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Pedilanthus palmeri Millsp. was evaluated as a potential interior landscaping plant. The optimal propagation technique, production and post-production light requirements, growth regulator response and photoperiod requirement were analyzed. Cuttings were successfully rooted within five weeks after treatment with aqueous solutions of IBA. Root fresh weight increased from 0.58 g with 0 mg·liter⁻¹ IBA to 1.12 g with 6736 mg·liter⁻¹ IBA. No difference in leaf length, width or thickness, plant height or node number was found between plants grown under 77% and 88% shade. Plants moved to a low light interior environment continued to grow and new, thin leaves replaced abscised leaves. The optimum concentration of the growth retardant Bonzi (paclobutrazol), 0.125 g ai per pot, caused a 46% reduction in shoot height. Apical pinching and 500 mg·liter-1 BA increased branch number 3.8-fold. Pedilanthus palmeri is a short day plant. Flowering occurred in the 8 and 10 hour light treatments but not in the longer photoperiods.

Glyphosate resistant crops were rapidly adopted by farmers since their introduction in 1996 and currently, greater than 90% of cotton and soybean crops are glyphosate resistant. Glyphosate has been an effective mean for controlling Palmer amaranth, however overreliance on glyphosate based systems resulted in weeds that can no longer be controlled with glyphosate. Palmer amaranth resistance to glyphosate has been confirmed in ten US states including Virginia's bordering neighbor North Carolina. The objectives of this study were to i) determine the spread of Palmer amaranth and evaluate awareness among farmers and agribusinesses of herbicide resistant weeds in Virginia; ii) determine the efficacy of commonly used cotton and soybean herbicides programs for Palmer amaranth control; and iii) conduct greenhouse experiments to quantify the level of glyphosate resistance in a Greensville County, Virginia population. Using a communication network of Virginia county extension agents and crop advisers, Palmer amaranth was found in 15 Virginia counties. A survey was conducted to evaluate awareness of herbicide resistance and management of weeds in Virginia. Ninety percent of producers had fields planted to Roundup Ready® crops for each of the last 3 years. One hundred percent of the responders claimed awareness of the potential for weeds to develop resistance to glyphosate, but when asked about how serious they consider weed resistance to herbicides, the responders average rating was of 7.9 (on a scale of 1 to 10 where 1 is "not at all serious" and 10 is "very serious" ). Eighteen percent of the responder population claimed no awareness of glyphosate resistant weeds documented in Virginia. Herbicide efficacy experiments were established in soybean and cotton fields infested with Palmer amaranth. In soybean, experiments were established in a field where Palmer amaranth was not adequately controlled with glyphosate in the previous year. Glyphosate applied at 0.87 + 0.87 + 1.74 kg ae ha⁻¹ at 1, 3, and 5 weeks after planting (WAP) provided 82 to 85% control in 2009, but only 23 to 30% control in 2010, a hot and dry year. Glyphosate applied after preemergence (PRE) herbicides improved control to 90 percent. Programs that included s-metolachlor + metribuzin applied preemergence and followed by glyphosate + fomesafen applied postemergence provided the best control (93%) at 8 WAP. Glufosinate based herbicide programs provided greater than 85% control when applied alone, and control increased to 95% when preceded by PRE herbicides. Many conventional control systems integrating different modes of action provided more than 80% control at final evaluation of Palmer amaranth in 2009 and 2010. In soybean, the most consistent and effective program was flumioxazin applied PRE followed by chlorimuron + thifensulfuron, which provided 99 and 82% control at final evaluation in 2009 and 2010, respectively. Cotton fields were heavily infested with Palmer amaranth, but control with glyphosate had historically been good. Glyphosate applied early postemergence, late postemergence, and late post-directed provided more than 95 percent control at final evaluation of Palmer amaranth. Preemergence applications of fomesafen, fluometuron, or pendimethalin + fomesafen provided 77 to 99 percent early-season control and control was complete with an additional postemergence glyphosate application. Glufosinate applied at 0.45 kg ha⁻¹ at 1 and 3 WAP or applied at 0.45 kg ha⁻¹ following a preemergence herbicide provided greater than 95% control. Greenhouse experiments confirmed Palmer amaranth resistance in a population collected from Greensville County, Virginia. In the first experiment, the resistant biotype's I₅₀ value (rate necessary for 50% inhibition) for dry weight was 1.47 kg ae ha⁻¹, which is 4.6 times greater than the susceptible biotype and 1.7 times the recommended use rate of glyphosate. For fresh weight, the I₅₀ value of the resistant biotype was 1.60 kg ae ha⁻¹, 4.7 times that of the susceptible biotype of 0.34 kg ae ha⁻¹. In the second experiment, the I₅₀ value for the susceptible population could not be determined because all glyphosate rates resulted in complete control. However, the resistant population required 1.01 and 1.30 kg ae ha⁻¹ of glyphosate to reduce the fresh and dry weight by 50%. / Master of Science

