A movimentação dentária ortodôntica ocorre através de dois processos, nos quais o osso alveolar é reabsorvido nas áreas de pressão, enquanto que novo osso é formado na área de tração. O processo de reabsorção óssea ocorre pela ação de células multinucleadas, os osteoclastos. Os bisfosfonatos constituem um grupo de fármacos com propriedade de inibir a reabsorção óssea, foi utilizado no presente estudo com a finalidade de interferir na remodelação óssea induzida ortodonticamente. Para isso, força contínua de 15 cN foi aplicada aos primeiros molares superiores de ratos machos Wistar de 2 1/2 meses, utilizando uma biomecânica com fios superelásticos. Os animais foram divididos aleatoriamente em 4 grupos: 1) O grupo controle constituído por dezoito ratos, os quais foram injetados solução salina por 7 dias antes da instalação da biomecânica passiva, que permaneceu por 3, 10 e 18 dias; 2) Dezoito animais foram tratados com ALN (dose 2,5 mg/Kg) por 7 dias antes da instalação da biomecânica passiva que permaneceu por 3, 10 e 18 dias; 3) Dezoito animais foram tratados com alendronato com a mesma dose citada acima por 7 dias antes da instalação da biomecânica ativa que permaneceu por 3, 10 e 18 dias; 4) Dezoito animais foram injetados com solução salina 7 dias antes da instalação da biomecânica ativa que permaneceu por 3, 10 e 18 dias. As maxilas foram fixadas com 4% de formaldeído + 0,1% de glutaraldeído, descalcificadas em EDTA a 4,13% e incluídas em parafina ou resina Spurr. Os cortes foram corados com HE para análise morfológica. Alguns cortes foram submetidos à imuno-histoquímica para detecção de RANKL e OPG. Foi utilizado o método TRAP, marcador de osteoclastos e microscopia eletrônica de transmissão para análise ultraestrutural. Alguns espécimes tiveram a cortical óssea vestibular do primeiro molar superior congelada em nitrogênio líquido para análise da expressão de RANKL por Western Blotting. O ALN inibiu a reabsorção óssea e radicular de todos os grupos tratados. As células clásticas apresentaram-se em estado latente. No grupo da movimentação ortodôntica o osso alveolar foi remodelado e com 18 dias a superfície radicular apresentou-se reabsorvida e o TRAP revelou clastos ativos, achados confirmados pela microscopia eletrônica de transmissão. A expressão de RANKL, molécula ativadora de células clásticas, nao foi inibida pela droga. A expressão de OPG foi aumentada nos animais tratados. Os resultados demonstram que o uso de alendronato sódico na movimentação ortodôntica não interfere no recrutamento dos osteoclasto, ele aparentemente inibe sua ativação, o que pode interferir no processo de remodelação óssea e talvez diminua a quantidade de movimentação dentária. / Orthodontic tooth movement occurs through two processes in which the alveolar bone is resorbed in the pressure areas, whereas new bone is formed in the tension area. The bone resorption occurs by multinucleated cell, the osteoclasts. The bisphosphonates are drugs with capability to inhibit clastic activity were used in the present study in order to interfere with the bone remodeling induced orthodontic. For this continuous force of 15 cN was applied to the first molars of Wistar male rats of 2 1/2 months, using a biomechanical with superelastic wire. The animals were randomly divided into 4 groups: 1) The control group consisted of eighteen mice, which received sterile saline solution saline for 7 days prior to installation of passive biomechanics, which remained for 3, 10 and 18 days; 2) Eighteen animals were treated with ALN (dose 2.5 mg / kg) for 7 days prior to installation of the passive biomechanical to remain for 3, 10 and 18 days; 3) Eighteen animals were treated with alendronate with the same dose quoted above for 7 days prior to the biomechanical installation that remains active for 3, 10 and 18 days; 4) Eighteen animals were injected with sterile saline solution 7 days prior to the biomechanical installation that remains active for 3, 10 and 18 days. The maxillae were fixed with 4% formaldehyde + 0.1% glutaraldehyde, decalcified in EDTA 4.13% and embedded in paraffin or Spurr resin. The specimens were morphologically analyzed in HE stained sections. Some stained sections were used for immunolabeling for RANKL and OPG. The osteoclasts were marked by tartrate-resistant acid phosphatase (TRAP) histochemistry. The ultrathin sections were examined in a trasnmission electron micrsocpe. Some specimens were frozen in liquid