated the degree of sexual dimorphism in two cell groups of the medial preoptic-anterior hypothalamus (MPOA-AH) in monogamous and polygamous voles. Quantitative determinations were made of volume, cell number, and cell density for the anteroventral-periventriular nucleus (AVPV) and the sexually dimorphic nucleus of the preoptic area (SDN-POA). Polygamous montane voles (Microtus montanus) had a greater degree of sexual dimorphism in both cell groups than did monogamous prairie voles (M. ochrogaster). Most notable was the complete absence of the AVPV in male montane voles; male montane voles also had a significantly larger SDN-POA volume than did females. The only sexual dimorphism in prairie voles was a greater cell density in the female AVPV. In addition, prairie voles had larger relative brain size than did montane voles. Comparative behavioral studies have revealed a correlation between the degree of sexual dimorphism in external morphology and mating system, i.e., polygamous species display greater levels of dimorphism than do monogamous species. The present results indicate that the effects of sexual selection can also be seen in those brain regions, like the hypothalamus, that underlie social and reproductive behavior. Moreover, these results support the hypothesis that neuroanatomic dimorphisms in the MPOA-AH may be related to sex differences in behavior.

Montane meadows provide essential habitat for a variety of unique species and important ecosystem services in the western United States. Although important, meadows have experienced increased rates of conifer encroachment due to climate change, fire suppression and grazing. To combat meadow degradation from conifer encroachment, land managers have employed various restoration strategies one of which is conifer removal. Multiple studies have investigated the relationship between meadow hydrology and vegetation; however, few have assessed the effect of conifer removal on meadow groundwater. The goal of this study is to determine if the removal of conifers from an encroached meadow has an effect on depth to the groundwater table (WTD) and soil moisture content (SMC), and to investigate the accuracy and potential usefulness of evapotranspiration (ET) calculation methodologies for montane meadows. This goal will be accomplished by the subsequent objectives: 1) perform an analysis of WTD and SMC in an encroached meadow preceding and following conifer removal and upland thinning; 2) calculate and compare daily ET estimates in a previously restored meadow using diurnal groundwater table fluctuation, diurnal groundwater fluctuation modelling, and SMC. Miranda Cabin Meadow (MC) is located within the Upper American River Watershed, southeast of French Meadows Reservoir, at an elevation of 6,200 feet. MC received conifer removal, upland thinning and road decommissioning in the fall of 2018 as part of the American River Conservancy’s American River Headwaters Restoration Project. This study found the average WTD in MC during the growing season decreased from 4.91 feet prior to restoration, to 3.39 feet after restoration. In addition, the number of days the WTD was within 0.98 feet and 3 feet increased from 12 days and 34 days, to 31 and 49 days. Analysis of SMC in MC was limited due to gaps in data, however this study found that after restoration the average weekly SMC decreased at a slower rate than prior to restoration, possibly indicating decreased hydrologic output from ET. Based upon WTD during the growing season and the limited SMC data it appears that removal of conifers and upland thinning at MM promotes SMC and WTD conditions conducive to meadow vegetation communities. Marian Meadow (MM), located in Plumas County, CA at an elevation of 4,900 feet, received conifer removal as part of a timber harvest plan carried out by Collins Pine Company in July 2015. The soil moisture sensors used in this study were installed in MM in September 2013 for previous graduate thesis research. Groundwater table data was collected using 10-foot wells installed in July of 2018. Daily ET was calculated during August 2018 using three methodologies, and during September 2018 using two methodologies. Daily ET estimates calculated using diurnal groundwater table fluctuation and the White method averaged 11.8 mm per day in August and 9.1 mm in September. Using diurnal groundwater table fluctuation modelling this study calculated an average daily ET of 4.2 mm in August and 3 mm in September. Daily ET estimates based on SMC were calculated for August 2018 using two methods which produced estimates of 0.9 mm and 1.2 mm per day. All three methods for calculating ET produced some daily estimates that compare well to previous research of Et in Sierra Nevada meadows, however the White method generally overestimated daily Et while SMC methods underestimated ET. Groundwater table fluctuation modelling produced the best estimates of daily ET for both August and September. ET results in this study support previous research on the applicability of the White method; and they also suggest that the applicability of groundwater fluctuation modelling to estimate meadow daily ET in Sierra Nevada montane meadows be investigated further.