Master of Science / Department of Agronomy / Johanna A. Dille / No-till producers can manage weeds by including cover crops during the fallow phase as part of an integrated weed management plan. Field experiments were conducted between 2007 and 2009 to quantify the influence of cover crops on weed emergence, biomass accumulation, and seed production. Field experiments were established near Garden City, KS with winter wheat or fallow as main plots and cover crop treatments as subplots including five spring- and five fall-sown individual or mixtures of crop species and a no-cover chemical fallow. Separate1-m2 quadrats were seeded with kochia or downy brome at 500 seed/m2. Kochia density was reduced by 75% and biomass reduced by 88% in fall-sown cover crops compared to chemical fallow across growing seasons. Spring-sown cover crop mixtures reduced kochia biomass in 2009 when kochia emergence was delayed. Downy brome biomass decreased exponentially as cover crop biomass increased. A second field experiment was established near Manhattan, KS with soybean, winter wheat, or grain sorghum phases of the rotation as main plots and six cover crop treatments as subplots sown after winter wheat harvest. Paired Palmer amaranth 1-m2 quadrats were seeded with 500 seed/m2 in each cover crop subplot. One quadrat was protected from any herbicide application made to the cover crop or to the grain sorghum. Combining burndown application with high biomass-producing cover crops reduced Palmer amaranth emergence and biomass. Influence of cover crop presence reduced early season Palmer amaranth emergence in the subsequent grain sorghum phase. Optimal seeding rate of forage soybean sown in winter wheat stubble and its impact on Palmer amaranth and downy brome emergence and growth were evaluated in field studies established near Manhattan and Hesston, KS in 2008 and 2009. Soybean was no-till drilled after wheat harvest at five rates ranging from 100,000 to 600,000 seeds/ha. A no-cover chemical fallow treatment was included. Separate 0.5-m2 quadrats were seeded with Palmer amaranth at 100 seed/0.5 m2 or with downy brome at 250 seed/0.5 m2. Three termination methods evaluated were killing frost, glyphosate application, or crop rolling. Palmer amaranth density was not affected by treatments but biomass decreased as soybean seeding rate and crop biomass increased. Downy brome emergence was less with rolled or sprayed termination methods in one site year as timing of termination was optimal. High biomass producing cover crops sown during the fallow phase of a crop rotation reduced weed emergence, density, and biomass accumulation. Cover crops can be part of an integrated weed management plan in Kansas.