nitrogen for protein extraction and Western Blotting protein expression analyzes. The ALN inhibited bone resorption and root of all the treated groups. The clastic cells present in a latent state. In the orthodontic movement group alveolar bone was remodeled with 18 days to root surface presented itself reabsorbed and the TRAP revealed clasts assets, findings confirmed by transmission electron microscopy. Expression of RANKL activating molecule clastic cells was not inhibited by the drug. The OPG expression was increased in treated animals. The results demonstrate that the use of alendronate in the orthodontic movement does not interfere with osteoclast recruitment, it apparently inhibits their activation, which can interfere in the bone remodeling process and may reduce the amount of tooth movement.

Alterações celulares e neurovasculares na polpa têm sido relacionadas a forças ortodónticas, usadas na movimentação dentária. As alterações observadas são geralmente relacionadas a alterações vasculares na polpa. Os primeiros estudos sobre alterações do fluxo sangüíneo pulpar (FSP) durante a movimentação dentária foram realizados usando técnicas qualitativas (e. g., cortes histológicos), e quantitativas (e. g., micro-esferas radioativas ou fluorescentes, radioespirometria). Tais técnicas são invasivas, destrutivas e não permitem o estudo de alterações dinâmicas do fluxo. Recentemente a fluxometria laser Doppler (FLD), uma técnica não invasiva, tem sido usada no estudo de alterações dinâmicas do FSP em humanos em condições próximas às reais. Mas os estudos disponíveis na literatura, usando a FLD, ainda são limitados a movimentos experimentais, pois ainda existem limitações práticas na FLD. O objetivo desta pesquisa foi, usando a FLD, estudar alterações do FSP de voluntários submetidos a duas fases de um tratamento ortodôntico corrente (nivelamento e retração), dentro de condições clinicas reais. Doze voluntários foram monitorados durante a fase de nivelamento e treze voluntários foram monitorados durante a fase de retração, antes da aplicação de forças, 20 minutos, 48h, 72h e 1 mês depois de aplicadas as forças. Analisando os resultados obtidos, durante as duas fases estudas foram encontradas evidências estatísticas suficientes para inferir que ocorrem alterações significativas no FSP, quando medidas via FLD, durante os movimentos e forças estudadas, nos seguintes momentos investigados: diminuição do FSP nos momentos 20 minutos, 48h e 72h. Durante o nivelamento, no dia 30 o FSP é próximo ou igual ao basal. Durante a retração, no dia 30 o fluxo ainda permanece diminuído. / Cellular and neurovascular changes in the pulp have been related to orthodontic forces, used to produce a dental movement. The observed alterations are generally related to pulpal vascular changes. Earlier studies on pulpal blood flow (PBF) changes during the dental movement were carried out by using qualitative techniques (e. g., histological slices), and quantitative (e. g., radioactive or fluorescent microspheres, radiospirometry). Such techniques are invasive, destructive, and do not allow dynamic flow changes to be studied. Recently the laser Doppler flowmetry (LDF), a non-invasive technique, has been used to study dynamic PBF alterations in humans into nearly real conditions. The available studies in the literature, by using the LDF, are limited to experimental studies, probably due to the LDF practical limitations. The aim of this work was, by using the FDF, to study PBF alterations from volunteers subjected to two phases of a current orthodontic treatment (leveling and retraction), into real clinical conditions. Twelve volunteers were monitored during the leveling phase and thirteen volunteers were monitored during the retraction phase, before the force to be applied, and 20 minutes, 48 hr, 72 hr and 1 month later. Analyzing the obtained results, during the two studied phases, it were found suffice statistical evidences to infer that significant PBF decrease occurs on the investigates moments: 20 minutes, 48 hr and 72 hr. During the leveling phase, on day 30, the PBF is near or equal to the basal level. During the retraction, on day 30, the flow is still decreased.