This masters thesis deals with the physiology of binocular vision people for obtaining two dimensional spatial perception of images with glasses. There are developed theoretical part of the physiology of vision, then writing available methods that are now widespread for obtaining stereoscopic scene. The work also describes shooting dynamic scene using a pair of identical cameras. It focuses mainly on OFF-AXES method and deals with the topic of the settings stereoscopic base. There is also realized a practical part, which works with the capture dynamic scenes using video cameras with a automatic control of cameras distance. Automatic control is performed using the parallel port and a stepper motor. For this purpose it is designed to work in hardware support and a program for mutual communication. Filmed a short movie was screened to a group and those observers evaluated the parameters that were specified in the created poll.