Extensive coastal sabkhas in the northern Gulf of California in North America are colonized by Distichlis palmeri, an endemic perennial grass that produces a grain that was harvested as a staple food by native Cocopah people. Previous short-term trials have shown good vegetative growth but low grain yields. During outdoor trials under anaerobic saline soil conditions of paddy-style irrigation, D. palmeri exhibited high salt tolerance, grain and biomass production. Reproductive maturity was reached four years after initial establishment of plants from seed and a 1:3 mixture of male and female plants produced 231-310 g m⁻² of grain, with nutritional content similar to domesticated grains, confirming the feasibility of developing D. palmeri as a perennial grain and biomass crop for salinized soils and water supplies. Salicornia bigelovii Torr., a cosmopolitan annual coastal marsh succulent, produces seed with high oil content and has been suggested as a potential cash crop for fuel production from saline irrigation but its domestication and development into a cost effective commodity has been slow. A breeding and selection program for agronomic traits that will provide multiple landscape and ecosystem services that could enhance cost benefits of the agronomy of S. bigelovii was initiated during a two year period while producing seed for a pilot system at the Masdar Institute in Abu Dhabi, U.A.E. A concept for a saline landscape designed to consume and concentrate saline waste streams was developed and demonstrates the feasibility and potential to support agronomy of halophytes within a built landscape ecology akin to coastal marsh systems. Exploration and development of potential services halophytes could provide and field testing of selected halophytes for their potential to produce food, fuel, fiber and habitat under designed and managed domestication in our salinized soils with saline waste irrigation needs our continued investigation.

Agave palmeri (Palmer's agave) is a semelparous, perennial succulent thought to provide critical forage for the endangered species, Leptonycteris curasoae (lesser long-nosed bat). Preserving intact agave populations and mitigating loss of habitat may be critical to L. curasoae recovery. Two methods for restoring A. palmeri in disturbed habitats were evaluated: seeding and translocation. In a greenhouse, the emergence and establishment of 2700 seeds was tested across four environmental variables: irrigation level, shade, surface mulch, and soil type. The overall emergence of seedlings was low, particularly in low irrigation, unshaded, unmulched treatments--conditions that might be commonly expected in disturbed habitats. In the field, growth responses of 277 wild transplants were assessed in relation to size class, initial water availability, and storage method. Transplants of all sizes responded positively when replanting coincided with seasonal rainfall, suggesting that salvaging and replanting A. palmeri plants may be a promising restoration strategy.

Crassulacean acid metabolism (CAM) provides desert plants with distinct advantages over the C3 and C4 photosynthetic pathways in harsh climates where water is scarce. CAM is, however more metabolically costly than C3 or C4 photosynthesis, and some plants, such as Mesembryanthemum crystallinum, facultatively utilize CAM when water is abundant, and water conservation unnecessary. In such situations, these plants behave akin to a C3 plant when photosynthesizing. CAM is divided into four phases, with each phase displaying unique metabolic processes. Certain changes, including changes in the timing of CO2 fixation, stable carbon isotope ratios, and tissue malic acid content accumulation patterns can indicate that a plant has shifted from CAM to C3 photosynthesis. Such shifts have been observed to be regulated primarily by water availability and ontogenic development. While facultative CAM is well documented in species like Mesembryanthemum crystallinum, and it has not been studied extensively in Agave with the exception of Agave deserti, and Agave angustifolia. A better understanding of this phenomenon would apply to the agricultural growth of this genus. This study aimed to trigger C3 to CAM shifts in Agave sensu lato species, in order to expand upon the findings of previous studies, and better understand the prevalence of facultative CAM expression in the genus. Gas exchange and stable carbon isotope measurements were taken from 2-month-old, 10-month-old, and mature agaves grown in controlled ocnditions. Tissue acid content measurements were taken from mature plants. Despite the Agave sensu lato species in this study being subjected to moisture applications ranging from dry to saturated, we were unable to observe any distinct shifts from CAM to C3 photosynthesis in any of the species tested for both seedlings and mature plants. Diel net CO2 fixation rates also increased with age, and water applications for seedlings, and decreased with heavy irrigation in mature plants. Stable carbon isotope ratios revealed that some carbon in the plant tissues was fixed by rubisco, and that some species (Polianthes tuberosa, Prochnyanthes mexicana) had carbon isotope ratios of a C3 plant, but these ratios did not change with different irrigation treatments. Malic acid accumulation remained typical of CAM plants for the species tested as well, with one exception in Polianthes tuberosa. As such, we conclude that the Agave sensu stricto species tested in this study are obligate CAM plants, and that they perform poorly mature individuals are over-watered. Additionally, the Agave sensu lato species P. mexicana, and P. tuberosa appear to be C3 plants given the results of this study.