This thesis is based on study of archive material and it focuses on the Grey Sisters Congregation of the Third Order of St.Francis, its history and places of activity since its foundation in 1856 until today. The introductory chapters describe the context of the period, which triggered formation of this as well as many other women's congregations in the second half of the 19th century, and congregations as such. The following chapters are specifically about the Grey Sisters. At first it chronologically presents history of the Congregation. Next chapter is about the former motherhouse in Bartolomejska Street and the current motherhouse in Lomnice u Vodnan. Further on the thesis describes the history and work of the sisters in filial houses, which they let built in the 1930's, and in the nursing school which they founded in Hradec Kralove. In the last chapter the life of the congregation itself is captured. The appendix contains pictures, graphs and a map describing the places of activity of the sisters.