Eight foliar insecticide treatment regimes (single applications of Esteem, Danitol + Lorsban, Applaud, Provado and Prev-am, and two applications of Applaud, Provado, and Prev-Am) were evaluated for management of woolly whitefly infestations in grapefruit. All of these products demonstrated efficacy in mitigating woolly whitefly populations. Danitol + Lorsban appeared to be the best knock-down treatment evaluated, but Provado and Prev-Am also demonstrated good activity. For sustained control, all of the treatments were effective; however, Prev-Am required an additional application to achieve equivalent control. Soil injections of 16 and 32 fl-oz/ac of Admire were very effective against WWF, and there were no detectable differences between the two rates. The Admire appeared to require about 27 days after injection to demonstrate consistent activity.

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Orientador: Daniel Júnior de Andrade / Resumo: O ácaro-da-ferrugem-dos-citros Phyllocoptruta oleivora é uma das principais pragas dos citros no Brasil. Os problemas atribuídos a P. oleivora têm se intensificado e suspeita-se que os danos estejam relacionados a uma nova espécie de eriofiídeo descrita recentemente, o ácaro-marrom-dos-citros Tegolophus brunneus. No entanto, não há estudos com essa espécie e informações sobre sua distribuição, características bioecológicas, danos, suscetibilidade a acaricidas e inimigos naturais associados são ausentes. O objetivo principal do projeto foi estudar a distribuição de T. brunneus na principal região citrícola brasileira e sobre as principais espécies e variedades cítricas. Além disso, estudou-se a biologia de T. brunneus em laboratório, determinou-se a suscetibilidade desse ácaro aos principais acaricidas, caracterizando seus danos em plantas cítricas, bem como o potencial de predação das principais espécies de predadores associadas ao ácaro e seu potencial para o uso no controle biológico. As coletas realizadas em vários municípios do estado São Paulo e Triângulo Mineiro demonstraram que T. brunneus infestou apenas lima ácida ‘Tahiti’, enquanto P. oleivora infestou todas as outras espécies e variedades cítricas. O ácaro completa o desenvolvimento em 7 dias, com período de incubação de 3 dias, duração de larva de 2,1 dias e de ninfa 2,8 dias e as fêmeas apresentam fecundidade de 8,5 ovos a 25°C. Os danos da espécie caracterizam-se pelo prateamento dos frutos e formação de manchas... (Resumo completo, clicar acesso eletrônico abaixo) / Doutor

In the management of agricultural insect pests, short-term costs must be balanced against long-term benefits. Controls should be selected to account for both their immediate and downstream effects upon the demography and genetics of the pest, enabling suppression today without threatening suppression tomorrow. The iterative, algorithmic method of dynamic programming can provide optimal solutions to problems of this type, in which actions are taken sequentially and each action may influence those which follow it. However, this approach is fundamentally constrained with regards to the magnitude of the problems it may solve. As questions of insect pest management can be subject to ecological and evolutionary complexities, this may place them beyond the scope of dynamic programming. When it is the intricacies of a problem that are of interest, it may be more productive to utilise approximate dynamic programming (ADP) methods which can attempt problems of arbitrary complexity, although at the expense of no longer guaranteeing optimality. In this thesis I first challenge a dynamic programming algorithm with the management of a hypothetical insect pest feeding upon a transgenic insecticidal crop. The model explores how different realisations of fitness costs to resistance influence the algorithms suggested actions. I then apply a brute-force variant of ADP, a lookahead policy, to the management of a stage-structured, continuously reproducing pest population. This was to explore the extent to which an algorithm with a limited temporal perspective is able to balance the timetable of pest demography against the timescale over which insecticidal sprays and bisex-lethal sterile insect releases unfold. This same decision framework is then applied to a modified problem in which resistance to insecticidal toxins may evolve and releases are now male-selecting. This was used to assess the efficacy with which simple lookahead policies utilise a control with delayed benefits (the male-selecting releases) and possible constraints on their capacity to respond to resistance evolution. Dynamic programming and ADP methods offer a versatile toolbox for accounting for the potential impacts of the evolutionary and ecological peculiarities of particular pests upon control decisions.