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O objetivo do estudo foi mensurar as alterações na quantidade do volume de fluido gengival (FG) dos dentes submetidos à movimentação ortodôntica, em adultos com periodontite crônica na fase de manutenção periodontal. O FG foi coletado com uma tira de papel absorvente padrão das áreas de pressão dos dentes de 10 pacientes (sete homens e três mulheres) e medido em microlitro no Periotron em seis dias distintos (dia -7, dia 0, dia 1, dia 7, dia 14 e dia 21). Os pacientes foram submetidos a um rigoroso programa de controle de placa. O teste de Wilcoxon foi aplicado para comparar os dados obtidos. Os resultados mostraram que o controle de placa efetivo baixou a quantidade de FG do dia -7 para o dia 0, e em seguida houve uma elevação do FG até o dia 7 por conta do início da movimentação ortodôntica, e uma nova queda do dia 7 até o 21. O maior volume de FG foi encontrado no dia 7, uma semana após o início da movimentação. O menor volume no dia 0. Concluindo, a movimentação ortodôntica aumentou o volume de FG do dia 0 até o dia 7. / The aim of this study was to measure the alterations in the amount of gingival crevicular fluid (GCF) of teeth during orthodontic tooth movement in adults with chronic periodontitis under a periodontal maintenance program. The GCF was collected with paper stripes from pression sides of teeth in 10 patients (7 males and 3 females) and measured in microlitres on Periotron in six distinct periods of time (day -7, day 0, day 1, day 7, day 14, day 21). Patients were under supervised program of plaque control. Wilcoxon test was applied to compare the data. The results shown that the effective plaque control reduces the amount of GCF from day -7 to day 0, followed by an elevation until day 7 after the beginning of orthodontic tooth movement and a new reduce from day 7 to 21. The highest amount of GCF was found on day 7, one week after the beginning of tooth movement, while the smallest found on day 0.

Biological responses to orthodontic forces involve various cell types, these include fibroblasts, endothelial cells, blood vessels and sensory nerves in the periodontal ligament as well as osteoblasts, osteoclasts and cementoblasts in roots and bone surfaces. Neurotrophins are believed to interact with these cells to initiate the process of bone resorption particularly during orthodontic tooth movement. Neuropeptides released from sensory neurons have been shown to modulate the tissue inflammatory responses. In addition, neurotrophins, including nerve growth factor (NGF), play an important role in neural cell differentiation and survival. The exact localization and function of neurotrophins and neurotrophic receptors in the dento-alveolar complex remains unclear. Moreover, the identity and distribution of structures expressing neurotrophins and neurotrophic receptors has yet to be fully determined. It is reasonable to propose that periodontal ligament and alveolar bone remodelling may be influenced by NGF. In addition, anti-NGF may block neurochemical changes and, hence, inhibit orthodontic tooth movement. The aims of this research were to investigate the cells responsible for NGF secretion within the periodontal ligament (PDL), pulp and bone, and the effect that anti-NGF might have on orthodontic tooth movement. 28, 8 week-old, male Sprague-Dawley rats were randomly divided into control and experimental groups. Fourteen experimental animals had anti-NGF injected paradentally. Animals were sacrificed at 7 and 14 days. Sections from an earlier study were examined and stained using TRAP for osteoclast identification and analysed histomorphometrically to enable comparisons between control and experimental groups. The findings of this investigation indicated that injections of anti-NGF did not significantly affect the rate of tooth movement with the use of different tooth movement measurement methods. TRAP staining proved to be a useful and reliable marker of osteoclasts. TRAP-positive osteoclastic cells were detected in both anti-NGF and control groups. However, the TRAP-positive cells were not stained intensely with NGF immunolabelling. On the other hand, cells that were stained intensely with NGF, were TRAP-negative. The results suggested that both sympathetic and nociceptive nerves might function in counter balance to modulate bone resorption, and osteoclasts might not be directly responsible for NGF secretion within the PDL and bone. Further studies to determine the effect of NGF on tooth movement are warranted to more clearly identify the NGF expressing cells within the rat dento-alveolar complex and possible role played by NGF in orthodontic tooth movement. / http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297498 / Thesis (D.Clin.Dent.)-- School of Dentistry, 2007

