There are several reasons for imaging a single, developing embryo from multiple view points. The embryo is a complex biomechanical system and morphogenesis movements in one region typically produce motions in adjacent areas. Multi-view imaging can be used to observe morphogenesis and gain a better understanding of normal and abnormal embryo development. The system would allow the embryo to be rotated to a specific vantage point so that a particular morphogenetic process may be observed clearly. Moreover, a multi-view system can be used to gather images to create an accurate three-dimensional reconstruction of the embryo for computer simulations. The scope of this thesis was to construct an apparatus that could capture multi-view images for these applications. A multi-view system for imaging live Drosophila melanogaster embryos, the first of its kind, is presented. Embryos for imaging are collected from genetically modified Drosophila stocks that contain a green fluorescing protein (GFP), which highlights only specific cell components. The embryos are mounted on a wire that is rotated under computer control to desired viewpoints in front of the objective of a custom-built confocal microscope. The optical components for the orizontallyaligned microscope were researched, selected and installed specifically for this multi-viewing apparatus. The multiple images of the stacks from each viewpoint are deconvolved and collaged so as to show all of the cells visible from that view. The process of rotating and capturing images can be repeated for many angles over the course of one hour. Experiments were conducted to verify the repeatability of the rotation mechanism and to determine the number of image slices required to produce a satisfactory image collage from each viewpoint. Additional testing was conducted to establish that the system could capture a complete 360° view of the embryo, and a time-lapse study was done to verify that a developing embryo could be imaged repeatedly from two separate angles during ventral furrow formation. An analysis of the effects of the imaging system on embryos in terms of photo-bleaching and viability is presented.

There are several reasons for imaging a single, developing embryo from multiple view points. The embryo is a complex biomechanical system and morphogenesis movements in one region typically produce motions in adjacent areas. Multi-view imaging can be used to observe morphogenesis and gain a better understanding of normal and abnormal embryo development. The system would allow the embryo to be rotated to a specific vantage point so that a particular morphogenetic process may be observed clearly. Moreover, a multi-view system can be used to gather images to create an accurate three-dimensional reconstruction of the embryo for computer simulations. The scope of this thesis was to construct an apparatus that could capture multi-view images for these applications. A multi-view system for imaging live Drosophila melanogaster embryos, the first of its kind, is presented. Embryos for imaging are collected from genetically modified Drosophila stocks that contain a green fluorescing protein (GFP), which highlights only specific cell components. The embryos are mounted on a wire that is rotated under computer control to desired viewpoints in front of the objective of a custom-built confocal microscope. The optical components for the orizontallyaligned microscope were researched, selected and installed specifically for this multi-viewing apparatus. The multiple images of the stacks from each viewpoint are deconvolved and collaged so as to show all of the cells visible from that view. The process of rotating and capturing images can be repeated for many angles over the course of one hour. Experiments were conducted to verify the repeatability of the rotation mechanism and to determine the number of image slices required to produce a satisfactory image collage from each viewpoint. Additional testing was conducted to establish that the system could capture a complete 360° view of the embryo, and a time-lapse study was done to verify that a developing embryo could be imaged repeatedly from two separate angles during ventral furrow formation. An analysis of the effects of the imaging system on embryos in terms of photo-bleaching and viability is presented.

For many years, light fields have been a unique way of capturing a scene. By using a particular set of optics, a light field camera is able to, in a single moment, take images of the same scene from multiple perspectives. These perspectives can be used to calculate the scene geometry and allow for effects not possible with standard photographs, such as refocus and the creation of novel views.Neural style transfer is the process of training a neural network to render photographs in the style of a particular painting or piece of art. This is a simple process for a single photograph, but naively applying style transfer to each view in a light field generates inconsistencies in coloring between views. Because of these inconsistencies, common light field effects break down.We propose a style transfer method for light fields that maintains consistencies between different views of the scene. This is done by using warping techniques based on the depth estimation of the scene. These warped images are then used to compare areas of similarity between views and incorporate differences into the loss function of the style transfer network. Additionally, this is done in a post-training fashion, which removes the need for a light field training set.

