The purpose of this study is to investigate the efficiencies of bioremediation of sandy beach contaminated by crude oil. In this study, we used two lab-scale tanks, tank-A and tank-B, to simulate a sandy beach contaminated by crude oil. In the experiments, we added the bioreagents and oleophilic fertilizers (Inipol EAP 22®) in order to enrich crude oil degradation of sandy beach. The crude oil was degraded by nature culture in tank-A, while the crude oil was degraded by the mixed culture of several pure strains¡]MO-1, KS-1, DS-4, PF-4, PH-2, B-1, TF and XY-2¡^. We divided the experiment into five stages in this study. In conclusion, we found that the oleophilic fertilizers could increase both of the growth rate of oil degradation bacteria and crude oil biodegradation rate. However, a part of the fertilizers might flow into the ocean due to idal current. The addition of mixed culture could help to improve the biodegradation rate of sandy beach contaminated by crude oil.