Biological responses to orthodontic forces involve various cell types, these include fibroblasts, endothelial cells, blood vessels and sensory nerves in the periodontal ligament as well as osteoblasts, osteoclasts and cementoblasts in roots and bone surfaces. Neurotrophins are believed to interact with these cells to initiate the process of bone resorption particularly during orthodontic tooth movement. Neuropeptides released from sensory neurons have been shown to modulate the tissue inflammatory responses. In addition, neurotrophins, including nerve growth factor (NGF), play an important role in neural cell differentiation and survival. The exact localization and function of neurotrophins and neurotrophic receptors in the dento-alveolar complex remains unclear. Moreover, the identity and distribution of structures expressing neurotrophins and neurotrophic receptors has yet to be fully determined. It is reasonable to propose that periodontal ligament and alveolar bone remodelling may be influenced by NGF. In addition, anti-NGF may block neurochemical changes and, hence, inhibit orthodontic tooth movement. The aims of this research were to investigate the cells responsible for NGF secretion within the periodontal ligament (PDL), pulp and bone, and the effect that anti-NGF might have on orthodontic tooth movement. 28, 8 week-old, male Sprague-Dawley rats were randomly divided into control and experimental groups. Fourteen experimental animals had anti-NGF injected paradentally. Animals were sacrificed at 7 and 14 days. Sections from an earlier study were examined and stained using TRAP for osteoclast identification and analysed histomorphometrically to enable comparisons between control and experimental groups. The findings of this investigation indicated that injections of anti-NGF did not significantly affect the rate of tooth movement with the use of different tooth movement measurement methods. TRAP staining proved to be a useful and reliable marker of osteoclasts. TRAP-positive osteoclastic cells were detected in both anti-NGF and control groups. However, the TRAP-positive cells were not stained intensely with NGF immunolabelling. On the other hand, cells that were stained intensely with NGF, were TRAP-negative. The results suggested that both sympathetic and nociceptive nerves might function in counter balance to modulate bone resorption, and osteoclasts might not be directly responsible for NGF secretion within the PDL and bone. Further studies to determine the effect of NGF on tooth movement are warranted to more clearly identify the NGF expressing cells within the rat dento-alveolar complex and possible role played by NGF in orthodontic tooth movement. / http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297498 / Thesis (D.Clin.Dent.)-- School of Dentistry, 2007

Submitted by Juliana da Costa Lisbôa (julianalisboa86@hotmail.com) on 2018-09-10T11:26:11Z No. of bitstreams: 1 Juliana C Lisboa.pdf: 1704367 bytes, checksum: 0a852a606d663babe8ff1cd313f64b57 (MD5) / Approved for entry into archive by Silvana Alvarez null (silvana@ict.unesp.br) on 2018-09-10T19:21:58Z (GMT) No. of bitstreams: 1 lisboa_jc_me_sjc.pdf: 1704367 bytes, checksum: 0a852a606d663babe8ff1cd313f64b57 (MD5) / Made available in DSpace on 2018-09-10T19:21:59Z (GMT). No. of bitstreams: 1 lisboa_jc_me_sjc.pdf: 1704367 bytes, checksum: 0a852a606d663babe8ff1cd313f64b57 (MD5) Previous issue date: 2018-07-13 / A perda do primeiro molar é uma situação comum na rotina do consultório e com isso surgem alternativas de tratamento para reabilitação, sendo uma delas, o fechamento de espaço através da mesialização do segundo molar. O uso de mini- implante proporciona ancoragem máxima, costuma ser bem tolerado pelo paciente e se destaca pela facilidade de instalação e remoção, apresentando dessa maneira desempenho clínico bastante favorável. O objetivo desse estudo foi analisar a distribuição de tensões em modelo que simula a mesialização de molar inferior utilizando diferentes tipos de mini-implantes. Embasado no método dos elementos finitos foi obtido modelo ósseo de mandíbula por recomposição de uma tomografia computadorizada realizada pelo software Invesalius (CTI, São Paulo, Brasil). O desenho do segundo molar foi obtido por técnica de recomposição tomográfica. Elementos de ancoragem auto-perfurantes