Lo Ting Sze. / Thesis submitted in: December 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 145-170). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.vii / List of Contents / Contents in Brief --- p.viii / Contents in Detail --- p.ix / List of Figures and Tables / List of Figures --- p.xiii / List of Tables --- p.xvi / Chapter Chapter 1 --- Introduction and literature review General introduction --- p.1 / Chapter 1.1 --- Structural changes in ovary of penaeid shrimp during maturation --- p.3 / Chapter 1.2 --- Biochemical and physiological changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1 --- Variation of protein content --- p.7 / Chapter 1.2.1.1 --- Changes of proteins in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1.2 --- Biochemical characterization of vitellin --- p.8 / Chapter 1.2.1.3 --- Site of vitellogenin synthesis --- p.10 / Chapter 1.2.2 --- Variation of lipid composition --- p.12 / Chapter 1.2.2.1 --- Role of lipids in ovary of penaeid shrimp --- p.12 / Chapter 1.2.2.2 --- Changes of lipids in ovary of penaeid shrimp during maturation --- p.13 / Chapter 1.2.3 --- Variation of other nutrient contents --- p.16 / Chapter 1.3 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.16 / Chapter 1.3.1 --- Gonad-inhibiting hormone (GIH) --- p.17 / Chapter 1.3.2 --- Crustacean hyperglycemic hormone (CHH) --- p.19 / Chapter 1.3.3 --- X-organ sinus gland complex (XOSG) and CHH neuropeptide family --- p.20 / Chapter 1.3.4 --- Gonad-stimulating hormone (GSH) --- p.22 / Chapter 1.3.5 --- Methyl farnesoate (MF) --- p.23 / Chapter 1.3.6 --- Neurotransmitters --- p.24 / Chapter 1.3.7 --- Other factors --- p.26 / Chapter 1.3.8 --- Androgenic hormone (AH) --- p.27 / Chapter 1.4 --- Ecology and reproductive biology of Metapenaeus ensis --- p.29 / Chapter 1.4.1 --- Identification --- p.29 / Chapter 1.4.2 --- Ecology --- p.29 / Chapter 1.4.3 --- Reproductive biology --- p.30 / Chapter 1.5 --- Objective of research --- p.32 / Chapter Chapter 2 --- "Variation of circulating gonad-inhibiting hormone (GIH) level during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 2.1 --- Introduction --- p.34 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- Experimental animals and serum extraction --- p.35 / Chapter 2.2.2 --- Protein content assay --- p.36 / Chapter 2.2.3 --- SDS-polyacrymide gel electrophoresis (SDS-PAGE) --- p.39 / Chapter 2.2.4 --- Western blot --- p.40 / Chapter 2.2.5 --- Purification of IgG from antiserum --- p.42 / Chapter 2.2.6 --- ELISA assay (indirect ELISA) --- p.42 / Chapter 2.3 --- Results --- p.44 / Chapter 2.3.1 --- Experimental animals --- p.44 / Chapter 2.3.2 --- Purification of anti-GIH IgG --- p.46 / Chapter 2.3.3 --- Optimization of indirect ELISA --- p.46 / Chapter 2.3.4 --- Determination of circulating GIH in haemolymph --- p.51 / Chapter 2.4 --- Discussion --- p.55 / Chapter Chapter 3 --- "Identification of genes differentially expressed during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and methods --- p.61 / Chapter 3.2.1 --- Outline of methodology --- p.61 / Chapter 3.2.2 --- Experimental