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OSMOTIC AND METABOLIC RESPONSES TO DEHYDRATION AND UREA-LOADING IN A TERRESTRIALLY-HIBERNATING FROGMuir, Timothy J. 28 June 2007 (has links)
No description available.
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Acute and chronic adaptation of Supraoptic neurons to changes in osmolalityMumtaz, Naima 20 June 2011
Vasopressin (VP) is an antidiuretic hormone that is synthesized and released by osmosensitive magnocellular neurosecretory cells (MNCs) to regulate water homeostasis in the body. The rate and firing pattern of MNCs determines the amount of VP release, which is elevated during physiological stress particularly dehydration. During acute osmotic changes the MNCs shrink and swell due to hypertonic and hypotonic stimuli, respectively. In contrast to hippocampal neurons, which display regulatory volume increases (RVI) and regulatory volume decreases (RVD) in response to hypertonic and hypotonic stimuli, MNCs do not have compensatory mechanisms. The MNCs undergo hypertrophy as a part of their physiological structural and functional plasticity during chronic dehydration. These changes are thought to be important during long term osmotic changes for the sustained and high level releases of hormone. However, the mechanism of hypertrophy is still unclear and it is difficult to address this issue in vivo. We therefore undertook studies on acutely isolated MNCs to test hypertrophy in MNCs. We observed that acutely isolated MNCs treated with hyperosmolar solution (325 mOsmol kg-1) for 150 minutes in vitro showed hypertrophy (a 9% increase in CSA) and recovered their original size when returned to isotonic solution (295 mOsmol kg-1) for another 60 minutes. Whole cell patch clamp experiments showed a 34% increase in cell membrane capacitance following treatment with hypertonic solution for 90-150 minutes. The osmotically-evoked hypertrophic response was blocked by using a TAT (human immunodeficiency virus transactivator of transcription) peptide (TAT-NSF700) that prevents SNARE-mediated exocytotic fusion by blocking the function of NSF (N-ethylmaleimide-sensitive factor). The hypertrophic response did not appear to be altered by a scrambled version of the peptide, showing that osmotically-evoked hypertrophy depends on SNARE-mediated exocytotic fusion. The VP and OT-MNCs exposed to hyperosmolar solution for two hours showed increased immunofluorescence for L-type Ca²⁺ channels (both Cav1.2 and Cav1.3). Our data suggest that the osmotically-evoked hypertrophy is associated with an increase in the total membrane surface area due to the exocytotic fusion of intracellular granules with the plasma membrane and with increased expression of L-type Ca2+ channels. This study will be helpful in understanding of the adaptation that MNCs undergo during long term dehydration and pathological conditions that lead to increased plasma osmolality.
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Acute and chronic adaptation of Supraoptic neurons to changes in osmolalityMumtaz, Naima 20 June 2011 (has links)
Vasopressin (VP) is an antidiuretic hormone that is synthesized and released by osmosensitive magnocellular neurosecretory cells (MNCs) to regulate water homeostasis in the body. The rate and firing pattern of MNCs determines the amount of VP release, which is elevated during physiological stress particularly dehydration. During acute osmotic changes the MNCs shrink and swell due to hypertonic and hypotonic stimuli, respectively. In contrast to hippocampal neurons, which display regulatory volume increases (RVI) and regulatory volume decreases (RVD) in response to hypertonic and hypotonic stimuli, MNCs do not have compensatory mechanisms. The MNCs undergo hypertrophy as a part of their physiological structural and functional plasticity during chronic dehydration. These changes are thought to be important during long term osmotic changes for the sustained and high level releases of hormone. However, the mechanism of hypertrophy is still unclear and it is difficult to address this issue in vivo. We therefore undertook studies on acutely isolated MNCs to test hypertrophy in MNCs. We observed that acutely isolated MNCs treated with hyperosmolar solution (325 mOsmol kg-1) for 150 minutes in vitro showed hypertrophy (a 9% increase in CSA) and recovered their original size when returned to isotonic solution (295 mOsmol kg-1) for another 60 minutes. Whole cell patch clamp experiments showed a 34% increase in cell membrane capacitance following treatment with hypertonic solution for 90-150 minutes. The osmotically-evoked hypertrophic response was blocked by using a TAT (human immunodeficiency virus transactivator of transcription) peptide (TAT-NSF700) that prevents SNARE-mediated exocytotic fusion by blocking the function of NSF (N-ethylmaleimide-sensitive factor). The hypertrophic response did not appear to be altered by a scrambled version of the peptide, showing that osmotically-evoked hypertrophy depends on SNARE-mediated exocytotic fusion. The VP and OT-MNCs exposed to hyperosmolar solution for two hours showed increased immunofluorescence for L-type Ca²⁺ channels (both Cav1.2 and Cav1.3). Our data suggest that the osmotically-evoked hypertrophy is associated with an increase in the total membrane surface area due to the exocytotic fusion of intracellular granules with the plasma membrane and with increased expression of L-type Ca2+ channels. This study will be helpful in understanding of the adaptation that MNCs undergo during long term dehydration and pathological conditions that lead to increased plasma osmolality.
