In this thesis, a new traction stress based method for characterizing shear strength is investigated by carrying out a series of shear strength tests. The AWS method for the calculation of shear strength shows significant discrepancies between longitudinal and transverse specimens. The main purpose of this new traction based definition for shear strength is to demonstrate that there exists a single shear strength value regardless of specimen geometry and loading conditions. With this new approach a better correlation between shear strength values for transverse and longitudinal specimens is achieved. Special issues occur with the multi-pass welds in regards to the failure angle. The AWS equation does not account to different failure angles of the specimens, it only assumes 45o failure angle in all the cases, but the new approach takes into account the different failure angles. Finally with this method a quantitative weld sizing can be achieved for fillet welds.

This dissertation consists of two topics. One is a "Multi-pass Fiber Optic Surface Plasmon Resonance Sensor (SPR)" and the other is a "Nano-metallic Surface Plasmon Lens." Since both topics involved surface plasmon, the title of this dissertation is named "Surface plasmon coupled sensor and nanolens." For a multi-pass fiber optic SPR sensor, a fiber optic 4-pass SPR sensor coupled with a field-assist capability for detecting an extremely low concentration of charged particles is first demonstrated. The multipass feature increases the sensitivity by a factor equal to the number of passes. The field-assist feature forces charged particles/molecules to the SPR surface, increasing the sensitivity by an additional factor of about 100. Overall, the sensitivity exceeds the one-pass SPR device by a factor of about 400. A 10 pM concentration of 47 nm diameter polystyrene (PS) latex beads and 1 ?M concentration of salt dissolved in DI water were detected within a few seconds by the combined system. The equivalent index resolution for atomic size corresponding to ionized chlorine in salt is 10-8. This technique offers the potential for sensitive and fast detection of biomolecules in a solution. Secondly, a 44-pass fiber optic surface plasmon resonance (SPR) sensor coupled with a field-assist capability for measurement of refractive index change due to positive and negative ions is shown. The field-assist feature forces ions to the SPR surface, causing the SPR signal response to change which reflects a decrease or increase in refractive index depending on whether positive or negative ions are being attracted to the surface. This technique offers the potential for the sensitive detection of cations and anions in a solution. For a nano-metallic surface plasmon lens, we analyze the transmission of a normally incident plane wave through an Ag/dielectric layered concentric ring structure using finite difference time domain (FDTD) analysis. The dependency of the transmission efficiency on the refractive index in slit is studied. The numerical analysis indicates that the focusing beyond diffraction limit is found even at the extended focal length comparable to the distance of 7" from the exit plane using a circularly polarized coherent plane wave, ?=405 nm. Especially, compared to an Ag-only structure, the Ag/ LiNbO3 structure amplifies the transmission power by a factor of 6. Therefore, this Ag/dielectric layered lens has the potential for significantly higher resolution imaging and optical data storage.

The biosynthesis of integral membrane proteins (IMPs) is an essential cellular process. IMPs comprise roughly 20-30% of the protein coding genes of all organisms, nearly all of which are inserted and assembled at the endoplasmic reticulum (ER). The defining structural feature of IMPs is one or more transmembrane domains (TMDs). TMDs are typically stretches of predominately hydrophobic amino acids that span the lipid bilayer of biological membranes as an alpha helix. TMDs are remarkably diverse in terms of their topological and biophysical properties. In order to accommodate this diversity, the cell has evolved different sets of machinery that cater to particular subsets of proteins. Our knowledge of how the TMDs of IMPs are selectively recognized, chaperoned into the lipid bilayer, and assembled remains incomplete. This thesis is broadly interested in investigating how TMDs are correctly inserted and assembled at the ER. To address this the biosynthesis of multi-pass IMPs was first considered. Multi-pass IMPs contain two to more than twenty TMDs, with TMDs that vary dramatically in terms of their biophysical properties such as hydrophobicity, length, and helical propensity. The beta-1 adrenergic receptor (β1-AR), a member of the G-protein-coupled receptor (GPCR) family was established as a model substrate in an in vitro system where the insertion and folding of its TMDs could be interrogated. Assembly of β1-AR is not a straightforward process, and current models of insertion fail to explain how the known translocation machinery correctly identifies, inserts, and assembles β1-AR TMDs. An in vivo screen in mammalian cells was therefore conducted to identify additional factors which may be important for multi-pass IMP assembly. The ER membrane protein complex (EMC), a well conserved ER-resident complex of unknown biochemical function, was identified as a promising hit potentially involved in this assembly process. The complexity of working with multi-pass IMPs in an in vitro system prompted the investigation of a simpler class of proteins. Tail-anchored proteins (TA) are characterized by a single C-terminal hydrophobic domain that anchors them into membranes. Though structurally simpler compared to multi-pass IMPs, the TMDs of TA proteins are similarly diverse. We found that known TA insertion pathways fail to engage low-to-moderately hydrophobic TMDs. Instead, these are chaperoned in the cytosol by calmodulin (CaM). Transient release from CaM allows substrates to sample the ER, where resident machinery mediates the insertion reaction. The EMC was shown to be necessary for the insertion of these substrates both in vivo and in vitro. Purified EMC in synthetic liposomes catalysed insertion of its TA substrates in a fully reconstituted system to near-native levels. Therefore, the EMC was rigorously established as a TMD insertase. This key functional insight may explain its critical role in the assembly of multi- pass IMPs – which is now amenable to biochemical dissection.