da marca Neodent e também auto-rosqueantes da marca Titanium Fix foram desenhados e modelados por ferramenta de revolução do perfil desenhado no software Rhinoceros 3D, inseridos ao bloco ósseo e então submetidos a cargas de 200 cN. Os resultados foram calculados e analisados pelo programa Ansys 17.0 para verificação por meio da análise de mapas de deslocamento e tensões máximas principais, principalmente do osso cortical e indicaram que, dentro do modelo experimental, o ligamento periodontal apresentou valores baixos de tensão máxima principal, porém dentro dos valores fisiológicos para prover a movimentação, com visualização de áreas de compressão e de tração com escala entre 0,1 a -0,1 MPa. Concluiu-se que a carga testada permite a deslocamento dentário porém com tendência a giroversão. / The loss of the first molar is a common situation in the routine of the dentist and with this treatment alternatives for rehabilitation arises, one of them being the closure of space through the mesialization of the second molar. The use of mini-implants provides maximum anchorage, is well tolerated by the patient and stands out for ease installation and removal, thus presenting a very favorable clinical performance. The objective of this study was to analyze the stress distribution in a model that simulates the inferior molar mesialization using different types of mini- implants. Based on the finite element method, a mandible bone model was obtained by recomposing a computerized tomography performed by invesalius software (CTI, São Paulo, Brazil). The second molar design was obtained by a technique similar to the tomographic recomposition. Neodent self-piercing anchoring elements and also Titanium Fix self-piercing anchors were designed and modeled by a revolution tool in the Rhinoceros 3D software, inserted into the bone block and then subjected to loads of up to 200 cN. The results were calculated and analyzed by the Ansys 17.0 program for verification by means of the analysis of displacement maps and main maximum stresses, mainly of the cortical bone and indicated that, within the experimental model, the periodontal ligament presented low values of main maximum tension, however within the physiological values to provide the movement, with visualization of areas of compression and traction with scale between 0.1 and -0.1 MPa. It was concluded that the load tested allows dental displacement but with a tendency to gyroversion.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O objetivo do estudo foi mensurar as alterações na quantidade do volume de fluido gengival (FG) dos dentes submetidos à movimentação ortodôntica, em adultos com periodontite crônica na fase de manutenção periodontal. O FG foi coletado com uma tira de papel absorvente padrão das áreas de pressão dos dentes de 10 pacientes (sete homens e três mulheres) e medido em microlitro no Periotron em seis dias distintos (dia -7, dia 0, dia 1, dia 7, dia 14 e dia 21). Os pacientes foram submetidos a um rigoroso programa de controle de placa. O teste de Wilcoxon foi aplicado para comparar os dados obtidos. Os resultados mostraram que o controle de placa efetivo baixou a quantidade de FG do dia -7 para o dia 0, e em seguida houve uma elevação do FG até o dia 7 por conta do início da movimentação ortodôntica, e uma nova queda do dia 7 até o 21. O maior volume de FG foi encontrado no dia 7, uma semana após o início da movimentação. O menor volume no dia 0. Concluindo, a movimentação ortodôntica aumentou o volume de FG do dia 0 até o dia 7. / The aim of this study was to measure the alterations in the amount of gingival crevicular fluid (GCF) of teeth during orthodontic tooth movement in adults with chronic periodontitis under a periodontal maintenance program. The GCF was collected with paper stripes from pression sides of teeth in 10 patients (7 males and 3 females) and measured in microlitres on Periotron in six distinct periods of time (day -7, day 0, day 1, day 7, day 14, day 21). Patients were under supervised program of plaque control. Wilcoxon test was applied to compare the data. The results shown that the effective plaque control reduces the amount of GCF from day -7 to day 0, followed by an elevation until day 7 after the beginning of orthodontic tooth movement and a new reduce from day 7 to 21. The highest amount of GCF was found on day 7, one week after the beginning of tooth movement, while the smallest found on day 0.