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Hypoxia-induced Decrease in Renal Medullary Osmolality: Prevention with dDAVPVoicu, Laura 16 February 2010 (has links)
Acute kidney injury (AKI) may result from perioperative renal medullary hypoxia. Despite high oxygen delivery to the kidney, the medullary thick ascending limb (mTAL) in the outer renal medulla is susceptible to hypoxia because of its high oxygen consumption and relatively low rate of blood flow. The objective of this study was to evaluate the effect of a low pO2 (8% FiO2 for 5 h) on the major function of the mTAL and to develop a strategy to protect the mTAL in this setting. Evidence of hypoxia-induced reduction in mTAL function included low interstitial and urine osmolality but only a minimal rise in Na+ excretion; this was prevented by pre-treatment with desmopressin acetate (dDAVP), a vasopressin analogue which may increase tissue pO2. A decrease in urine osmolality may be of diagnostic value for hypoxic renal damage and dDAVP may prevent acute kidney injury in the perioperative setting.
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Hypoxia-induced Decrease in Renal Medullary Osmolality: Prevention with dDAVPVoicu, Laura 16 February 2010 (has links)
Acute kidney injury (AKI) may result from perioperative renal medullary hypoxia. Despite high oxygen delivery to the kidney, the medullary thick ascending limb (mTAL) in the outer renal medulla is susceptible to hypoxia because of its high oxygen consumption and relatively low rate of blood flow. The objective of this study was to evaluate the effect of a low pO2 (8% FiO2 for 5 h) on the major function of the mTAL and to develop a strategy to protect the mTAL in this setting. Evidence of hypoxia-induced reduction in mTAL function included low interstitial and urine osmolality but only a minimal rise in Na+ excretion; this was prevented by pre-treatment with desmopressin acetate (dDAVP), a vasopressin analogue which may increase tissue pO2. A decrease in urine osmolality may be of diagnostic value for hypoxic renal damage and dDAVP may prevent acute kidney injury in the perioperative setting.
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The Investigation of Tear Film Osmolality as a Clinical Instrument Used in Assessments of the Tear Film and Dry Eye DiseaseDalton, Kristine Nicole January 2009 (has links)
Introduction:
Tear film osmolality is a product of the varying concentrations of dissolved solutes (proteins, lipids and mucins) in the tear fluid. Research suggests that a hyperosmotic tear film is a trait common to all
forms of dry eye, and it may be the driving force causing the discomfort, ocular surface damage and inflammation found in both evaporative and tear deficient forms of dry eye disease. Tear film osmolality has been proposed to be the “gold standard” diagnostic test for the evaluation of dry eye
disease, as a distinct separation between tear film osmolalities in normal and dry-eyed (aqueous deficient or evaporative) populations has become evident.
Historically, tear film osmolality could only be measured in a laboratory setting and required a highly skilled technician to use the instrumentation. The recent development of easy-to-use, small volume osmometers has made it possible for tear film osmolality to be measured clinically. As these instruments are quite new, there has been very little research completed with them. Therefore, a
series of studies was conducted to investigate the utility of one of these new osmometers – the Advanced Instruments Model 3100 Nanolitre Osmometer.
The specific aims of each chapter were:
- Chapter 3: To determine if the Advanced Instruments Model 3100 Nanolitre Osmometer was capable of quantitatively measuring tear film osmolality in a normal population, using 0.5μL tear samples.
- Chapter 4: Previous studies have shown the Advanced Instruments Model 3100 Nanolitre
Osmometer not significantly different from another commercially available osmometer
(Wescor Vapor Pressure Osmometer) for the measurement of human tears. This chapter
examined the repeatability of the new instrument over multiple measurements on the same sample and over multiple days.