Orientador: Carlos Gilberto Raetano / Banca: Vilson Luís Kunz / Banca: Fernando Cesar Bauer / Banca: Marcelo da Costa Ferreira / Banca: Carlos Frederico Wilcken / Resumo: O controle químico de pragas e doenças é uma prática imprescindível no manejo fitossanitário do algodoeiro para a redução das perdas de produtividade. Com o intuito de promover o uso racional dos produtos fitossanitários nessa cultura, esse trabalho teve por objetivo comparar três tecnologias de pulverização: assistida por ar, assistida por ar associada a gotas com carga elétrica e convencional (sem ar e carga elétrica) combinadas aos volumes de 50 e 100L.ha-1, sobre a deposição e cobertura da pulverização, controle de insetos pragas e doenças e produtividade da cultura do algodão. Os experimentos foram conduzidos no delineamento de blocos ao acaso com seis tratamentos e quatro repetições para avaliação da deposição, cobertura e controle de pragas e doenças. As pulverizações foram realizadas em dois estádios de desenvolvimento das plantas conduzidas nos espaçamentos de 0,45 m e 0,90 m entre linhas de semeadura da cultura. Para isso, utilizou-se um pulverizador autopropelido equipado com pontas de pulverização de jato cônico vazio, JA-1 (50L.ha-1) e JA-2 (100L.ha-1) na pressão de 828 kPa. Foi avaliado o efeito das respectivas tecnologias no controle de Pseudoplusia includens, Bemisia tabaci, Aphis gossypii, Spodoptera spp. e Thrips spp. e da doença fúngica Ramularia areola durante todo o ciclo de desenvolvimento da cultura, bem como a produtividade do algodoeiro no sistema de cultivo convencional. As dimensões das parcelas foram 24 m de largura x 100 m de comprimento em ambos os sistemas de semeadura, para os ensaios de deposição e cobertura. Para avaliação da deposição e cobertura foram escolhidas, ao acaso, 10 plantas e, de cada uma delas removida uma folha do ápice e outra folha da base da planta / Abstract: Chemical control has been an essential practice on the pest management of cotton crop to reduce productivity losses. In order to promote rational use of pesticides, this work was aimed to compare three spray technologies: air-assisted, assisted by air associated with electrically charged droplets and conventional spraying (without air and electric charge) combined with volume rates of 50 and 100L.ha-1, on spray deposition, coverage and in the effect of control insect pests and disease. The trials were conducted in a randomized blocks design with six treatments and four replications for evaluation of the deposition, coverage and effect on pests and disease. The spraying was carried out on two growth stages of plants conducted in spacing of 0.45 m and 0.90 m between plant rows of culture. For this, were used a self-propelled sprayer equipped with spray nozzles hollow cone, JA-1 (50L.ha-1) and JA-2 (100L.ha-1) at 828 kPa of work pressure. The effect of these technologies was evaluated on controlling of Pseudoplusia includens, Bemisia tabaci, Aphis gossypii, Spodoptera spp. and Thrips spp. and a fungal disease Ramularia areola all the development cycle of the culture as well as the yield in conventional tillage. The dimensions of the plots were 24 m wide x 100 m length in both sowing systems for deposition and coverage tests. To evaluate the deposition and coverage were selected at random 10 plants and, of each one removed one leaf from the apex and another leaf of the lower part of plant. The tracer dye Brilliant Blue FD & C No. 1 (0.3%), fluorescent pigment ColorLight (0.15%) and Lignosulfonate Vixilperse (0.015%) were the tracer mixture. In experiments in which we evaluated the effect of the treatments on controlling pests and disease, were installed plots of 24 m wide x 250 m length and then weekly samplings were carried out as required spraying / Doutor

Delia radicum L. (Diptera: Anthomyiidae), the cabbage root fly, is a specialist root-feeding insect pest of Brassica crops. The impending withdrawal of chlorpyrifos, one of the main pesticides used against D. radicum, opens new opportunities to research alternative pest management strategies. Manipulating host plant location cues to influence D. radicum adult and larval behaviour, along with induced plant defence responses, offer potential integrative crop protection solutions (Chapter 1). This thesis aimed to identify the semiochemistry underpinning D. radicum larval host plant location, and to investigate whether plant defence induction treatments (methyl jasmonate [MeJA], D-Fructose) and a herbivore induced volatile (dimethyl disulfide [DMDS]) affect D. radicum larval performance and adult oviposition preference. In choice-test bioassays, larvae were inconsistent in their responses to root exudates collected from Brassica host plants (Chapter 2). A combined bioassay and EthoVision® video-tracking approach was developed to record and analyse larval movements in response to volatiles emitted from host and non-host plant roots (Chapter 3). Larvae were significantly attracted to host plant root volatiles. Olfactory stimuli from roots of the non-host plant onion (Allium cepa L. ‘Ailsa Craig’), which share overlapping, yet distinctive volatile profiles to that of Brassica plants, also elicited positive taxis. By analysing the volatile metabolome of broccoli (B. oleracea L. convar. botrytis L. Alef. var. cymosa Duchesne ‘Parthenon’) and onion roots using solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GCMS), a suite of candidate volatile orientation cues were identified. A SPME-based method was developed to non-invasively collect root volatiles in situ from glasshouse- and field-grown broccoli plants pre- and post-D. radicum infestation (Chapters 4, 5 and 6). GC-MS analyses revealed that sulfur compounds, showing characteristic temporal emission patterns, were the principal volatiles released by roots in response to damage. This new method, which has potential for wide application in chemical ecology research, allows the study of volatiles in the soil in situ that are critical for interactions between trophic levels. In EthoVision® bioassays, a major volatile constituent of broccoli roots, DMDS, was attractive to larvae, but toxic at the highest dose tested (Chapter 3). Glasshouse and on-farm experiments using broccoli were conducted to evaluate the efficacy of MeJA, D-Fructose and DMDS against D. radicum compared to commercially available crop protection products (chlorpyrifos [Dursban® WG], spinosad [Tracer®], Steinernema feltiae Filipjev [Nematoda: Steinernematidae] [Entonem] and garlic granules [ECOguard®]). MeJA and garlic reduced larval performance under glasshouse conditions whereas D-Fructose and DMDS did not at the concentrations tested (Chapter 7). In field studies, MeJA combined with reduced rate chlorpyrifos, spinosad, and S. feltiae all showed partial efficacy for controlling D. radicum larvae. Inherent field site, weather and D. radicum population density variability highlighted that glasshouse results cannot always be reproduced in more complex field environments (Chapter 8). Further research is needed into formulation, mode of application and timing to improve efficacy of promising treatments that may help in future integrated pest management (IPM) for this key pest in the absence of existing pesticides.