An increasing amount of evidence suggests that as temperatures increase, montane animals are moving upward in elevation (IPCC 2007, Parmesan and Yohe 2003). As suitable habitats rise in elevation and then disappear altogether, these animals could be pushed to extinction. The American pika, Ochotona princeps, is a montane mammal that lives in western North America, usually at elevations above 1500 m (Smith and Weston 1990). Recent evidence suggests that pika population numbers are dropping in response to rising temperatures (Beever et al. 2010). The pika is a small herbivorous lagomorph, a relative of hares and rabbits. Its habitat is tightly restricted to talus slopes (rockfields) and the surrounding vegetation (Grayson 2005). Pikas have a high tolerance for cold temperatures, and do not hibernate during the long montane winter. However, they have very little tolerance for even mildly warm temperatures, and have been found to die when confined above ground at 25.5˚ C (Smith 1974b). To better understand pika persistence, we resurveyed 17 historic pika sites in the Lassen Peak region of northern California in August and September, 2009. Six of the historic sites were abandoned, as well as an additional 11 of 17 new sites surveyed. At each site we collected habitat information, and analyzed the data for factors that were correlated with site occupancy. We also installed 38 iButton thermal dataloggers in abandoned and occupied pika use sites, to determine if temperature affects occupancy. The dataloggers remained in pika sites for 14 months and recorded temperature every 1.5 hours. Abandoned pika sites had higher average temperatures and more days below 0˚ C. They also had greater shrub cover, less forb and graminoid cover, and a greater percentage of litter substrate. These findings suggest that the current warming trend may be having a negative impact on pikas in the Lassen Peak Region. As temperatures rise, pikas may be declining due to unsuitable temperatures and altered vegetative communities. In addition to the Lassen surveys, I investigated pika behavior in different temperature regimes in the Sierra Nevada. If pikas are able to adapt to climate change, it is possible that populations of pikas in different temperature regimes may exhibit behavioral plasticity, or have evolved genetic differences, such that these populations have different daily activity schedules. To determine if there is a difference in pika behavior at different elevations I observed pikas in one low and one high elevation site within the Bishop Creek drainage system in the Sierra Nevada. I conducted behavioral observations of pikas in four time blocks throughout the day in August and September, 2010. I recorded specific behaviors, such as foraging and haying (vegetation collecting), and compared these activities between low and high elevation pikas at different times of day. In August, pikas in the low elevation site exhibited a different activity profile than those in the high elevation site. Low elevation pikas were significantly more crepuscular (most active at dawn and dusk) during this month. I also observed more foraging behavior in the high elevation than the low elevation site, in both August and September. Reduced activity at higher temperatures may have negative impacts on pikas as temperatures increase. Low elevation pikas may be stressed due to reduced time spent foraging and haypile (overwinter vegetation cache) gathering. However, if pikas were able to switch their activity schedules to a more nocturnal schedule, they could escape higher daytime temperatures. To detect the possibility of nocturnal behavior in low elevation pikas, I set up four infra-red remote cameras in the low elevation site. I had variable success in capturing pika behavior with the cameras, and detected no evidence of nocturnal behavior. More research on the possibility of nocturnal behavior in pikas would be worthwhile, in part to determine what chance, if any, pikas have of adapting to rising global temperatures.