- Chapter 5: To determine if tear film osmolality values varied significantly over the course of a normal working day in a population that was primarily free from symptoms of dry eye.
- Chapter 6: To investigate the relationships between tear film osmolality and other commonly used clinical tests for dry eye disease. The clinical tests examined included various questionnaires designed to assess patient symptoms (Single Item Dry Eye Questionnaire (SIDEQ), the Ocular Surface Disease Index (OSDI), and the McMonnies Dry Eye Questionnaire (MMDEQ) and a linear analogue comfort scale (LACS)), a non-invasive tear break-up time test (NIBUT), and examination of ocular surface redness and tear ferning (TF). Secondarily to determine if the other clinical tests demonstrated significant diurnal variations
over the course of a normal working day.
- Chapter 7: To measure tear film osmolality in a population with mild to moderate symptoms of dry eye disease, and to compare this value with the osmolality of a population of age-matched controls without the disease. Secondarily, to investigate the relationship between tear film osmolality and patient comfort in a population with mild to moderate symptoms of dry eye disease.
Methods:
- Chapter 3: Tears were collected from 40 volunteer participants with a capillary tube. Some participants were non-contact lens wearers (Non-CL), while others wore either soft or rigid contact lenses (CL). Tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 4: Tears were collected from 10 volunteer participants using two different collection techniques. Collections were repeated on three separate days (6 study visits total); three osmolality measurements per collection were taken using the Advanced Instruments Model 3100 Nanolitre osmometer.
- Chapter 5: Tears were collected from 40 volunteer participants in two separate studies (n=80 in total). Tears were collected with a capillary tube three times a day (morning, mid-day and afternoon), on two separate days (6 study visits total). Tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 6: Clinical tests were administered and tear samples were collected using a capillary tube from 40 volunteer participants. Measurements were taken three times a day (morning, mid-day and afternoon), on two separate days (6 study visits total). Tear film osmolality was
measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 7: Participants were classified as either having dry eye disease (DE) or not having dry eye disease (NDE) based on a clinical examination that included a case history, phenol red thread test and biomicroscopy (white light and sodium fluorescein assessment). Tear samples were then collected from all participants using a capillary tube and tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer. Participants also completed the SIDEQ, the OSDI, and the MMDEQ.
Results:
- Chapter 3: The mean tear film osmolality of the population was 298.7±11.4mOsm/Kg. CL
wear (soft or rigid) did not appear to have a significant effect on tear film osmolality (CL: 298.5±11.2mOsm/Kg vs. Non-CL: 298.9±11.5mOsm/Kg), although this study was not designed to specifically look at the effects of contact lens wear on tear film osmolality.
- Chapter 4: There was reasonably good concordance between measurements of tear film
osmolality taken with the Advanced Instruments Model 3100 Nanolitre Osmometer
(intraclass correlations range from 0.6497 (F= 0.0582) to 0.9550 (F = 0.5893)). Repeatability appeared to be affected by significant changes in ambient humidity (>10% per day). Concordance was similar with both sampling techniques.
- Chapter 5: In the first study, no significant diurnal change in tear film osmolality was found (p>0.05), although a significant difference in measurements taken on Day 1 compared to Day 2 was found (p=0.040). When the first and last 10 participants enrolled were compared, the
difference between days was present in the first 10 participants, but not in the last 10; it is likely that the investigator underwent a learning process during the period of the study, and that reflex tearing occurred more often in the early portion of the study compared with the latter portion. In the second study, no significant diurnal change in tear film osmolality was found (p>0.05) and no significant difference in measurements taken on Day 1 compared to Day 2 was found (p>0.05). When tear film osmolality was compared with the number of
hours participants were awake, no significant correlation was found (r = 0.07044).
- Chapter 6: Significant correlations were not found between tear film osmolality and SIDEQ (r = 0.1347), OSDI (r = 0.0331), MMDEQ (r = 0.2727), LACS (r = -0.1622), NIBUT (r = -0.2280), subjectively graded redness (r=-0.2280), or objectively measured redness (r = 0.1233). A weakly significant correlation was found between TF and tear film osmolality (r = 0.3978). None of
the clinical measures (LACS, NIBUT, subjective or objective redness or TF) varied
significantly over the course of the day.
