Thesis (Ph. D.)--University of North Texas, Dec., 2009. / Title from title page display. Includes bibliographical references.

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Made available in DSpace on 2014-06-11T19:27:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-24Bitstream added on 2014-06-13T20:36:05Z : No. of bitstreams: 1 gomes_ra_me_jabo.pdf: 463923 bytes, checksum: 093c31f69538b0fc07e18d34599830eb (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Objetivou-se com este estudo avaliar aspectos produtivos e nutricionais dos híbridos de amoreira FM Shima-Miura (SM) e IZ 56/4 (IZ), colhidas aos 30, 60, 90 e 120 dias de crescimento. Ao atingirem a idade de crescimento pré-estabelecida, as plantas foram colhidas, sendo folhas e caules pesados e amostrados separadamente. Foram realizadas análises de fracionamento, seguindo o esquema proposto pelo CNCPS, onde carboidratos foram separados em fração A, B1, B2 e C e os compostos nitrogenados nas frações A, B1+B2, B3 e C. Para estudo da cinética de degradação ruminal utilizou-se a técnica de produção de gases in vitro, sendo os dados obtidos ajustados ao modelo logaritmo bicompartimental. O híbrido SM apresentou produção de matéria seca (MS) e proteína bruta (PB) de folhas e caules superiores à IZ (P<0.05). Nas folhas, o híbrido IZ apresentou maiores proporções de componentes relacionados à parede celular, comparando-se com o SM (P<0.05). Não houve, no entanto, diferença entre híbridos na digestibilidade in vitro da matéria orgânica (DivMO) e nos parâmetros de produção de gases (P>0,05). Com o avanço da idade de crescimento, as folhas apresentaram aumento das frações B2 (de 108 a 146 g/kg de MS) e C (de 90 a 108 g/kg de MS) dos carboidratos (P<0,05), e redução nos de PB (de 224 a 191 g/kg de MS) para as idades de crescimento entre 30 e 120 dias. Contudo não houve alteração nos teores das frações A e B1 dos carboidratos, B1+B2, B3 e C dos compostos nitrogenados, na DivMO nem na produção de gases (P>0,05). Pelo desempenho produtivo superior, recomenda-se o híbrido SM para uso em sistemas de produção de ruminantes / The objective of this study was to evaluate the production and the nutritional aspects of mulberry hybrids FM Shima-Miura (SM) and IZ 56/4 (IZ), harvested at 30, 60, 90 and 120 days of regrowth. The plants were harvested, and leaves and stems were weighed separately. Carbohydrates and protein analyses followed the scheme proposed by CNCPS. Carbohydrates were separated into fractions A, B1, B2 and C and the nitrogen compounds, in the fractions A, B1+B2, B3 and C. To study the kinetics of ruminal degradation, we used the technique of in vitro gas production, and the data fit to the model log bicompartmental. The dry matter (DM) and crude protein (CP) contents of leaves and stems from SM hybrid were greater (P <0.05) than from IZ hybrid. The IZ hybrid had greater proportions of leaf components related to the cell wall compared to SM (P <0.05). However, there were no differences between hybrids in the in vitro digestibility of organic matter (ivOMD) and in the parameters of gas production (P> 0.05). With advancing age of regrowth, the leaves showed an increase of B2 (108- 146 g /kg of DM) and C (90 to 108 g / kg DM) carbohydrate fractions (P <0.05), and decrease in CP (224-191 g / kg DM) harvested between 30 to 120 days. However, there were no changes in the contents of carbohydrate fractions A and B1, nitrogen fractions B1+B2, B3 and C, ivOMD and parameters of gas production (P> 0.05). Due to its higher production, SM hybrid is recommended to use in ruminant production systems