Alterações celulares e neurovasculares na polpa têm sido relacionadas a forças ortodónticas, usadas na movimentação dentária. As alterações observadas são geralmente relacionadas a alterações vasculares na polpa. Os primeiros estudos sobre alterações do fluxo sangüíneo pulpar (FSP) durante a movimentação dentária foram realizados usando técnicas qualitativas (e. g., cortes histológicos), e quantitativas (e. g., micro-esferas radioativas ou fluorescentes, radioespirometria). Tais técnicas são invasivas, destrutivas e não permitem o estudo de alterações dinâmicas do fluxo. Recentemente a fluxometria laser Doppler (FLD), uma técnica não invasiva, tem sido usada no estudo de alterações dinâmicas do FSP em humanos em condições próximas às reais. Mas os estudos disponíveis na literatura, usando a FLD, ainda são limitados a movimentos experimentais, pois ainda existem limitações práticas na FLD. O objetivo desta pesquisa foi, usando a FLD, estudar alterações do FSP de voluntários submetidos a duas fases de um tratamento ortodôntico corrente (nivelamento e retração), dentro de condições clinicas reais. Doze voluntários foram monitorados durante a fase de nivelamento e treze voluntários foram monitorados durante a fase de retração, antes da aplicação de forças, 20 minutos, 48h, 72h e 1 mês depois de aplicadas as forças. Analisando os resultados obtidos, durante as duas fases estudas foram encontradas evidências estatísticas suficientes para inferir que ocorrem alterações significativas no FSP, quando medidas via FLD, durante os movimentos e forças estudadas, nos seguintes momentos investigados: diminuição do FSP nos momentos 20 minutos, 48h e 72h. Durante o nivelamento, no dia 30 o FSP é próximo ou igual ao basal. Durante a retração, no dia 30 o fluxo ainda permanece diminuído. / Cellular and neurovascular changes in the pulp have been related to orthodontic forces, used to produce a dental movement. The observed alterations are generally related to pulpal vascular changes. Earlier studies on pulpal blood flow (PBF) changes during the dental movement were carried out by using qualitative techniques (e. g., histological slices), and quantitative (e. g., radioactive or fluorescent microspheres, radiospirometry). Such techniques are invasive, destructive, and do not allow dynamic flow changes to be studied. Recently the laser Doppler flowmetry (LDF), a non-invasive technique, has been used to study dynamic PBF alterations in humans into nearly real conditions. The available studies in the literature, by using the LDF, are limited to experimental studies, probably due to the LDF practical limitations. The aim of this work was, by using the FDF, to study PBF alterations from volunteers subjected to two phases of a current orthodontic treatment (leveling and retraction), into real clinical conditions. Twelve volunteers were monitored during the leveling phase and thirteen volunteers were monitored during the retraction phase, before the force to be applied, and 20 minutes, 48 hr, 72 hr and 1 month later. Analyzing the obtained results, during the two studied phases, it were found suffice statistical evidences to infer that significant PBF decrease occurs on the investigates moments: 20 minutes, 48 hr and 72 hr. During the leveling phase, on day 30, the PBF is near or equal to the basal level. During the retraction, on day 30, the flow is still decreased.