Montane forests provide habitat for unique assemblages of flora and fauna that contribute significantly to a region’s biodiversity. Previous work indicates that montane forest ecosystems are exceedingly vulnerable to a host of anthropogenic stressors including climate change, atmospheric deposition, and recreation, to name a few. Montane forests and other high elevation ecosystems are considered to be among the first and most severely impacted by climate change. It is therefore, imperative to evaluate anthropogenic impacts on montane ecosystems and maintain reliable monitoring methods that are capable of tracking potential shifts in the distribution of species dependent on these systems. I surveyed birds at various distances from hiking trails in the White Mountain National Forest from 2006 - 2009 to determine whether existing monitoring programs, all of which are based on trail-centered surveys, are accurately reflecting bird abundance, abundance stability and recruitment. Contrary to previous studies, I found that recreational trails generally did not alter estimates of abundance, recruitment, abundance stability, and detection probability for five species of birds considered to be indicators of montane forest ecosystem integrity in northeastern North America. Therefore, trail-based monitoring programs for montane birds appear to accurately reflect dynamics of bird communities undisturbed by hiking trails. These conclusions were supported by my finding that the daily nest survival of a montane spruce-fir indicator species, blackpoll warbler (Steophaga striata), did not vary as a function of distance from trail. I then used data from the White Mountain National Forest’s montane bird monitoring program from 1994 through 2009 to assess potential shifts in the elevational distribution of montane birds in conjunction with documented habitat shifts in the region. My results provide evidence that low elevation forest birds have expanded their upper elevational boundary while high elevation birds have expanded their lower elevation boundary. These results highlight the complicated relationship between habitat, climate, and other anthropogenic stressors such as atmospheric deposition and that even in the face of climate change other stressors may be playing a significant role in shifts of species distributions. Understanding how climate affects the reproductive ecology of montane organisms is an important step toward unraveling the potential mechanisms by which climate change will alter the distribution of these species. I used blackpoll warbler breeding data from the Green Mountains, VT from 1994 to 2003 to determine if temporal variation in climate influenced blackpoll nesting initiation and found that years with warm Mays and typical precipitation lead to earlier nest initiation. I also examined the effect of spatial variation in climate on blackpoll reproductive ecology and demography. I found a gradient in habitat quality associated with the spatial variation in climate along an elevation gradient. Blackpolls were less abundant, younger, had lower pairing success, lower daily nest survival, higher nest predator occupancy, and lower fecundity at lower elevations. The climatic conditions at these lower elevations represent the climatic conditions predicted to encompass increasingly larger portions of montane areas. Collectively, these findings contribute to filling in a dearth of knowledge regarding management and an understanding of how species dependent on montane ecosystems are responding to climate change.

No description available.

The economic evaluation of costs for remediation of montane spruce forest attacked by bark beetle in the different stage of decline (plots with actually living mature trees, plots with dead tree stand, wood is remaining in the ecosystem, plots with damaged stands, which were clear-cut, ten model plots) was made in the National Nature Park Šumava (Modrava model area) using resource equivalency method. Microclimatic characteristics (temperature and humidity development) measured ba dataloggers and communities of epigeic beetles (pitfall traps) were used as environmental metrics. Results indicate that the natural remediation of declined forest is economically most profitable. The microclimatic characteristics of plots with dead tree stand are most similar to the active forest. The species diversity, activity of beetles and frequency of relic species and species indicating virginal forest is higher in plots with dead tree stands. Based on these data we can resulted that the natural remediation of montane spruce forest is the most acceptable way both from biological and economical view (regeneration of ecosystem services of montane spruce forest).