Inclusion/exclusion field experiments demonstrated that the red imported fire ant, Solenopsis invicta Buren, did not affect the abundance of 49 groups of insects and spiders collected in pitfall traps. However, arthropod diversity was significantly greater (H' = 2.829) in exclusion plots relative to inclusion plots (H' = 2.763). Moreover, this study demonstrated that S. invicta can have an important impact upon cotton arthropod communities, including key predator species. Densities of ground beetles (Carabidae), spiders, lacewings (Chrysoperla spp.), and minute pirate bugs (Orius spp.) were significantly lower in the presence of S. invicta. However, populations of aphidophagous insects such as Hippodamia spp. and Scymnus spp. increased with cotton aphid (Aphis gossypii Glover) density early in the season. Abundance of cotton aphids was ca. 5x greater in inclusion plots, likely due to protection and tending by S. invicta. This increase was observed early in the season, though aphid populations did not reach economic levels. Predation of sentinel bollworm [Helicoverpa zea (Boddie)] and beet armyworm (Spodoptera exigua Hubner) eggs increased 20-30%, when S. invicta was present. Most predation of sentinel beet armyworm egg masses, measured via direct nocturnal observations, was due to S. invicta (68%) and cotton fleahopper [Pseudatomoscelis seriatus (Reuter)] (21%) in plots with S. invicta, and by the mite Abrolophus sp. (52%), spiders (13%) and minute pirate bug (13%) in plots without S. invicta. The frequencies of minute pirate bug, cotton fleahopper, S. invicta and native ants in beat bucket samples did not accurately reflect the frequency with which they were observed feeding on sentinel noctuid eggs. Overall, the results of these studies demonstrated that S. invicta was associated with declines in the abundances of minute pirate bug, spiders, and lacewing and with an increment in cotton aphid populations, though they did not reach the economic threshold. Moreover, S. invicta significantly increased predation of bollworm and beet armyworm eggs. All together, the results suggested that S. invicta has a net positive impact on cotton pest management.

11 pp. / This document provides a general overview of bat biology and behavior with emphasis on urban environments, use of integrated pest management (IPM) techniques that are in keeping with bat conservation guidelines, and disease awareness and prevention efforts.

31 pp. / Many pests encountered in homes and structures can be prevented by using simple techniques collectively known as “pest-proofing”. If done correctly, pest-proofing your home saves you money by reducing pest management costs, and more importantly, reduces potential pesticide exposure. This publication describes general indoor and outdoor pest-proofing measures and some of the major pests encountered in and around homes and structures.

Inclusion/exclusion field experiments demonstrated that the red imported fire ant, Solenopsis invicta Buren, did not affect the abundance of 49 groups of insects and spiders collected in pitfall traps. However, arthropod diversity was significantly greater (H' = 2.829) in exclusion plots relative to inclusion plots (H' = 2.763). Moreover, this study demonstrated that S. invicta can have an important impact upon cotton arthropod communities, including key predator species. Densities of ground beetles (Carabidae), spiders, lacewings (Chrysoperla spp.), and minute pirate bugs (Orius spp.) were significantly lower in the presence of S. invicta. However, populations of aphidophagous insects such as Hippodamia spp. and Scymnus spp. increased with cotton aphid (Aphis gossypii Glover) density early in the season. Abundance of cotton aphids was ca. 5x greater in inclusion plots, likely due to protection and tending by S. invicta. This increase was observed early in the season, though aphid populations did not reach economic levels. Predation of sentinel bollworm [Helicoverpa zea (Boddie)] and beet armyworm (Spodoptera exigua Hubner) eggs increased 20-30%, when S. invicta was present. Most predation of sentinel beet armyworm egg masses, measured via direct nocturnal observations, was due to S. invicta (68%) and cotton fleahopper [Pseudatomoscelis seriatus (Reuter)] (21%) in plots with S. invicta, and by the mite Abrolophus sp. (52%), spiders (13%) and minute pirate bug (13%) in plots without S. invicta. The frequencies of minute pirate bug, cotton fleahopper, S. invicta and native ants in beat bucket samples did not accurately reflect the frequency with which they were observed feeding on sentinel noctuid eggs. Overall, the results of these studies demonstrated that S. invicta was associated with declines in the abundances of minute pirate bug, spiders, and lacewing and with an increment in cotton aphid populations, though they did not reach the economic threshold. Moreover, S. invicta significantly increased predation of bollworm and beet armyworm eggs. All together, the results suggested that S. invicta has a net positive impact on cotton pest management.