Orientador: Kleber Tomás de Resende / Coorientador: Márcia Helena Machado da Rocha Fernandes / Banca: Ricardo Andrade Reis / Banca: Manoel Eduardo Rozalino Santos / Resumo: Objetivou-se com este estudo avaliar aspectos produtivos e nutricionais dos híbridos de amoreira FM Shima-Miura (SM) e IZ 56/4 (IZ), colhidas aos 30, 60, 90 e 120 dias de crescimento. Ao atingirem a idade de crescimento pré-estabelecida, as plantas foram colhidas, sendo folhas e caules pesados e amostrados separadamente. Foram realizadas análises de fracionamento, seguindo o esquema proposto pelo CNCPS, onde carboidratos foram separados em fração A, B1, B2 e C e os compostos nitrogenados nas frações A, B1+B2, B3 e C. Para estudo da cinética de degradação ruminal utilizou-se a técnica de produção de gases in vitro, sendo os dados obtidos ajustados ao modelo logaritmo bicompartimental. O híbrido SM apresentou produção de matéria seca (MS) e proteína bruta (PB) de folhas e caules superiores à IZ (P<0.05). Nas folhas, o híbrido IZ apresentou maiores proporções de componentes relacionados à parede celular, comparando-se com o SM (P<0.05). Não houve, no entanto, diferença entre híbridos na digestibilidade in vitro da matéria orgânica (DivMO) e nos parâmetros de produção de gases (P>0,05). Com o avanço da idade de crescimento, as folhas apresentaram aumento das frações B2 (de 108 a 146 g/kg de MS) e C (de 90 a 108 g/kg de MS) dos carboidratos (P<0,05), e redução nos de PB (de 224 a 191 g/kg de MS) para as idades de crescimento entre 30 e 120 dias. Contudo não houve alteração nos teores das frações A e B1 dos carboidratos, B1+B2, B3 e C dos compostos nitrogenados, na DivMO nem na produção de gases (P>0,05). Pelo desempenho produtivo superior, recomenda-se o híbrido SM para uso em sistemas de produção de ruminantes / Abstract: The objective of this study was to evaluate the production and the nutritional aspects of mulberry hybrids FM Shima-Miura (SM) and IZ 56/4 (IZ), harvested at 30, 60, 90 and 120 days of regrowth. The plants were harvested, and leaves and stems were weighed separately. Carbohydrates and protein analyses followed the scheme proposed by CNCPS. Carbohydrates were separated into fractions A, B1, B2 and C and the nitrogen compounds, in the fractions A, B1+B2, B3 and C. To study the kinetics of ruminal degradation, we used the technique of in vitro gas production, and the data fit to the model log bicompartmental. The dry matter (DM) and crude protein (CP) contents of leaves and stems from SM hybrid were greater (P <0.05) than from IZ hybrid. The IZ hybrid had greater proportions of leaf components related to the cell wall compared to SM (P <0.05). However, there were no differences between hybrids in the in vitro digestibility of organic matter (ivOMD) and in the parameters of gas production (P> 0.05). With advancing age of regrowth, the leaves showed an increase of B2 (108- 146 g /kg of DM) and C (90 to 108 g / kg DM) carbohydrate fractions (P <0.05), and decrease in CP (224-191 g / kg DM) harvested between 30 to 120 days. However, there were no changes in the contents of carbohydrate fractions A and B1, nitrogen fractions B1+B2, B3 and C, ivOMD and parameters of gas production (P> 0.05). Due to its higher production, SM hybrid is recommended to use in ruminant production systems / Mestre

Plant estrogens have long been associated with health benefits. The potential of tissue culture techniques for the production of several secondary metabolites has been known for many years. Tissue cultures stimulate the production or induce the biosynthesis of novel compounds not found in the mature plant. Tissue culture of Morus alba, family Moraceae, is known to contain phytoestrogens, was established on plant-hormone supplemented Murashige and Skoog (MS) medium. Petiole and the stem tissue from mature trees were the best explants for initiation and proliferation of calli. The best callus proliferation was obtained on MS medium containing 1-napthalene acetic acid (1mg/ml) and benzylaminopurine (0.5mg/ml) for M. alba. Comparison of phytoestrogens of Moraceae species from in vivo and in vitro tissue isolation were carried out. The estrogenic activities of callus extracts were assayed in an estrogen-responsive yeast system expressing the human estrogen receptor alpha. Male callus extracts had higher estrogenic activity than male and female extracts from in vivo and in vitro tissues. Isolation and characterization of phytoestrogens from above tissues were carried out using solid phase extraction, high perfomance liquid chromatography and mass spectrometry techniques. Biochanin A, an isoflavonoid, was isolated as one of the compounds in male callus extracts. Biochanin A has been known to have an antiestrogenic acitivity in mammals. Isoflavonoid compounds have been characterized as strong protein tyrosine kinase inhibitors in variety of animal cells. Isoflavones are structurally similar to estradiol, and display agonistic and antagonistic interactions with the estrogen receptor. Isoflavones possess therapeutic and preventive properties such as being used for postmenopausal osteoporosis, breast cancer, and inhibition of tumors.

Thesis (D. Min.)--New Orleans Baptist Theological Seminary, 2004. / Abstract and vita. Includes bibliographical references (leaves 115-123, 54-59). Includes final project proposal.

Thesis (D. Min.)--New Orleans Baptist Theological Seminary, 2004. / Includes abstract and vita. "November 2004." Includes bibliographical references (leaves 115-123).