How and why species’ ranges shift has long been a focus of ecology but is now becoming increasingly important given the current rate of climatic and environmental change. In response to global warming, species will need to migrate northward or upward to stay within their climatic tolerances. The ability of species to migrate will determine their fate and affect the community compositions of the future. However, to more accurately predict the future extent of species, we must identify and understand their responses to past and current climatic and environmental changes. The first place change is expected to occur is within ecotones where the ranges of many species converge and individuals exist at the limits of their environmental tolerances. In montane regions, these boundaries are compressed, creating a situation in which even relatively small changes in conditions can lead to shifts in the elevational ranges of species. In this dissertation, I examine the responses of forests in the Green Mountains of Vermont to recent climatic and environmental change in an attempt to understand how future climate change will affect their location and composition. I focus on the Boreal-Deciduous Ecotone (BDE), where the high elevation spruce-fir forests converge with the lower elevation northern hardwoods. In addition to investigating adult trees within the BDE, I also examine the responses of understory herbs and tree seedlings to changes in environmental and climatic factors. Factors considered in these investigations include temperature, soil environment, light environment, invasive species, competition, disturbance and many others. I will examine the complex range of responses in forest species that results from prolonged exposure to these forces alone and in combination. I have attempted to identify the responses of forest species to environmental changes by resurveying historic vegetation plots (Chapter 2), experimentally manipulating the growing environment of tree seedlings (Chapter 3) and performing dendrochronological analyses on tree rings (Chapter 4). Through my resurvey of historic vegetation plots, I determined the degree to which understory species have shifted as individuals or as groups. I also identified a set of novel understory communities that have developed since the 1960's in response to recent climate change, acid deposition and invasive species (Chapter 2). By transplanting and artificially warming tree seedlings, I identified factors responsible for limiting the growth and survival of northern hardwood species above the BDE. Temperature was the primary factor limiting sugar maple (Acer saccharum) at high elevations, while yellow birch (Betula alleghaniensis) was limited almost exclusively by light (Chapter 3). Dendrochronological studies of sugar maples indicated that prolonged exposure to acidified soils has only recently caused growth declines and has altered their relationship to climate (Chapter 4). Together, these studies have produced a cohesive picture of how northeastern montane forests have responded to recent climate change and other anthropogenic impacts. These findings can be used to help predict future species' ranges and identify species that may not be capable of migrating fast enough on their own to keep pace with changes in climatic conditions.

Entre outubro de 2009 e dezembro de 2012, foi realizado estudo no Laboratório de Hidrologia Florestal Walter Emmerich (L.H.F.W.E.), para avaliar o potencial hidrológico dos nevoeiros e a precipitação oculta em ambiente de Floresta Ombrófila Densa Montana, na Serra do Mar, em Cunha, a leste do Estado de São Paulo. Os objetivos específicos da pesquisa foram: 1) avaliar o desempenho de coletores de água de nevoeiro; 2) verificar o potencial de coleta de água de nevoeiro no laboratório; 3) analisar o potencial de coleta de água de nevoeiro em três setores da microbacia experimental D; 4) averiguar a distribuição espacial e temporal de ocorrência dos nevoeiros na microbacia D e 5) estimar a contribuição da precipitação oculta na floresta da microbacia D. Para alcançar os três primeiros objetivos foram usados coletores passivos tipo harpa, nas formas cilíndrica e plana. Para estudar a distribuição de nevoeiros, foram feitas observações diárias de três referências visuais situadas a distâncias conhecidas no interior da microbacia D. O método da medição da precipitação efetiva foi empregado para estimar a precipitação oculta na floresta. Sobre