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Effects of time specific F-strain Mycoplasma gallisepticum inoculation overlays on pre-lay ts-11-strain Mycoplasma gallisepticum inoculation on performance, egg, blood, and visceral characteristics of commercial egg laying hensVance, Elizabeth Anne Middleton, January 2007 (has links)
Thesis (M.S.)--Mississippi State University. Department of Poultry Sciences. / Title from title screen. Includes bibliographical references.
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Untersuchung zur kohlenstoffabhängigen Wasserstoffperoxidproduktion und Virulenz in Mycoplasma pneumoniae und Mycoplasma genitalium / Analyses about the carbon source dependent hydrogen peroxide formation and the virulence in Mycoplasma pneumoniae and Mycoplasma genitaliumSchmeisky, Arne Gunnar 18 April 2013 (has links)
Die beiden Humanpathogene M. genitalium und M. pneumoniae nutzen als wichtigen Pathogenitätsfaktor die Produktion von reaktivem Wasserstoffperoxid. Dabei konnte gezeigt werden, dass die Glyzerin-3-Phosphat-Oxidase (GlpD) das Schlüsselenzym für die Produktion von H2O2 und die Verbindung von Glyzerin- und GPC-Stoffwechsel mit der Glykolyse ist.
In dieser Arbeit sollte der Einfluss verschiedener C-Quellen auf die Peroxidproduktion untersucht werden. Dabei konnte gezeigt werden, dass eine Reihe von Mutanten im Glyzerinstoffwechselweg dazu in der Lage ist, Glyzerin zu verwerten, jedoch nur bei Anwesenheit eines PTS-Zuckers. Weiterhin konnte nachgewiesen werden, dass in M. genitalium neben GlpD ein weiteres Enzym vorhanden sein muss, welches mit Glukose Wasserstoffperoxid bilden kann. Außerdem konnte gezeigt werden, dass die Expression verschiedener Gene aus den peroxidproduzierenden Stoffwechselwegen sowohl von der C-Quelle als auch von anderen Enzymen dieser Reaktionsreihen abhängig ist.
Neben M. genitalium G37 und M. pneumoniae M129 wurde in dieser Studie ein klinisches Isolat näher charakterisiert. Auch dieser Stamm zeigte einen ungewöhnlichen Phänotyp bezüglich der Peroxidproduktion. Eine Analyse der Genomsequenz erwies eine Reihe Mutationen im Vergleich zu einem weiteren Referenzstamm M. pneumoniae FH (ATCC 15531), die möglicherweise für diesen Phänotyp verantwortlich sind.
In weiteren Experimenten konnte in vitro gezeigt werden, dass Mpn244 Diadenylatzyklaseaktivität aufweist und der durch dieses Enzym produzierte Botenstoff c-di-AMP auch in vivo in M. pneumoniae M129 vorkommt. Die genaue Funktion von c-di-AMP muss jedoch in weiteren Studien genauer charakterisiert
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MYCOPLASMA AGASSIZII IN THE SONORAN POPULATION OF THE DESERT TORTOISE IN ARIZONAJones, Cristina Ann January 2008 (has links)
Upper Respiratory Tract Disease (URTD), caused by the pathogens Mycoplasma agassizii and M. testudineum, has been documented in the desert tortoise (Gopherus agassizii). Although URTD was identified as a putative agent that led to federal listing of the Mojave population of the desert tortoise, little is known about this disease in the Sonoran population of the desert tortoise. The purpose of this study was to determine: 1) the prevalence of URTD across an urban gradient in Greater Tucson, Arizona, 2) the relationship between URTD and captive and free-ranging tortoises in Mohave, Maricopa, and Pima counties in Arizona, and 3) the effects of URTD on desert tortoise home range size and winter temperature selection.
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Analysis of the Mycoplasma hominis hsp70 gene and development of a PCR ELISA assay.Shearer, Nicollette. 23 December 2013 (has links)
Mycoplasmas conform most closely with the theoretical concept of 'minimum cells', existing as
the smallest, free-living organisms capable of self-replication. They survive as parasites of plants,
insects, animals or humans, with the most common human colonising species being Mycoplasma
hominis. M. hominis has been characterised as a human pathogen responsible for a variety of
infections, which pose a significant threat particularly to immunocompromised patients and
neonates. However little has been elucidated about the cell physiology and molecular structure
of this organism. Of interest to this study were the investigation of the heat shock response of
M. hominis and the diagnostic assays used for its detection.
The heat shock response is a ubiquitous physiological feature of all organisms and displays
unprecedented conservation. This phenomenon is particularly evident in the 70 kDa family of
heat shock proteins (hsp70) which exhibits a high degree of homology between different species.
The hsp70 gene from M. hominis was cloned and preliminary partial sequencing indicated the
similarity with other hsp70 homologs. The regulation of hsp70 expression at the transcriptional
and translational levels was investigated. The level of hsp70 mRNA was found to increase
correspondingly in response to heat shock, more visibly than the level of hsp70 protein.
However imrnunochemical studies of the M. hominis hsp70 translation product demonstrated
further the homology with other species.
To facilitate rapid diagnosis of M. hominis infections, a PCR ELISA diagnostic assay was
developed and optimised. The amplification of a conserved region of the M. hominis 16S rRNA
gene was linked to subsequent hybridisation to an appropriate capture probe in a microtiter plate
format. The sensitivity of the assay was comparable to other molecular assays although the PCR
ELISA produces more rapid results and is less labour intensive. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1998.
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Molecular characterisation of Mycoplasma mycoides subsp. mycoides SC /Persson, Anja M., January 2002 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2002. / Härtill 4 uppsatser.
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Identification of Mycoplasma gallisepticum antigens with diagnostic and protective properties /Czifra, György. January 1900 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 6 uppsatser.
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Models for infections in immunodeficiency /Berglöf, Anna, January 2002 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2002. / Härtill 5 uppsatser.
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Bacterial enzymes in thymidylate synthesis : molecular characterization of thymidine kinase and thymidylate kinase in Ureaplasma urealyticum and Bacillus anthracis; implications for antibacterial therapy /Carnrot, Cecilia, January 2006 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniversitet, 2006. / Härtill 5 uppsatser.
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Characterization and strain distribution of multicopy allelic variants of the M. fermentans membrane lipoprotein gene, p57 /Lu, Tonghua. January 1998 (has links)
Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / "May 1998." Typescript. Vita. Includes bibliographical references (leaves 138-147). Also available on the Internet.
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The essential role of macrophages and TLR signaling in the host response to Mycoplasma pneumoniaeLai, Jen-Feng. January 2009 (has links) (PDF)
Thesis (Ph.D.)--University of Alabama at Birmingham, 2009. / Title from PDF title page (viewed on July 14, 2010). Includes bibliographical references.
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