- Chapter 7: Tear film osmolality was higher in both the right (DE = 311.1±12.4mOsm/Kg, NDE = 306.2±11.2mOsm/Kg) and left eyes (DE = 313.2±11.9mOsm/Kg, NDE =
304.0±7.5mOsm/Kg) of participants, but the difference was only statistically significant in the left eye. Tear film osmolality did not correlate significantly with DE patient symptoms using any of the questionnaires (SIDEQ, OSDI, MMDEQ).
Conclusions:
- Chapter 3: The Advanced Instruments Model 3100 Nanolitre Osmometer appeared to be
capable of measuring tear film osmolality in a normal population. Our population mean was
slightly lower than what is reported to be normal (305mOsm/Kg), but it still fell within the range of values reported as normal (297 – 318mOsm/Kg).
- Chapter 4: The Advanced Instruments Model 3100 Nanolitre Osmometer demonstrated
reasonably good repeatability for the measurement of human tear samples. Unfortunately, the instrumentation appeared to be affected by dramatic weather changes. Maintaining the instrument in a humidity controlled environment may resolve this problem.
- Chapter 5: Tear film osmolality did not appear to vary significantly over a normal working day. Inducing reflex tearing, perhaps with an unskilled investigator collecting the tears, can be a significant source of error (as demonstrated in the first study).
- Chapter 6: Tear film osmolality did not correlate well with other clinical instruments designed to assess either patient symptoms or signs of dry eye disease in a normal population. Tear film osmolality and tear ferning did demonstrate a weakly significant positive correlation. None of the clinical measures assessed demonstrated a significant diurnal variation over the course of a normal working day.
- Chapter 7: Tear film osmolality appeared to be higher in participants with mild to moderate symptoms of dry eye when compared with age matched, asymptomatic controls. Tear film osmolality did not correlate well with patient symptoms in a population of mild to moderate severe dry eyed individuals.
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The Investigation of Tear Film Osmolality as a Clinical Instrument Used in Assessments of the Tear Film and Dry Eye DiseaseDalton, Kristine Nicole January 2009 (has links)
Introduction:
Tear film osmolality is a product of the varying concentrations of dissolved solutes (proteins, lipids and mucins) in the tear fluid. Research suggests that a hyperosmotic tear film is a trait common to all
forms of dry eye, and it may be the driving force causing the discomfort, ocular surface damage and inflammation found in both evaporative and tear deficient forms of dry eye disease. Tear film osmolality has been proposed to be the “gold standard” diagnostic test for the evaluation of dry eye
disease, as a distinct separation between tear film osmolalities in normal and dry-eyed (aqueous deficient or evaporative) populations has become evident.
Historically, tear film osmolality could only be measured in a laboratory setting and required a highly skilled technician to use the instrumentation. The recent development of easy-to-use, small volume osmometers has made it possible for tear film osmolality to be measured clinically. As these instruments are quite new, there has been very little research completed with them. Therefore, a
series of studies was conducted to investigate the utility of one of these new osmometers – the Advanced Instruments Model 3100 Nanolitre Osmometer.
The specific aims of each chapter were:
- Chapter 3: To determine if the Advanced Instruments Model 3100 Nanolitre Osmometer was capable of quantitatively measuring tear film osmolality in a normal population, using 0.5μL tear samples.
- Chapter 4: Previous studies have shown the Advanced Instruments Model 3100 Nanolitre
Osmometer not significantly different from another commercially available osmometer
(Wescor Vapor Pressure Osmometer) for the measurement of human tears. This chapter
examined the repeatability of the new instrument over multiple measurements on the same sample and over multiple days.
- Chapter 5: To determine if tear film osmolality values varied significantly over the course of a normal working day in a population that was primarily free from symptoms of dry eye.
- Chapter 6: To investigate the relationships between tear film osmolality and other commonly used clinical tests for dry eye disease. The clinical tests examined included various questionnaires designed to assess patient symptoms (Single Item Dry Eye Questionnaire (SIDEQ), the Ocular Surface Disease Index (OSDI), and the McMonnies Dry Eye Questionnaire (MMDEQ) and a linear analogue comfort scale (LACS)), a non-invasive tear break-up time test (NIBUT), and examination of ocular surface redness and tear ferning (TF). Secondarily to determine if the other clinical tests demonstrated significant diurnal variations
over the course of a normal working day.