animals --- p.62 / Chapter 3.2.3 --- Total RNA extraction --- p.62 / Chapter 3.2.4 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.64 / Chapter 3.2.5 --- DNA electrophoresis by agarose gel --- p.68 / Chapter 3.2.6 --- DNA electrophoresis by polyacrylamide gel --- p.68 / Chapter 3.2.7 --- Preparation of DIG-labeled probe from PCR product --- p.69 / Chapter 3.2.8 --- Checking for DIG-labeling yield --- p.69 / Chapter 3.2.9 --- Excision of cDNA library from ovary of Metapenaeus ensis --- p.70 / Chapter 3.2.10 --- PCR screening of insertion sequence --- p.73 / Chapter 3.2.11 --- Dot-blot --- p.75 / Chapter 3.2.12 --- Dot-blot hybridization --- p.76 / Chapter 3.2.13 --- Growth of cell for plasmid preparation --- p.79 / Chapter 3.2.14 --- Permanent culture preparation --- p.80 / Chapter 3.2.15 --- DNA Sequencing --- p.80 / Chapter 3.2.16 --- RNA formaldehyde denaturing gel electrophoresis --- p.82 / Chapter 3.2.17 --- Northern blot --- p.84 / Chapter 3.2.18 --- Northern hybridization --- p.84 / Chapter 3.2.19 --- 5´ة RACE (rapid amplification of cDNA ends) --- p.88 / Chapter 3.3 --- Results --- p.92 / Chapter 3.3.1 --- Experimental animals --- p.92 / Chapter 3.3.2 --- Total RNA extraction --- p.92 / Chapter 3.3.3 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.92 / Chapter 3.3.4 --- Titer of cDNA library from Metapenaeus ensis ovary --- p.95 / Chapter 3.3.5 --- PCR screening of insertion sequence --- p.95 / Chapter 3.3.6 --- Dot-blot hybridization --- p.99 / Chapter 3.3.7 --- DNA sequencing of differentially expressed genes --- p.96 / Chapter 3.3.8 --- Northern analysis --- p.99 / Chapter 3.3.8.1 --- Housekeeping gene - elongation factor 1α --- p.106 / Chapter 3.3.8.2 --- Translationally controlled tumor protein (TCTP) --- p.106 / Chapter 3.3.8.3 --- Cytoskeletal actin --- p.109 / Chapter 3.3.8.4 --- Keratin --- p.109 / Chapter 3.3.8.5 --- Heat shock cognate 70 kD protein (hsc70) --- p.109 / Chapter 3.3.8.6 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.117 / Chapter 3.3.8.7 --- Arginine kinase --- p.117 / Chapter 3.3.8.8 --- High mobility group 1-like protein (HMGl-like protein) --- p.122 / Chapter 3.3.8.9 --- Nucleoside diphosphate kinase --- p.122 / Chapter 3.4 --- Discussion --- p.122 / Chapter 3.4.1 --- RAP-PCR and dot-blot hybridization --- p.122 / Chapter 3.4.2 --- Functions of differentially expressed genes in ovarian maturation --- p.128 / Chapter 3.4.2.1 --- Translationally controlled tumor protein (TCTP) --- p.128 / Chapter 3.4.2.2 --- Cytoskeletal actin --- p.130 / Chapter 3.4.2.3 --- Keratin --- p.130 / Chapter 3.4.2.4 --- Heat shock cognate 70 kD protein (hsc70) --- p.131 / Chapter 3.4.2.5 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.134 / Chapter 3.4.2.6 --- Arginine kinase --- p.135 / Chapter 3.4.2.7 --- High mobility group 1-like protein (HMGl-like protein) --- p.136 / Chapter 3.4.2.8 --- Nucleoside diphosphate kinase --- p.137 / Chapter 3.4.3 --- Speculation on the molecular changes in ooctyes during ovarian maturation --- p.139 / Chapter Chapter 4 --- General conclusion --- p.142 / References --- p.145