Multi-pass fusion welding by a filler wire (welding electrode) is normally carried out to join thick steel sections used in most engineering applications. Welded joints in an installation, is the area of critical importance, since they are likely to contain a higher density of defects than the parent metal and their physical properties can differ significantly from the parent metal. Fusion arc welding process relies on intense local heating at a joint where a certain amount of the parent metal is melted and fused with additional metal from the filler wire. The intense local heating causes severe transient thermal gradients in the welded component and the resulting uneven cooling that follows produces a variably distributed residual stress field. In multi-pass welds, multiple thermal cycles resulted in a variably distribution of residual stress field across the weld and through the thickness. These complex thermal stresses generated in welds are undesirable but inevitable during fusion welding. Presence of such tensile residual stresses can be detrimental to the service integrity of a welded structure. In addition to a complex distribution of residual stress state, multi-pass welds also forms dendritic grain structure, which are repeatedly heated, resulting in segregation of alloying elements. Dendritic grain structure is weaker and segregation of alloying elements would result in formation of corrosion microcells as well as reduction in overall corrosion prevention due to depletion of alloying elements.

O presente trabalho investiga o comportamento da chapa de latão C268, com 0,50 mm de espessura, quando exposto ao processo de Estampagem Incremental de Chapas de Metal (Incremental Sheet Metal Forming -ISMF). Especificamente para a pesquisa, foram utilizadas as modalidades de Estampagem Incremental com Ponto Simples (Single Point Incremental Forming- SPIF) e Estampagem Incremental de Múltiplos Passes (Multi Pass Single Point Incremental Forming- MSPIF). Os experimentos foram baseados em uma geometria de tronco de pirâmide de base quadrada com 100 mm de lado e 45 mm de profundidade. Para as estratégias de estampagem foi atribuído, a estampagem helicoidal. Como resultado principal, foi verificado o ângulo de parede máximo atingido por cada processo de estampagem incremental. Adicionalmente, ensaios de tração, análise de deformações e de rugosidade da chapa de latão foram realizados. Todos os seus resultados estão detalhados na investigação. Para os parâmetros adotados nestes experimentos, o ângulo de parede obtido por SPIF foi maior do que pelo estudo proposto por MSPIF. / The present study investigates the performance of the brass plate C-268 with 0.50 mm thickness, when exposed to Incremental Sheet Metal Forming (ISMF). Specifically for research, it was used the modalities Single Point Incremental Forming (SPIF) and Multi Pass Single Point Incremental Forming (MSPIF). The experiments were based on a truncated pyramid geometry with square base 100 mm side and 45 mm depth. For forming strategies has been assigned, the helical forming. As the main outcome, it was found the maximum wall angle achieved by each process of incremental printing. In addition, tensile tests, analysis of deformation and roughness of the brass sheet were performed. All results are detailed in the investigation. For the parameters used in these experiments, the wall angle obtained by SPIF is greater than the study proposed by MSPIF.

O presente trabalho investiga o comportamento da chapa de latão C268, com 0,50 mm de espessura, quando exposto ao processo de Estampagem Incremental de Chapas de Metal (Incremental Sheet Metal Forming -ISMF). Especificamente para a pesquisa, foram utilizadas as modalidades de Estampagem Incremental com Ponto Simples (Single Point Incremental Forming- SPIF) e Estampagem Incremental de Múltiplos Passes (Multi Pass Single Point Incremental Forming- MSPIF). Os experimentos foram baseados em uma geometria de tronco de pirâmide de base quadrada com 100 mm de lado e 45 mm de profundidade. Para as estratégias de estampagem foi atribuído, a estampagem helicoidal. Como resultado principal, foi verificado o ângulo de parede máximo atingido por cada processo de estampagem incremental. Adicionalmente, ensaios de tração, análise de deformações e de rugosidade da chapa de latão foram realizados. Todos os seus resultados estão detalhados na investigação. Para os parâmetros adotados nestes experimentos, o ângulo de parede obtido por SPIF foi maior do que pelo estudo proposto por MSPIF. / The present study investigates the performance of the brass plate C-268 with 0.50 mm thickness, when exposed to Incremental Sheet Metal Forming (ISMF). Specifically for research, it was used the modalities Single Point Incremental Forming (SPIF) and Multi Pass Single Point Incremental Forming (MSPIF). The experiments were based on a truncated pyramid geometry with square base 100 mm side and 45 mm depth. For forming strategies has been assigned, the helical forming. As the main outcome, it was found the maximum wall angle achieved by each process of incremental printing. In addition, tensile tests, analysis of deformation and roughness of the brass sheet were performed. All results are detailed in the investigation. For the parameters used in these experiments, the wall angle obtained by SPIF is greater than the study proposed by MSPIF.