- Chapter 7: To measure tear film osmolality in a population with mild to moderate symptoms of dry eye disease, and to compare this value with the osmolality of a population of age-matched controls without the disease. Secondarily, to investigate the relationship between tear film osmolality and patient comfort in a population with mild to moderate symptoms of dry eye disease.
Methods:
- Chapter 3: Tears were collected from 40 volunteer participants with a capillary tube. Some participants were non-contact lens wearers (Non-CL), while others wore either soft or rigid contact lenses (CL). Tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 4: Tears were collected from 10 volunteer participants using two different collection techniques. Collections were repeated on three separate days (6 study visits total); three osmolality measurements per collection were taken using the Advanced Instruments Model 3100 Nanolitre osmometer.
- Chapter 5: Tears were collected from 40 volunteer participants in two separate studies (n=80 in total). Tears were collected with a capillary tube three times a day (morning, mid-day and afternoon), on two separate days (6 study visits total). Tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 6: Clinical tests were administered and tear samples were collected using a capillary tube from 40 volunteer participants. Measurements were taken three times a day (morning, mid-day and afternoon), on two separate days (6 study visits total). Tear film osmolality was
measured with the Advanced Instruments Model 3100 Nanolitre Osmometer.
- Chapter 7: Participants were classified as either having dry eye disease (DE) or not having dry eye disease (NDE) based on a clinical examination that included a case history, phenol red thread test and biomicroscopy (white light and sodium fluorescein assessment). Tear samples were then collected from all participants using a capillary tube and tear film osmolality was measured with the Advanced Instruments Model 3100 Nanolitre Osmometer. Participants also completed the SIDEQ, the OSDI, and the MMDEQ.
Results:
- Chapter 3: The mean tear film osmolality of the population was 298.7±11.4mOsm/Kg. CL
wear (soft or rigid) did not appear to have a significant effect on tear film osmolality (CL: 298.5±11.2mOsm/Kg vs. Non-CL: 298.9±11.5mOsm/Kg), although this study was not designed to specifically look at the effects of contact lens wear on tear film osmolality.
- Chapter 4: There was reasonably good concordance between measurements of tear film
osmolality taken with the Advanced Instruments Model 3100 Nanolitre Osmometer
(intraclass correlations range from 0.6497 (F= 0.0582) to 0.9550 (F = 0.5893)). Repeatability appeared to be affected by significant changes in ambient humidity (>10% per day). Concordance was similar with both sampling techniques.
- Chapter 5: In the first study, no significant diurnal change in tear film osmolality was found (p>0.05), although a significant difference in measurements taken on Day 1 compared to Day 2 was found (p=0.040). When the first and last 10 participants enrolled were compared, the
difference between days was present in the first 10 participants, but not in the last 10; it is likely that the investigator underwent a learning process during the period of the study, and that reflex tearing occurred more often in the early portion of the study compared with the latter portion. In the second study, no significant diurnal change in tear film osmolality was found (p>0.05) and no significant difference in measurements taken on Day 1 compared to Day 2 was found (p>0.05). When tear film osmolality was compared with the number of
hours participants were awake, no significant correlation was found (r = 0.07044).
- Chapter 6: Significant correlations were not found between tear film osmolality and SIDEQ (r = 0.1347), OSDI (r = 0.0331), MMDEQ (r = 0.2727), LACS (r = -0.1622), NIBUT (r = -0.2280), subjectively graded redness (r=-0.2280), or objectively measured redness (r = 0.1233). A weakly significant correlation was found between TF and tear film osmolality (r = 0.3978). None of
the clinical measures (LACS, NIBUT, subjective or objective redness or TF) varied
significantly over the course of the day.
- Chapter 7: Tear film osmolality was higher in both the right (DE = 311.1±12.4mOsm/Kg, NDE = 306.2±11.2mOsm/Kg) and left eyes (DE = 313.2±11.9mOsm/Kg, NDE =
304.0±7.5mOsm/Kg) of participants, but the difference was only statistically significant in the left eye. Tear film osmolality did not correlate significantly with DE patient symptoms using any of the questionnaires (SIDEQ, OSDI, MMDEQ).
Conclusions:
- Chapter 3: The Advanced Instruments Model 3100 Nanolitre Osmometer appeared to be
capable of measuring tear film osmolality in a normal population. Our population mean was
slightly lower than what is reported to be normal (305mOsm/Kg), but it still fell within the range of values reported as normal (297 – 318mOsm/Kg).