Cui, Ju. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 110-136). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables and Figures --- p.xi / List of Abbreviations --- p.xiii / Chapter 1 General introduction --- p.1 / Chapter 2 Literature review --- p.4 / Chapter 2.1 --- Introduction --- p.4 / Chapter 2.2 --- Structural changes in ovary and thelycum of penaeid shrimp during maturaiton --- p.4 / Chapter 2.3 --- Biochemical changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 2.4 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.9 / Chapter 2.4.1 --- Peptides --- p.9 / Chapter 2.4.2 --- Steroids --- p.17 / Chapter 2.4.3 --- Terpenoids --- p.19 / Chapter 2.4.4 --- Biogenic amines --- p.21 / Chapter 2.6 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.23 / Chapter 2.6 --- Proteomics --- p.27 / Chapter Chapter 3 --- Identification of proteins differentially expressed during ovarian maturation in the shrimp Metapenaeus ensis --- p.28 / Chapter 3.1 --- Introduction --- p.28 / Chapter 3.2 --- Materials and methods --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Histological observation --- p.30 / Chapter 3.2.3 --- Data analysis --- p.31 / Chapter 3.2.4 --- Two-dimensional gel electrophoresis --- p.31 / Chapter 3.2.5 --- Protein isolation and identification by MALDI-TOF MS/MS --- p.35 / Chapter 3.3 --- Results --- p.37 / Chapter 3.3.1 --- General ovarian histology --- p.37 / Chapter 3.3.2 --- Morphometric analysis --- p.41 / Chapter 3.3.3 --- Comparison of proteomic patterns of shrimp ovaries and image analysis --- p.43 / Chapter 3.3.4 --- Identification of differentially expressed proteins in shrimp ovaries by MALDI-TOF MS/MS analysis --- p.48 / Chapter 3.4 --- Discussion --- p.61 / Chapter 3.4.1 --- Identification of proteins by MALDI-TOF MS/MS analysis --- p.61 / Chapter 3.4.2 --- Potential functions of the identified ditterentially expressed proteins in shrimp reproduction --- p.62 / Chapter Chapter 4 --- Characterization of cellular retinoic acid binding protein (CRABP) and retinoic X receptor (RXR) in the shrimp Metapenaeus ensis --- p.69 / Chapter 4.1 --- Introduction --- p.69 / Chapter 4.2 --- Materials and methods --- p.72 / Chapter 4.2.1 --- Experimental animals --- p.72 / Chapter 4.2.2 --- Preparation of total RNA --- p.72 / Chapter 4.2.3 --- Rapid Amplification of 5' and 3' cDNA Ends (RACE) --- p.73 / Chapter 4.2.4 --- Subcloning --- p.75 / Chapter 4.2.5 --- Sequencing --- p.78 / Chapter 4.2.6 --- Phylogenetic analysis --- p.78 / Chapter 4.2.7 --- Reverse transcription (RT) --- p.79 / Chapter 4.2.8 --- Semi-quantitative RT-PCR --- p.79 / Chapter 4.2.9 --- Real-Time RT-PCR --- p.80 / Chapter 4.2.10 --- In situ hybridization --- p.81 / Chapter 4.2.11 --- Tissue culture and in vitro ovary explant assay --- p.83 / Chapter 4.2.12 --- Statistical analysis --- p.84 / Chapter 4.3 --- Results --- p.84 / Chapter 4.3.1 --- Full-length RXR cDNA derivation --- p.84 / Chapter 4.3.2 --- Sequence comparisons and phylogenetic analyses --- p.86 / Chapter 4.3.3 --- Spatiotemporal expression profiles of MeCRABP and MeRXR mRNA in female shrimp --- p.92 / Chapter 4.3.4 --- Effect of exogenous retinoic acids on the expression of MeCRABP and MeRXR in shrimp ovaries --- p.96 / Chapter 4.4 --- Discussion --- p.101 / Chapter 4.4.1 --- Cloning and characterization of the M. ensis RXR --- p.101 / Chapter 4.4.2 --- Developmental expression of CRABP and RXR in shrimp ovary --- p.102 / Chapter 4.4.3 --- Effects of exogenous retinoic acids --- p.104 / Chapter Chapter 5 --- General conclusion --- p.106 / References --- p.110

Typescript (photocopy) / ix, 309 leaves : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (M.D.)--Dept. of Obstetrics and Gynaecology, University of Adelaide, 1981

Thesis (Ph. D.)--University of Hong Kong, 2006. / Title proper from title frame. Also available in printed format.

Thesis (M.S.)--University of Pretoria, 2005. / Title from PDF t.p. (viewed on Apr. 14, 2007). Includes bibliographical references.

Thesis (Ph. D.)--University of Hong Kong, 2006. / Also available online.

Thesis (M. Med. Sc.)--University of Hong Kong, 2009. / Includes bibliographical references (p. 52-57).

Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 61-66).

Thesis (Ph. D.)--University of Texas at Austin, 2003. / Vita. Includes bibliographical references. Available from UMI Company.

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences