Thesis (Ph. D.)--University of Wisconsin--Madison, 1955. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 83-87).

Thesis (Ph. D.)--University of Hong Kong, 1996. / Includes bibliographical references (leaf 140-157).

Thesis (Ph. D.)--University of Virginia, 2000. / Spine title: Role of REGL in glucose repression. Includes bibliographical references (p. 194-220). Also available online through Digital Dissertations.

Thesis (M. Phil.)--University of Hong Kong, 2009. / Includes bibliographical references (leaves 112-131) Also available online.

Thesis (Ph. D.)--University of Washington, 2005. / Vita. Includes bibliographical references (leaves 133-144).

Thesis (Ph.D.) - University of Queensland, 2003. / Includes bibliography.

Thesis (Ph. D.)--Ohio State University, 2005. / Available online via OhioLINK's ETD Center; full text release delayed at author's request until 2006 Nov 30

Thesis (Ph.D)--Boston University / The purpose of the research was to investigate the action of prostatic acid phosphatase on a spectrum of physiologically significant phosphate esters under varying environmental conditions. The effects of varying pH, substrate concentration, and enzyme concentration, the influence of inhibitors, and the influence of time on enzymatic hydrolysis were studied with each substrate. The enzymes extracted from both normal and cancerous prostatic tissue were used in an attempt to discover any differences existing between enzymes. A survey of the literature was made. A historical review of the history of acid phosphatase, its distribution in animal tissues and the influence of experimental conditions on enzyme activity is included in the body of the dissertation. Little work has been done to investigate the action of acid phosphatases on physiologically significant substrates, and the comparison of normal and cancerous enzyme preparations from human tissues has attracted the attention of only several investigators. [TRUNCATED]

The response of Pseudomonas aeruginosa to phosphate starvation and subsequent refeeding was studied by following changes in turbidity, cell count and chemical composition on incubation in phosphate deficient medium. In shaken, phosphate deficient cultures, the turbidity and viable cell count were shown to increase significantly, with the latter reaching a maximum level at 24 hours under the conditions employed. A linear response of phosphate starved cells to low levels of phosphate supplied exogenously was evident from turbidity measurements, and a threshold requirement for phosphate analogous to the "energy of maintenance" (McGrew and Mallette, 1962) was not detected. In still, phosphate deficient cultures, the turbidity and total cell count increased and the viable cell count decreased slightly at 24 hours. The levels of protein and deoxyribonucleic acid (DNA) per ml of culture increased during this period, and the amount of ribonucleic acid (RNA) decreased. Extensive ribosomal degradation was apparent from sucrose density gradient centrifugation patterns. An enzyme having an alkaline pH optimum and displaying activity against a wide variety of phosphomonoesters was demonstrated in phosphate-starved cells. The enzyme was inhibited by inorganic phosphate, and was considered to be the counterpart of the repressible phosphomono-esterase reported in other microorganisms and studied in detail in Escherichia coli (Torriani, i960; Garen and Levinthal, I960; Heppel, Harkness and Hilmoe, 1962). The enzyme activity of cell free extracts of P. aeruginosa was shown to be associated mainly with the ribosomal fraction. / Land and Food Systems, Faculty of / Graduate

Soybean (Glycine max) and rice (Oryza sativa) are the most important crops cultivated worldwide. The productivity of both crops is severely limited due to drought stresses. Abscisic acid (ABA) signaling is one of the crucial phytohormones which acts as the signaling mediator in different environmental stress for adaptive response of plants. In this study, functional characterization of abscisic acid-activated protein kinase-like kinase 1 (AALK1), and low molecular weight protein tyrosine phosphatase (LMWPTP) were studied by developing gain-ofunction and loss-ofunction phenotypes by transgenesis. Physiological response of AALK1 showed that AALK1 modulates the drought stress response ins soybean plants. The study has demonstrated several key genes are differentially expressed control, and aalk1-RNAi silenced lines under drought treatment. The AALK1 overexpression lines enhanced the transcription of other ABA-responsive genes, indicating that the AALK1 is a positive regulator of ABA-mediated stress signaling pathways in soybean. The phylogenetic analysis and domain analysis also supports that AALK1 is abscisic acid-activated protein kinase and has a role in drought response. Phenotype analysis of LMWPTP in rice showed that transgenic overexpression of LMW-PTP exhibited significantly improved drought tolerance in comparison to RNAi silencing and control plants ,which indicates that LMWPTP modulates the drought stress tolerance of rice plants. Further, 5 putative tyrosine phosphorylated proteins were detected through immunoblotting and identified by mass spectrometry. Some of these tyrosine phosphorylated proteins are likely to be target proteins of LMWPTP. Together, the present findings strengthen the knowledge about the functional role of AALK1 and LMWPTP, which can be utilized as a promising gene-based molecular marker in transgenic breeding for generating crop plants with improved drought tolerance which ultimately improve the grain yields.