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Prevention of cervical cancer through the characterization of E6 and E7 mRNA transcriptional activity as biological markers of human papillomavirus infectionsTchir, Jayme Dianna Radford 11 1900 (has links)
The quantification of human papillomavirus (HPV) oncogene transcripts, E6 and E7, may be predictive of viral oncogenesis and cancer progression. The main objectives of this study were to determine the HPV genotype prevalence and distribution in Edmonton, Alberta, and characterize a quantifiable association of HPV E6/E7 mRNA expression with the presence of cervical disease. Successful clinical trial design and patient enrolment lead to the first controlled characterization of HPV genotype epidemiology in Alberta. HPV-16 was identified as the most prevalent genotype, followed by several non-vaccine genotypes (HPV-31, -52). Despite rigorous experimentation and a significant correlation between high-risk HPV infection and cervical lesions (p<0.05), absolute quantification of viral oncogenesis was unsuccessful. The ability to quantify oncogene transcriptional activity may, in time, revolutionize cervical cancer screening programmes, akin to the Pap smear several decades ago. However, as experienced in this study and in others, great challenges and even greater questions remain unanswered.
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Human Papillomavirus in human breast cancer and cellular immortalisationKan, Chin Yi, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW January 2007 (has links)
Human Papillomavirus (HPV) is a small, double stranded DNA tumour virus. Infection with HPV normally results in formation of warts. Certain types of HPV, such as type -16 and -18, are shown to have a causal role in the development of uterine cervical cancer, and are so called high risk type HPV. Recently, a role of HPV in breast cancer has been suggested, although a causal role for HPVs in human breast cancer is yet to be demonstrated. The first part of this study investigates the association of HPV with human breast cancer. The results demonstrate that 48% of breast cancers that occurred in Australian women are HPV positive and they are mainly variants of HPV-18. Further analysis shows that HPV positive breast cancer patients are significantly younger than HPV negative patients, suggesting infection with HPV increases the risk of breast cancer development. This is coincidental with increased risk of HPV infection in sexually active young women and provides evidence that HPV has a role in breast cancer development. The second part of this project investigates the mechanisms by which high risk type HPV oncogenic protein E6, transforms primary human foreskin keratinocytes (natural host cells of HPV). HPV E6 is always expressed in HPV positive cervical carcinoma and results in the degradation of the cellular tumour suppressor protein p53. It is generally believed that HPV E6 contributes to HPV transformation by degradation of p53 protein which leads to cellular immortalisation ? an early step in tumorigenic transformation. Subsequent studies, however, indicate that HPV E6 possesses other functions (such as induction of telomerase activity) which may also be involved in cellular immortalisation. The results of my investigations demonstrate: 1) that degradation of p53 protein is required but is insufficient to immortalise primary cells; 2) that HPV E6 induced telomerase activity is coincidental with an increase in cell culture passage number; 3) that multiple functions of high risk type HPV E6 protein are required for cellular immortalisation. This finding suggests HPV infection is associated with early onset of breast cancer and that multiple functions of high risk type HPV E6 protein are involved in cellular immortalisation. Further study in both of these areas should provide alternative diagnostic markers, leading to prevention and treatment strategies for HPV positive breast cancer and other cancers.
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Development of Pichia pastoris as a production system for HPV16 L1 virus-like particles as component to a subunit vaccine /Kotzé, Lara. January 2007 (has links)
Thesis (MScIng)--University of Stellenbosch, 2007. / Bibliography. Also available via the Internet.
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Human papillomavirus (HPV) serum antibodies and their association with clinical manifestations of HPV infection in a cohort of sexually-active women /Shoultz, David Arthur. January 1997 (has links)
Thesis (Ph. D.)--University of Washington, 1997. / Vita. Includes bibliographical references (leaves [114]-135).
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Health beliefs and attitudes of HPV among Hispanic parents as predictors of intention to use the HPV vaccine.Murtaza, Michelle Ruth. Byrd, Theresa, Tarwater, Patrick M. January 2008 (has links)
Thesis (M.P.H.)--University of Texas Health Science Center at Houston, School of Public Health, 2008. / Source: Masters Abstracts International, Volume: 46-05, page: 2669. Adviser: Theresa L. Byrd. Includes bibliographical references.
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Genital human papillomaviruses studies of their occurrence, type spectrum and expression /Forslund, Ola. January 1997 (has links)
Thesis (Ph. D.)--Lund University, 1997. / Added t.p. with thesis statement inserted.
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Characterizing the humoral immune response to human papillomavirus type 6 /Orozco, Johnnie Jose. January 2004 (has links)
Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 72-83).
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Human papillomavirus and oral cancerLiu, Xuan. January 1998 (has links)
Thesis (Ph. D.)--University of California, Los Angeles, 1998. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Natural history of cervical human papillomavirus infections /Roberts, Christine C., January 2002 (has links)
Thesis (Ph. D.)--Lehigh University, 2002. / Includes vita. Includes bibliographical references (leaves 112-125).
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HPV vaccine acceptance among rural, Rocky Mountain region womenAngiola, Julie E. January 2009 (has links)
Thesis (M.S.)--University of Wyoming, 2009. / Title from PDF title page (viewed on May 20, 2010). Includes bibliographical references (p. 32-36).
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