A movimentação dentária ortodôntica ocorre através de dois processos, nos quais o osso alveolar é reabsorvido nas áreas de pressão, enquanto que novo osso é formado na área de tração. O processo de reabsorção óssea ocorre pela ação de células multinucleadas, os osteoclastos. Os bisfosfonatos constituem um grupo de fármacos com propriedade de inibir a reabsorção óssea, foi utilizado no presente estudo com a finalidade de interferir na remodelação óssea induzida ortodonticamente. Para isso, força contínua de 15 cN foi aplicada aos primeiros molares superiores de ratos machos Wistar de 2 1/2 meses, utilizando uma biomecânica com fios superelásticos. Os animais foram divididos aleatoriamente em 4 grupos: 1) O grupo controle constituído por dezoito ratos, os quais foram injetados solução salina por 7 dias antes da instalação da biomecânica passiva, que permaneceu por 3, 10 e 18 dias; 2) Dezoito animais foram tratados com ALN (dose 2,5 mg/Kg) por 7 dias antes da instalação da biomecânica passiva que permaneceu por 3, 10 e 18 dias; 3) Dezoito animais foram tratados com alendronato com a mesma dose citada acima por 7 dias antes da instalação da biomecânica ativa que permaneceu por 3, 10 e 18 dias; 4) Dezoito animais foram injetados com solução salina 7 dias antes da instalação da biomecânica ativa que permaneceu por 3, 10 e 18 dias. As maxilas foram fixadas com 4% de formaldeído + 0,1% de glutaraldeído, descalcificadas em EDTA a 4,13% e incluídas em parafina ou resina Spurr. Os cortes foram corados com HE para análise morfológica. Alguns cortes foram submetidos à imuno-histoquímica para detecção de RANKL e OPG. Foi utilizado o método TRAP, marcador de osteoclastos e microscopia eletrônica de transmissão para análise ultraestrutural. Alguns espécimes tiveram a cortical óssea vestibular do primeiro molar superior congelada em nitrogênio líquido para análise da expressão de RANKL por Western Blotting. O ALN inibiu a reabsorção óssea e radicular de todos os grupos tratados. As células clásticas apresentaram-se em estado latente. No grupo da movimentação ortodôntica o osso alveolar foi remodelado e com 18 dias a superfície radicular apresentou-se reabsorvida e o TRAP revelou clastos ativos, achados confirmados pela microscopia eletrônica de transmissão. A expressão de RANKL, molécula ativadora de células clásticas, nao foi inibida pela droga. A expressão de OPG foi aumentada nos animais tratados. Os resultados demonstram que o uso de alendronato sódico na movimentação ortodôntica não interfere no recrutamento dos osteoclasto, ele aparentemente inibe sua ativação, o que pode interferir no processo de remodelação óssea e talvez diminua a quantidade de movimentação dentária. / Orthodontic tooth movement occurs through two processes in which the alveolar bone is resorbed in the pressure areas, whereas new bone is formed in the tension area. The bone resorption occurs by multinucleated cell, the osteoclasts. The bisphosphonates are drugs with capability to inhibit clastic activity were used in the present study in order to interfere with the bone remodeling induced orthodontic. For this continuous force of 15 cN was applied to the first molars of Wistar male rats of 2 1/2 months, using a biomechanical with superelastic wire. The animals were randomly divided into 4 groups: 1) The control group consisted of eighteen mice, which received sterile saline solution saline for 7 days prior to installation of passive biomechanics, which remained for 3, 10 and 18 days; 2) Eighteen animals were treated with ALN (dose 2.5 mg / kg) for 7 days prior to installation of the passive biomechanical to remain for 3, 10 and 18 days; 3) Eighteen animals were treated with alendronate with the same dose quoted above for 7 days prior to the biomechanical installation that remains active for 3, 10 and 18 days; 4) Eighteen animals were injected with sterile saline solution 7 