The aim of the thesis was development of an innovative treatment of bone defects. Human multipotent mesenchymal stromal cells (MSC) play a crucial role in bone healing. Clinical applications of MSC require large amount of cells, which could be obtained by autologous expansion of MSC harvested from bone marrow. As a first step, the standard protocol of MSC expansion based on αMEM medium and fetal bovine serum (FBS) was used. Experiments replacing FBS by pooled human serum (HS) in the culture medium concluded in patenting of a new MSC cultivation protocol (EU 1999250, CR 301141). This one-step cultivation protocol and xenogeneic protein-free cultivation medium is based on CellGro® for Hematopoietic Cells' Medium, HS, human recombinant growth factors, dexamethasone, insulin and ascorbic acid. The preclinical in vitro and in vivo experiments with MSC from both expansion protocols were carried out. Fibrillar polylactic scaffolds were seeded with MSC, cultured, differentiated and implanted in immunodeficient mice (NOD/LtSz-Rag1-). Bone-like mineralized tissue containing vessels was observed. The MSC cultured according to patented method were classified as Advanced-therapy Medicinal Product and has to fulfil the European Medicines Agency regulations to enter the clinical trials. Nevertheless the use of MSC seems...

The damage of ocular surface represents one of the most common causes of decreased quality of vision or even blindness. If the injury is extensive and includes the region of limbus, niche of limbal stem cells (LSC), LSC deficiency occurs and the natural corneal regeneration is stopped. Conjunctival epithelium migrates into the injured area. Neovascularization, local inflammation and corneal opacity occur. Corneal transplantation is an insufficient treatment in such case. If the injury is bilateral, the allogenic limbal graft or LSC transplantation is required. In such cases systemic immunosuppressive drugs with many negative side-effects must be administered. The search for an adequate autologous substitution is important for avoid immunosuppressive medication. Mesenchymal stem cells (MSC) represent a perspective substitution for the reason of their immunomodulatory properties and the capability to differentiate in many cell types. There is possibility to isolate sufficient number of these cells from adipose tissue or bone marrow which are relatively easily accessible. Our goal was to observe local production of cytokines and other molecules which are present in inflammatory reaction after the chemical burn of the murine cornea and after the treatment with stem cells growing on nanofiber scaffold....

This Diploma Thesis is dedicated to stress analysis of carrier swap bodies Mega 25 with maximum technical weight 27 000 kg and has been worked out in corporation with ZDT Nové Veselí company. The aim of this work is to stress analysis of trailer in various burdensome conditions. The first section of the Thesis is occupied with original design and burdensome condition. Then, according to these states gained strength to control the finite element method, implement and then modify the design including design of new drawbar and then create selected construction drawings prepared.

This diploma thesis deals with FEM analysis and optimization of carriage for top drive system of drilling rig Bentec 350 AC and it is defined as a development contract for company MND Drilling & Services a.s. Intensive drilling leads to damages and repeated occurrence of fatigue cracks on carriage especially along edges of welded ribs in their heat-affected zone. The aim of this thesis is to draft construction improvements of carriage which leads to elimination of damages. Realization of this modifications depends on correct definition of boundary conditions, analysis of damage occurrence as well as verification of carriage drafts with finite element method.

This diploma thesis is engaged in the analysis of a safety mechanism, which is a part of a truck trailer. This mechanism provides a safe and firm connection of a container to the trailer. Primarily abroll containers according to German standard DIN 30 722 are being transported. The introductory part of the thesis deals with the current traffic condition and the accident rate in the Czech Republic. Legislative requirements for safety elements of trailer technology for road transportation are further defined. They are followed by a recherche of industrial solutions for securing mechanisms. Consequently calculations to obtain necessary securing force were performed. An analysis of the mechanism kinematics was carried out then. A structural check of selected mechanism elements was carried out following the kinematics check. Further, an optimalization of a transom design was carried out followed by another structural check. The structural checks were carried out using both the analytical method and the finite element method. In conclusion design documentation was elaborated. This diploma thesis was elaborated in cooperation with the SVAN Chrudim s.r.o.

This diploma thesis is focused on container safety mechanism which is a part of truck trailers. It designs pneumatic – mechanical safety mechanism suitable for safe transport of container with driving drum which are produced according to German standard DIN 30 722. It concerns containers with total weight of 22 tons. The first part of this thesis is focused on evaluation of possible load by common operating conditions. The technique of mechanism design is introdiced in several developing stages from original solution to final version. The final version processes all force and kinetic aspects. Selected parts of safety mechanism are controlled from solidity perspective. Some calculations and analysis were processed both by convention method and method of final elements on PC. Thesis was created in close cooperation with company SVAN Chrudim, s.r.o.

The main objective of this thesis is the study of the optical and optoelectronic properties of organic semiconducting materials. At the beginning of this thesis is shown the growing interest in these materials and their practical application. The theoretical part presents the current state of knowledge of studied problematic related to optical phenomena and the transport of charge carriers in organic semiconductors, photochormic phenomena of reversible molecular switch and charge transfer in DNA biopolymeric matrix. Experimental section, which consists of selected author’s publications, includes study of the charge transport, photochromic reversible switching and the role of dopant in pi-conjugated organic polymer system. Conductive and semiconductive characteristics of the DNA biopolymer have been aslo studied. Conductive and photoconductive techniques together with measurement of the optical characteristics have been used for organic semiconductors characterization.