La protection des documents fiduciaires et identitaires contre la fraude exige le développement d’outils de contrôle fondés sur des effets visuels sans cesse renouvelés, difficiles à contrefaire (même pour un expert ... de la contrefaçon !). Ce projet de recherche s’inscrit dans cette problématique et vise à apporter des solutions originales via l’impression de supports diffusants d’une part, et le développement de modèles de rendu visuel d’autre part. Les effets visuels recherchés sont des ajustements de couleurs entre les deux faces d’un imprimé lorsque celui-ci est observé par transparence devant une source lumineuse. Pour obtenir facilement des ajustements de couleurs quelles que soient les couleurs visées, il est capital d’avoir un modèle à disposition, permettant de calculer les quantités d’encre à déposer. Un modèle doit être capable de prédire les facteurs spectraux de réflexion et de transmission du support imprimé en décrivant les phénomènes de diffusion optique présents en pratique dans les couches d’encre et le support. Nous nous intéressons plus particulièrement aux imprimés translucides contenant des couleurs en demi-ton des deux côtés de la surface avec pour objectif de prédire le rendu visuel pour diverses configurations d’observation. Pour cela, nous proposons une nouvelle approche basée sur l’utilisation de matrices de transfert de flux pour prédire les facteurs spectraux de réflexion et de transmission des imprimés lorsqu’ils sont éclairés simultanément des deux côtés. En représentant le comportement optique des différents composants d’un imprimé par des matrices de transfert, la description des transferts de flux entre ces composantes s’en trouve simplifiée. Ce cadre mathématique mène à la construction de modèles de prédiction de couleurs imprimées en demi-ton sur des supports diffusants. Nous montrons par ailleurs que certains modèles existants, comme le modèle de Kubelka-Munk ou encore le modèle de Clapper-Yule, peuvent également être formulés en termes de matrices de transfert. Les résultats obtenus avec les modèles proposés dans ce travail mettent en évidence des qualités de prédiction équivalentes, voire supérieures, à celles qu’on retrouve dans l’état de l’art, tout en proposant une simplification de la formulation mathématique et de la description physique des échanges de flux. Cette simplification fait de ces modèles des outils de calcul qui s’utilisent très facilement, notamment pour la détermination des quantités d’encre à déposer sur les deux faces de l’imprimé afin d’obtenir des ajustements de couleurs / The protection of banknotes or identity documents against counterfeiting demands the development of control tools based on visual effects that are continuously renewed. These visual effects become thus difficult to counterfeit even by an expert forger ! This research tries to deal with that issue. Its objective is to bring new solutions using on the one side, the printing of diffusing materials, and on the other side the development of visual rendering models that can be observed. The visual effects that are sought-after are the color matching on both sides of a printed document when observed against thelight. To easily obtain a color matching, whatever the colors that are aimed for, it is essential to have a model that helps in calculating the quantity of ink to be left on the document. A model must be used to predict the spectral reflectance and the transmittance factors of the printed document by describing the phenomena of optical diffusion really present in the ink layers and in the document. We shall focus our interest especially on translucent printed documents that have halftone colors on both sides. Our goal here is to predict the visual rendering in different configurations of observation. To that end, we are offering a new approach based on the use of flux transfer matrices to predict the spectral reflectance and transmittance factors of prints when they are simultaneously lit up on both sides. By representing with transfer matrices the optical behavior of the different components present in a printed document, we see that the description of flux transfer between these elements is thus simplified. This mathematical framework leads to the construction of prediction models of halftone printed colors on diffusing materials. We also show that some existing models, such as the Kubelka-Munk or the Clapper-Yule models, can also be formulated in transfer matrices terms. The results that we get with the models used in this work make apparent identical prediction quality and in some cases even better ones to the ones found in the state of the art, while offering a simplification of the mathematical formulation and the physical description of the flux transfer. This simplification thus transforms these models into calculation tools that can easily be used especially for the choice of quantities of ink that must be left on both sides of the document in order to obtain color matching