<p>Prostasomes are submicron-sized, membrane-bound organelles produced by the epithelial cells of the prostate and normally found in the secretion in the gland ducts. Their physiological role is in the promotion of sperm-function in human reproduction. This thesis contains four papers dealing with the production of prostasomes and some possible applications in clinical urology of the prostasome. </p><p>Paper I and II provided an ultrastructural description of the synthesis, storage and secretion of prostasomes in benign as well as in malignant tissue. Most notable were the extracellular appearances of prostasomes in metastatic lesions whereby the prostasomes become exposed to the immune system of the patient. This supported findings in earlier studies in which patients with advanced prostate cancer had elevated levels of anti-prostasome antibodies. The results of paper III reinforced the view of the prostate-unique origin of the prostasome. In particular, there were no indications in SDS-PAGE patterns or flow-cytometric studies of material from seminal vesicle secretion that it contained components that could be associated with a production of prostasomes. </p><p>Some possible clinical functions of the prostasomes were investigated in paper IV. Exposure of prostasomes to the immune system through mechanical and thermal trauma to the prostate did not induce an evident formation of anti-prostasome autoantibodies. Furthermore, the serum levels of anti-prostasome antibodies registered by assays with preparations of prostasomes from seminal plasma as antigen did not correlate with existing prostate cancer. Seminal prostasomes seemed not to function as substitute markers for prostate cancer in the test kit used. A possible explanation could be underestimated differences in antigen properties between seminal or prostate gland-derived prostasomes and prostasomes from tumor tissue.</p>

Thesis (M.Com.)(Agricultural Economics)--University of Pretoria, 2005. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.

Prostasomes are submicron-sized, membrane-bound organelles produced by the epithelial cells of the prostate and normally found in the secretion in the gland ducts. Their physiological role is in the promotion of sperm-function in human reproduction. This thesis contains four papers dealing with the production of prostasomes and some possible applications in clinical urology of the prostasome. Paper I and II provided an ultrastructural description of the synthesis, storage and secretion of prostasomes in benign as well as in malignant tissue. Most notable were the extracellular appearances of prostasomes in metastatic lesions whereby the prostasomes become exposed to the immune system of the patient. This supported findings in earlier studies in which patients with advanced prostate cancer had elevated levels of anti-prostasome antibodies. The results of paper III reinforced the view of the prostate-unique origin of the prostasome. In particular, there were no indications in SDS-PAGE patterns or flow-cytometric studies of material from seminal vesicle secretion that it contained components that could be associated with a production of prostasomes. Some possible clinical functions of the prostasomes were investigated in paper IV. Exposure of prostasomes to the immune system through mechanical and thermal trauma to the prostate did not induce an evident formation of anti-prostasome autoantibodies. Furthermore, the serum levels of anti-prostasome antibodies registered by assays with preparations of prostasomes from seminal plasma as antigen did not correlate with existing prostate cancer. Seminal prostasomes seemed not to function as substitute markers for prostate cancer in the test kit used. A possible explanation could be underestimated differences in antigen properties between seminal or prostate gland-derived prostasomes and prostasomes from tumor tissue.

Sverige är i ekonomiska sammanhang ett litet land, en begränsad hemmamarknad tvingar företag utomlands för att möjliggöra en expansion. Sverige är internationellt känt som en designnation och uttrycken ”Swedish Grace” samt ”Swedish Modern” har blivit synonymt med kvalitet, säkerhet och nytänkande. Problemet som denna uppsats ämnar att undersöka är i vilken utsträckning svenska design- företag anpassar eller standardiserar svenskheten vid en utlandsetablering. Syftet med uppsatsen är att med en jämförande studie utvärdera och analysera hur tre stycken små och ett stort svenskt designföretag väljer marknadsföringsstrategi vid en utlandsetablering. Vi har ingående studerat dessa fyra företag och datainsamlingen har skett med hjälp av kvalitativa intervjuer med representanter från företagen. Vår undersökning har visat att valet att anpassa eller standardisera svenskheten är kunskap- och erfarenhetsstyrt, men även beroende av företagets storlek. Skillnaderna vi sett mellan det stora och de mindre företagen kan följaktligen kopplas samman med ekonomiska förutsättningar samt deras erfarenhet från den nya marknaden.

