Thesis (Ph. D.)--Case Western Reserve University, 2007. / [School of Medicine] Department of Neurosciences. Includes bibliographical references. Available online via OhioLINK's ETD Center.

Most of genes contained in a genome have been shown to exist in forms of families; however, little is known about their variation and evolution during the course of genome evolution. The present study shows that the numbers of the genes of the NBS-LRR-encoding gene family vary extremely significantly among different lines or cultivars of a species and among related species from the same genus. This suggests that plant genetics and evolution depend on not only gene sequence variation, but also the number of genes in multigene families. This study has further revealed that the variation of number of genes in the gene family in the Gossypium species is affected significantly not only by genome size variation, polyploidization and natural selection, but also by domestication/breeding. There is a positive correlation (P less than or equal to 0.05) between genome size and number of genes in the family, suggesting that species with larger genomes tend to have more NBS-LRR-encoding genes. It was observed that natural polyploids have significantly larger numbers of genes in the family and larger genomes than the artificial polyploids of their putative diploid ancestors. This indicates that polyploidization, perhaps post-polyploidization as well, either led to the loss of the genes in a gene family or slowed the process of gene number increase after polyploidization. It was shown that cultivated cottons have significantly more NBSLRR- encoding genes than wild species at both diploid and polyploidy levels. This result indicates that plant breeding likely allows accumulation of NBS-LRR-encoding genes that potentially provide resistance to pathogens. Therefore, plant breeders have selected not only for favorable alleles and favorable allele combinations, but also for the number of genes. Finally, difference (P less than or equal to 0.001) was found in number of genes in the NBS-LRR-encoding gene family among the species native to different geographical regions, suggesting that natural selection has played an important role in the variation in number of genes in the NBS-LRR-encoding gene family. The gene members that are favorable for fitness at the time are selected and accumulated in the genomes, but those that are not favorable for fitness at the time are lost in natural selection. As this is the first study in the field, further studies remain. These include, but not limited to, the universality of the findings in plants and animals, the universality of the findings in different gene families, genetics of the gene family size variation, relationship between the gene family size variation and phenotypic variation, gene family size variation and breeding, etc. Nevertheless, the findings obtained from this study are sufficient to shed light on many fundamental questions in biology, diversity and complexity of plants and animals.

No description available.

Thesis (Ph. D.)--Cornell University, 1989. / Vita. Includes bibliographical references.

The post-genomics era has put at the disposal of modern plant breeders an endless list of genetic building blocks for the design of new biotechnological crops. After a first wave of single-gene transgenic with controversial public acceptance, genomic information and technology is paving the way for increasingly complex designs based in multiple gene engineering. Those designs aiming at the production of inexpensive health-promoting compounds are most likely to be welcomed by consumers. In this project we plan to develop new multigene assembling tools. During this PhD, a standardized collection of interchangeable genetic parts (including promoters, CDS, P-DNAs, etc) and vectors will be developed. The collection, inspired in Synthetic Biology standards, will be made easy-to-assemble in an interchangeable, semi-idempotent and seamless fashion by the addition of flanking recognition sites of type IIS Restriction endonucleases. The construction of the collection will facilitate multigene engineering and will constitute a first step towards enabling Synthetic Biology in plants. / Sarrión Perdigones, MA. (2014). Design and development of modular DNA assembly tools for Multigene Engineering and Synthetic Biology in Plants [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/35399 / TESIS

Unbonded post-tensioned concrete block (UPCB) shear walls are an effective seismic force resisting system due to their ability to contain expected damage attributed to their self-centering capabilities. A few design procedures were proposed to predict the in-plane flexural response of UPCB walls, albeit following only basic mechanics and/or extensive iterative methods. Such procedures, however, may not be capable of capturing the complex nonlinear relationships between different parameters that affect UPCB walls’ behavior or are tedious to be adopted for design practice. In addition, the limited datasets used to validate these procedures may render their accuracy and generalizability questionable, further hindering their adoption by practitioners and design standards. To address these issues, an experimentally-validated nonlinear numerical model was adopted in this study and subsequently employed to simulate 95 UPCB walls with different design parameters to compensate for the lack of relevant experimental data in the current literature. Guided by mechanics and using this database, an evolutionary algorithm, multigene genetic programming (MGGP), was adopted to uncover the relationships controlling the response of UPCB walls, and subsequently develop simplified closed-form wall behavior prediction expressions. Specifically, through integrating MGGP and basic mechanics, a penta-linear backbone model was developed to predict the load-displacement backbone for UPCB walls up to 20% strength degradation. Compared to existing predictive procedures, the prediction accuracy of the developed model and its closed-form nature are expected to enable UPCB wall adoption by seismic design standards and code committees. / Thesis / Master of Applied Science (MASc)

Diss. (sammanfattning) Uppsala : Univ., 2003. / Härtill 4 uppsatser.

Diss. (sammanfattning) Umeå : Univ., 2008. / Härtill 5 uppsatser.

Thesis (Ph. D.)--University of Washington, 1999. / Vita. Includes bibliographical references (leaves 111-124).

In temperate zone, especially Europe and North America, is the genus Geastrum very well inspected group of Gasteromycetes with long lasting and comprehensive history of research. Our knowledge of the diversity in other areas of distribution, especially in tropics, is more than limited. The aim of this diploma thesis is to contribute for cognition of diversity of this genus in Panama, Costa Rica and Colombia I have collected 86 samples belonging to genus Geastrum during three expeditions to Panama (Chiriqué province) in the years 2015, 2016, 2017. I have characterized those based on morphological and molecular data. I have also studied morphology of 40 borrowed herbarium specimens from Costa Rica, Colombia and Panama. I have successfully sequenced four locuses of DNA from my own samples (ITS, LSU, RPB1 and ATP6). This method had lover efficiency in herbarium specimens due to present contaminations, age of specimens or probably badly stored. Molecular phylogenetic analysis was carried out by Bayesian method and its results have in agreement with morphologic traits shown many lineages new for Panama and moreover at least four new species completely new to science. Variability in species complexes (e.g. G. lageniforme, G. saccatum, G. velutinum) is presented in more detail with emphasis to lineages...