O presente trabalho investiga o comportamento da chapa de latão C268, com 0,50 mm de espessura, quando exposto ao processo de Estampagem Incremental de Chapas de Metal (Incremental Sheet Metal Forming -ISMF). Especificamente para a pesquisa, foram utilizadas as modalidades de Estampagem Incremental com Ponto Simples (Single Point Incremental Forming- SPIF) e Estampagem Incremental de Múltiplos Passes (Multi Pass Single Point Incremental Forming- MSPIF). Os experimentos foram baseados em uma geometria de tronco de pirâmide de base quadrada com 100 mm de lado e 45 mm de profundidade. Para as estratégias de estampagem foi atribuído, a estampagem helicoidal. Como resultado principal, foi verificado o ângulo de parede máximo atingido por cada processo de estampagem incremental. Adicionalmente, ensaios de tração, análise de deformações e de rugosidade da chapa de latão foram realizados. Todos os seus resultados estão detalhados na investigação. Para os parâmetros adotados nestes experimentos, o ângulo de parede obtido por SPIF foi maior do que pelo estudo proposto por MSPIF. / The present study investigates the performance of the brass plate C-268 with 0.50 mm thickness, when exposed to Incremental Sheet Metal Forming (ISMF). Specifically for research, it was used the modalities Single Point Incremental Forming (SPIF) and Multi Pass Single Point Incremental Forming (MSPIF). The experiments were based on a truncated pyramid geometry with square base 100 mm side and 45 mm depth. For forming strategies has been assigned, the helical forming. As the main outcome, it was found the maximum wall angle achieved by each process of incremental printing. In addition, tensile tests, analysis of deformation and roughness of the brass sheet were performed. All results are detailed in the investigation. For the parameters used in these experiments, the wall angle obtained by SPIF is greater than the study proposed by MSPIF.

Tire performance over soft soil influences the performance of off-road vehicles on soft soil, as the tire is the only force transmitting element between the off-road vehicles and soil during the vehicle operation. One aspect of the tire performance over soft soil is the tire tractive performance on soft soil, and it attracts the attention of vehicle and geotechnical engineers. The vehicle engineer is interested in the tire tractive performance on soft soil because it is related to vehicle mobility and energy efficiency; the geotechnical engineer is concerned about the soil compaction, brought about by the tire traffic, which accompanies the tire tractive performance on soft soil. In order to improve the vehicle mobility and energy efficiency over soft soil and mitigate the soil compaction, it's essential to develop an in-depth understanding of tire tractive performance on soft soil. This study has enhanced the understanding of tire tractive performance on soft soil and promoted the development of terramechanics and tire model parameterization method through experimental tests. The experimental tests consisted of static tire deflection tests, static tire-soil tests, soil properties tests, and dynamic tire-soil tests. The series of tests (test program) presented herein produced parameterization and validation data that can be used in tire off-road traction dynamics modeling and terramechanics modeling. The 225/60R16 97S Uniroyal (Michelin) Standard Reference Test Tire (SRTT) and loamy sand were chosen to be studied in the test program. The tests included the quantification or/and measurement of soil properties of the test soil, pre-traffic soil condition, the pressure distribution in the tire contact patch, tire off-road tractive performance, and post-traffic soil compaction. The influence of operational parameters, e.g., tire inflation pressure, tire normal load, tire slip ratio, initial soil compaction, or the number of passes, on the measurement data of tire performance parameters or soil response parameters was also analyzed. New methods of the rolling radius estimation for a tire on soft soil and of the 3-D rut reconstruction were developed. A multi-pass effect phenomenon, different from any previously observed phenomenon in the available existing literature, was discovered. The test data was fed into optimization programs for the parameterization of the Bekker's model, a modified Bekker's model, the Magic Formula tire model, and a bulk density estimation model. The modified Bekker's model accounts for the slip sinkage effect which the original Bekker's pressure-sinkage model doesn't. The Magic Formula tire model was adapted to account for the combined influence of tire inflation pressure and initial soil compaction on the tire tractive performance and validated by the test data. The parameterization methods presented herein are new effective terramechanics model parameterization methods, can capture tire-soil interaction which the conventional parameterization methods such as the plate-sinkage test and shear test (not using a tire as the shear tool) cannot sufficiently, and hence can be used to develop tire off-road dynamics models that are heavily based on terramechanics models. This study has been partially supported by the U.S. Army Engineer Research and Development Center (ERDC) and by the Terramechanics, Multibody, and Vehicle (TMVS) Laboratory at Virginia Tech. / Doctor of Philosophy / Big differences exist between a tire moving in on-road conditions, such as asphalt lanes, and a tire moving in off-road conditions, such as soft soil. For example, for passenger cars commonly driven on asphalt lanes, normally, the tire inflation pressure is suggested to be between 30 and 35 psi; very low inflation pressure is also not suggested. By contrast, for off-road vehicles operated on soft soil, low inflation pressure is recommended for their tires; the inflation pressure of a tractor tire can be as low as 12 psi, for the sake of low post-traffic soil compaction and better tire traction. Besides, unlike the research on tire on-road dynamics, the research on off-road dynamics is still immature, while the physics behind the off-road dynamics could be more complex than the on-road dynamics. In this dissertation, experimental tests were completed to study the factors influencing tire tractive performance and soil behavior, and model parameterization methods were developed for a better prediction of tire off-road dynamics models. Tire or vehicle manufacturers can use the research results or methods presented in this dissertation to offer suggestions for the tire or vehicle operation on soft soil in order to maximize the tractive performance and minimize the post-traffic soil compaction.