a distribuição espacial e temporal da ocorrência de nevoeiros na microbacia D, os resultados mostraram que os episódios de nevoeiro foram mais comuns à tarde, como decorrência da brisa marítima proveniente do Oceano Atlântico. Para 61,3% do tempo, em média, foi observada a presença de nevoeiro em algum setor da microbacia, com a ocorrência dos nevoeiros diminuindo de montante para jusante da área. No que concerne ao desempenho dos equipamentos, a média de coleta diferiu de coletor para coletor, exceto entre o coletor cilíndrico descoberto e o coletor plano, que interceptaram mais água que os coletores cilíndricos cobertos. O coletor plano foi o único a interceptar água em todos os episódios amostrados. Sobre o potencial de coleta de água de nevoeiros no laboratório, concluiu-se que é reduzido e resulta da combinação de três fatores: a grande ocorrência de nevoeiros de radiação, a curta duração dos ix episódios dos nevoeiros orográficos e a baixa intensidade dos ventos na região. No que concerne ao potencial de coleta de água de nevoeiro em três setores da microbacia D, árvores localizadas próximas aos coletores funcionaram como obstáculo à livre circulação dos nevoeiros orográficos, induzindo os coletores a interceptar exíguos volumes de água. Considerando apenas os eventos de nevoeiro sem chuva, a precipitação oculta foi de 2,3 mm, correspondendo a 0,12% da precipitação pluviométrica anual. Para os eventos de chuva com a presença de nevoeiro, a precipitação oculta gerou um total de 5,1 mm de água adicional ao piso da floresta, correspondendo a 0,27% da precipitação pluviométrica. Concluiu-se que a precipitação oculta na floresta não é um processo importante para a entrada de água na microbacia D. / Between October 2009 and December 2012 a study was conducted at the Walter Emmerich Hydrologic Laboratory (LHFWE) to assess the hydrological potential of fog and occult precipitation in the Montane Dense Ombrophilous Forest environment located in the Parque Estadual da Serra do Mar - Núcleo Cunha, east of the State of São Paulo, Brazil. The specific multiple objectives of the research were: 1) evaluate the performance of passive fog collectors, 2) evaluate the potential for collecting fog water in the laboratory, 3) evaluate the potential of collecting fog water in three sectors of experimental catchment \"D\", 4) determine the spatial and temporal distributions of fog occurrence in catchment \"D\", 5) estimate the contribution of occult precipitation in the rainforest in catchment \"D\". To achieve the first three objectives, cylindrical and flat harp collectors were used. To study the spatial and temporal distributions of fog occurrence, we made daily observations of three visual references located at known distances within the catchment. Net precipitation was measured and used to estimate the occult precipitation in the rainforest. Regarding the spatial and temporal distributions of fog occurrence, the results showed that episodes of fog were more common in the afternoon as a result of the sea breeze from the Atlantic Ocean. For 61.3% of the time on average, we observed the presence of fog in some sector of the catchment, with its occurrence decreasing in areas located from upstream to downstream. Concerning the comparison of the performance of the collectors, the average volume of water collected differed from collector to collector, except between cylindrical collector uncovered and flat collector, which captured more water than the cylindrical collectors covered to prevent rainwater input. The flat collector was the only gauge to intercept water in all episodes. The high incidence of fog radiation, the short duration of orographic fog, and the low intensity of winds combined to reduce the potential for harvesting fog water in the laboratory. Regarding the potential for collecting fog water in three sectors of the catchment \"D\", xi trees located next to collectors operated as an obstacle to the free movement of orographic fog inducing gauges to intercept exiguous volumes of water. Considering only fog, but no rain, occult precipitation was 2.3 mm, corresponding to 0.12% of annual rainfall. For rainfall events with the presence of fog, occult precipitation generated a total of 5.1 mm of additional water to the floor of the forest, equivalent to 0.27% of the rainfall. It was therefore concluded that occult precipitation in rainforest did not significantly contribute to water entering catchment \"D\".

Tropical soils are a globally important store of terrestrial carbon (C) and source of atmospheric carbon dioxide (CO2), regulated by the activity of soil microorganisms, through the mineralisation of plant residues and soil organic matter (SOM). Climatic warming will influence microbial activity, and this may accelerate the rate of C release from soils as CO2, contributing to alterations in current atmospheric composition, and generating feedbacks to climate change. Yet the magnitude of C loss from tropical soils remains uncertain, partly because we do not fully understand how non-climatic factors – including the chemistry of plant inputs, the availability of soil nutrients and the composition of the decomposer community – will interact to determine the response to changes in temperature. This thesis examines how these factors together regulate the rate of C cycling in contrasting soils across a 3400 m tropical elevation gradient in the Peruvian Andes, spanning a 20 ºC range (6.5 – 26.4 ºC) in mean annual temperature. Large-scale field-based manipulation experiments, translocating leaves and soil-cores across the elevation gradient (to impose an in-situ experimental warming treatment), were combined with controlled laboratory studies to examine the microbial-scale mechanisms which underlie the processes of decomposition and soil respiration observed in-situ. Results show that, across the gradient, rates of leaf-decomposition were determined principally by temperature and foliar chemical traits, while soil fertility had no significant influence. The effect of temperature was, however, stronger across higher-elevation sites, suggesting a greater vulnerability of the C-rich soils in montane systems to increased C loss under climatic warming. In lowland forests, the presence of invertebrate macrofauna also accelerated rates of decomposition, but leaf chemistry explained the greatest proportion of the observed variance, with a strong role for leaf chemical traits also identified under controlled conditions. Despite marked differences in microbial abundance and community composition among soils, these metrics were not associated with observed rates of decomposition. These results suggest that climate-related changes to plant species distributions (with associated changes to the chemistry of leaf-inputs), and upslope extension of macrofaunal ranges, could strongly influence future rates of leaf decomposition, independently of the direct response to warming. From the soil translocation study, root-soil interactions stimulated substantial net C loss from montane soils following translocation downslope (experimental warming treatment), indicating that warming-related changes to root productivity, exudation and/or species-composition could represent an important mode of future C loss from these soils. To examine more closely how inputs of plant-derived C influence the turnover of pre-existing SOM, and whether soil nutrient availability modulates the response, soils were amended with simple and complex 13C-labelled substrates in combination with inorganic nutrient treatments. Isotopic partitioning was used to determine the degree to which C and nutrient inputs accelerated (positive priming) or retarded (negative priming) the decomposition of SOM. Amendment of upper montane forest and montane grassland soils with nitrogen (N; alone and in combination with C) substantially retarded the decomposition of SOM, suggesting that microbial demand for N strongly regulates the turnover of organic matter in these soils. In contrast, amendment of lower montane and lowland forest soils with C stimulated positive priming of SOM, which was strongest in response to the simple C substrate and was not influenced by nutrient treatments, suggesting that microorganisms in these soils are primarily constrained by availability of labile C. Functional differences among microbial groups were also evident, with gram-negative bacteria and fungi using more labile sources of C while gram-positive bacteria used more complex C. Together, results from these studies considerably advance our understanding of soil C dynamics across lowland and montane systems, painting a rich picture of interacting processes which will determine the future soil C balance in tropical ecosystems. They show that the influence of temperature on the rate of soil C cycling is strongly affected by the nature and composition of plant-derived and atmospheric inputs, the principal additional constraints varying with elevation, leading to both opposing and reinforcing effects on rates of decomposition. The greater observed temperature sensitivity of decomposition at higher elevations is coupled with high microbial demand for N which regulates the turnover of SOM, whereas at lower elevations leaf decomposition is accelerated by active macrofaunal breakdown, while microbial decomposition of SOM is constrained by the availability of labile C. Under a global change scenario of increased temperature and N deposition, results therefore suggest that: (i) modified chemistry of plant inputs will influence rates of decomposition, independently of climate; (ii) increased availability of labile C will lead to more rapid decomposition of SOM at lower elevations; (iii) greater root productivity (associated with warming and plant-community shifts) will stimulate soil C loss across montane regions; but (iv) at higher elevations, a possible countervailing effect may be imposed on rapid warming-accelerated decomposition if increased N availability reduces microbial mineralisation of SOM. The net effect on the ecosystem C budget will depend on the balance of C gain from primary productivity and C loss from soils. Overall, however, the results presented here suggest that the large soil C stores in higher-elevation montane regions are particularly vulnerable to substantial reductions under exposure to short- and medium-term climatic warming.