days prior to the biomechanical installation that remains active for 3, 10 and 18 days. The maxillae were fixed with 4% formaldehyde + 0.1% glutaraldehyde, decalcified in EDTA 4.13% and embedded in paraffin or Spurr resin. The specimens were morphologically analyzed in HE stained sections. Some stained sections were used for immunolabeling for RANKL and OPG. The osteoclasts were marked by tartrate-resistant acid phosphatase (TRAP) histochemistry. The ultrathin sections were examined in a trasnmission electron micrsocpe. Some specimens were frozen in liquid nitrogen for protein extraction and Western Blotting protein expression analyzes. The ALN inhibited bone resorption and root of all the treated groups. The clastic cells present in a latent state. In the orthodontic movement group alveolar bone was remodeled with 18 days to root surface presented itself reabsorbed and the TRAP revealed clasts assets, findings confirmed by transmission electron microscopy. Expression of RANKL activating molecule clastic cells was not inhibited by the drug. The OPG expression was increased in treated animals. The results demonstrate that the use of alendronate in the orthodontic movement does not interfere with osteoclast recruitment, it apparently inhibits their activation, which can interfere in the bone remodeling process and may reduce the amount of tooth movement.

La métalloprotéinase matricielle (MMP)-8 ou collagénase-2 est capable de rompre les molécules natives, triples hélices, du collagène interstitiel, initiant ainsi le remodelage cellulaire lors du déplacement dentaire induit par une force orthodontique. C'est un bio-marqueur incontournable du remaniement tissulaire parodontal. L'augmentation de l'expression et de l'activation de MMP-8 dans le fluide gingival reflète l'activité du remodelage parodontal induit par les forces orthodontiques. En moyenne, la concentration de MMP-8 prélevée dans le fluide gingival des patients orthodontiques est 12 fois plus élevée (56 ± 50 µg/l contre 4,6 ± 4 µg/l) que chez les patients non orthodontiques. Le suivi des fluctuations de MMP-8 durant le déplacement orthodontique nécessite la mise au point d'un biocapteur. L'objectif de ce travail est d'utiliser une structure photonique à base de silicium poreux pour la conception d'un biocapteur optique de la MMP-8. Nous avons déterminé le choix du substrat de silicium poreux (PSi) le plus adapté à notre application avec une surface spécifique élevée et pores suffisamment ouverts pour l'infiltration des biomolécules qui sont des anticorps anti-MMP-8. Ensuite nous avons mis en place un procédé de fonctionnalisation chimique et biologique de la surface interne de ces échantillons. / The matrix métalloprotéinase (MMP) 8 or collagenase 2 is able to cleave native molecules, triple helixes, of the interstitial collagen, so introducing the cellular reshaping during orthodontic tooth movement. It is a major biomarker of the periodontal tissular remodeling. The increase of the expression and the activation of MMP-8 in the gingival fluid reflects the activity of the periodontal remodeling. On average, the concentration of MMP-8 taken in the gingival fluid of the orthodontic patients is 12 times as raised(brought up) (56 ± 50 µg / l against 4,6 ± 4 µg / l) that at the not orthodontic patients. Followed it by fluctuations in MMP-8 during the orthodontic movement require the development of a biosensor. The objective of this work is to use a photonique structure with porous silicon for the conception of an optical biosensor of the MMP-8. We determined the choice of the substratum of porous silicon (PSi) the most adapted to our application with a high specific surface and pores opened enough for the infiltration of the biomolecules which are antibodies anti-MMP-8. Then we set up a process of chemical and biological fonctionnalisation of the internal surface of these samples.