No description available.

It is the purpose of this paper to set forth the method and results of studying the gamma rays resulting from the bombardment of As75 with approximately 14 Mev neutrons. The source of these neutrons was the H3(d,n)He4 reaction. The deuterons of 325 Kev energy were obtained from a Van de Graff electrostatic accelerator. A NaI scintillation spectrometer was used to determine the gamma-ray energies.

No description available.

The binding energy of the tritium nucleus is calculated by the variational method, using static repulsive core potentials. / Thesis / Master of Science (MSc)

Metastin, the product of metastasis suppressor gene KiSS-1, is proposed to be the natural ligand for the G-protein-coupled receptor GPR54, known also as AXOR12. This immunohistochemical study, using a rabbit polyclonal antiserum against the human metastin fragment (45-54)-NH2, showed that in rats metastin-like immunoreactivity (MTS-LI) was present in neurons of the nucleus of the solitary tract and caudoventrolateral reticular nucleus, and in cell processes of the spinal trigeminal tract and lateral reticular nucleus. MTS-LI was confined mainly to neurons and fibers at or caudal to the area postrema. In the spinal cord, MTS-LI cell processes formed a dense plexus in superficial layers I and II of the dorsal horn. The pattern of distribution of MTS-LI in the medulla and spinal cord suggests that this novel peptide may participate in autonomic and sensory neural signaling.

Thesis (Ph. D.)--University of Virginia, 2008. / Title from title page. Includes bibliographical references. Also available online through Digital Dissertations.

Meter a lapse of about five years the experimental attack on the secrets of the otomio nucleus hau begun again. The num her of ocientistr3 now engaged in this work and the number of particle decelerators which exact or are under coaztruction are very much greater than at any other p rcviouo time. 1ý Since the gar there have emerged several new promising methods of accelerating particles to very high enargiea. In every case the size, expense and time of eonmtruction of the o, pnratu3 are very 1nrge. The machine which is most suitable for accelerating particles to the highest energies is the proton synchrotron. Until recently this development wes confined to the BirminChe. n University, whore a machine designed to' accelerate protons to 109 eV (and possibly deuterons and of -particles to "6x 109 aV and 109 eV reapoctinely) in v2cll under construction. It id with the theory and construction of such an accelerator that this thesis is mainly concerned (Chapters III, IV and V). While building ouch a complicated picco of apparatus it in bf course essential to appreciate its likely uses. Since the proton synchrotron will be capable of deliýrering pcrtiolea over a very viide ranee of energies (protons from 2 ý- 1,000 MoV) the possible experim©nta are likely to include nearly all thoac cihich can be performed with arty accelerator. Naturally attention will be confined largely to the very high energy region.

The mesoaccumbens dopamine system has been implicated in many basic psychological processes (e.g. "wanting" and "liking") and illnesses (e.g. addiction, depression, schizophrenia). However, the precise computational functions of nucleus accumbens and dopamine neurons within the system remain unknown. In this thesis, we test some of the current hypotheses regarding the function of this system using a behavioural neurophysiology approach in the rat. The first question we wanted to answer was whether nucleus accumbens neurons process reward-predictive stimuli (e.g. conditioned reinforcers) and reward delivery differently, since previous studies report equivocal findings. To do so, we trained thirsty rats to bar-press on a second-order schedule of saccharin reinforcement, within which the temporal pattern of rats' bar-pressing was reinforced by presentations of a conditioned reinforcer and primary reinforcer (reward). We found that nucleus accumbens neurons typically responded to these conditioned and primary reinforcers with opposite sign, which suggests they were processed differently. We were not sure whether responses to conditioned reinforcers encoded reward-prediction or facilitated a behavioural switch in the rat's behaviour. Indeed, since studies using a variety of experimental techniques have implicated the mesoaccumbens dopamine system in both reward prediction and behavioural switching, we sought to test whether neurons in the nucleus accumbens and dopamine-rich areas of the midbrain respond to outcome-associated stimuli to predict reward or switch behaviour. We found both sets of neurons predominantly did the former. Finally, to understand more about reward consummatory responses from both sets of neurons, we developed a rat behavioural task providing measures of reward "wanting" and "liking". In conclusion, on the basis of our data, the most parsimonious explanation for the function of the mesoaccumbens dopamine system is that it acts to modulate goal-seeking behaviour. Further research is required to identify the function of the interactions between nucleus accumbens and dopamine neurons during goal-seeking and goal consumption.

Potato yellow dwarf virus (PYDV) is the type member of the genus Nucleorhabdovirus. The virus replicates in the nuclei of infected cells and mature virions accumulate in the perinuclear space after viral cores bud through the inner nuclear membrane. The virus was first described as an extremely destructive pathogen of potato (Solanum tuberosum) and other members of family Solanaceae. There are two different strains of PYDV based on their insect-vector specificity, namely SYDV (sanguinolenta strain) and CYDV (constricta strain). PYDV is considered a model system to study virus-vector relationship, particularly for agriculturally harmful rhabdoviruses. However, very little is known about the molecular aspects and cell biology of PYDV. Preliminary studies showed that infection of transgenic Nicotiana benthamiana plants that constitutively express GFP targeted to endomembranes with SYDV and SYNV (Sonchus yellow net virus, another member of genus Nucleorhabdovirus) results in increased accumulation of GFP and membrane within the infected nuclei, though the pattern of GFP accumulation is completely different for the two viruses. GFP accumulation was found mainly in the external and internal loci of the nucleus in SYDV-infected cells, where as, in the case of SYNV infection, the GFP accumulation was scattered throughout the nucleus of the infected cell. Molecular characterization of SYDV was undertaken to better understand the cellular difference between these two members of Nucleorhabdoviruses. This dissertation describes the determination of the complete nucleotide and ORF (open reading frame) sequences of N (nucleocapsid) and P (Phosphoprotein) gene of SYDV from cDNA clones of both viral genomic and messenger RNAs. Analyses of sequence showed that SYDV-N mRNA contains an 11 nucleotide (nt) untranslated region followed by a 1416 nt ORF encoding a 472 amino acid (aa) protein and P-mRNA contains an 18 nt 5 untranslated region followed by 840 nt ORF encoding a 280 aa protein. Characterization of SYDV-N and P protein using bioinformatic algorithms predict basic hydrophilic and coiled coil regions that may posses the putative nuclear localization signal and protein-protein interaction domain, respectively. Comparison of the SYDV-N ORF with orthologous regions from other plant and animal rhabdoviruses showed statistically significant identity. Phylogenetic analysis based on consensus N-ORFs placed SYDV into the same group with other Nucleorhabdoviruses. Localization studies of SYDV-N and P protein as autofluorescent protein fusions revealed that both proteins are exclusively nuclear localized. Taken together, this dissertation reports a detailed analysis of the biology of SYDV-N and P protein at the molecular and cellular level for the first time towards the long term goal to characterize the entire SYDV genome and to better understand SYDV-host interaction

No description available.