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1	Spatial mapping of motile cilia proteins in respiratory and female reproductive tissues Bertilsson, Filippa January 2024 (has links) Motile cilia play critical roles in the human body, including expelling mucus from the lungs and facilitating the transport of oocytes and sperm through the fallopian tubes. Understanding the complex structure and motility of cilia, as well as the diseases associated with them, is of big importance. This study investigates the proteins expressed in ciliated cells from both respiratory and reproductive tissues using multiplex immunofluorescence. We determined the subcellular localization of 134 proteins in the fallopian tube, endometrium, cervix, nasopharynx, and bronchus, focusing on five subcellular regions: the cilia tip, transition zone, basal body, cytoplasm, and nucleus. This analysis was conducted using an automated image analysis method developed specifically for this project. Our findings revealed a high correlation in protein expression across all tissues, although several proteins exhibited distinct expression patterns between different tissues. Notably, the fallopian tube showed a higher correlation with the nasopharynx and bronchus than with the endometrium and cervix. Within these proteins, six gene clusters were identified, with the two largest clusters being strongly associated with ciliary structure. This study enhances our understanding of motile ciliary structures and ciliated cells, identifying key proteins for further research into cilia motion, function, and related diseases. Motile cilia multiplex immunofluoresence respiratory tissues female reproductive tissues fallopian tube endometrium cervix nasopharynx bronchus spatial biology protein expression image analysis cilia related disease Biochemistry and Molecular Biology Biokemi och molekylärbiologi Cell Biology Cellbiologi Immunology Immunologi Biomedical Laboratory Science/Technology

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Spatial mapping of motile cilia proteins in respiratory and female reproductive tissues