The aim of present work is cultivation of probiotic bacteria L. acidophilus, B. breve and B. longum in such a way that the culture forms cells clusters or comprehensive biofilm on the variety of free carriers. Biofilm formation of L. acidophilus on the silica from point of view bile and acid tolerance in gastrointestinal tract was studied. While the number of living cells in planktonic form (planktonic form) at pH 1 fell by 30 %, the viability of the biofilm cells was maintained to 90 % under the same environmental conditions. The biofilm culture showed also the protection against environment contained bile. Furthermore, the possibilities of drying procedures of biofilm cultures used as commercial technologies in pharmaceutical industry were studied. The comparison of freeze-drying and fluidization bed drying showed, that freeze-drying is more suitable method, which is able to achieve higher amount of viable cells after drying than fluidization bed drying. The effectivity of freeze-drying method is dependent on the selection of suitable cryprotective medium. In this case, about 90 % higher viability after freeze drying was achieved in comparison with fluidization bed drying. Finally, the industrial processing of probiotic strains into the solid dosage form was studied. Tablets should be produced at hardness between 70 and 90 N and water activity of tablet mixture can be maintained below 0.3. Consequently, the drying step of the tablets in a hermetically closed space with at least 10 % of silica gel must be ensured. Thereafter, the tablets contain (5.4 ± 0.7)109 viable cells after 6 months of drying process. Capsule production technology has no significant effect on the cell‘s viability during production. The triplex blistering foil for primary blistering of probiotic capsules was chosen. The triplex foil, which has low values of water vapour transition rate (0.07 g H2O / (m2 × day) and oxygen transition rate (0.01 cm3/m2 × day), was chosen. Other studied blistering foils commonly used in the pharmaceutical industry are not suitable for long storage of solid dosage forms contained probiotics.

Práce se zabývá návrhem magnetoreologické (MR) vzpěry vibroizolačního systému (VIS) pro kosmický nosič. V rešeršní části jsou popsány vybrané VIS a vzpěry těchto systémů, které byly v kosmických nosičích využity v minulosti. Každá z těchto vzpěr obsahující kapalinu byla těsněna pomocí statických těsnění a pružných vlnovců vyrobených z oceli. Důkladněji byla analyzována vzpěra pasivního systému VIS s označením ELVIS, jehož konstrukce se stala inspirací pro tuto práci. Jedná se o tříparametrický systém, v němž je tlumič uložen na pružině, jejíž tuhost přibližně odpovídá objemové tuhosti vlnovců respektive jejímu průmětu do axiálního směru (pressure thrust stiffness). V práci je představena metodika pro stanovení “pressure thrust stiffness” na základě geometrie vlnovce a také uvedeny parametry vlnovce díky kterým je možné měnit poměr mezi axiální a “pressure thrust stiffness” vlnovce. Tento poměr ovlivňuje v dané koncepci vzpěry její dynamické chování a tím i chování celého VIS. Pro predikci dynamického chování vzpěry byl vytvořen multi-body model VIS založeného na Stewartově plošině a detailnější model jediné vzpěry. Simulace provedené v tomto modelu odhalily parametry, které mají vliv na výkonost tlumiče ve VIS: časová odezva a dynamický rozsah. Díky modelu byl určen rozsah těchto parametrů, ve kterých bude zaručena efektivní funkce vzpěry ve VIS, konkrétně: časová odezva: 0-5ms, dynamický rozsah: 5-10. Před finálním návrhem vzpěry byla sestrojena vzpěra experimentální vzpěra, jejíž parametry byly přesně naměřeny a využity pro verifikaci jednotlivých modelů. Poznatky získané během experimentů byly využity při návrhu finální vzpěry. Jeden z nejdůležitějších poznatků byla nutnost náhrady feritového magnetického obvodu s ohledem na jeho křehkost. Proto byl odvozen tvarový přístup k navrhování rychlých magnetických obvodů z oceli s využitím 3D tisku, který byl následně patentován. Navržená vzpěra obsahuje magnetoreologický ventil jehož odezva je predikována na 1.2 ms a dynamický rozsah 10. V závěru práce je představena metodika, díky které byla vzpěra navržena.

The aim of this work was to design the construction of transport equipment under industrial robot, pallet holder or other equipment. This manipulation device can be moved in one axis on a rigid guide. The device is designed for use in a linear manufacturing system for handling workpieces and tools. It is capable of operating two rows of machines when it is placed in the middle. The main function of this device is to extend the workspace to the carried devices. This type of construction can be used in combination with an industrial robot for other technological tasks such as welding or light machining. The main parts of this device include statically mounted beds that can be stacked behind each other to form optionally long conveyor paths and a positioning plate that moves along the path formed by these beds. An industrial robot, pallet holder or other device is attached to this positioning plate. In addition to the design itself, the thesis also includes a thorough research of the issue, further possibilities of solution in the form of concepts, verification of the structure by means of calculations and simulations and implementation of the solution into a specific production cell.