- Chapter 4: The Advanced Instruments Model 3100 Nanolitre Osmometer demonstrated
reasonably good repeatability for the measurement of human tear samples. Unfortunately, the instrumentation appeared to be affected by dramatic weather changes. Maintaining the instrument in a humidity controlled environment may resolve this problem.
- Chapter 5: Tear film osmolality did not appear to vary significantly over a normal working day. Inducing reflex tearing, perhaps with an unskilled investigator collecting the tears, can be a significant source of error (as demonstrated in the first study).
- Chapter 6: Tear film osmolality did not correlate well with other clinical instruments designed to assess either patient symptoms or signs of dry eye disease in a normal population. Tear film osmolality and tear ferning did demonstrate a weakly significant positive correlation. None of the clinical measures assessed demonstrated a significant diurnal variation over the course of a normal working day.
- Chapter 7: Tear film osmolality appeared to be higher in participants with mild to moderate symptoms of dry eye when compared with age matched, asymptomatic controls. Tear film osmolality did not correlate well with patient symptoms in a population of mild to moderate severe dry eyed individuals.
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The Influence of Sex on the Osmoreceptor Modulation of Heat Loss ResponsesBarrera Ramirez, Juliana A 20 December 2013 (has links)
Females exhibit lower sudomotor response than males, which has been attributed to physiological differences between sexes. It is well accepted that non-thermal factors (i.e. baroreceptors and osmoreceptors) can influence thermoeffector responses. Even though there are sex-related differences in baroreceptor modulation of thermoeffector responses, it remains unknown if differences in osmoreceptor modulation could explain the lower sudomotor response in females. Therefore, we examined if there are sex-related differences in osmoreceptor modulation of sweating and cutaneous vascular conductance (CVC). A group of nine males and nine females were passively heated while in an isosmotic and hyperosmotic state. The onset and thermosensitivity of sweating and CVC were calculated and compared between groups and conditions. We show that the delay in onset of sweating and CVC is similar between sexes. However, thermosensitivity of sweating was lower in females than males. We conclude that hyperosmolality does not modulate the decreased sudomotor activity in females.
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The Influence of Sex on the Osmoreceptor Modulation of Heat Loss ResponsesBarrera Ramirez, Juliana A January 2014 (has links)
Females exhibit lower sudomotor response than males, which has been attributed to physiological differences between sexes. It is well accepted that non-thermal factors (i.e. baroreceptors and osmoreceptors) can influence thermoeffector responses. Even though there are sex-related differences in baroreceptor modulation of thermoeffector responses, it remains unknown if differences in osmoreceptor modulation could explain the lower sudomotor response in females. Therefore, we examined if there are sex-related differences in osmoreceptor modulation of sweating and cutaneous vascular conductance (CVC). A group of nine males and nine females were passively heated while in an isosmotic and hyperosmotic state. The onset and thermosensitivity of sweating and CVC were calculated and compared between groups and conditions. We show that the delay in onset of sweating and CVC is similar between sexes. However, thermosensitivity of sweating was lower in females than males. We conclude that hyperosmolality does not modulate the decreased sudomotor activity in females.
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The effects of acute handling stress on the secondary stress responses of striped bass (Morone saxtilis) and its hybrid (Morone chrysops x Morone saxtilis)Reubush, Kimberly J. 07 April 2009 (has links)
The importance of understanding the stress response can not be underestimated in fisheries research or the aquaculture industry. Three studies were undertaken to quantify the secondary stress responses of striped bass and its hybrid. These were measured as fluctuations in glucose, glycogen, lactate, and osmolality. Fish were stressed by aerial emersion in a dipnet. The first study was conducted with fingerling inland and anadromous striped bass. The three goals were to: determine if fingerlings responded with the General Adaptation Syndrome, if the two had different responses to the stress, and if feeding state (fed up until the day of the stress vs. starved for three days prior) had an effect on the stress response. The second study was conducted with two-year-old pure and hybrid striped bass. The two goals were to: determine any differences in the stress response, and to see if the feeding state played a role in the response. The third study was conducted with hybrid fingerlings. This study looked at the ability of fed and three-day starved fish to moderate their secondary stress responses after handling stress, when placed in 0, 5, 10, or 15% saline recovery water. / Master of Science
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