E-learning within the healthcare sector is increasing in use and popularity. Implementing an online training program is especially useful in healthcare sectors where the working environment often is stressful since it allows for some flexibility. This thesis investigates how an online training program can be constructed accounting for healthcare professionals’ (HCP) needs in their use of a stroke diagnosis and rehabilitation tool, and specifically how using enactment when completing the training program affects subsequent recall after a 24-hour delay compared to not using enactment. The study consisted of two parts, a survey of current users’ experiences of a stroke diagnosis and rehabilitation tool and a quasi-experiment. The survey included 19 experienced occupational therapists and physiotherapists and revealed insights to training satisfaction, usability challenges and desired improvements. Partly based on the results from the initial survey, a subsequent online stroke training program was developed and tested in a quasi-experiment involving 16 healthcare students out of which eight enacted actions and eight did not enact during training. The online training program consisted of videos, text and knowledge and subjective experience questions. After a 24-hour delay a final questionnaire containing training contents questions was distributed. The results showed no significant difference in recall between the groups on factual knowledge of the tool. A slight difference in favor of the enactment design in one of the questions assessing participants' confidence was found. Further research on how enactment affects knowledge versus subjective experience questions with larger samples is suggested.