Made available in DSpace on 2014-06-11T19:30:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-04-20Bitstream added on 2014-06-13T19:40:40Z : No. of bitstreams: 1 silva_rah_dr_arafcf_parcial.pdf: 156934 bytes, checksum: 0d16d4cb224d3289c64c5a248aa81946 (MD5) Bitstreams deleted on 2015-01-16T10:37:55Z: silva_rah_dr_arafcf_parcial.pdf,Bitstream added on 2015-01-16T10:38:38Z : No. of bitstreams: 1 000689668.pdf: 5100417 bytes, checksum: 67ceb9ad77d0b0ee40dda842049ac5ff (MD5) / Universidade Estadual Paulista (UNESP) / A espécie Morus nigra L. (Moraceae), amoreira negra, é originária do Sudoeste Asiático, sendo frequente nas regiões tropicais de todo o mundo. Do ponto de vista etnobotânico, a M. nigra possui popularidade por apresentar, não somente valores alimentares nutricionais, mas por conter vários compostos com ação terapêutica como adstringente, antiinflamatória, antioxidante e antitérmica, depurativa, analgésica, antisséptica, hipoglicemiante e usada no controle do climatério, não somente nos frutos, mas também em outras partes da planta como folhas e cascas da árvore. Os vegetais, presentes em grande quantidade na dieta humana, produzem um vasto número de substâncias naturais, chamados de metabólitos secundários como os compostos fenólicos, entre eles os flavonoides e polifenóis, que apresetam potencial antioxidante ou seja, são capazes de inibir ou atrasar os processos oxidativos, gerados por espécies oxidativas radicalares, ou não, retardando ou previnindo a oxidação de lipídios ou outras biomoléculas, evitando assim o surgimento de patologias relacionadas ao estresse oxidativo. O objetivo do trabalho foi avaliar o potencial antioxidante, antimicrobiano e antihemolítico do extrato bruto etanólico, obtidos por maceração de folhas de M. nigra e suas frações, produtos da partição liquido-líquido com hexano, acetato de etila e n-butanol. Para a avaliação da atividade antioxidante, foram utilizadas técnicas de supressão de espécies radicalares como DPPH, ABTS, anion superóxido e óxido nítrico, além de espécies oxidante não radicalar, como o HOCL. Também foi observado o efeito oxidorredutor das frações sobre a atividade catalítica da enzima horseradish peroxidase (HRP). A atividade antihemolítica foi avaliada... / The species Morus nigra L. (Moraceae), mulberry black, is indigenous to Southeast Asia and is common in tropical regions around the world. From the viewpoint ethnobotanical, M. nigra has popularity by presenting not only food nutritional values, but it contains several compounds with therapeutic action as an astringent, anti-inflammatory, antioxidant and antipyretic, depurative, analgesic, antiseptic, hypoglycemic and used to control the menopause, not only in the fruits, but also in other plant parts such as leaves and bark of the tree. The plant, present in large quantities in the human diet, produce a large number of natural substances, called secondary metabolites such as phenolic compounds, including flavonoids and polyphenols, which apresetam antioxidant that is, are able to inhibit or delay the process oxidative, oxidative species generated by free radical or not, delaying or preventing the oxidation of lipids and other biomolecules, thus avoiding the emergence of diseases related to oxidative stress. The objective of this study was to evaluate the potential antioxidant, antimicrobial and antihemolytic crude ethanol extract obtained by maceration of leaves of M. nigra and its fractions, the product liquid-liquid partition hexane, ethyl acetate and nbutanol. To evaluate the antioxidant activity, we used techniques such as deletion of DPPH radical species, ABTS, superoxide anion and nitric oxide, and nonoxidizing free radical species, such as HOCl. We also observed the effect of fractions oxidorredutor on catalytic activity of the enzyme horseradish peroxidase (HRP). The anti-hemolytic activity was evaluated by spectrophotometric measurement of hemoglobin released upon hemolysis without induction and inducer AAPH and HOCl. Antimicrobial activity was evaluated by microdiluições technique... (Complete abstract click electronic access below)

Thesis (D. Min.)--New Orleans Baptist Theological Seminary, 2007. / Abstract and vita. Includes final project proposal. Includes bibliographical references (leaves 94-101, 118-122, 53-56).

Thesis (D. Min.)--New Orleans Baptist Theological Seminary, 2007. / Abstract and vita. Includes final project proposal. Description based on Print version record. Includes bibliographical references (leaves 94-101, 118-122, 53-56).