Mitochondria are eukaryotic cellular organelles responsible for power-generation, believed to have come into existence by an endo-symbiontic event where a bacterial cell was incorporated by an un-specified "proto-eukaryote". Phylogenetic analysis have shown that the mitochondrial ancestor was most related to present-day alpha-proteobacteria, although the exact nature of the mitochondrial progenitor remains disputed. In this work, I have used phylogenetic and other methods to investigate the identity of the organism giving rise to mitochondria, by analysing the evolutionary history of select proteins, the events where they have been transfered to the eukaryotic nucleus, and the time-point of mitochondrial establishment. In addition, a search for mitochondrially related organisms in the ocean metagenome was performed, in the hope that something more related to the mitochondrial progenitor than anything previously identified could be found. Previous analysis have shown that a large fraction of mitochondrial proteins does indeed trace their descent to the alpha-proteobacteria, but I found that the amino-acyl tRNA-synthetases display more general bacterial descent, making it likely that these proteins are of a different origin from the mitochondria themselves. While the synthetases are encoded on the nuclear genome, most mitochondria still posses most of the tRNA on their own genomes. In the cases where the tRNA has been lost from the mitochondrial genome, I found that the probability of loss correspond to the evolutionary history of their synthetase. The ocean metagenome represents an order of magnitude more data than previously available, making it suitable for improving the analyses dealing with mitochondrial placement. This large of amount of data was utilised to improve the phylogenetic analyses, showing that previous works might have suffered from artefacts inflating the support for placement of mitochondria with a specific alpha-proteobacterial group. Eukaryotic/mitochondrial radiation was shown to be as old, or older, than radiation of extant alpha-proteobacteria, casting doubt on previous analysis identifying a specific alpha-proteobacterial group as the mitochondrial ancestor.

Research Question: What is it that determines if large companies in the same country and industry use their Country of Origin or not when they want to attract new customers abroad? Purpose: Our purpose with this Minor Field Study is to find out if companies within the Brazilian textile industry are using their COO and identify which factors that determine why they use it or not. By analyzing these factors, if and why they are important or not, we can develop a model with the purpose to give companies an idea of how close they are from to being able to use their COO. Method: We have worked with a qualitative research method where we interviewed two Brazilian companies, CI Hering and Karsten, which is working within the Brazilian textile industry. Theoretical framework: Our theories are mainly concerning the subjects of branding, nation brands, COO and competitive identity. We have also studied the so called Nation Brands Index and its hexagon. We will use theories of nation branding and COO to illustrate their importance to a country’s international companies. Empirical framework: This part will show the outcome of our interviews with Hering and Karsten but also present some data on the nation brand of Brazil and activities linked to it. Conclusion: We have found that there are six factors that mainly determine if a company will use its COO when trying to attract new customers abroad, and how appropriate this will be. The six factors are: Strong identity/image, brand awareness, knowledge, consistent and strong nation brand, research and willingness. Our final conclusions are that international companies that want to manage their reputation can benefit from relating their identity to some of the aspects in the national identity of their country. Associating to your COO is a way of doing this that aligns your company´s image to the image of your home country.

Abstract Title: Does the Country of Origin Matter for Sustainable Products? A Comparison of European Sporting Goods Producers Level: Final assignment for Master Degree in Business Administration Author: Edith Anna Voithofer Supervisor: Jonas Kågström, Ph.D. Date: January 24, 2013 Aim of study: The brand name and its country of origin is information that is used from consumers to make purchase decisions. Although existing research discusses several country of origin – aspects from other industries, literature within the sporting goods industry is still spare. Environmental pollution is an important topic nowadays, and the sporting goods industry is trying to include more “green” aspects within their business strategy, or is already quite successful in doing so. To fill the research gap, this thesis focuses on sustainable producers with a very good sustainable reputation in order to analyze the importance of the country of origin effect within the sustainable outdoor industry. Methodology: For this paper a conclusive research design was taken, as it is more formal, and used to test specific relationships. The quantitative research included respondents from two sustainable outdoor brands (originally from Sweden and Germany) who answered an online survey. Basis for the selection process was the independent bluesign® standard, a strong and global sustainability standard. The survey included five constructs to quantify the dimensions of brand equity and overall brand equity. Results: Both respondent groups are similar regarding their demographic data characteristics and their opinion when it comes to the quality of outdoor goods and its brand association. In particular, they seem to feel strongly connected towards the brands; they were proud and very loyal. Nevertheless, differences were identified. Sustainable products seem to be valued higher from Swedish respondents, whereas loyalty seems to be more important for German participants. Contribution of the study: Although existing research discusses several country of origin related aspects from other industries, relevant literature within the sporting goods industry, and here specifically the sustainable outdoor industry is still sparse. These topics are covered in this study. Keywords: Country of origin, Sustainability, Sporting Goods Industry, Outdoor Industry