Our society is heavily and intrinsically dependent on energy transformation and usage. In a world scenario where resources are being depleted while their demand is increasing, it is crucial to optimize every process. During the last decade the concept of energy efficiency has become a leitmotif in several fields and has directly influenced our everyday life: from light bulbs to airplane turbines, there has been a general shift from pure performance to better efficiency. In this vein, we focus on the mobility and tractive efficiency of off-road vehicles. These vehicles are adopted in military, agriculture, construction, exploration, recreation, and mining applications and are intended to operate on soft, deformable terrain. The performance of off-road vehicles is deeply influenced by the tire-soil interaction mechanism. Soft soil can drastically reduce the traction performance of tires up to the point of making motion impossible. In this study, a tire model able to predict the performance of rigid wheels and flexible tires is developed. The model follows a semi-empirical approach for steady-state conditions and predicts basic features, such as the drawbar pull, the driving torque and the lateral force, as well as complex behaviors, such as the slip-sinkage phenomenon and the multi-pass effect. The tractive efficiency of different tire-soil configurations is simulated and discussed. To investigate the handling and the traction efficiency, the tire model is implemented into a four-wheel vehicle model. Several tire geometries, vehicle configurations (FWD, RWD, AWD), soil types, and terrain profiles are considered to evaluate the performance under different simulation scenarios. The simulation environment represents an effective tool to realistically analyze the impact of tire parameters (size, inflation pressure) and torque distribution on the energy efficiency. It is verified that larger tires and decreased inflation pressure generally provide better traction and energy efficiency (under steady-state working conditions). The torque distribution strategy between the axles deeply affects the traction and the efficiency: the two variables can't clearly be maximized at the same time and a trade-off has to be found. / Ph. D.

With the growth in pipeline installations all over the world, there is a great demand for highly productive and robust welding systems. Mechanised pipe welding has been developed over the last 50 years and the present focus is towards development of automated pipeline welding systems. Pipeline welding automation is aimed at reducing costs and improving the installation quality. To attain fully automated pipe welding systems there is a need to rely on sensors and controls systems to mimic human like capabilities, such as visual inspection, in real time. The key aim of this work is to develop and evaluate methods of automatic assessment of weld bead shape and quality during narrow gap GMAW of transmission pipelines. This implies that the measured bead profile will be assessed to determine whether the bead shape will cause defects when the subsequent pass is deposited. Different approaches have been used to conquer the challenge that is emulating human reasoning, all with different objectives in mind. In spite of extensive literature research performed, very little information was found concerning the real time determination and assessment of bead shape quality and none of it was reported to be applied successfully to the pipeline industry. Despite the continuous development of laboratory laser vision systems commercial ones have been on the market for decades, some specifically developed for the welding application. Laser vision sensor systems provide surface profile information, and are the only sensors which can satisfactorily measure bead profile on a narrow groove. In order to be able to use them to automatically assess weld bead shape and quality, a deep understanding of their characteristics and limitations needs to be achieved. Once that knowledge was attained it was then applied to determine the best sensor configuration for this purpose. After that the development of human like judgment algorithms were developed to accomplish the aim that was set. Empirical rules were obtained from an experienced welder regarding the acceptability of bead shapes and were then applied in the developed system with good results. To scientifically evaluate and determine the rules to use in this system, further experiments would be required. The output of the system developed showed very accurate, reliable and consistent results that were true to the external measurements and comparisons performed. The developed system has numerous applications in the pipeline industry and it could easily be implemented on commercial systems.