Cell cycle exit (“birth”) of retinal progenitor cells (RPCs) is considered a watershed that is preceded by changing levels of cell cycle regulators, and followed rapidly by induction of a post M-phase differentiation cascade. Yet the actual dynamics of these events are largely unclear, thus whether mitosis separates pre- and post- birth differentiation cascades is unproven. We characterized the regulation of many division and differentiation markers relative to each other and final mitosis. Unexpectedly, classic “cell cycle” markers were present well beyond exit (e.g. Ki67, Pcna), early embryonic RPCs expressed “differentiation” markers that later labeled post-mitotic neurons exclusively (e.g. Brn3b, Tubb3, Ptf1a), and factors detected just after cell birth in the embryo were induced well beyond M-phase post-natally (e.g. Nrl, Crx). Thus, the dynamics of birth-associated events shift dramatically during development, even to either side of mitosis. Instead of mitosis behaving as a cog that activates post-exit differentation events we suggest that a common trigger induces both the exit and differentiation programs in RPCs, precisely coordinating their startpoints, but that each subsequent cascade unfolds independently. This model explains the convergence of birth and differentiation but also their temporal maliability. This view fits with our observation that in the absence of the Rb tumor suppressor, differentiation still initiates even without cell cycle exit. Finally, neoplastic transformation in the mouse retina requires loss of Rb and its relative p107, and emerging tumor features suggest an amacrine cell-of-origin. We studied Rb/p107 null clones, and noted two striking features. First, despite initial expansion of aberrantly dividing differentiating cells, apoptosis pruned clones precisely to wild type sizes. “Cell competition” maintains tissue size by selecting fitter over weaker progenitors; our data provide a unique example of competition among differentiating cells. Second, despite normal numbers of amacrine cells per Rb/p107 null clone, more clones contained amacrine cells and fewer had bipolar cells. Both this effect and ectopic division were E2f1-dependent. Thus, the oncogenic initiation event in mouse retinoblastoma triggers a very early fate switch, even before neoplastic transformation, broadening the possibilities for the cell-of-origin of retinoblastoma, and arguing that even very early stage tumors cannot be used to define cancer origin.

Cell cycle exit (“birth”) of retinal progenitor cells (RPCs) is considered a watershed that is preceded by changing levels of cell cycle regulators, and followed rapidly by induction of a post M-phase differentiation cascade. Yet the actual dynamics of these events are largely unclear, thus whether mitosis separates pre- and post- birth differentiation cascades is unproven. We characterized the regulation of many division and differentiation markers relative to each other and final mitosis. Unexpectedly, classic “cell cycle” markers were present well beyond exit (e.g. Ki67, Pcna), early embryonic RPCs expressed “differentiation” markers that later labeled post-mitotic neurons exclusively (e.g. Brn3b, Tubb3, Ptf1a), and factors detected just after cell birth in the embryo were induced well beyond M-phase post-natally (e.g. Nrl, Crx). Thus, the dynamics of birth-associated events shift dramatically during development, even to either side of mitosis. Instead of mitosis behaving as a cog that activates post-exit differentation events we suggest that a common trigger induces both the exit and differentiation programs in RPCs, precisely coordinating their startpoints, but that each subsequent cascade unfolds independently. This model explains the convergence of birth and differentiation but also their temporal maliability. This view fits with our observation that in the absence of the Rb tumor suppressor, differentiation still initiates even without cell cycle exit. Finally, neoplastic transformation in the mouse retina requires loss of Rb and its relative p107, and emerging tumor features suggest an amacrine cell-of-origin. We studied Rb/p107 null clones, and noted two striking features. First, despite initial expansion of aberrantly dividing differentiating cells, apoptosis pruned clones precisely to wild type sizes. “Cell competition” maintains tissue size by selecting fitter over weaker progenitors; our data provide a unique example of competition among differentiating cells. Second, despite normal numbers of amacrine cells per Rb/p107 null clone, more clones contained amacrine cells and fewer had bipolar cells. Both this effect and ectopic division were E2f1-dependent. Thus, the oncogenic initiation event in mouse retinoblastoma triggers a very early fate switch, even before neoplastic transformation, broadening the possibilities for the cell-of-origin of retinoblastoma, and arguing that even very early stage tumors cannot be used to define cancer origin.

As companies become ever more globalised, manufacture firms choose to outsource production to lower labour cost countries. However, as studies have shown, such a relocation of production may lead to undesirably decreased quality perceptions by consumers as the brand origin and country of production are de-coupled. This quality perception linked to congruity between brand origin and country of production has been studied for various products and product classes, but little has been written about how a luxury bed manufacturer may be affected by this phenomenon known as the country of origin effect. Thus in theory quality perceptions are higher if the brand origin and country of production are congruent and are further amplified if that country is already regarded highly in terms of production competence. In our study, we set out to verify this theory through a consumer-based questionnaire. Our results give strong evidence in support of this theory which is why we ultimately claim, from a theoretical standpoint, that the company we focus on should maintain its production location in Sweden.